Amoeba-inspired magnetic microgel assembly assisted by engineered dextran-binding protein for vaccination against lifethreatening systemic infection

作     者：Shuo Liu Yan Zhao Linpei Guo Qilin Yu Shuo Liu;Yan Zhao;Linpei Guo;Qilin Yu

作者机构：Key Laboratory of Molecular Microbiology and TechnologyMinistry of EducationDepartment of MicrobiologyCollege of Life SciencesNankai UniversityTianjin 300071China Research Center for Infectious DiseasesNankai UniversityTianjin 300350China College of Environmental Science and EngineeringTianjin Key Laboratory of Environmental Remediation and Pollution ControlNankai UniversityTianjin 300350China Department of UrologyThe Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical UniversityWuxi 214002China 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2023年第16卷第1期

页      面：938-950页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 070303[理学-有机化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：This work was supported by the National Natural Science Foundation of China(Nos.3217010793 and 31870139) Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(No.TSBICIP-KJGG-006) the Natural Science Foundation of Tianjin(No.19JCZDJC33800) the Fundamental Research Funds for the Central Universities. 

主　　题：magnetic nanoparticle engineered protein dextran microgel vaccination systemic infection 

摘      要：Vaccination is critical for population protection from pathogenic infections.However,its efficiency is frequently compromised by a failure of antigen retention and presentation.Herein,we designed a dextran-binding protein DexBP,which is composed of the carbohydrate-binding domains of Trichoderma reesei cellobiohydrolases Cel6A and Cel7A,together with the sequence of the fluorescent protein mCherry.DexBP was further prepared by engineered Escherichia coli cells and grafted to magnetic nanoparticles.The magnetic nanoparticles were integrated with a dextran/poly(vinyl alcohol)framework and a reactive oxygen species-responsive linker,obtaining magnetic polymeric microgels for carrying pathogen antigen.Similar to amoeba aggregation,the microgels self-assembled to form aggregates and further induced dendritic cell aggregation.This step-by-step assembly retained antigens at lymph nodes,promoted antigen presentation,stimulated humoral immunity,and protected the mice from lifethreatening systemic infections.This study developed a magnetic microgel-assembling platform for dynamically regulating immune response during protection of the body from dangerous infections.