检索结果-南通市图书馆

Science China(Life Sciences) 2023年第6期66卷 1340-1357页

作者： Weiwei Zheng xing Lv shiying xin Yuena Sun Tianjun Xu Laboratory of Fish Molecular Immunology College of Fisheries and Life ScienceShanghai Ocean UniversityShanghai201306China Laboratory of Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266237China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Shanghai Ocean UniversityMinistry of EducationShanghai201306China National Pathogen Collection Center for Aquatic Animals Shanghai Ocean UniversityShanghai201306China

Noncoding RNA(ncRNA)is an important regulatory factor that plays a major role in innate ***,most studies on ncRNA have focused on mammals,resulting in a knowledge gap on nc RNA in lower vertebrates such as teleost *** this study,we identified a new long noncoding RNA(lncRNA),termed TAK1-related lnc RNA(TARL),which can play a positive role in the antibacterial immunity of Miichthys miiuy to Vibrio anguillarum and *** also found a novel micro RNA miR-2188-3p that could target TAK1 and inhibit the host antibacterial response and promote bacterial *** further found that the antibacterial effect inhibited by miR-2188-3p could be reversed with ***,*** and *** are the two most susceptible Gram-negative pathogens of aquaculture fish,and the economic losses caused by these two bacteria are immeasurable every *** study is the first to report on the ability of lnc RNA to prevent the escape of *** and *** in fish through the competing endogenous RNA(ceRNA)*** results not only elucidate the ce RNA mechanism of the lncRNA in antibacterial immune responses but also provide new insights into the impact of lnc RNA on host immunity and bacterial escape.

关键词： lncRNA microRNA ceRNA innate immunity antibacterial

LncRNA MIR144HG-derived miR-144 suppresses antibacterial signaling and facilitates bacteria escape in fish

在线全文

学校读者我要写书评

暂无评论

Water Biology and Security 2023年第1期2卷 72-85页

作者： Weiwei Zheng Junxia Cui xing Lv shiying xin Tianjun Xu Laboratory of Fish Molecular Immunology College of Fisheries and Life ScienceShanghai Ocean UniversityShanghai201306China Laboratory of Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266200China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University) Ministry of EducationShanghai201306China National Pathogen Collection Center for Aquatic Animals Shanghai Ocean UniversityShanghai201306China

Growing number studies show that lncRNA is an important regulatory factor and plays an irreplaceable role in innate ***,most studies on lncRNA have focused on mammals and it is unknown in lower *** this study,we identify a new lncRNA,named MIR144HG,which can have a negative role in antibacterial immunity of Miichthys miiuy caused by Vibrio anguillarum and ***88,TAK1 and p65 are very important pathway genes in antibacterial innate immune responses,whether in mammals or lower ***,we found that miR-144–3p could target MyD88,TAK1 and p65,inhibit host antibacterial response and promote bacterial *** studies found that miR-144–3p can be derived by MIR144HG,and finally enhance the inhibitory effect of miR-144–3p on host antibacterial ***,*** and *** are the two most susceptible Gram-negative pathogens in aquaculture,and the economic losses caused by these two bacteria are immeasurable every *** results not only elucidate the mechanism of the lncRNAmiRNA-mRNA axis in antibacterial immune responses,but also provide new insights for understanding the impact of lncRNA on host immunity and bacteria escape.

关键词： Antibacterial Fish Innate immunity lncRNA microRNA Precursor

高量秸秆不同深度还田对黑土有机质组成和酶活性的影响

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

土壤学报 2015年第3期52卷 665-672页

作者：矫丽娜李志洪殷程程王晓飞辛士颖于磊吉林农业大学资源与环境学院长春130118 黑龙江省科学院大庆分院黑龙江大庆163319

在田间耕作条件下黑土3个土层（0—20、20。40、40～60cm）添加4％和8％高量玉米秸秆于尼龙袋中原位培养近4年后，研究不同层次土壤秸秆转化与有机碳积累特征，以及腐殖质各组分和土壤酶活性的变化。结果表明，添加14％的秸秆量0—20、2... 详细信息

