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中华临床感染病杂志 2024年第4期17卷 309-311and320页

作者：马严刚魏书俊许静日照市中医医院运动医学科日照日照市人民医院老年医学科日照

停乳链球菌停乳亚种主要见于动物体内, 人感染少见, 免疫功能低下和接触海鲜/牲畜者为易感人群, 一般预后良好。本文报道1例停乳链球菌停乳亚种致大腿脓肿患者, 经治疗后痊愈, 供临床参考。

关键词：链球菌停乳链球菌停乳亚种脓肿

Genome-wide characterization of microsatellites and development of polymorphic markers shared between two weevils of Eucryptorrhynchus(Coleoptera:Curculionidae)

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Zoological Systematics 2021年第4期46卷 273-280页

作者： Yujie Zhang wei Song Jincui Chen Lijun Cao Junbao Wen shujun wei Beijing Key Laboratory for Forest Pest Control Beijing Forestry UniversityBeijing 100083China Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

The weevils Eucryptorrhynchus brandti(Harold)and ***(Motschulsky)are two pests of the tree of heaven,Ailanthus *** usually co-occur on their host ***,these two species have a niche separation with different eggs-laying locations and larvae-feeding parts on the ***,we characterized the distribution of microsatellites in the genome and developed genome-wide microsatellite markers shared between these two weevils.A total of 12,524 and 11,030 potential microsatellites were identified for *** and ***,*** with AT,TA,AC,TG,CA repeat motifs were most common for both *** designed primers universal for two weevils and developed 14 loci with high amplification efficacy and *** validated these microsatellite markers in three populations of each *** ***,the observed and expected heterozygosity for the genotyped loci ranged from 0.17-0.24 and 0.44-0.54;in ***,the above values ranged from 0.06-0.18 and *** on the developed microsatellite markers,we found that populations of both weevils have a clear pattern of genetic *** study provides valuable genetic markers for ecological,genetic,and evolutionary study of these two weevils.

关键词： Microsatellite primer population genetics

Mitochondrial gene rearrangement within genus Gasteruption(Hymenoptera: Evanioidea: Gasteruptiidae)

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Zoological Systematics 2016年第2期41卷 133-139页

作者： shujun wei Lijun Cao Qiuling Wu Chaodong Zhu Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry Sciences College of Agronomy and Plant Protection Qingdao Agricultural University Key Laboratory of Zoological Systematics and Evolution (CAS) Institute of Zoology Chinese Academy of Sciences

The complete mitochondrial genome of the Gasteruption parvicollarium Enderlein （GenBank accession number： KR270643） was sequenced in the study. Totally 17 009 bp sequence was determined with an A＋T content of 83.81%, including full set of typical animal mitochondrial genes. Two protein-coding and 10 tRNA genes as well as the A＋T-rich region were rearranged compared with the putative ancestral arrangement of insects. Most of the rearranged genes were located in the ancestral region of trnl-trnQ-trnM-nad2-trn W-trnC-trn Y- coxl-trnL2. The other rearranged genes are trnN and trnS1 located in the tRNA cluster trnA- trnR-trnN-trnSl-trnE-trnF and trnS2 located between cob and nadl. Remote inversion is dominant rearrangement event in G. parvicollarium mitochondrial genome, involving two protein-coding and 8 tRNA genes. Compared with the other mitochondrial genome reported in the same genus of Gasteruption, the inverted trnN was translocated to the tRNA cluster between cox1 and nad2 in G. parvicollarium. This is the first report of mitochondrial gene rearrangement occurred within genus of Hymenoptera. Our study points to a recently occurred gene rearrangement event in the Gasteruption species.

关键词： Apocrita wasp mitochondrial genome , gene rearrangement phylogeny

大跨铝合金天桥人致振动舒适度评价及TMD减振控制

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广东土木与建筑 2024年第9期31卷 80-84页

作者：罗沐鑫吴子儒张潭潭韦树军徐其功广东省建科建筑设计院有限公司广州510010

为了减轻大跨铝合金天桥在人群荷载作用下的振动舒适度问题,针对大跨铝合金天桥安装调谐质量阻尼器(TMD)前后的振动特性进行研究。根据某两跨连续铝合金天桥进行有限元建模,分析结构自振特性,选择德国EN03人行桥设计指南,基于该规范下... 详细信息

