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Recurrent factors and expression of GLUT-1,PI3K and p-Akt in adenoid cystic carcinomas of the head and neck——clinicopathological features and biomarkers study of adenoid cystic carcinoma

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Recurrent factors and expression of GLUT-1,PI3K and p-Akt in...

Effects of different diets on intestinal microbiota and nonalcoholic fatty liver disease development

2012年浙江省耳鼻咽喉科学学术年会

作者： shui-Hong Zhou Jin Fang Hong-Tian Yao jian-Feng he 浙江大学医学院附属第一医院耳鼻咽喉科

The purpose of this study was to explore factors associated with recurrence of adenoid cystic carcinomas (ACCs).We examined recurrence values of clinicopathologic variables,GLUT-l,p-Akt,and PI3K expression in 42 patients with *** the 42 patients,seventeen developed recurrence after initial *** positive rate of GLUT-1,PI3K,and p-Akt protein in ACC was 38.1%,38.1%,and 50.0%,respectively.T Expression of GLUT-1 or p-Akt or PI3K protein in ACC was higher than that in inflammatory lesions or benign *** study has demonstrated that T stage,a positive resection margin,perineural invasion,surgery without postoperative radiotherapy,and expression of GLUT-1,PI3K,and p-Akt were factors predictive of recurrence by univariate *** multivariable analysis,perineural invasion,resection margin positive and p-Akt were significant predictors of *** surgery is very important for recurrence of *** of GLUT-1, PI3K,and p-Akt may play a role in development recurrence of ACC.

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World Journal of Gastroenterology 2016年第32期22卷 7353-7364页

作者： jian-Ping Liu Wen-Li Zou shui-Jiao Chen Hong-Yun Wei Ya-Ni Yin Yi-You Zou Fang-Gen Lu Department of Gastroenterology Second Xiangya Hospital of Central South University Department of Nephrology Zhejiang Provincial People’s Hospital Department of Gastroenterology Xiangya Hospital of Central South University

AIM To study the effects of different diets on intestinal microbiota and nonalcoholic fatty liver disease(NAFLD) development at the same caloric *** Thirty male Sprague-Dawley rats were randomized into five groups(six rats each). The control diet(CON) group and free high-fat diet(FFAT) group were allowed ad libitum access to a normal chow diet and a highfat diet, respectively. The restrictive high-fat diet(RFAT) group, restrictive high-sugar diet(RSUG) group, and high-protein diet(PRO) group were fed a highfat diet, a high-sugar diet, and a high-protein diet, respectively, in an isocaloric way. All rats were killed at 12 wk. Body weight, visceral fat index(visceral fat/body weight), liver index(liver/body weight), insulin resistance, portal lipopolysaccharide(LPS), serum alanine aminotransferase(ALT), serum aspartate aminotransferase(AST), and liver triglycerides were measured. The intestinal microbiota in the different groups of rats was sequenced using high-throughput sequencing *** The FFAT group had higher body weight, visceral fat index, liver index, peripheral insulin resistance, portal LPS, serum ALT, serum AST, and liver triglycerides compared with all other groups(P he same calories, the RFAT and RSUG groups demonstrated increased body weight, visceral fat index, peripheral insulin resistance and liver triglycerides compared with the PRO group(P he RFAT group also showed increased portal LPS compared with the PRO group(P he sequencing data revealed that the intestinal microbiota structures of the CON, FFAT, RSUG and PRO groups were roughly separated away from each other. Taxon-based analysis showed that, compared with the CON group, the FFAT group had an increased abundance of Firmicutes, Roseburia and Oscillospira bacteria, a higher ratio of Firmicutes to Bacteroidetes, and a decreased abundance of Bacteroidetes, Bacteroides and Parabacteroi

关键词： Nonalcoholic fatty liver disease High-fat diet Restrictive high-fat diet Restrictive high-sugar diet High-protein diet Intestinal microbiota

hepatocyte isolation from resected benign tissues: Results of a 5-year experience

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World Journal of Gastroenterology 2016年第36期22卷 8178-8186页

作者： Fan-Ying Meng Li Liu Jun Liu Chun-You Li jian-Ping Wang Feng-Hui Yang Zhi-shui Chen Ping Zhou Division of hepato-Biliary-Pancreatic Surgery Institute of Organ Transplantation Shandong Provincial Hospital affiliated to Shandong University Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

