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Detection of β-Glucuronidase Activity within Actinomadura madurae Grains of Human Actinomycetoma

Advances in Microbiology 2014年第6期4卷 317-323页

作者： Alejandro Palma-Ramos Samantha Reyes-Mayén laura Estela Castrillón-Rivera silvia elena fernández-lópez Carmen Padilla-Desgarennes María Elisa Vega-Memije Roberto Arenas-Guzmán María Elisa Drago-Serrano Teresita Sainz-Espunes Department of Biological Systems Universidad Autónoma Metropolitana-XochimilcoMexico CityMéxico Dermatology Center“Dr.ladislao de la Pascua” Mycology ServiceMexico City Health SecretaryMexico CityMéxico General Hospital“Manuel Gea González” Dermatology ServiceMexico City Health SecretaryMexico CityMéxico General Hospital“Manuel Gea González” Mycology ServiceMexico City Health SecretaryMexico CityMéxico

Actinomycetoma syndrome by Actinomadura (A.) madurae is characterized by a subcutaneous chronic lesion that affects fascia, muscle and bone. A. madurae produces colonies that form grains of less than 1 mm in diameter. Grains are surrounded and infiltrated by neutrophils involved in the grain disruption by enzymes like β-glucuronidase released after the neutrophil degranulation. The aim of this work was to evaluate the polysaccharide degradation of grains treated with β-glucuronidase and to detect the presence and activity of β-glucuronidase within the A. madurae grains. Actinomadura madura grains from patients infected were processed to quantify the total content of polysaccharide with the phenol-sulfuric acid reaction. Grains were treated with β-glucuronidase at different conditions to evaluate the optimal polysaccharide degradation. Grains were analyzed to detect the enzyme by using anti-human β-glucuronidase antibody while enzymatic activity was assessed by evaluating the release of reduced sugars and by in situ enzymatic activity. Optimal degradation of polysaccharide in the grains treated with β-glucuronidase was found with 300 units/ml of enzyme and 24 hr of incubation at 37°C. Presence and activity of β-glucuronidase enzyme within the grains were detected. Results suggested that β-glucuronidase present within A. madurae grain resulted from degranulated neutrophils surrounding and/or infiltrated within the grain.

关键词： Actinomycetoma A. madurae β-Glucuronidase

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大陆俯冲界面之上大规模硅富集流体的地球物理学证据

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中国科学:地球科学 2024年第9期54卷 2848-2863页

作者：毛元彤赵亮 Marco G.MAlUSà Stefano SOlARINO silvia PONDREllI 孙宝璐 Coralie AUBERT Simone SAlIMBENI elena EVA Stéphane GUIllOT 中国科学院地质与地球物理研究所岩石圈演化与环境演变全国重点实验室中国科学院大学地球与行星科学学院 Department of Earth and Environmental Sciences University of Milano-Bicocca Istituto Nazionale di Geofisica e Vulcanologia Osservatorio Nazionale Terremoti Istituto Nazionale di Geofisica e Vulcanologia Sezione di Bologna Université Grenoble Alpes Université Savoie Mont BlancCNRSIRDUGEISTerre

俯冲带中的流体可能对俯冲过程产生重要影响.然而,关于大陆俯冲期间流体规模及其作用的地球物理约束仍然有限.西阿尔卑斯造山带是全球保存最完好的大陆俯冲带之一,为认识大陆俯冲过程中流体作用的影响提供了重要的参考.本文综合多种地... 详细信息

俯冲带中的流体可能对俯冲过程产生重要影响.然而,关于大陆俯冲期间流体规模及其作用的地球物理约束仍然有限.西阿尔卑斯造山带是全球保存最完好的大陆俯冲带之一,为认识大陆俯冲过程中流体作用的影响提供了重要的参考.本文综合多种地球物理方法分析了西阿尔卑斯造山带地区的VP/VS比值,发现在俯冲区的上盘侧存在高VP/VS（>1.9）带,与部分蛇纹岩化的上覆地幔楔位置一致;而在下盘侧存在异常低的VP/VS（<1.7）带,异常深度浅于30km.本文认为,观测到的低VP/VS比值异常是由地壳内广泛分布的富硅岩脉导致,指示了在俯冲时期大陆俯冲界面之上可能存在的富硅流体流动.本文的结果暗示俯冲时期的流体流动可能降低了俯冲界面的有效应力,从而促进了大陆俯冲.

