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Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications

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Journal of Innovative Optical Health Sciences 2019年第5期12卷 79-85页

作者： Mengjie Zhao shuai ye Xiao Peng Jun Song Junle Qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060P.R.China

Inorganic quantum dots(QDs)have excellent optical properties,such as high°uorescence intensity,excellent photostability and tunable emission wavelength,etc.,facilitating them to be used as labels and probes for *** this study,CdSe@ZnS QDs are used as probes for Fluorescence lifetime imaging microscope(FLIM)and stimulated emission depletion(STED)nanoscopy *** emission peak of CdSe@ZnS QDs centered at 526 nm with a narrow width of 19 nm and the photoluminescence quantum yield(PLQY)was 64%.The QDs presented excellent anti-photobleaching property which can be irradiated for 400 min by STED laser with 39.8 *** lateral resolution of 42.0 nm is demonstrated for single QDs under STED laser(27.5 mW)***,the CdSe@ZnS QDs were for the first time used to successfully label the lysosomes of living HeLa cells and 81.5 nm lateral resolution is obtained indicating the available super-resolution applications in living cells for inorganic QD ***,Eca-109 cells labeled with the CdSe@ZnS QDs was observed with FLIM,and their fluorescence lifetime was around 3.1 ns,consistent with the in vitro value,suggesting that the QDs could act as a satisfactory probe in further FLIM-STED experiments.

关键词： CdSe@ZnS QDs FLIM STED living cells

Memory Trace for Fear Extinction:Fragile yet Reinforceable

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Neuroscience Bulletin 2024年第6期40卷 777-794页

作者： Ying Liu shuai ye Xin-Ni Li Wei-Guang Li Department of Rehabilitation Medicine State Key Laboratory of Medical Neurobiology and Ministry of Education Frontiers Center for Brain ScienceHuashan HospitalInstitute for Translational Brain ResearchFudan UniversityShanghai200032China

Fear extinction is a biological process in which learned fear behavior diminishes without anticipated reinforcement,allowing the organism to re-adapt to ever-changing *** on the behavioral hypothesis that extinction is new learning and forms an extinction memory,this new memory is more readily forgettable than the original fear *** brain’s cellular and synaptic traces underpinning this inherently fragile yet reinforceable extinction memory remain *** questions are about the whereabouts of the engram neurons that emerged during extinction learning and how they constitute a dynamically evolving functional construct that works in concert to store and express the extinction *** this review,we discuss recent advances in the engram circuits and their neural connectivity plasticity for fear extinction,aiming to establish a conceptual framework for understanding the dynamic competition between fear and extinction memories in adaptive control of conditioned fear responses.

关键词： Fear extinction memory Memory trace Fear relapse Medial prefrontal cortex Basolateral amygdala Ventral hippocampus Insular cortex Synaptic adaptations

Biocompatible carbon dots with low-saturation-intensity and highphotobleaching-resistance for STED nanoscopy imaging of the nucleolus and tunneling nanotubes in living cells

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Nano Research 2019年第12期12卷 3075-3084页

作者： Hao Li shuai ye Jiaqing Guo Huibo Wang Wei Yan Jun Song Junie Qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College cf Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices Institute of Functional Nano and Soft Materials(FUNSOM)Soochow UniversitySuzhou 215123China

Many kinds of nano particles and organic dyes as fluorescent probes have been used in the stimulated emission depletion(STED)*** to high toxicity,photobleaching and non-water solubility,these fluorescent probes are hard to apply in living cell ***,we reporta new fluorescence carbon dots(FNCDs)with high photoluminescence quantum yield(56%),low toxicity,anti-photobleaching and goodwater-solubility that suitable for live-cell imaging can be obtained by doping fluorine ***,the FNCDs can stain the nucleolusand tunneling nanotubes(TNTs)in the living *** importantly,for STED nanoscopy imaging,the FNCDs effectively depleted backgroundsignals and improved imaging ***,the lateral resolution of single FNCDs size under the STED nanoscopy is up to 22.1 nm for FNCDs deposited on a glass slide was *** because of their good water dispersibility,the higher resolution of single FNCDs sizein the nucleolus of a living cell can be up to 19.7 *** the image optimizati on steps,the fine fluoresce nee images of TNTs diameter with ca.75 nm resolution is obtained living cell,yielding a threefold enhancement compared with that in confocal ***,the FNCDs show excellent photobleaching resistance after 1,000 scan cycles in the STED *** results show that FNCDs have significant potentialfor application in STED nanoscopy.

