Laccase immobilized on magnetic nanoparticles by dopamine polymerization for 4-chlorophenol removal

作     者：Di Zhang Manfeng Deng Hongbin Cao Songping Zhang He Zhao 

作者机构：Beijing Engineering Research Center of Process Pollution Control Division of Environment Technology and Engineering Institute of Process EngineeringChinese Academy of Sciences Collaborative Innovation Center of Chemical Science and Engineering State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences 

出 版 物：《Green Energy & Environment》 (绿色能源与环境（英文版）)

年 卷 期：2017年第2卷第4期

页      面：393-400页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：support from the National Natural Science Foundation of China(Grant Nos.51378487,51425405,21376249,21336010) Youth Innovation Promotion Association,CAS(2014037) 973 Program(2013CB733604) 

主　　题：Magnetic nanoparticle Laccase Dopamine Immobilization 4-Chlorophenol removal 

摘      要：In this work, a new immobilization method based on dopamine(DA) self-polymerization was developed for laccase immobilization on magnetic nanoparticles(Fe_3O_4 NPs). To optimize the immobilization condition including reaction pH, DA concentration and enzyme concentration, a central composite response surface method was applied. The optimal condition was determined as p H value of 5.92, laccase concentration of 0.25 mg mL^(-1) and DA concentration of 12.74 mg mL^(-1), under which a high enzyme activity recovery of 88.17% was *** comparing with free laccase, the stabilities of immobilized laccase towards p H, thermostability, storage were enhanced *** 60% of relative activity for immobilized laccase was remained after being incubated for 6 h at 50℃, but the free laccase only remained 25%. After 40 days of storage at 4℃, the laccase immobilized by DA kept about 89% of its original activity, but the free laccase only retained 48%. After recycled 10 times, the relative activity of immobilized laccase still retained 70%. The immobilized laccase was then applied to catalyze the degradation of 4-chlorophenol(4-CP), 86% percentage of 4-CP was removed within 2 h. After degraded 10 times, the relative activity of immobilized laccase still remained 64% of its initial activity, which exhibits an excellent reusability and operational stability.