关键词：西阿尔卑斯造山带地壳精细结构接收函数 VP/VS波速比硅富集

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Geophysical evidence of large-scale silica-rich fluid flow above the continental subduction interface

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Science China Earth Sciences 2024年第9期67卷 2796-2809页

作者： Yuantong MAO liang ZHAO Marco G.MAlUSà Stefano SOlARINO silvia PONDREllI Baolu SUN Coralie AUBERT Simone SAlIMBENI elena EVA Stéphane GUIllOT State Key laboratory of lithospheric and Environmental Coevolution Institute of Geology and GeophysicsChinese Academy of Sciences College of Earth and Planetary Sciences University of Chinese Academy of Sciences Department of Earth and Environmental Sciences University of Milano-Bicocca Istituto Nazionale di Geofisica e Vulcanologia Osservatorio Nazionale Terremoti Istituto Nazionale di Geofisica e Vulcanologia Sezione di Bologna Université Grenoble Alpes Université Savoie Mont BlancCNRSIRDUGEISTerre

Fluids in subduction zones can have major effects on subduction dynamics. However, geophysical constraints on the scale and impact of fluid flow during continental subduction are still limited. Here we analyze the VP/VSratios in the Western Alpine region, hosting one of the best-preserved fossil continental subduction zones worldwide, to investigate the impact of fluid flow during continental subduction. We found a belt of high VP/VSratios >1.9 on the upper-plate side of the subduction zone,consistent with a partially serpentinized upper-plate mantle, and a belt of unusually low VP/VSratios lower-plate side,at depths shallower than 30 km. We propose that these low VP/VSratios result from a widespread network of silica-rich veins,indicating past fluid flow along the continental subduction interface. Our results suggest that past fluid flow may have reduced the effective stress along the subduction interface thus favoring continental subduction.

关键词： Western Alps Crustal structure Receiver function VP/VS ratio Silica enrichment

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Enhancing sensitivity of triple-negative breast cancer to DNA-damaging therapy through chemical inhibition of the m6A methyltransferase METTl3

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Cancer Communications 2024年第2期44卷 282-286页

作者： Bianca Cesaro Alessia Iaiza Fabio Piscopo Marco Tarullo Eleonora Cesari Dante Rotili Antonello Mai Alberto Diana Michela londero luca Del Giacco Riccardo Masetti Alba Di leone Chiara Naro silvia Masciarelli Giulia Fontemaggi Claudio Sette Francesco Fazi Alessandro Fatica Department of Anatomical HistologicalForensic&Orthopedic SciencesSection of Histology&Medical EmbryologySapienza University of RomeRomeItaly Department of Biology and Biotechnologies“Charles Darwin” Sapienza University of RomeRomeItaly GSTeP Organoids Research Core Facility Fondazione Policlinico A.Gemelli Istituto di Ricovero e Cura a Carattere Scientifico(IRCCS)RomeItaly Department of Drug Chemistry and Technologies Sapienza University of RomeRomeItaly Pasteur Institute Cenci-Bolognetti FoundationSapienza University of RomeRomeItaly Department of BioSciences University of MilanMilanItaly Multidisciplinary Breast Center Dipartimento Scienze della Salute della Donna e del Bambino e di SanitàPubblicaCatholic University of the Sacred HeartRomeItaly Department of Neuroscience Section of Human AnatomyCatholic University of the Sacred HeartRomeItaly Oncogenomic and Epigenetic Unit IRCCS Regina Elena National Cancer InstituteRomeItaly