关键词： carbon dots excellent photobleaching resistance high resolution stimulated emission depletion(STED)nanoscopy tunneling nanotubes

Switching Behavior Induced by Electric and Magnetic Fields in （La0.73Bi0.27）0.67Ca0.33MnO3

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Chinese Physics Letters 2014年第4期31卷 159-162页

作者： WANG ye-shuai XIA Nian-Ming ZUO Hua-Kun SHEN Yi-Ning XIA Zheng-Cai Wuhan National High Magnetic Field Center Huazhong University of Science and Technology Wuhan 430074

Both electric and magnetic field-induced switching behaviors between a high resistive state and a low resistive one are observed in （La0.73Bi0.27）0.67Ca0.33MnO3. The effects of magnetoresistance *** electric-resistance suggest that the applied electric field and magnetic field greatly tune the percolative paths in the phase-separated system. According to the experimental results, the switching behaviors may come from the coexistence of the charge ordering state, and localized and freedom ferromagnetic states, in which the external field destroys partially the localized ferromagnetic states and charge ordering leads to the ferromagnetic state growth, which causes a switch between a high resistive state and low resistive one. This makes the doped manganite a good system for both electric and magnetic field sensor materials.

关键词： SWITCHING circuits MAGNETIC fields ELECTRIC resistance FERROMAGNETISM MAGNETORESISTANCE

Self-powered and bipolar photodetector based on a van der Waals metal-semiconductor junction: Graphene/WSe;/Fe;GeTe;heterojunction

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Science China Technological Sciences 2022年

作者： XU Guo Liang LIU Dan Min LI Jing Zhen LI Jing Jie ye shuai shuai Key Laboratory of Advanced Functional Materials Ministry of Education Faculty of Materials and Manufacturing Beijing University of Technology Key Laboratory of Optoelectronics Technology College of Microelectronics Faculty of Information Technology Beijing University of Technology

A self-driven photosensor with signal-reversal response has the potential to work as a photodetector in complex environments with multiple signals owing to better signal recognition and enhanced signal-processing efficiency. Herein, a metal-semiconductor-metal photodetector based on ambipolar WSe;with two electrodes comprising two-dimensional（2D） van der Waals（vd Ws） metal Fe;GeTe;and semimetal graphene was proposed to form an asymmetrical metal-contacted architecture with different Schottky barrier heights. The regulating gate field-induced Fermi level shift in WSe;can be used to manipulate the WSe;channel’s carrier type, resulting in a polarity-reversible photodetector without a bias voltage. Furthermore, the photovoltaic effect can be observed from wavelengths of 450 nm（visible） to 850 nm（infrared） without external voltage. The large open voltage is-0.177 V and short-circuit current is 17 n A under a 650-nm excitation wavelength. The reported WSe;-based photodetector exhibits excellent properties under zero bias, including a large photo-to-dark current ratio greater than 106 with dark current less than 1 f A, photovoltaic performance with an external quantum efficiency of 27.14%, an excellent detectivity of 3.4×1010Jones, and a high responsivity of 116.38 m A/W. Rapid electron transfer occurs at the interface between WSe;and vd Ws electrodes, resulting in a 370-μs response speed owing to the clean and nondestructive interfaces between vd Ws metals and WSe;. This study demonstrates the wide application prospect of the vd Ws metal Fe;GeTe;as an electrode forming a Schottky junction for realizing a photodetector without an external voltage and accelerates the development of 2D photosensors with various working modes.

