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Science China(Life Sciences) 2023年第6期66卷 1340-1357页

作者： Weiwei Zheng xing Lv shiying xin Yuena Sun Tianjun Xu Laboratory of Fish Molecular Immunology College of Fisheries and Life ScienceShanghai Ocean UniversityShanghai201306China Laboratory of Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266237China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Shanghai Ocean UniversityMinistry of EducationShanghai201306China National Pathogen Collection Center for Aquatic Animals Shanghai Ocean UniversityShanghai201306China

Noncoding RNA(ncRNA)is an important regulatory factor that plays a major role in innate ***,most studies on ncRNA have focused on mammals,resulting in a knowledge gap on nc RNA in lower vertebrates such as teleost *** this study,we identified a new long noncoding RNA(lncRNA),termed TAK1-related lnc RNA(TARL),which can play a positive role in the antibacterial immunity of Miichthys miiuy to Vibrio anguillarum and *** also found a novel micro RNA miR-2188-3p that could target TAK1 and inhibit the host antibacterial response and promote bacterial *** further found that the antibacterial effect inhibited by miR-2188-3p could be reversed with ***,*** and *** are the two most susceptible Gram-negative pathogens of aquaculture fish,and the economic losses caused by these two bacteria are immeasurable every *** study is the first to report on the ability of lnc RNA to prevent the escape of *** and *** in fish through the competing endogenous RNA(ceRNA)*** results not only elucidate the ce RNA mechanism of the lncRNA in antibacterial immune responses but also provide new insights into the impact of lnc RNA on host immunity and bacterial escape.

关键词： lncRNA microRNA ceRNA innate immunity antibacterial

LncRNA MIR144HG-derived miR-144 suppresses antibacterial signaling and facilitates bacteria escape in fish

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Water Biology and Security 2023年第1期2卷 72-85页

作者： Weiwei Zheng Junxia Cui xing Lv shiying xin Tianjun Xu Laboratory of Fish Molecular Immunology College of Fisheries and Life ScienceShanghai Ocean UniversityShanghai201306China Laboratory of Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266200China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University) Ministry of EducationShanghai201306China National Pathogen Collection Center for Aquatic Animals Shanghai Ocean UniversityShanghai201306China

Growing number studies show that lncRNA is an important regulatory factor and plays an irreplaceable role in innate ***,most studies on lncRNA have focused on mammals and it is unknown in lower *** this study,we identify a new lncRNA,named MIR144HG,which can have a negative role in antibacterial immunity of Miichthys miiuy caused by Vibrio anguillarum and ***88,TAK1 and p65 are very important pathway genes in antibacterial innate immune responses,whether in mammals or lower ***,we found that miR-144–3p could target MyD88,TAK1 and p65,inhibit host antibacterial response and promote bacterial *** studies found that miR-144–3p can be derived by MIR144HG,and finally enhance the inhibitory effect of miR-144–3p on host antibacterial ***,*** and *** are the two most susceptible Gram-negative pathogens in aquaculture,and the economic losses caused by these two bacteria are immeasurable every *** results not only elucidate the mechanism of the lncRNAmiRNA-mRNA axis in antibacterial immune responses,but also provide new insights for understanding the impact of lncRNA on host immunity and bacteria escape.

关键词： Antibacterial Fish Innate immunity lncRNA microRNA Precursor

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贵金属催化剂低温催化氧化一氧化碳研究进展

功能材料 2024年第1期55卷 1060-1067页

作者：代欣任德志郭律朱敬芳于飞常仕英昆明贵金属研究所昆明650106 昆明贵研催化剂有限责任公司昆明650106 贵研催化剂(东营)有限公司山东东营257000

随着“双碳”政策的不断推行以及人们环保意识的不断提高,一氧化碳(CO)作为典型的大气污染物,已成为工业废气和汽车尾气排放的主要控制对象。贵金属型CO氧化催化剂具有优异的低温活性、抗中毒抗性能,是CO催化氧化处理的最为有效的手段... 详细信息