在田间耕作条件下黑土3个土层（0—20、20。40、40～60cm）添加4％和8％高量玉米秸秆于尼龙袋中原位培养近4年后，研究不同层次土壤秸秆转化与有机碳积累特征，以及腐殖质各组分和土壤酶活性的变化。结果表明，添加14％的秸秆量0—20、20～40和40～60cm土层有机碳分别增加31．8％、96．4％和171．1％，8％秸秆添加量分别增加了86．2％、193．5％和265．9％，增加秸秆还田深度有利于土壤有机碳的积累。0～20cm土层在无秸秆还田情况下有机碳下降了29-3％，而20～40cm土层仅下降了1．8％。土壤有机碳含量和酶活性均随秸秆添加量的增加而提高，腐殖质胡敏酸／富里酸（HA／FA）比值发生较大变化，改善了腐殖质品质。各处理腐殖酸碳（HS—C）和胡敏酸碳（HA—C）的大小为20～40cm土层〉0～20cm土层〉40～60cm土层，而40～60cm土层则更有利于富里酸碳（FA—c）的积累。土壤过氧化氢酶活性、脲酶和蔗糖酶活性分别与HA—C、FA—C含量呈极显著正相关、显著正相关。研究结果为深层秸秆还田促进土壤有机质的积累提供了理论依据。

关键词：秸秆还田土层腐殖质积累特征土壤酶活性

A novel advanced oxidation process to degrade organic pollutants in wastewater:Microwave-activated persulfate oxidation

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2009年第9期21卷 1175-1180页

作者： YANG shiying WANG Ping YANG xin WEI Guang ZHANG Wenyi SHAN Liang Key Laboratory of Marine Environment & Ecology Ministry of Education Qingdao 266100 China. College of Environmental Science and Engineering Ocean University of China Qingdao 266100 China. Qingdao Huashan Biochemical Company Limited Qingdao 266216 China

This article, for the first time, provides a novel advanced oxidation process based on sulfate radical （SO^4·-） to degrade organic pollutants in wastewater： microwave （MW）-activated persulfate oxidation （APO） with or without active carbon （AC）. Azo dye acid Orange 7 （AO7） is used as a model compound to investigate the high reactivity of MW-APO. It is found that AO7 （up to 1000 mg/L） is completely decolorized within 5-7 min under an 800 W MW furnace assisted-APO. In the presence of chloride ion （up to 0.50 mol/L）, the decolorization is still 100% completed, though delayed for about 1-2 min. Experiments are made to examine the enhancement by AC. It is exciting to find that the 100% decolorization of AO7 （500 mg/L） is achieved within 3 min by MW-APO using 1.0 g/L AC as catalyst, while the degradation efficiency maintains at 50% by MW energy without persulfate after about 5 min. Besides the destruction of visible light chromophore band of AO7 （484 nm）, during MW-APO, two bands in the ultraviolet region （228 nm and 310 nm） are rapidly broken down. The removal of COD is about 83%-95% for 500 mg/L AO7. SO^4·- is identified with quenching studies using specific alcohols. Both SO^4·- and ·OH could degrade AO7, but SO^4·- plays the dominant role. In a word, MW-APO AC is a new catalytic combustion technology for destruction of organic contamination even for high concentration.

关键词： microwave-activated persulfate oxidation active carbon sulfate radical advanced oxidation technology

The Genetics of Clinical Liver Diseases: Insight into the TM6SF2 E167K Variant

在线全文

学校读者我要写书评

暂无评论

Journal of Clinical and Translational Hepatology 2018年第3期6卷 326-331页

作者： Xiaoyu Zhang Shousheng Liu Quanjiang Dong Yongning xin shiying Xuan Department of Gastroenterology Taishan Medical UniversityTaianChina Department of Infectious Disease Qingdao Municipal HospitalQingdaoChina Department of Gastroenterology Qingdao Municipal HospitalQingdaoChina Digestive Disease Key Laboratory of Qingdao QingdaoChina Central Laboratories Qingdao Municipal HospitalQingdaoChina

