Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications
作者机构：Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060P.R.China
出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)
年 卷 期：2019年第12卷第5期
页 面：79-85页
核心收录：
学科分类：07[理学] 070201[理学-理论物理] 0702[理学-物理学]
基 金：supported by the National Key R&D Program of China(2018YFC0910600) the National Natural Science Foundation of China(61605124/41603059/61525503/61620106016/61835009/81727804) Project of Department of Education of Guangdong Province(2015KGJHZ002/2016KCXTD007) Guangdong Natural Science Foundation Innovation Team(2014A030312008) Shenzhen Basic Research Project(JCYJ20150930104948169/JCYJ20160328144746940/JCYJ20170412105003520) the Natural Science Foundation of Shenzhen University(2019108)
主 题：CdSe@ZnS QDs FLIM STED living cells
摘 要：Inorganic quantum dots(QDs)have excellent optical properties,such as high°uorescence intensity,excellent photostability and tunable emission wavelength,etc.,facilitating them to be used as labels and probes for *** this study,CdSe@ZnS QDs are used as probes for Fluorescence lifetime imaging microscope(FLIM)and stimulated emission depletion(STED)nanoscopy *** emission peak of CdSe@ZnS QDs centered at 526 nm with a narrow width of 19 nm and the photoluminescence quantum yield(PLQY)was 64%.The QDs presented excellent anti-photobleaching property which can be irradiated for 400 min by STED laser with 39.8 *** lateral resolution of 42.0 nm is demonstrated for single QDs under STED laser(27.5 mW)***,the CdSe@ZnS QDs were for the first time used to successfully label the lysosomes of living HeLa cells and 81.5 nm lateral resolution is obtained indicating the available super-resolution applications in living cells for inorganic QD ***,Eca-109 cells labeled with the CdSe@ZnS QDs was observed with FLIM,and their fluorescence lifetime was around 3.1 ns,consistent with the in vitro value,suggesting that the QDs could act as a satisfactory probe in further FLIM-STED experiments.
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