Dear Editor,N 6-methyladenosine(m^(6)A)is a critical mRNA modi-fication catalyzed by the enzyme methyltransferase-like 3(METTl3),with implications in RNA ***3 upregulation is associated with cancer progres-sion,metast... 详细信息

关键词： cancer methyltransferase metastasis

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ACE Score Identifies HBeAg-negative Inactive Carriers at a Single-point Evaluation,Regardless of HBV Genotype

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Journal of Clinical and Translational Hepatology 2022年第6期10卷 1068-1076页

作者： luisa Roade Mar Riveiro-Barciela Adriana Palom Francisco Rodríguez-Frías Marta Bes Ariadna Rando María Teresa Salcedo Rosario Casillas elena Vargas-Accarino David Tabernero silvia Sauleda Rafael Esteban María Buti liver Unit Internal Medicine DepartmentHospital Universitari Vall d’HebronVall d’Hebron Barcelona Hospital CampusBarcelonaSpain Universitat Autònoma de Barcelona(UAB) Department of MedicineBarcelonaSpain Universitat Au-tònoma de Barcelona(UAB) Department of Biochemistry and Molecular BiologyBarcelonaSpain Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas(CIBERehd) Instituto de Salud Carlos IIIMadridSpain liver Pathology lab Biochemistry and Microbiology Departments(Clinical Laboratories)Hospital Universitari Vall d’HebronVall d’Hebron Barcelona Hospital CampusBarcelonaSpain Vall d’Hebron Institut de Recerca(VHIR) Liver Diseases GroupBarcelonaSpain Transfusion Safety laboratory Banc de Sang i TeixitsServei Catalàde la SalutBarcelonaSpain De-partment of Pathology Hospital Universitari Vall d’HebronUniversitat Autònoma de BarcelonaBarcelonaSpain

Background and Aims:Hepatitis B virus(HBV)biomark-ers have been used for a better categorization of patients,even though the lack of simple algorithms and the impact of genotypes limit their *** aim was to assess the usefulness of noninvasive markers for the identification of HBV inactive carriers(ICs)in a single-point evaluation and to design a predictive model for their ***-ods:This retrospective-prospective study included 343 consecutive HBeAg-negative ***,analytical,and virological data were collected,and a liver biopsy was performed if *** were classified at the end of follow-up as ICs,chronic hepatitis B and gray zone.A pre-dictive model was constructed,and validated by 1000-boot-strap ***:After 39 months of follow-up,298 subjects were ICs,36 were chronic hepatitis B CHB,and nine were gray ***-nine(25.9%)individuals re-quired a liver *** HBV DNA hazard ratio(HR)6.0,plated antigen(HBcrAg)(HR 6.5,plastography(HR 4.6,ply associated with the IC *** ACE score(HBV DNA,HBcrAg,elastography),obtained by bootstrapping,yielded an area under the receiver operating characteris-tics(AUROC)of 0.925(95%CI:0.880-0.970,plue of 99.5%,and a score≤12 points had a diagnostic accuracy of 93.8%.Conclusions:low baseline HBV DNA,HBcrAg,and liver stiffness were in-dependently associated with the IC phase.A score including those variables identified ICs at a single-point evaluation,and might be applied to implement less intensive follow-up strategies.

关键词： Hepatitis B virus Inactive carrier liver stiffness HBV DNA Quan-titative HBsAg Core-related antigen.

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Regulation by l Calcium Channels of Hypoxia-Elicited Quantal Catecholamine Release and Excitability in Rat Embryo Chromaffin Cells

Regulation by L Calcium Channels of Hypoxia-Elicited Quantal...

