Exploring the Mechanism of Tripterygium wilfordii Against Cancer Using Network Pharmacology and Molecular Docking

作     者：Shui-Xiu Xiao Shao-Jin Li Wan-Xian Fang Jv Chen Hai-Jian Li Yong-Li Situ 

作者机构：Obstetrics and GynecologyShenzhen Longhua District Central HospitalShenzhen 518110GuangdongChina Medical LaboratoryShenzhen Longhua District Central HospitalShenzhen 518110GuangdongChina Research DepartmentGuangzhou Darui Biotechnology Co.Ltd.Guangzhou 510670GuangdongChina Department of PharmacyAffiliated Hospital of Guangdong Medical UniversityZhanjiang 524023GuangdongChina Department of Clinical Laboratory MedicineGuangdong Second Provincial Central HospitalGuangzhou 510317GuangdongChina College of PharmacyJinan UniversityGuangzhou 510632GuangdongChina 

出 版 物：《World Journal of Traditional Chinese Medicine》 (世界中医药杂志（英文）)

年 卷 期：2022年第8卷第3期

页      面：417-425页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Guangzhou Concord Medical Humanities Research and Education Fund (23000-3050070) Project of Education Department of Guangdong Province (fund project of "Strengthening and Strengthening" of Guangdong Medical University in 2021)(4SG21202G) Science popularization project of Science and Technology Development Center of Chinese Pharmaceutical Society (CMEI2021KPYJ00310) 

主　　题：Tripterygium wilfordii cancer network pharmacology molecular docking 

摘      要：Background: The root of Tripterygium wilfordii(Tripterygii radix), a natural powerful traditional Chinese medicine(TCM) for various diseases treatment, has been used for centuries in the Asian countries as anti-rheumatoid arthritis(RA) agent, antioxidant agent, and anti-inflammatory agent. Its combination with other herbs in treating RA has been explored. The anti-RA effect of T. wilfordii for cancer treatment has been supported by some evidence. Aims and Objectives: To investigate the anticancer mechanism of T. wilfordii, bioinformatics databases were used to identify its active ingredients. Materials and Methods: Target proteins associated with cancer were determined using a network pharmacology analysis platform, and 25 key active compounds and 55 key targets of T. wilfordii were identified in our study. A common potential mechanism of T. wilfordii involvement in cancer was disclosed by in-depth network analysis of diseases, functions, and ***, the analysis results of the TCM-disease target protein interaction network revealed 5 potential targets;subsequently, a total of 30targets(these 5 targets, as well as 25 previously identified compounds) were subjected to molecular docking. Results: Our results showed that the therapeutic effect of T. wilfordii in cancer is characterized by multiple components, targets, and pathways. The regulation of signaling pathways such as Kaposi sarcoma-associated herpes virus infection, colorectal cancer, small-cell lung cancer, and prostate cancer may be the important pharmacodynamic basis of anticancer therapy. Conclusion: Triptonoditerpenic acid inhibited proliferation and induced apoptosis in SW480 cells. The mechanism may be related to the downregulation of Bcl-2 expression, upregulation of Bax mRNA expression, and expression inhibition of PTGS2.