The transmembrane 6 superfamily member 2(TM6SF2)gene E167K variant(rs58542926)was identified by exome-wide as-sociation study as a nonsynonymous single nucleotide poly-morphism associated with nonalcoholic fatty liver *** TM6SF2 E167K variant features a C-to-T substitution at nucleotide 499,encoding a glutamate with lysine change at codon 167(E167K).TM6SF2 is markedly expressed in the liver,small intestine and kidney,and has been proposed as an im-portant risk factor for diseases associated with lipid ***,multifunctional studies of the TM6SF2 E167K variant have been carried out in a spectrum of liver dis-eases,such as nonalcoholic fatty liver disease,nonalcoholic steatohepatitis,fibrosis,cirrhosis,and viral *** re-view summarizes the research status of the TM6SF2 E167K variant in different liver diseases and specific populations,and discusses the potential mechanisms of the TM6SF2 E167K var-iant's role in the progression of various liver diseases.

关键词： TM6SF2 SNP NAFLD Fibrosis Cirrhosis Virus hepatitis

Tenofovir Alafenamide Fumarate,Tenofovir Disoproxil Fumarate and Entecavir:Which is the Most Effective Drug for Chronic Hepatitis B?A Systematic Review and Meta-analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Clinical and Translational Hepatology 2021年第3期9卷 335-344页

作者： Xuefeng Ma Shousheng Liu Mengke Wang Yifen Wang Shuixian Du Yongning xin shiying Xuan Department of Infectious Disease Qingdao Municipal HospitalQingdao UniversityQingdaoShandongChina Clinical Research Center Qingdao Municipal HospitalQingdao UniversityQingdaoShandongChina Digestive Disease Key Laboratory of Qingdao QingdaoShandongChina

Background and Aims:The therapeutic effect of tenofovir alafenamide fumarate(TAF),tenofovir disoproxil fumarate(TDF)and entecavir(ETV)on chronic hepatitis B(CHB)patients remains *** aim of this study was to explore the differences in virological responses to TAF,TDF and ETV in patients with ***:Literature searches were conducted of the PubMed,EMBASE,and the Cochrane Library databases to identify randomized controlled trials and observational studies published up to July 21,*** comparisons of virological response between TDF,ETV,and TAF were carried out with pooled odds ratio(OR)***:The virological response in TDF-treated CHB patients was notably superior to that of the ETV-treated CHB patients after 12-weeks[OR=1.12,95%confidence interval(CI):0.89–1.41],24-weeks(OR=1.33,95%CI:1.11–1.61),48-weeks(OR=1.62,95%CI:1.16–2.25),72-weeks(OR=1.43,95%CI:0.78–2.62),and 96-weeks(OR=1.56,95%CI:0.87–2.81)*** significant difference was observed for the virological responses in CHB patients after 48-weeks treatment with TAF or *** virological response in TDF+ETV-treated CHB patients was superior to that of TDF-treated CHB patients after 24-weeks,48-weeks(OR=1.54,95%CI:1.17–2.02),96-weeks,and ***:The virological response in TDF-treated CHB patients was superior to that in ETV-treated CHB patients,but there was no significant difference between TAF and *** addition,the therapeutic effect of TDF+ETV was superior to TDF alone.

