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Enzyme-mediated proximity labeling for mapping molecular interactions

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Science China(Life Sciences) 2023年第11期66卷 2680-2683页

作者： Xinrui Fan Xinwen yan shuo Han State Key Laboratory of Molecular Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China

The interaction between biomolecules,such as protein-protein interactions,protein-nucleic acid interactions,and protein-metabolite interactions,plays a central role in regulating cellular function.Yet sensitive detection and unbiased analysis of these interactions with high spatial and temporal resolution in living cells and organisms in situ remain challenging.Thus,the development and application of novel tools for molecular interaction mapping will contribute significantly to our understanding of many key biological processes.

关键词： function. mapping proximity

Amoeba-inspired magnetic microgel assembly assisted by engineered dextran-binding protein for vaccination against lifethreatening systemic infection

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Nano Research 2023年第1期16卷 938-950页

作者： shuo Liu yan Zhao Linpei Guo Qilin Yu Key Laboratory of Molecular Microbiology and Technology Ministry of EducationDepartment of MicrobiologyCollege of Life SciencesNankai UniversityTianjin 300071China Research Center for Infectious Diseases Nankai UniversityTianjin 300350China College of Environmental Science and Engineering Tianjin Key Laboratory of Environmental Remediation and Pollution ControlNankai UniversityTianjin 300350China Department of Urology The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical UniversityWuxi 214002China

Vaccination is critical for population protection from pathogenic infections.However,its efficiency is frequently compromised by a failure of antigen retention and presentation.Herein,we designed a dextran-binding protein DexBP,which is composed of the carbohydrate-binding domains of Trichoderma reesei cellobiohydrolases Cel6A and Cel7A,together with the sequence of the fluorescent protein mCherry.DexBP was further prepared by engineered Escherichia coli cells and grafted to magnetic nanoparticles.The magnetic nanoparticles were integrated with a dextran/poly(vinyl alcohol)framework and a reactive oxygen species-responsive linker,obtaining magnetic polymeric microgels for carrying pathogen antigen.Similar to amoeba aggregation,the microgels self-assembled to form aggregates and further induced dendritic cell aggregation.This step-by-step assembly retained antigens at lymph nodes,promoted antigen presentation,stimulated humoral immunity,and protected the mice from lifethreatening systemic infections.This study developed a magnetic microgel-assembling platform for dynamically regulating immune response during protection of the body from dangerous infections.

关键词： magnetic nanoparticle engineered protein dextran microgel vaccination systemic infection

On Disjoint Cycles of the Same Length in Tournaments

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Acta Mathematicae Applicatae Sinica 2022年第2期38卷 271-281页

作者： YUN WANG JIN yan shuo ZHU School of Mathematics Shandong UniversityJinan 250100China

A tournament is an orientation of the complete graph.Tournaments form perhaps the most interesting class of digraphs and it has a great potential for application.Tournaments provide a model of the statistical technique called the method of paired comparisons and they have also been studied in connection with sociometric relations in small groups.In this paper,we investigate disjoint cycles of the same length in tournaments.In 2010,Lichiardopol conjectured that for given integers l≥3 and k≥1,any tournament with minimum out-degree at least(l-1)k-1 contains k disjoint l-cycles,where an l-cycle is a cycle of order l.Bang-Jensen et al.verified the conjecture for l=3 and Ma et al.proved that it also holds for l≥10.This paper provides a proof of the conjecture for the case of 9≥l≥4.

关键词： tournaments minimum out-degree disjoint cycles

Electrospun Fe_(0.64)Ni_(0.36)/MXene/CNFs nanofibrous membranes with multicomponent heterostructures as flexible electromagnetic wave absorbers

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Nano Research 2023年第2期16卷 3395-3407页

作者： shuo Zhang Zirui Jia yan Zhang Guanglei Wu Institute of Materials for Energy and Environment State Key Laboratory of Bio-fibers and Eco-textilesCollege of Materials Science and EngineeringQingdao UniversityQingdao 266071China College of Chemistry and Chemical Engineering Qingdao UniversityQingdao 266071China Weihai Innovation Institute Qingdao UniversityWeihai 264200China

Two-dimensional metal carbide or nitride materials(MXenes)are widely used in electromagnetic wave absorption because of their unique structure.Herein,a novel composite preparation strategy has been proposed to design dendritic nanofibers based on the electrostatic spinning methods.The multifunctional MXene nanosheets are used as the dendritic matrix,and magnetic nanoparticles are embedded in the nanosheets as magnetic loss units.Multidimensional nanocomposites have interlaced carbon fiber networks,large-scale magnetically coupled networks,and a lot of multi-heterojunction interface structures,which endow the composites with extraordinary conduction loss,magnetic loss,and polarization loss capabilities,respectively.The impedance matching and loss mechanisms of the composites are improved by optimizing the synergistic relationship between the components and building a suitable structure.The optimum reflection loss(RL)of−54.1 dB is achieved at 2.7 mm and a wide effective absorption bandwidth(EAB,RL below−10 dB)of 7.76 GHz is obtained at a small thickness of 2.1 mm for the nanocomposites.The distinctive microstructures of the nanofibrous membranes give rise to their flexibility,waterproof,and electromagnetic wave absorption performance and endow the nanofibrous membranes potential to be utilized as lightweight,efficient electromagnetic wave protective fabric in harsh environment.