随着“双碳”政策的不断推行以及人们环保意识的不断提高,一氧化碳(CO)作为典型的大气污染物,已成为工业废气和汽车尾气排放的主要控制对象。贵金属型CO氧化催化剂具有优异的低温活性、抗中毒抗性能,是CO催化氧化处理的最为有效的手段之一。基于贵金属型CO氧化催化剂的研究现状,重点围绕Pt、Pd、Au、Ag、Rh贵金属催化剂的贵金属调控技术和载体可控技术进行综述,总结了贵金属型CO氧化催化剂的性能优化策略和发展方向,为开发高性能的CO氧化催化剂提供指导。

关键词：一氧化碳氧化贵金属催化剂低温催化负载技术

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渐开线圆柱齿轮的通用型滚插刀设计方法

工具技术 2024年第5期58卷 94-100页

作者：刘贝贝王时英辛佳毅太原理工大学机械与运载工程学院太原市030000 太原工具厂有限责任公司

针对渐开线圆柱齿轮的滚插加工,基于交错轴渐开螺旋面线接触啮合,提出一种通用型滚插刀设计方法,适用于任意结构前刀面。简述滚插加工原理,分析计算交错轴渐开螺旋面线接触啮合的条件;从渐开螺旋面出发,给出切削刃的计算方法,以重磨后... 详细信息

针对渐开线圆柱齿轮的滚插加工,基于交错轴渐开螺旋面线接触啮合,提出一种通用型滚插刀设计方法,适用于任意结构前刀面。简述滚插加工原理,分析计算交错轴渐开螺旋面线接触啮合的条件;从渐开螺旋面出发,给出切削刃的计算方法,以重磨后刃形精度不变为要求,构建后刀面模型;分析并给出了滚插加工参数的计算方法;基于滚插刀设计实例和VERICUT软件对不同参数的工件进行仿真加工,验证了所设计滚插刀的通用性与可行性,为通用型滚插刀设计提供了一种简单、无刃形误差的方法。

关键词：渐开线圆柱齿轮滚插刀切削刃加工参数仿真

高量秸秆不同深度还田对黑土有机质组成和酶活性的影响

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土壤学报 2015年第3期52卷 665-672页

作者：矫丽娜李志洪殷程程王晓飞辛士颖于磊吉林农业大学资源与环境学院长春130118 黑龙江省科学院大庆分院黑龙江大庆163319

在田间耕作条件下黑土3个土层（0—20、20。40、40～60cm）添加4％和8％高量玉米秸秆于尼龙袋中原位培养近4年后，研究不同层次土壤秸秆转化与有机碳积累特征，以及腐殖质各组分和土壤酶活性的变化。结果表明，添加14％的秸秆量0—20、2... 详细信息

在田间耕作条件下黑土3个土层（0—20、20。40、40～60cm）添加4％和8％高量玉米秸秆于尼龙袋中原位培养近4年后，研究不同层次土壤秸秆转化与有机碳积累特征，以及腐殖质各组分和土壤酶活性的变化。结果表明，添加14％的秸秆量0—20、20～40和40～60cm土层有机碳分别增加31．8％、96．4％和171．1％，8％秸秆添加量分别增加了86．2％、193．5％和265．9％，增加秸秆还田深度有利于土壤有机碳的积累。0～20cm土层在无秸秆还田情况下有机碳下降了29-3％，而20～40cm土层仅下降了1．8％。土壤有机碳含量和酶活性均随秸秆添加量的增加而提高，腐殖质胡敏酸／富里酸（HA／FA）比值发生较大变化，改善了腐殖质品质。各处理腐殖酸碳（HS—C）和胡敏酸碳（HA—C）的大小为20～40cm土层〉0～20cm土层〉40～60cm土层，而40～60cm土层则更有利于富里酸碳（FA—c）的积累。土壤过氧化氢酶活性、脲酶和蔗糖酶活性分别与HA—C、FA—C含量呈极显著正相关、显著正相关。研究结果为深层秸秆还田促进土壤有机质的积累提供了理论依据。