AIM To analyze retrospectively a 5-year experience of human hepatocyte isolation from resected liver tissues with benign *** We established a method of modified four-step retrograde perfusion to isolate primary human hepatocytes. Samples were collected from the resected livers of patients with intrahepatic duct calculi(n = 7) and liver hemangioma(n = 17). Only the samples weighing ≥ 15 g were considered suitable for hepatocyte isolation. By using the standard trypan blue exclusion technique, hepatocyte viability and yield were immediately determined after *** Twenty-four liver specimens, weighing 15-42 g, were immediately taken from the margin of the removed samples and transferred to the laboratory for hepatocyte isolation. Warm ischemia time was 5-35 min and cold ischemia time was 15-45 min. For the 7 samples of intrahepatic duct calculi, the method resulted in a hepatocyte yield of 3.49 ± 2.31 × 10~6 hepatocytes/g liver, with 76.4% ± 10.7% viability. The 17 samples of liver hemangioma had significantly higher yield of cells(5.4 ± 1.71 × 10~6 cells/g vs 3.49 ± 2.31 × 10~6 cells/g, P he samples of intrahepatic duct calculi. However, there seems to be no clear difference in cell viability(80.3% ± 9.67% vs 76.4% ± 10.7%, P > 0.05). We obtained a cell yield of 5.31 ± 1.87 × 10~6 hepatocytes/g liver when the samples weighed > 20 g. However, for the tissues weighing ≤ 20 g, a reduction in yield was found(3.08 ± 1.86 × 10~6 cells/g vs 5.31 ± 1.87 × 10~6 cells/g, P hepatocyte isolation. Our study represents the largest number of primary human hepatocytes isolated from resected specimens from patients with benign liver disease. We evaluated the effect of donor liver characteristics on cell isolation, and we found that samples of liver hemangioma can provide better results than intrahepatic duct calculi, in terms of cell yield. Furthermore, the size of the

关键词： Human hepatocyte Primary hepatocyte Cell isolation Benign liver disease hepatocyte isolation

Protective Effect of Ginsenoside Rd on Lipopolysaccharide-Induced Acute Lung Injury through its Anti-Inflammatory and Anti-Oxidative Activity

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World Journal of Traditional Chinese Medicine 2021年第3期7卷 383-390页

作者： Jv Chen Wan-Xian Fang Shao-Jin Li shui-Xiu Xiao Hai-jian Li Yong-Li Situ Department of Pharmacy Affiliated Hospital of Guangdong Medical UniversityZhanjiang Guangzhou Darui Biotechnology Co. Ltd.Guangzhou Obstetrics and Gynecology Shenzhen Longhua District Central Hospital Medical Laboratory Shenzhen Longhua District Central HospitalShenzhen Department of Clinical Laboratory Medicine Guangdong Second Provincial General HospitalGuangzhou College of pharmacy Jinan UniversityGuangzhouChina

Background:Inflammation and oxidation stress are key factors in the mechanism of acute lung injury(ALI).Therefore,suppression of the inflammatory response and oxidative stress could be a potential strategy to treat lipopolysaccharide(LPS)-induced *** Rd(Rd),a natural Ginseng extract,alleviates inflammation and oxidative stress in several diseases such as Alzheimer’s disease and cerebral ischemia,but its effect on ALI is still *** and Objectives:To explore the protective effect of Rd on LPS-induced ALI and explored associated *** and Methods:Mice were divided into five groups:A sham-operated group,a LPS-induced ALI group,and three LPS groups pretreated with Rd doses of 20,40,and 80 mg/kg,*** pathological changes of lung,collagen deposition,pulmonary edema,inflammatory cytokine,oxidative stress and the expression levels of TLR4 and NF-κB were ***:The oral administration of Rd dose dependently attenuated histopathologic changes in the lung,lung edema,pulmonary collagen deposition,protein concentration in bronchoalveolar lavage fluid(BALF),myeloperoxidase(MPO)activity,and inflammatory cell *** addition,Rd suppressed the LPS-induced inflammatory cytokines tumor necrosis factor-α,interleukin(IL)-6,and IL-1βin *** productions of oxidative stress-related enzymes(catalase,superoxide dismutase,and glutathione peroxidase)in lung tissue were significantly upregulated by Rd ***,malondialdehyde and pulmonary MPO activity was reduced in the Rd-pretreated groups when compared with LPS-induced ALI *** treatment also dose dependently suppressed LPS-induced NF-κB activation and TLR4 ***:Overall,these findings provide evidence that Rd pretreatment inhibits LPS-induced ALI through anti-inflammatory and antioxidative actions,suggesting that it could be a promising protective drug for LPS-induced ALI.