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金代博弈活动的初步研究——以考古材料为中心

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黑龙江民族丛刊 2018年第4期 61-67页

作者：叶帅南京大学历史学院南京210093

"博弈"语出自《论语·阳货》,指六博和围棋,后引申为博戏弈棋类活动的统称。随着金朝的建立及疆域的扩展,世居东北地区的女真人开始接受和吸纳辽、宋的物质文化,流行于辽、宋的多种博弈活动也包括在其中。结合史料和考古发现综合考察,... 详细信息

"博弈"语出自《论语·阳货》,指六博和围棋,后引申为博戏弈棋类活动的统称。随着金朝的建立及疆域的扩展,世居东北地区的女真人开始接受和吸纳辽、宋的物质文化,流行于辽、宋的多种博弈活动也包括在其中。结合史料和考古发现综合考察,金代博弈活动主要有双陆、围棋、象棋、骰子、骨牌、鹿棋、连棋等。金代博弈活动种类繁多、流传广泛,并有着一定的群体性特征和较高的女性参与度。

关键词：金代博弈活动围棋象棋骰子骨牌

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太湖庄园闸站前池流态分析及改善措施

水电能源科学 2023年第9期41卷 194-196,163页

作者：叶帅无锡市政设计研究院有限公司江苏无锡214072

为探究常州市太湖庄园闸站在引水工况时前池存在流态问题的起因,并找到相应的解决办法,通过三维紊流数值模拟方法模拟闸站原型及增设导流墩后的进水流态,分析各情况下进水断面流速均匀度和加权平均角可知,前池流态问题主要是因其扩散角... 详细信息

为探究常州市太湖庄园闸站在引水工况时前池存在流态问题的起因,并找到相应的解决办法,通过三维紊流数值模拟方法模拟闸站原型及增设导流墩后的进水流态,分析各情况下进水断面流速均匀度和加权平均角可知,前池流态问题主要是因其扩散角偏大所致,在前池增设单根导流墩越长,整流效果越好,组合式导流墩相比于单根导流墩,更能削弱回流区,改善进水流态。研究结果可为工程实践提供参考。

关键词：前池大扩散角流态导流墩数值模拟

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竞技蹦床训练方法与应用研究

沈阳体育学院学报 2021年第1期40卷 79-84,93页

作者：刘兴叶帅沈阳体育学院体育教育学院辽宁沈阳110102 福建体育职业技术学院社会体育系福建福州350003

蹦床是我国竞技体育后发优势项目,男女均处于世界先进水平,但理论研究相对滞后,还没有建立和形成较为完整的项目理论体系,与训练实践水平不相称。训练方法是蹦床训练理论的核心内容之一,也是训练实践的关键要素。中国蹦床经过20余年的... 详细信息

蹦床是我国竞技体育后发优势项目,男女均处于世界先进水平,但理论研究相对滞后,还没有建立和形成较为完整的项目理论体系,与训练实践水平不相称。训练方法是蹦床训练理论的核心内容之一,也是训练实践的关键要素。中国蹦床经过20余年的发展实践,已经形成了一整套比较成熟的技术训练方法与实践体系。在实践总结、理论分析和访谈征询的基础上,首次提出了地网与高网结合训练法、陆地基本姿态训练法、专项能力与技术训练结合法、多成套与专项能力训练法、多网变换训练法和男女组合训练法等6种蹦床训练方法。研究成果对于构建和完善我国蹦床项目训练理论体系具有重要参考价值。

关键词：竞技蹦床训练方法训练理论应用研究

数据、现象与理论——兼评波根和伍德沃德的数据哲学思想

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自然辩证法研究 2015年第6期31卷 106-111页

作者：叶帅张怡东华大学人文学院上海201620

观察与理论严格区分作为逻辑实证主义的基本假设以及经验主义赖以生存的核心论题,饱受诟病。波根(***)和伍德沃德(***)提出数据-现象-理论三元区分以代替传统的二元区分,突显出数据在科学中的作用。但是这一模式在数据理论负载、数据-... 详细信息

观察与理论严格区分作为逻辑实证主义的基本假设以及经验主义赖以生存的核心论题,饱受诟病。波根(***)和伍德沃德(***)提出数据-现象-理论三元区分以代替传统的二元区分,突显出数据在科学中的作用。但是这一模式在数据理论负载、数据-现象推论有效性、现象与理论严格区分等方面仍然存在着不可调和的困难。以此本文力图给出数据(Data)、现象(Phenomena)和理论(Theory)的三角模型(D-P-T三角模型)的重新解释,认为三者之间呈现互相制约的动态关系,数据不仅为现象提供证据,也为证实待测理论提供证据;数据、现象与理论之间无法在根本上做出严格区分。

关键词：观察和理论数据现象 D-P-T三角模型