关键词：秸秆还田土层腐殖质积累特征土壤酶活性

基于TMT技术对高温胁迫条件下大菱鲆幼鱼心脏的差异蛋白筛选分析

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渔业科学进展 2024年第5期45卷 30-41页

作者：赵一诺徐荣静黄智慧蒋宇航曹郡文王新安刘诗颖闫鹏飞马爱军中国水产科学研究院黄海水产研究所、海水养殖生物育种与可持续产出全国重点实验室、山东省海洋渔业生物技术与遗传育种重点实验室、青岛市海水鱼类种子工程与生物技术重点实验室中国水产科学研究院黄海水产研究所、海水养殖生物育种与可持续产出全国重点实验室、山东省海洋渔业生物技术与遗传育种重点实验室、青岛市海水鱼类种子工程与生物技术重点实验室山东青岛266071 中国农业科学院研究生院北京100081 青岛海洋科技中心海洋生物学与生物技术功能实验室山东青岛266071 烟台开发区天源水产有限公司山东烟台264006

水温是影响鱼类生存生长的重要因素,而心脏对于维持脊椎动物机体稳态有关键作用。因此,为探究与大菱鲆(Scophthalmus maximus)耐高温性状相关的关键蛋白或代谢通路,本研究利用TMT联合LC-MS/MS技术开展高温胁迫下大菱鲆心脏差异表达蛋白... 详细信息

水温是影响鱼类生存生长的重要因素,而心脏对于维持脊椎动物机体稳态有关键作用。因此,为探究与大菱鲆(Scophthalmus maximus)耐高温性状相关的关键蛋白或代谢通路,本研究利用TMT联合LC-MS/MS技术开展高温胁迫下大菱鲆心脏差异表达蛋白谱分析。将大菱鲆幼鱼分为3组进行实验:control组(14.0±0.5)℃(适宜温度),HT1组(20.0±0.5)℃(高温),HT2组(28.0±0.5)℃(极高温)。定量分析鉴定到的肽段数量为35434个,其中特有肽段数量为31704个;鉴定到的蛋白质总数4765个。3个实验组间两两比较形成3个比较组,并进行差异蛋白筛选,结果显示,HT1/control共筛选到87个差异表达蛋白(DEPs),HT2/control共筛选到171个DEPs,HT2/HT1共筛选到188个DEPs。KEGG富集结果显示,HT2组与control组和HT2组与HT1组的差异蛋白均主要富集在与脂肪酸代谢、炎症以及免疫防御相关的通路;热休克蛋白家族中多个蛋白(HSP90B、HSP70等)在HT2组相较于control组均显著上调。蛋白互作网络结果显示,关联度最高的是HSP90B。首次发现,高温胁迫下心脏组织纤维蛋白原相关蛋白(FGA、FGB和FGG)表达水平呈上升趋势,其在热响应中的作用仍需进一步研究。结果表明,极高温可能引起大菱鲆的脂肪酸代谢紊乱,引发炎症反应,而热休克蛋白家族蛋白的上调可能对缓解热胁迫下的应激症状有积极作用。本研究为进一步阐明大菱鲆热应激的分子机制提供了理论依据,并为大菱鲆的耐高温选育提供候选蛋白标记。

关键词：大菱鲆蛋白组学高温胁迫心脏

血清IL-33和TNF-α活性及外周血淋巴细胞中NF-κB水平与冠心病不同程度病变的相关性分析

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蚌埠医学院学报 2024年第2期49卷 157-161页

作者：鲜维谭鑫耿嘉逸邹仕英杨海晨康品方张恒蚌埠医科大学第一附属医院心血管内科安徽蚌埠233004 蚌埠医科大学公共卫生学院安徽蚌埠233030 蚌埠医科大学心脑血管病研究中心安徽蚌埠233030

