Quality control methods in musculoskeletal tissue engineering:from imaging to biosensors

作     者：Daniele Zuncheddu Elena Della Bella Andrea Schwab Dalila Petta Gaia Rocchitta Silvia Generelli Felix Kurth Annapaola Parrilli Sophie Verrier Julietta V.Rau Marco Fosca Margherita Maioli Pier Andrea Serra Mauro Alini Heinz Redl Sibylle Grad Valentina Basoli Daniele Zuncheddu;Elena Della Bella;Andrea Schwab;Dalila Petta;Gaia Rocchitta;Silvia Generelli;Felix Kurth;Annapaola Parrilli;Sophie Verrier;Julietta V.Rau;Marco Fosca;Margherita Maioli;Pier Andrea Serra;Mauro Alini;Heinz Redl;Sibylle Grad;Valentina Basoli

作者机构：AC Research Institute DavosClavadelerstrasse 87270 DavosSwitzerland Regenerative Medicine Technologies LabEnte Ospedaliero Cantonale(EOC)Via Tesserete 466900 LuganoSwitzerland Department of MedicalSurgical and Experimental SciencesUniversity of SassariViale San Pietro 43/b07100 SassariItaly Centre Suisse d'Electronique et de MicrotechniqueBahnhofstrasse 17302 LandquartSwitzerland Center for X-ray AnalyticsEmpa-Swiss Federal Laboratories for Materials Science and TechnologyUberlandstrasse 1298600 DubendorfSwitzerland lstituto di Struttura della MateriaConsiglio Nazionale delle Ricerche(ISM-CNR)Via del Fosso del Cavaliere100-00133 RomeItaly Sechenov First Moscow State Medical UniversityTrubetskaya 8build.2119991 MoscowRussian Federation Department of Biomedical SciencesUniversity of SassariViale San Pietro 43/b07100 SassariItaly Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in AUVA trauma research centerDonaueschingenstraBe 131200 ViennaAustria Austrian Cluster for Tissue RegenerationViennaAustria 

出 版 物：《Bone Research》 (骨研究（英文版）)

年 卷 期：2021年第9卷第4期

页      面：473-493页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：European Union Horizon 2020 framework program Graubünden Innovationsstiftung AO Foundation, AO Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, SNF, (CRSK-3_190410) AOSpine 

主　　题：skeletal starting spite 

摘      要：Tissue engineering is rapidly progressing toward clinical *** the musculoskeletal field,there has been an increasing necessity for bone and cartilage *** the promising translational potential of tissue engineering approaches,careful attention should be given to the quality of developed constructs to increase the real applicability to *** a general introduction to musculoskeletal tissue engineering,this narrative review aims to offer an overview of methods,starting from classical techniques,such as gene expression analysis and histology,to less common methods,such as Raman spectroscopy,microcomputed tomography,and biosensors,that can be employed to assess the quality of constructs in terms of viability,morphology,or matrix deposition.A particular emphasis is given to standards and good practices(GXP),which can be applicable in different ***,a classification of the methods into destructive,noninvasive,or conservative based on the possible further development of a preimplant quality monitoring system is *** in musculoskeletal tissue engineering have not yet been used but have been proposed as a novel technology that can be exploited with numerous advantages,including minimal invasiveness,making them suitable for the development of preimplant quality control systems.