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第十六届嗜铬细胞国际学术会议

作者： Antonio M G de Diego Jose Carlos fernndez silvia lorrio elena Díaz Antonio G.García Universidad Autónoma de Madrid Universidad de Valencia

＜正＞We investigated the relative contribution to hypoxia induced secretion（HIS） of voltageactivated calcium channels（VACCs） in 18-days old rat embryo chromaffin cells（ECCs）.l,N and PQ channels were present in ECCs in the same proportion than in the adult mother chromaffin cell（MCC） as revealed through patch-clamp measurement of calcium currents

关键词： hypoxia embryo amperometry patch-clamp lDCV

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Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration

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Journal of Environmental Sciences 2013年第3期25卷 613-625页

作者： Melina Nisenbaum Gonzalo Hernn Sendra Gastón Alfredo Cerd Gilbert Marcelo Scagliola Jorge Froiln Gonzlez silvia elena Murialdo National University of Mar del Plata Faculty of EngineeringDepartment of ChemistryBiochemical Engineering Group.Juan B.Justo 4302(7600)Mar del PlataArgentina National University of Mar del Plata Faculty of EngineeringLaser Laboratory7600Mar del PlataArgentina and Optical Research CenterCONICETLa Plata-CIC.PO Box No.3 1897GonnetLa PlataArgentina OSSE.Obras Sanitarias Mar del Plata-Batn S.E.Quality Management Water Laboratory.French 67377600Mar del PlataArgentina

We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader,Pseudomonas strain *** biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with *** *** strain was able to degrade n-hexadecane,1-undecene,1-nonene,1-decene,1-dodecene and *** grew in the presence of 1-octene,while this hydrocarbons is toxic to other hydrocarbons *** strain H was also chemotactic towards n-hexadecane,kerosene,1-undecene and *** results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled *** the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations,we demonstrate the use of the dynamic speckle laser method,which is simple and inexpensive,to confirm bacterial chemotaxis at low cell concentrations （less than 105 colony-forming unit per millilitre （CFU/ml）） when hydrocarbons are the attractants.

关键词： dynamic laser speckle microorganisms biodegradation chemotaxis hydrocarbons

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Physical energies to the rescue of damaged tissues

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World Journal of Stem Cells 2019年第6期11卷 297-321页

作者： Federica Facchin silvia Canaider Riccardo Tassinari Chiara Zannini Eva Bianconi Valentina Taglioli elena Olivi Claudia Cavallini Marco Tausel Carlo Ventura Department of Experimental Diagnostic and Specialty Medicine (DIMES)School of MedicineUniversity of BolognaBologna 40100Italy National laboratory of Molecular Biology and Stem Cell Engineering National Institute of Biostructures and BiosystemsCNRBologna 40100Italy iB3 SA Lugano 6900Switzerland

Rhythmic oscillatory patterns sustain cellular dynamics, driving the concerted action of regulatory molecules, microtubules, and molecular motors. We describe cellular microtubules as oscillators capable of synchronization and swarming, generating mechanical and electric patterns that impact biomolecular recognition. We consider the biological relevance of seeing the inside of cells populated by a network of molecules that behave as bioelectronic circuits and chromophores. We discuss the novel perspectives disclosed by mechanobiology, bioelectromagnetism, and photobiomodulation, both in term of fundamental basic science and in light of the biomedical implication of using physical energies to govern (stem) cell fate. We focus on the feasibility of exploiting atomic force microscopy and hyperspectral imaging to detect signatures of nanomotions and electromagnetic radiation (light), respectively, generated by the stem cells across the specification of their multilineage repertoire. The chance is reported of using these signatures and the diffusive features of physical waves to direct specifically the differentiation program of stem cells in situ, where they already are resident in all the tissues of the human body. We discuss how this strategy may pave the way to a regenerative and precision medicine without the needs for (stem) cell or tissue transplantation. We describe a novel paradigm based upon boosting our inherent ability for self-healing.