关键词： Tenofovir alafenamide Entecavir Tenofovir disoprox Chronic hepatitis B Virological response

Noninvasive Quantitative Detection Methods of Liver Fat Content in Nonalcoholic Fatty Liver Disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Clinical and Translational Hepatology 2018年第2期6卷 217-221页

作者： Shujing Lv Sushan Jiang Shousheng Liu Quanjiang Dong Yongning xin shiying Xuan Medical College of Qingdao University QingdaoShandongChina Digestive Disease Key Laboratory of Qingdao QingdaoShandongChina Central Laboratories Qingdao Municipal HospitalQingdaoShandongChina Department of Gastroenterology Qingdao Municipal HospitalQingdaoShandongChina Department of Infectious Disease Qingdao Municipal HospitalQingdaoShandongChina

Nonalcoholic fatty liver disease(NAFLD)ranges from simple steatosis to NAFLD-related liver cirrhosis and is a main cause of chronic liver *** with nonalcoholic steatohe-patitis and fibrosis are at a great risk of the progression to cirrhosis or hepatocellular carcinoma,both of which are tightly associated with liver-related *** biopsy is still the gold standard for the diagnosis of NAFLD,but some defects,such as serious complications,sampling error and variability in histologic evaluation among pathologists,remain ***,noninvasive,repeatable and accurate diagnostic methods are urgently *** is a well-established and lower-cost imaging technique for the diagnosis of hepatic steatosis,especially suitable for population census,but limited by its low sensitivity to diagnose mild steatosis and being highly *** tomography also lacks the sensitivity to detect mild steatosis and small changes in fat content,and presents a potential radiation *** attenuation parameter based on the FibroScan?technology is a promising tool for noninvasive semiquantita-tive assessment of liver fat content,but the accuracy rate de-pends on the operator's expertise and is affected by age,width of the intercostal space,skin capsular distance and body mass *** resonance imaging and magnetic resonance spectroscopy are regarded as the most accurate quantitative methods for measuring liver fat content in clinical practice,especially for longitudinal follow up of NAFLD *** this review,we mainly introduce the current imaging methods that are in use for evaluation of liver fat content and we discuss the advantages and disadvantages of each method.

关键词： Nonalcoholic fatty liver disease Hydrogen-1 magnetic resonance spectroscopy Noninvasive Liver fat content

Antiviral Effects of Stichopus japonicus Acid Mucopolysaccharide on Hepatitis B Virus Transgenic Mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2016年第4期15卷 719-725页

作者： xin Yongning LI Wei LU Linlin ZHOU Li David W Victor XUAN shiying Qingdao Municipal Hospital Qingdao 266071 P. R. China Department of Gasteroenterology Qingdao No.6 People's Hospital Qingdao 266033 P. R. China Taiyuan Infectious Diseases Hospital Taiyuan 030000 P. R. China Hepatology & Transplant Medicine Department of Medicine Houston Methodist Hospital Houston USA

Hepatitis B virus(HBV) is a significant global pathogen and efficient cure for HBV patients is still a challenging goal. We previously reported that acidic mucopolysaccharide from stichopus japonicus selenka(SJAMP) could inhibit HBs Ag and HBe Ag expression in vitro. However, the potential anti-HBV effects of SJAMP in vivo have not yet been explored. In this study, we show that SJAMP exhibits potent anti-HBV activity in HBV transgenic mice in a dose-dependent manner. Specifically, sixty HBV transgenic male BALB/c mice were randomly selected to receive the treatment of PBS, low dose SJAMP(30 mg kg^(-1)), middle dose SJAMP(40 mg kg^(-1)), high dose SJAMP(50 mg kg^(-1)) and IFN(45 IU kg^(-1)) for 30 d. SJAMP treatment suppressed serum HBV-DNA, and liver HBs Ag and HBc Ag levels in HBV-transgenic mice. The present study highlights the potential application of SJAMP in HBV therapy.

关键词： Transgenic transgenic pathogen challenging suppressed potent highlights brown manner inhibit

Association of GCKR Gene Polymorphisms with the Risk of Nonalcoholic Fatty Liver Disease and Coronary Artery Disease in a Chinese Northern Han Population

在线全文

学校读者我要写书评

暂无评论

Journal of Clinical and Translational Hepatology 2019年第4期7卷 297-303页

作者： Hui Gao Shousheng Liu Zhenzhen Zhao xinjuan Yu Qun Liu Yongning xin shiying Xuan Department of Infectious Disease Qingdao Municipal HospitalQingdao UniversityQingdaoShandongChina Central Laboratories Qingdao Municipal HospitalQingdao UniversityQingdaoShandongChina Department of Gastroenterology Qingdao Municipal HospitalQingdao UniversityQingdaoShandongChina Digestive Disease Key Laboratory of Qingdao QingdaoShandongChina