关键词： MXene Fe_(0.64)Ni_(0.36) nanofibers electromagnetic wave absorption electrospinning

An AIE Star Polymer with Enhanced Co-Delivery of Drug and Gene for Synergistic Pest Control

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Chinese Journal of Chemistry 2023年第20期41卷 2671-2678页

作者： Bingcheng Zhou Qinhong Jiang Jie Li Shuo Yan Jie Shen Lianying Liu Meizhen Yin State Key Laboratory of Chemical Resource Engineering Beijing Lab of Biomedical MaterialsBeijing University of Chemical TechnologyBeijing100029 China Department of Plant Biosecurity and MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests College of Plant ProtectionChina Agricultural UniversityBeijing100193 China

Pesticides,as the most common means of pest managements,have caused a series of problems such as pest resistance and environmental pollution.Aggregation-induced emission(AIE)polymers exhibit great potential in biological applications because their fluorescent intensities significantly enhance in the aggregated state.In this paper,an AIE star polymer including a tetraphenyl ethylene(TPE)core and poly(2-(dimethylamino)ethyl methacrylate)arms is designed and further developed as a multi-functional nanocarrier for agricultural pest control.The nanocarrier shows high water solubility,good size stability and AIE imaging ability.Meanwhile,the twisted AIE core and positively charged polymer arms make the nanocarrier efficiently co-load dinotefuran(DIN)and dsRNA via hydrogen bonds and electrostatic interactions,respectively.The AIE star polymer displays low toxicity and high fluorescence traceability.As a result,the nanocarrier co-loading with DIN and dsRNA exhibits a highly synergistic insecticidal effect with higher pest mortality compared to the separate delivery of DIN and dsRNA.This study develops the AIE star polymer to improve the efficiency of co-delivery of drug and dsRNA and proposes a new strategy toward efficient and synergistic pest control.

关键词： Pest control Nano-delivery system Drug nanocarrier Functional polymers Aggregation-induced emission

Nanocarrier-delivered dsRNA suppresses wing development of green peach aphids

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Insect Science 2022年第3期29卷 669-682页

作者： Yun-Hui Zhang Zhong-Zheng Ma Hang Zhou Zi-Jian Chao Shuo Yan Jie Shen Department of Plant Biosecurity and MOA Key Lab of Pest Monitoring and Green Management College of Plant ProtectionChina Agricultural UniversityBeijingChina

RNA interference(RNAi)has developed rapidly as a potential“green”pest management strategy.At present,most studies have focused on the screening of aphid lethal genes,whereas only a few studies have been conducted on wing development,which is crucial for aphid migration and plant-virus dissemination.Here,the Myzus persicae genes vestigial(vg)and Ultrabithorax(Ubx)related to wing development,were cloned.These two genes were expressed in various tissues of 3rd-instar winged aphids.The mRNA level of vg was high in 3rd-instar nymphs,whereas the expression level of Ubx was high in adults.The nanocarrier-mediated delivery system delivered double-stranded RNAs for aphid RNAi using topical and root applications.The expression levels of vg and Ubx were downregulated by 44.0%and 36.5%,respectively,using the topical application.The simultaneous RNAi of the two target genes caused 63.3%and 32.2%wing aberration rates using topical and root applications,respectively.The current study provided a promising method for controlling aphid migration to alleviate the spread of insect transmitted plant diseases.

关键词： nanocarrier RNA interference Ultrabithorax vestigial wing development

A nano-delivery system expands the insecticidal target of thiamethoxam to include a devastating pest,the fall armyworm

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Insect Science 2023年第3期30卷 803-815页

作者： Qin-Hong Jiang Ting Li yan Liu Zi-Yi Zhou yang yang Ying Wei Mei-Zhen Yin Jie Shen Shuo Yan Department of Plant Biosecurity and MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests College of Plant ProtectionChina Agricultural UniversityBejingChina State Key Laboratory of Chemical Resource Engineering Bejing Lab of Biomedical MaterialsBejing University of Chemical TechnologyBeijingChina