关键词： Acute lung injury ginsenoside Rd inflammatory oxidative stress

Soil C and N Pools in Chinese Fir and Evergreen Broadleaf Forests and their Changes with Slash Burning in Mid-Subtropical China

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Pedosphere 2006年第1期16卷 56-63页

作者： GUO jian-Fen YANG Yu-Sheng CheN Guang-shui XIE Jin-Sheng LIN Peng College of Life Science Xiamen University Xiamen 361005 （China） College of Geography Science Fujian Normal University Fuzhou 350007 （China） College of Forestry Fujian Agriculture and Forestry University Fuzhou 350002 （China）

Soil organic carbon （C） and total nitrogen （N） pools of a Chinese fir （Cunninghamia lanceolata （Lamb.） Hook.）（CF） forest, and an evergreen broadleaf （EB） forest located in mid-subtropical, southeastern China, were compared before clearcutting, with the effect of slash burning on organic C and total N in the top 10 cm of soil before and after burning also being evaluated. Prior to clearcutting CF forest had significantly lower （P 〈0.05） organic C and total N in the soil （0-100 cm） compared to EB forest with approximately 60% of the C and N at the two forest sites stored at the 0 to 40 cm soil. In post-burn samples of the 0-10 cm depth at 5 days, 1 year, and 5 years for CF and EB forests, significantly lower levels （P 〈0.05） of organic C and total N than those in the pre-burn samples were observed. Compared to the pre-burn levels, at post-burn year 5, surface soil organic C storage was only 85% in CF forest and 72% in EB forest, while total N storage was 77% for CF forest and 73% for EB forest. Slash burning caused marked long-term changes in surface soil C and N in the two forest types.

关键词： Chinese fir evergreen broadleaf forest slash burning soil carbon soil nitrogen

Recurrent factors and expression of GLUT-1,PI3K and p-Akt in adenoid cystic carcinomas of the head and neck

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Recurrent factors and expression of GLUT-1,PI3K and p-Akt in...

Exploring the Mechanism of Tripterygium wilfordii Against Cancer Using Network Pharmacology and Molecular Docking

全国耳鼻咽喉头颈外科中青年学术会议

作者： shui-Hong Zhou Jin Fang Hong-Tian Yao jian-Feng he The First Affiliated Hospital College of MedicineZhejiang University

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World Journal of Traditional Chinese Medicine 2022年第3期8卷 417-425页

作者： shui-Xiu Xiao Shao-Jin Li Wan-Xian Fang Jv Chen Hai-jian Li Yong-Li Situ Obstetrics and Gynecology Shenzhen Longhua District Central HospitalShenzhen 518110GuangdongChina Medical Laboratory Shenzhen Longhua District Central HospitalShenzhen 518110GuangdongChina Research Department Guangzhou Darui Biotechnology Co.Ltd.Guangzhou 510670GuangdongChina Department of Pharmacy Affiliated Hospital of Guangdong Medical UniversityZhanjiang 524023GuangdongChina Department of Clinical Laboratory Medicine Guangdong Second Provincial Central HospitalGuangzhou 510317GuangdongChina College of Pharmacy Jinan UniversityGuangzhou 510632GuangdongChina