目的:分析冠心病(CHD)冠状动脉病变严重程度与血清白细胞介素33(IL-33)和肿瘤坏死因子α(TNF-α)活性水平及外周血淋巴细胞中核因子κB(NF-κB)蛋白表达之间的相关性,探讨其在CHD发展中的作用及价值。方法:选择就诊于心内科并行冠状动... 详细信息

目的:分析冠心病(CHD)冠状动脉病变严重程度与血清白细胞介素33(IL-33)和肿瘤坏死因子α(TNF-α)活性水平及外周血淋巴细胞中核因子κB(NF-κB)蛋白表达之间的相关性,探讨其在CHD发展中的作用及价值。方法:选择就诊于心内科并行冠状动脉造影检查并确诊为CHD的病人167例(CHD组),同时期行冠脉造影正常对照27例(CON组)。根据Gensini评分将167例CHD病人分成轻微病变组(<30分,LS组)84例、中度病变组(30~<60分,MS组)54例和重度病变组(≥60分,HS组)29例。实验室行ELISA检测血清中IL-33和TNF-α活性水平,分析在不同冠状动脉病变程度下IL-33和TNF-α的活性水平差异,探讨IL-33和TNF-α活性水平与CHD的关联性。同时取CHD组及CON组的外周血淋巴细胞,提取蛋白,使用Western blotting检测NF-κB蛋白表达情况。结果:HS组血清总胆固醇(TC)与CON组和LS组、MS组相比升高(P<0.05),HS组血清IL-33与TNF-α水平较MS组、LS组和对照组升高,并且随着冠状动脉病变程度的加重逐渐升高(P<0.01),两者之间呈正相关关系(r=0.663,P<0.01);CHD组中NF-κB蛋白表达低于CON组(P<0.01)。结论:随着冠状动脉病变程度的加重,血清IL-3和TNF-α水平也逐渐升高,且两者间存在一定程度的线性相关,同时与CON组相比,CHD病人NF-κB蛋白表达显著升高。

关键词：冠心病白细胞介素33 肿瘤坏死因子α 核因子-κB

A novel advanced oxidation process to degrade organic pollutants in wastewater:Microwave-activated persulfate oxidation

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Journal of Environmental Sciences 2009年第9期21卷 1175-1180页

作者： YANG shiying WANG Ping YANG xin WEI Guang ZHANG Wenyi SHAN Liang Key Laboratory of Marine Environment & Ecology Ministry of Education Qingdao 266100 China. College of Environmental Science and Engineering Ocean University of China Qingdao 266100 China. Qingdao Huashan Biochemical Company Limited Qingdao 266216 China

This article, for the first time, provides a novel advanced oxidation process based on sulfate radical （SO^4·-） to degrade organic pollutants in wastewater： microwave （MW）-activated persulfate oxidation （APO） with or without active carbon （AC）. Azo dye acid Orange 7 （AO7） is used as a model compound to investigate the high reactivity of MW-APO. It is found that AO7 （up to 1000 mg/L） is completely decolorized within 5-7 min under an 800 W MW furnace assisted-APO. In the presence of chloride ion （up to 0.50 mol/L）, the decolorization is still 100% completed, though delayed for about 1-2 min. Experiments are made to examine the enhancement by AC. It is exciting to find that the 100% decolorization of AO7 （500 mg/L） is achieved within 3 min by MW-APO using 1.0 g/L AC as catalyst, while the degradation efficiency maintains at 50% by MW energy without persulfate after about 5 min. Besides the destruction of visible light chromophore band of AO7 （484 nm）, during MW-APO, two bands in the ultraviolet region （228 nm and 310 nm） are rapidly broken down. The removal of COD is about 83%-95% for 500 mg/L AO7. SO^4·- is identified with quenching studies using specific alcohols. Both SO^4·- and ·OH could degrade AO7, but SO^4·- plays the dominant role. In a word, MW-APO AC is a new catalytic combustion technology for destruction of organic contamination even for high concentration.