为了减轻大跨铝合金天桥在人群荷载作用下的振动舒适度问题,针对大跨铝合金天桥安装调谐质量阻尼器(TMD)前后的振动特性进行研究。根据某两跨连续铝合金天桥进行有限元建模,分析结构自振特性,选择德国EN03人行桥设计指南,基于该规范下的人群荷载模式,选取5种交通级别,对比不同交通级别下天桥振动峰值加速度,获得天桥人致振动舒适度结果。并在天桥第一跨跨中和第二跨跨中分别安装一个TMD进行减振控制,对比设置TMD减振器前后天桥人致振动模型峰值加速度结果差异。结果表明:TMD可有效降低天桥结构竖向振动响应,根据选取的天桥振动位移加速度观察点结果可知,竖向减振率达到89.3%,对于不满足舒适度要求的大跨铝合金天桥,可布置适当数量的TMD阻尼器,且分布于合理位置有利于减轻天桥的振动,进而提高人行天桥的舒适性。

关键词：动力特性人致振动舒适度评价 TMD减振

Genome-wide developed microsatellite markers for the melon thrips Thrips palmi Karny(Thysanoptera:Thripidae)

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Zoological Systematics 2019年第2期44卷 100-110页

作者： Yongfu Gao Yajun Gong Ling Ma Lijun Cao Jincui Chen Min Chen shujun wei Beijing Key Laboratory for Forest Pest Control College of Forestry Beijing Forestry University Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry Sciences

The melon thrips, Thrips palmi Karny, is an economically important pest on many vegetables from Solanaceae and Cucurbitaceae. In this study, we developed novel microsatellite markers for Thrips palmi. First, we obtained randomly sequenced regions from the genome using next-generation sequencing and assembled 356 Mb genomic sequences. In total, 155,789 microsatellites were identified from the genomic sequences, of which, 64.02% were loci of dinucleotide repeat. Sixty primer pairs were initially validated in seven individuals. Thirty-five polymorphic markers were retained according to the amplification efficiency and *** 35 microsatellite loci were then assessed in 96 individuals in four geographical populations collected from China. The allele numbers ranged from 2 to 19 with an average value of 6.9 per locus, while the polymorphism information content ranged from 0.2219 to *** observed and expected heterozygosity varied from 0.5252 to 0.5367 and 0.5934 to 0.6148,respectively. Genetic structure was found between populations from southern and northern China. These novel microsatellite markers developed in our study provide abundant molecular markers for the genetic study of T. palmi.

关键词： Microsatellite genetic marker genome population genetic structure

Bronchoalveolar lavage fluid assessment facilitates precision medicine for lung cancer

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Cancer Biology & Medicine 2024年第3期21卷 230-251页

作者： Hantao Zhang Dan Deng shujun Li Jing Ren wei Huang Dan Liu weiya Wang Department of Pathology West China HospitalSichuan UniversityChengdu 610000China West China Biobank West China HospitalSichuan UniversityChengdu 610000China Department of Respiratory and Critical Care Medicine Clinical Research Center for Respiratory DiseaseWest China HospitalSichuan UniversityChengdu 610000China

Lung cancer is the most common and fatal malignant disease worldwide and has the highest mortality rate among tumor-related causes of *** diagnosis and precision medicine can significantly improve the survival rate and prognosis of lung cancer *** present,the clinical diagnosis of lung cancer is challenging due to a lack of effective non-invasive detection methods and biomarkers,and treatment is primarily hindered by drug resistance and high tumor *** biopsy is a method for detecting circulating biomarkers in the blood and other body fluids containing genetic information from primary tumor *** lavage fluid(BALF)is a potential liquid biopsy medium that is rich in a variety of bioactive substances and cell *** contains information on the key characteristics of tumors,including the tumor subtype,gene mutation type,and tumor environment,thus BALF may be used as a diagnostic supplement to lung *** this review,the current research on BALF in the diagnosis,treatment,and prognosis of lung cancer is *** advantages and disadvantages of different components of BALF,including cells,cell-free DNA,extracellular vesicles,and micro RNA are *** particular,the great potential of extracellular vesicles in precision diagnosis and detection of drug-resistant for lung cancer is *** addition,the performance of liquid biopsies with different body fluid sources in lung cancer detection are compared to facilitate more selective studies involving BALF,thereby promoting the application of BALF for precision medicine in lung cancer patients in the future.

