Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration

作     者：Melina Nisenbaum Gonzalo Hernn Sendra Gastón Alfredo Cerd Gilbert Marcelo Scagliola Jorge Froiln Gonzlez Silvia Elena Murialdo 

作者机构：National University of Mar del PlataFaculty of EngineeringDepartment of ChemistryBiochemical Engineering Group.Juan B.Justo 4302(7600)Mar del PlataArgentina National University of Mar del PlataFaculty of EngineeringLaser Laboratory7600Mar del PlataArgentina and Optical Research CenterCONICETLa Plata-CIC.PO Box No.3 1897GonnetLa PlataArgentina OSSE.Obras Sanitarias Mar del Plata-Batn S.E.Quality ManagementWater Laboratory.French 67377600Mar del PlataArgentina 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2013年第25卷第3期

页      面：613-625页

核心收录：

学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by15/G305ING311/11 and 1535/10CIC 

主　　题：dynamic laser speckle microorganisms biodegradation chemotaxis hydrocarbons 

摘      要：We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader,Pseudomonas strain *** biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with *** *** strain was able to degrade n-hexadecane,1-undecene,1-nonene,1-decene,1-dodecene and *** grew in the presence of 1-octene,while this hydrocarbons is toxic to other hydrocarbons *** strain H was also chemotactic towards n-hexadecane,kerosene,1-undecene and *** results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled *** the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations,we demonstrate the use of the dynamic speckle laser method,which is simple and inexpensive,to confirm bacterial chemotaxis at low cell concentrations （less than 105 colony-forming unit per millilitre （CFU/mL）） when hydrocarbons are the attractants.