Genome-wide developed microsatellite markers for the melon thrips Thrips palmi Karny(Thysanoptera:Thripidae)

作     者：Yongfu Gao Yajun Gong Ling Ma Lijun Cao Jincui Chen Min Chen Shujun Wei 

作者机构：Beijing Key Laboratory for Forest Pest Control College of Forestry Beijing Forestry University Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry Sciences 

出 版 物：《Zoological Systematics》 (动物分类学报（英文版）)

年 卷 期：2019年第44卷第2期

页      面：100-110页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治] 

基　　金：provided jointly by Promotion and Innovation of Beijing Academy of Agriculture and Forestry Sciences(KJCX20180113) the Natural Science Foundation of Beijing Municipality(6184037) Beijing Key Laboratory of Environmentally Friendly Pest Management on Northern Fruits(BZ0432) 

主　　题：Microsatellite genetic marker genome population genetic structure 

摘      要：The melon thrips, Thrips palmi Karny, is an economically important pest on many vegetables from Solanaceae and Cucurbitaceae. In this study, we developed novel microsatellite markers for Thrips palmi. First, we obtained randomly sequenced regions from the genome using next-generation sequencing and assembled 356 Mb genomic sequences. In total, 155,789 microsatellites were identified from the genomic sequences, of which, 64.02% were loci of dinucleotide repeat. Sixty primer pairs were initially validated in seven individuals. Thirty-five polymorphic markers were retained according to the amplification efficiency and *** 35 microsatellite loci were then assessed in 96 individuals in four geographical populations collected from China. The allele numbers ranged from 2 to 19 with an average value of 6.9 per locus, while the polymorphism information content ranged from 0.2219 to *** observed and expected heterozygosity varied from 0.5252 to 0.5367 and 0.5934 to 0.6148,respectively. Genetic structure was found between populations from southern and northern China. These novel microsatellite markers developed in our study provide abundant molecular markers for the genetic study of T. palmi.