关键词： Lung cancer bronchoalveolar lavage fluid extracellular vesicles liquid biopsy precision medicine

“一国两制”制度体系的显著优势及其完善和发展——以“一国两制”在香港特区的实践为视角

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山东师范大学学报（社会科学版） 2020年第4期65卷 88-96页

作者：魏淑君中国浦东干部学院教学研究部上海201204

从香港回归之日起,宪法和香港基本法共同构成香港特别行政区的宪制基础,是"一国两制"方针法律化的集中体现,也是"一国两制"制度体系的核心。"一国两制"制度体系的显著优势,是"一国两制"伟大构想从提出到付诸实践的经验升华和成就凝结。... 详细信息

从香港回归之日起,宪法和香港基本法共同构成香港特别行政区的宪制基础,是"一国两制"方针法律化的集中体现,也是"一国两制"制度体系的核心。"一国两制"制度体系的显著优势,是"一国两制"伟大构想从提出到付诸实践的经验升华和成就凝结。随着"一国两制"的深入实践,也会遇到一些新情况新问题,会面临着前所未有的挑战,为处理和应对这些新问题,维护特区长期繁荣稳定,确保"一国两制"行稳致远,"一国两制"制度体系也在不断完善和发展。

关键词：宪法基本法 “一国两制”制度体系香港特区

Research and development of the longitudinal feedback system digital signal processing electronics in BEPCII

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Radiation Detection Technology and Methods 2021年第2期5卷 213-219页

作者： Di Yin Junhui Yue Huizhou Ma Jianshe Cao shujun wei Cunbiao Hao Yanfeng Sui Jun He Jingxia Zhao Zhi Liu Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049People’s Republic of China 2 Accelerator Technology and Key Laboratory of Academy of Physics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049People’s Republic of China University of Chinese Academy Sciences Beijing 100049People’s Republic of China

Background The current strategy to achieve high luminosity for high energy physics experiments is to store huge currents distributed in many bunches in circular machines such as BEPCII,so the longitudinal coupled bunch instabilities of the beam are *** Therefore,a longitudinal feedback system is needed to suppress longitudinal ***,the beam energy spread will increase;the luminosity and the beam lifetime will decrease under a certain beam *** For filter design of the feedback system,the time domain least square fitting(TDLSF)method is compared to the selective wave filter method;digital signal processing electronics based on commercial board have been developed and tested on *** The final result shows that the self-developed longitudinal feedback system can suppress the longitudinal beam oscillation at 750 mA beam current and meet the requirements of BEPCII;the restrain depth to the sidebands reaches to 40.8 dB.

关键词： Longitudinal bunch instability BEPCII Feedback system

Conserved profiles of digestion by double restriction endonucleases in insect genomes facilitate the design of dd RAD

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Zoological Systematics 2018年第4期43卷 341-355页

作者： Bingyan Li Qiao Gao Lijun Cao Ary Anthony Hoffmann Qiong Yang Jiaying Zhu shujun wei Key Laboratory of Forest Disaster Warning and Control of Yunnan Province College of Forestry Southwest Forestry University Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry Sciences School of BioSciences Bio21 Institute The University of Melbourne

Double-digested Restriction Site Associated DNA Sequencing（ddRAD） through next-generation sequencing（NGS） generates large numbers of loci for characterizing genomewide variation among multiple samples using next-generation sequencing. Different combinations of restriction endonucleases（REs） may produce varying size distributions of digested fragments, which affect the number of genotyped loci. Understanding digestion profiles across different species will help in selecting REs for digestion in a particular organism. In this study, we use of genome sequences to compare the in silico digestion profile of 26 combinations of REs in 131 insect species with two simulation programs. The number of digested fragments in the 300-450 bp range increases linearly with the size of the genome. Different species and insect orders showed similar profiles when digested by different combinations of REs in silico, indicating the conservation of digestion by double enzymes in insect genomes. Combinations with Nla III or TaqαI usually produced higher number of fragments in the range 300-450 bp, while combinations with EcoRI or MluCI produced fewer fragments. The proportion of fragments with the same overhangs at the two ends of digested DNA was higher than those with different overhangs. The two four-base enzyme pairs produced more fragments in the 300-450 bp range than pairs of four-base ＋ six-base enzymes. Experimental digestion of three species from Hymenoptera, Lepidoptera and Thysanoptera showed profiles congruent with in silico expectations. Our results shed light on understanding the digestion profiles of insect genomes and provide guidance on selecting REs for ddRAD projects.

关键词： Double-digested RADseq in silico simulation insect genome optimal doubledigestion combination

“同一性”下的“多样性”:和谐社会法律文化的构思

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理论前沿 2007年第18期 22-23页

作者：魏淑君中国浦东干部学院教学研究部

各国法律文化既体现着鲜明的同一性,又体现着异常鲜明的多样性特征。我国要建立的和谐社会法律文化以和谐为精神内核、以公平正义为核心价值观,是法律文化"多样性"和"同一性"的集中体现。

关键词：法律文化同一性多样性和谐社会