Biocompatible carbon dots with low-saturation-intensity and highphotobleaching-resistance for STED nanoscopy imaging of the nucleolus and tunneling nanotubes in living cells
作者机构：Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege cf Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China Jiangsu Key Laboratory for Carbon-based Functional Materials and DevicesInstitute of Functional Nano and Soft Materials(FUNSOM)Soochow UniversitySuzhou 215123China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2019年第12卷第12期
页 面：3075-3084页
核心收录：
学科分类：08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学]
基 金：We thank X.Peng(Shenzhen University)for great assistance in tunneling nanotubes of live cell.This work was partially supported by the National Key R&D Program of China(No.2018YFC0910600) the National Natural Science Foundation of China(Nos.61975132,61775145,61525503,61620106016,61835009,and 81727804) China Postdoctoral Science Foundation(No.2019M650211) Guangdong Province Key Area R&D Program(No.2019B110233004) Project of Department of Education of Guangdong Province(No.2015KGJHZ002/2016KCXTD007) the Shenzhen Basic Research Project(Nos.JCYJ20170412110212234 and JCYJ20170412105003520) the Natural Science Foundation of Shenzhen University(2019108)
主 题：carbon dots excellent photobleaching resistance high resolution stimulated emission depletion(STED)nanoscopy tunneling nanotubes
摘 要：Many kinds of nano particles and organic dyes as fluorescent probes have been used in the stimulated emission depletion(STED)*** to high toxicity,photobleaching and non-water solubility,these fluorescent probes are hard to apply in living cell ***,we reporta new fluorescence carbon dots(FNCDs)with high photoluminescence quantum yield(56%),low toxicity,anti-photobleaching and goodwater-solubility that suitable for live-cell imaging can be obtained by doping fluorine ***,the FNCDs can stain the nucleolusand tunneling nanotubes(TNTs)in the living *** importantly,for STED nanoscopy imaging,the FNCDs effectively depleted backgroundsignals and improved imaging ***,the lateral resolution of single FNCDs size under the STED nanoscopy is up to 22.1 nm for FNCDs deposited on a glass slide was *** because of their good water dispersibility,the higher resolution of single FNCDs sizein the nucleolus of a living cell can be up to 19.7 *** the image optimizati on steps,the fine fluoresce nee images of TNTs diameter with ca.75 nm resolution is obtained living cell,yielding a threefold enhancement compared with that in confocal ***,the FNCDs show excellent photobleaching resistance after 1,000 scan cycles in the STED *** results show that FNCDs have significant potentialfor application in STED nanoscopy.
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