Background: The root of Tripterygium wilfordii(Tripterygii radix), a natural powerful traditional Chinese medicine(TCM) for various diseases treatment, has been used for centuries in the Asian countries as anti-rheumatoid arthritis(RA) agent, antioxidant agent, and anti-inflammatory agent. Its combination with other herbs in treating RA has been explored. The anti-RA effect of T. wilfordii for cancer treatment has been supported by some evidence. Aims and Objectives: To investigate the anticancer mechanism of T. wilfordii, bioinformatics databases were used to identify its active ingredients. Materials and Methods: Target proteins associated with cancer were determined using a network pharmacology analysis platform, and 25 key active compounds and 55 key targets of T. wilfordii were identified in our study. A common potential mechanism of T. wilfordii involvement in cancer was disclosed by in-depth network analysis of diseases, functions, and ***, the analysis results of the TCM-disease target protein interaction network revealed 5 potential targets;subsequently, a total of 30targets(these 5 targets, as well as 25 previously identified compounds) were subjected to molecular docking. Results: Our results showed that the therapeutic effect of T. wilfordii in cancer is characterized by multiple components, targets, and pathways. The regulation of signaling pathways such as Kaposi sarcoma-associated herpes virus infection, colorectal cancer, small-cell lung cancer, and prostate cancer may be the important pharmacodynamic basis of anticancer therapy. Conclusion: Triptonoditerpenic acid inhibited proliferation and induced apoptosis in SW480 cells. The mechanism may be related to the downregulation of Bcl-2 expression, upregulation of Bax mRNA expression, and expression inhibition of PTGS2.

关键词： Tripterygium wilfordii cancer network pharmacology molecular docking

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碳基三维自支撑超级电容器电极材料研究进展

电化学 2015年第6期21卷 518-533页

作者：何水剑陈卫中国科学院长春应用化学研究所电分析化学国家重点实验室吉林长春130022 中国科学院大学北京100039

自支撑电极材料在超级电容器中有着广泛的应用.碳材料具有结构多样、来源丰富、价格低廉以及性能稳定等优点,是构建三维自支撑电极材料的首选基底材料.本文结合作者课题组的研究工作,从"由上而下"和"由下而上"两个方面,概述了设计、制... 详细信息

自支撑电极材料在超级电容器中有着广泛的应用.碳材料具有结构多样、来源丰富、价格低廉以及性能稳定等优点,是构建三维自支撑电极材料的首选基底材料.本文结合作者课题组的研究工作,从"由上而下"和"由下而上"两个方面,概述了设计、制备三维自支撑电极材料的常用方法及材料的电容性能,希望对开发利用天然可再生资源,制备高性能的自支撑电极材料及其在超级电容器材料中的应用有所帮助.

关键词：碳材料自支撑基底电极材料超级电容器

Analysis of the ratio of mitchondrial DNA with A1555G mutant to wild type in deaf patients of Fujian province in China by a new method and its relationship with the severity of hearing loss

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Chinese Medical Journal 2011年第20期124卷 3347-3352页

作者： OU Qi-shui CheNG Zu-jian YANG Bin jianG Ling CheN Jing Department of Laboratory Medicine First Affiliated HospitalFujian Medical University Fuzhou Fujian 350005 China

Background It has been suggested that the ratio of mutant and wild type mitochondrial DNA may be related to its clinical phenotype. In this study, we developed a high sensitive real-time amplification refractory mutation system-quantitative polymerase chain reaction （RT-ARMS-qPCR） assay for quantitation of the mitochondrial DNA （mtDNA） with a mutated 1555 site, and explored the relationship between the ratio of mutated mtDNA and the severity of hearing loss of mitochondrial deafness （MD） patients of Fujian province in China. Methods An amplified mtDNA fragment containing the 1555 site was ligated into a vector to construct a plasmid DNA standard. An RT-ARMS-qPCR system was used to measure the mtDNA copy number containing wild-type and mutant sequences in a cohort of 126 MD patients of Fujian province in China. Combined with the clinical data, we explored the relationship between the ratio of mutated mtDNA and the severity of hearing loss of MD. Results The variation coefficient in the cohort was 1.21%, the interassay variation coefficient was 1.78%, and the linear range was 102-10e copies/pl for detecting a recombinant, wild-type plasmid. The primers amplified only the intended sequences. Mutation copy number correlated with the degree of deafness in sporadic cases with heteroplasmic mutations of mtDNA A1555G （R=0.811, P=0.003）, but not in sporadic cases with homoplasmic mutations （R=0.007, P=0.989）. The copy number of homoplasmic or heteroplasmic mutations of mtDNA A1555G in familial cases correlated with degree of deafness （R=0.352, P=0.023 and R=0.90, P=0.012, respectively）. Conclusions The RT-ARMS-qPCR system is suitable for determining the copy number of mtDNA fragments containing the A1555G mutation. The ratio of mutated mtDNA correlates with the severity of hearing loss of MD.

关键词： mitochondrial DNA A1555G real time-amplification refractory mutation system-quantitativepolymerase chain reaction deafness