Nano-delivery systems have been applied to deliver various synthetic/botanical pesticides to increase the efficiency of pesticide use and reduce the volumes of pesticides applied.Previous studies have supported the hypothesis that the nanocarriers can help expand the insecticidal target of pesticides to include non-target pests.However,the potential mechanism underlying this interesting phenomenon remains unclear.Herein,a widely applied star polycation(SPc)nanocarrier was synthesized to construct a thiamethoxam(TMX)nano-delivery system.The SPc-based delivery system could promote the translocation of exogenous substances across the membrane of Sf9 cells,increase the cytotoxicity of TMX against Sf9 cells by nearly 20%,and expand the insecticidal target of TMX to include Spodoptera frugiperda(the fall armyworm),with a 27.5%mortality increase at a concentration of 0.25 mg/mL.Moreover,the RNA-seq analysis demonstrated that the SPc could upregulate various transport-related genes,such as Rab,SORT1,CYTH,and PIKfive,for the enhanced cellular uptake of TMX.Furthermore,enhanced cell death in larvae treated with the TMX-SPc complex was observed through changes in the expression levels of death-related genes,such as Casp7,BIRC5,MSK1,and PGAM5.The SPc-based nano-delivery system improved the cellular uptake of TMX and expanded its insecticidal target by adjusting the expression levels of death-related genes.The current study mainly identified the transport and cell death genes related to nanocarrier-based insecticidal target expansion,which is beneficial for understanding the bioactivity enhancement of the nano-delivery system.

关键词： insecticides nanotechnology nanomaterials thiamethoxam

Neuroprotective mechanisms ofε-viniferin in a rotenone-induced cell model of Parkinson's disease:significance of SIRT3-mediated FOXO3 deacetylation

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Neural Regeneration Research 2020年第11期15卷 2143-2153页

作者： shuo Zhang yan Ma Juan Feng Department of Neurology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina Department of Ultrasound Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

Trans-(-)-ε-viniferin(ε-viniferin)has antioxidative and anti-inflammatory effects.It also has neuroprotective effects in Huntington's disease by activating the SIRT3/LKB1/AMPK signaling pathway;however,it remains unknown whetherε-viniferin also has a neuroprotective role in Parkinson's disease.A Parkinson's disease cell model was induced by exposing SH-SY5 Y cells to 3.0μM rotenone for 24 hours,and cells were then treated with 1.0μMε-viniferin for 24 hours.Treatment withε-viniferin upregulated SIRT3 expression,which promoted FOXO3 deacetylation and nuclear localization.ε-Viniferin also increased ATP production and decreased reactive oxygen species production.Furthermore,ε-viniferin treatment alleviated rotenone-induced mitochondrial depolarization and reduced cell apoptosis,and restored the expression of mitochondrial homeostasis-related proteins.However,when cells were transfected with SIRT3 or FOXO3 shRNA prior to rotenone andε-viniferin treatment,these changes were reversed.The results from the present study indicate thatε-viniferin enhances SIRT3-mediated FOXO3 deacetylation,reduces oxidative stress,and maintains mitochondrial homeostasis,thus inhibiting rotenone-induced cell apoptosis.ε-Viniferin may therefore be a promising treatment strategy for Parkinson's disease.

关键词： deacetylation FOXO3 mitochondrial homeostasis mitophagy oxidative stress Parkinson's disease SIRT3 ε-viniferin

Study on the morphological regulation mechanism of hollow silica microsphere prepared via emulsion droplet template

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Chinese Chemical Letters 2023年第4期34卷 575-579页

作者： Chu Zhao Zhiqing Ge Zhuoni Jiang Shuo Yan Jingjing Shu Mozhen Wang Xuewu Ge CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of ChinaHefei 230026China School of Materials Science and Engineering Southwest University of Science and TechnologyMianyang 621010China

The morphology regulation of hollow silica microspheres is significant for their properties and applications. In this paper, hollow silica microspheres were formed through the hydrolysis and condensation reaction of tetraethyl orthosilicate(TEOS) at the interface of the emulsion droplet templates composed of liquid paraffin and TEOS, followed by dissolving paraffin with ethanol. The effects of various factors including the emulsifier structure and content, TEOS content, catalyst type, and the ethanol content in the continuous water phase on the particle size, shell thickness and morphology of the prepared hollow silica microspheres were studied in detail. The results show that the diffusion and contact of TEOS and water molecules as well as the hydrolysis condensation reaction of TEOS at the oil-water interface are two critical processes for the synthesis and morphological regulation of hollow silica microspheres. Cationic emulsifier with a hydrophobic chain of appropriate length is the prerequisite for the successful synthesis of hollow silica microspheres. The ethanol content in water phase is the dominant factor to determine the average diameter of hollow microspheres, which can vary from 96 nm to 660 nm with the increase of the volume ratio of alcohol-water from 0 to 0.7. The silica wall thickness varies with the content and the hydrophobic chain length of the emulsifier, TEOS content, and the activity of the catalyst. The component of the soft template will affect the morphology of the silica wall. When the liquid paraffin is replaced by cyclohexane, hollow microspheres with fibrous mesoporous silica wall are fabricated. This work not only enriches the basic theory of interfacial polymerization in the emulsion system, but also provides ideas and methods for expanding the morphology and application of hollow silica microspheres.

关键词： Hollow silica microspheres Emulsion template Morphology regulation mechanism Interfacial polymerization