Background and Aims: Accumulated studies have eval-uated the effects of glucokinase regulatory protein(GCKR)gene polymorphisms on the risk of nonalcoholic fatty liver disease(NAFLD)and coronary artery disease(CAD),but the association of GCKR polymorphisms with the risk of NAFLD and CAD in the Chinese Han population have remained *** aim of this study was to investigate the association between GCKR gene polymorphisms(rs780094 and rs1260326)and the risk of NAFLD and CAD in NAFLD patients in a Chinese Northern Han ***: GCKR rs780094 and rs1260326 gene polymorphisms were geno-typed by polymerase chain reaction sequencing for B-type ultrasonography-proven NAFLD patients with(n = 82)or without(n = 142)CAD,and in healthy controls(n = 152).Serum lipid profiles'levels were determined using biochemi-cal *** analyses were conducted using SPSS 22.0 statistical ***: As the results showed,sig-nificant differences in the serum lipid profiles existed between each *** significant differences were observed in the distributions of genotypes and alleles of GCKR rs780094 and rs1260326 in each *** GCKR rs780094 T and rs1260326 T allele carriers possessed decreased body mass index value,and serum fasting plasma glucose and TG levels in the overall subjects,*** addition,the GCKR rs780094 T allele carriers possessed decreased serum fasting plasma glucose level in the controls and NAFLD+CAD ***: GCKR rs780094 and rs1260326 poly-morphisms were found to be not associated with the risk of NAFLD nor of CAD in NAFLD patients in this Chinese Northern Han *** rs780094 T and rs1260326 T alleles could affect the body mass index value and serum fasting plasma glucose and triglyceride levels.

关键词： Non-alcoholic fatty liver disease Glucokinase regulatory protein gene Polymorphism Coronary artery disease

Microstructural evolution and FCC twinning behavior during hot deformation of high temperature titanium alloy Ti65

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2020年第14期47卷 56-69页

作者： Zhixin Zhang Jiangkun Fan Bin Tang Hongchao Kou Jian Wang xin Wang shiying Wang Qingjiang Wang Zhiyong Chen Jinshan Li State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’anShaanxi710072China National&Local Joint Engineering Research Center for Precision Thermoforming Technology of Advanced Metal Materials Xi’anShaanxi710072China Baoti Group Ltd. BaojiShaanxi721014China School of Materials Science and Engineering Changzhou UniversityChangzhouJiangsu213164China Institute of Metal Research Chinese Academy of SciencesShenyangLiaoning 110016China

Although the development of titanium alloys with working temperatures above 600?C faces enormous difficulties and challenges,the related research has not *** the present work,detailed analyses on microstructure evolution and hot deformation behavior of a new temperature resistant 650?C titanium alloy Ti65 were investigated from micrometer scale to nanometer *** results revealed that lamellarαgrains gradually fragmentized and spheroidized during theα+βphase region compression and the orientation of the c-axis ofαgrains gradually aligned to radial directions,forming two high Schmid factors(SFs)value texture eventually with the increase of strain to ***,there were some strengthening characters in theα+βphase region such as lenticularαsand nano silicide(TiZr)6 *** theβphase region,fine equiaxed dynamic recrystallized(DRX)βgrains were ***,the variant selection ofαm′artensite followed Burgers orientation relationship during the compression *** main deformation mechanisms of theα+βphase region were dislocation slip and orientation dependent ***,the deformation process in theβphase region was controlled byβgrain ***,many nano scale FCC twins were generated at the interface ofαl′ath during deforming in theβphase region,which was firstly observed in Ti65 alloy.

关键词： High temperature titanium alloy Hot deformation Microstructure evolution Texture FCC twin