关键词： microwave-activated persulfate oxidation active carbon sulfate radical advanced oxidation technology

The Genetics of Clinical Liver Diseases: Insight into the TM6SF2 E167K Variant

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Journal of Clinical and Translational Hepatology 2018年第3期6卷 326-331页

作者： Xiaoyu Zhang Shousheng Liu Quanjiang Dong Yongning xin shiying Xuan Department of Gastroenterology Taishan Medical UniversityTaianChina Department of Infectious Disease Qingdao Municipal HospitalQingdaoChina Department of Gastroenterology Qingdao Municipal HospitalQingdaoChina Digestive Disease Key Laboratory of Qingdao QingdaoChina Central Laboratories Qingdao Municipal HospitalQingdaoChina

The transmembrane 6 superfamily member 2(TM6SF2)gene E167K variant(rs58542926)was identified by exome-wide as-sociation study as a nonsynonymous single nucleotide poly-morphism associated with nonalcoholic fatty liver *** TM6SF2 E167K variant features a C-to-T substitution at nucleotide 499,encoding a glutamate with lysine change at codon 167(E167K).TM6SF2 is markedly expressed in the liver,small intestine and kidney,and has been proposed as an im-portant risk factor for diseases associated with lipid ***,multifunctional studies of the TM6SF2 E167K variant have been carried out in a spectrum of liver dis-eases,such as nonalcoholic fatty liver disease,nonalcoholic steatohepatitis,fibrosis,cirrhosis,and viral *** re-view summarizes the research status of the TM6SF2 E167K variant in different liver diseases and specific populations,and discusses the potential mechanisms of the TM6SF2 E167K var-iant's role in the progression of various liver diseases.

关键词： TM6SF2 SNP NAFLD Fibrosis Cirrhosis Virus hepatitis

Tenofovir Alafenamide Fumarate,Tenofovir Disoproxil Fumarate and Entecavir:Which is the Most Effective Drug for Chronic Hepatitis B?A Systematic Review and Meta-analysis

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Journal of Clinical and Translational Hepatology 2021年第3期9卷 335-344页

作者： Xuefeng Ma Shousheng Liu Mengke Wang Yifen Wang Shuixian Du Yongning xin shiying Xuan Department of Infectious Disease Qingdao Municipal HospitalQingdao UniversityQingdaoShandongChina Clinical Research Center Qingdao Municipal HospitalQingdao UniversityQingdaoShandongChina Digestive Disease Key Laboratory of Qingdao QingdaoShandongChina

Background and Aims:The therapeutic effect of tenofovir alafenamide fumarate(TAF),tenofovir disoproxil fumarate(TDF)and entecavir(ETV)on chronic hepatitis B(CHB)patients remains *** aim of this study was to explore the differences in virological responses to TAF,TDF and ETV in patients with ***:Literature searches were conducted of the PubMed,EMBASE,and the Cochrane Library databases to identify randomized controlled trials and observational studies published up to July 21,*** comparisons of virological response between TDF,ETV,and TAF were carried out with pooled odds ratio(OR)***:The virological response in TDF-treated CHB patients was notably superior to that of the ETV-treated CHB patients after 12-weeks[OR=1.12,95%confidence interval(CI):0.89–1.41],24-weeks(OR=1.33,95%CI:1.11–1.61),48-weeks(OR=1.62,95%CI:1.16–2.25),72-weeks(OR=1.43,95%CI:0.78–2.62),and 96-weeks(OR=1.56,95%CI:0.87–2.81)*** significant difference was observed for the virological responses in CHB patients after 48-weeks treatment with TAF or *** virological response in TDF+ETV-treated CHB patients was superior to that of TDF-treated CHB patients after 24-weeks,48-weeks(OR=1.54,95%CI:1.17–2.02),96-weeks,and ***:The virological response in TDF-treated CHB patients was superior to that in ETV-treated CHB patients,but there was no significant difference between TAF and *** addition,the therapeutic effect of TDF+ETV was superior to TDF alone.

关键词： Tenofovir alafenamide Entecavir Tenofovir disoprox Chronic hepatitis B Virological response