关键词： Stem cells Physical energies Mechanical forces Electric fields Electromagnetic fields Electromagnetic radiation Photobiomodulation Damaged tissues

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Quality control methods in musculoskeletal tissue engineering:from imaging to biosensors

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Bone Research 2021年第4期9卷 473-493页

作者： Daniele Zuncheddu elena Della Bella Andrea Schwab Dalila Petta Gaia Rocchitta silvia Generelli Felix Kurth Annapaola Parrilli Sophie Verrier Julietta V.Rau Marco Fosca Margherita Maioli Pier Andrea Serra Mauro Alini Heinz Redl Sibylle Grad Valentina Basoli AC Research Institute Davos Clavadelerstrasse 87270 DavosSwitzerland Regenerative Medicine Technologies lab Ente Ospedaliero Cantonale(EOC)Via Tesserete 466900 LuganoSwitzerland Department of Medical Surgical and Experimental SciencesUniversity of SassariViale San Pietro 43/b07100 SassariItaly Centre Suisse d'Electronique et de Microtechnique Bahnhofstrasse 17302 LandquartSwitzerland Center for X-ray Analytics Empa-Swiss Federal Laboratories for Materials Science and TechnologyUberlandstrasse 1298600 DubendorfSwitzerland lstituto di Struttura della Materia Consiglio Nazionale delle Ricerche(ISM-CNR)Via del Fosso del Cavaliere100-00133 RomeItaly Sechenov First Moscow State Medical University Trubetskaya 8build.2119991 MoscowRussian Federation Department of Biomedical Sciences University of SassariViale San Pietro 43/b07100 SassariItaly ludwig Boltzmann Institute for Experimental and Clinical Traumatology in AUVA trauma research center DonaueschingenstraBe 131200 ViennaAustria Austrian Cluster for Tissue Regeneration ViennaAustria

Tissue engineering is rapidly progressing toward clinical *** the musculoskeletal field,there has been an increasing necessity for bone and cartilage *** the promising translational potential of tissue engineering approaches,careful attention should be given to the quality of developed constructs to increase the real applicability to *** a general introduction to musculoskeletal tissue engineering,this narrative review aims to offer an overview of methods,starting from classical techniques,such as gene expression analysis and histology,to less common methods,such as Raman spectroscopy,microcomputed tomography,and biosensors,that can be employed to assess the quality of constructs in terms of viability,morphology,or matrix deposition.A particular emphasis is given to standards and good practices(GXP),which can be applicable in different ***,a classification of the methods into destructive,noninvasive,or conservative based on the possible further development of a preimplant quality monitoring system is *** in musculoskeletal tissue engineering have not yet been used but have been proposed as a novel technology that can be exploited with numerous advantages,including minimal invasiveness,making them suitable for the development of preimplant quality control systems.

关键词： skeletal starting spite

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Association between Body Condition and Oxidative Status in Dogs

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Food and Nutrition Sciences 2013年第8期4卷 191-196页

作者： Anna Pasquini silvia Roberti Valentina Meucci elena luchetti Sergio Canello Gianandrea Guidetti Giulia Biagi Department of Veterinary Science University of Pisa Pisa Italy Sanypet Bagnoli di Sopra Padova Italy

Oxidative Stress (OS) is considered an underlying mechanism by which dysfunctional metabolism occurs in obese subjects but there are still very few studies in canine species. The purpose of this study was to evaluate simultaneously the effects of diet on body weight, oxidative and inflammatory status in a group of 12 adult dogs. The dogs were fed a maintenance diet, integrated with natural antioxidants, for a period of 6 months. At the beginning and the end of the trial, Body Condition Score (BCS) was evaluated and haematological (CBCs, Complete Blood Counts), oxidative parameters (d-ROMs, derivatives of Reactive Oxygen Metabolites;BAP, Biological Antioxidant Potential;Retinol;α-tocopherol) and inflammatory parameters (Fibrinogen;CRP, C Reactive Protein) were performed. Significant differences (p α-tocopherol, fibrinogen and CRP between the two periods of different diet. In this study, dogs showed an oxidative imbalance documented by the increase in d-ROMs and the reduction of BAP and retinol. Inflammatory parameters don’t change in relation to body weight like an alteration of the oxidative status could precede the onset of inflammation. The role of oxidative stress and of integration with antioxidants should be taken into special consideration in the dietary treatment in overweight dogs.

关键词： Canine Nutrition Overweight Oxidative Stress Antioxidants Inflammation

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