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Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush

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Chinese Journal of Polymer Science 2018年第4期36卷 472-478页

作者： Jie Zhao Ru Mo Li-Mei Tian Ling-Jie Song shi-fang luan Jing-Hua Yin Lu-Quan Ren Key Laboratory of Bionic Engineering Ministry ofEdueationJilin UniversityChangchun 130022China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Jilin Province People's Hospital Changchun 130021China

High sensitive immunoassay platforms have gained intense attention due to their vital roles in early-stage disease diagnosis and therapeutic information feedback. Although random covalent-binding of antibody has been widely adopted in immunoassays due to its simplicity and effectiveness, it readily loses its activity and fails to exhibit high antigen-binding capacity. In this work, copolymer of zwitterionic sulfobetaine methacrylate（SBMA） and glycidyl methacrylate（GMA） brushes were immobilized onto inert polypropylene（PP） via surface-initiated atom transfer radical polymerization（ATRP） based on biomimetic dopamine pretreatment. Subsequently, boronic acid（BA） groups were covalently bonded via active GMA units, followed by the introduction of oriented immobilization of antibody. Owing to the oriented immobilization of antibody facilitated by BA groups in polymer brush, the bioactivity of antibody is well preserved, which endows the surface with significantly enhanced antigen-binding capacity. Moreover, the existence of SBMA segments in polymer brushes renders the surface high resistance to nonspecific protein adsorption, significantly alleviating the signal interference of antigen recognition. This strategy could find potential applications in developing high sensitive immunoassay platforms based on the different substrates.

关键词： Biomimetic dopamine Atom transfer radical polymerization (ATRP) Boronic acid (BA) Immunoassay Biomimetic

A Biomimetic Surface for Infection-resistance through Assembly of Metal-phenolic Networks

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Chinese Journal of Polymer Science 2018年第5期36卷 576-583页

作者： Ru-Jian Jiang Shun-Jie Yan Li-Mei Tian shi-Ai Xu Zhi-Rong Xin shi-fang luan Jing-Hua Yin Lu-Quan Ren Jie Zhao Key Laboratory of Bionic Engineering Ministry of Education Jilin University School of Chemistry and Chemical Engineering Yantai University State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences

Despite the fact that numerous infection-resistant surfaces have been developed to prevent bacterial colonization and biofilm formation, developing a stable, highly antibacterial and easily produced surface remains a technical challenge. As a crucial structural component of biofilm, extracellular DNA（eDNA） can facilitate initial bacterial adhesion, subsequent development, and final maturation. Inspired by the mechanistic pathways of natural enzymes（deoxyribonuclease）, here we report a novel antibacterial surface by employing cerium（Ce（Ⅳ）） ion to mimic theDNA-cleavage ability of natural enzymes. In this process, the coordination chemistry of plant polyphenols and metal ions was exploited to create an in situ metal-phenolic film on substrate surfaces. Tannic acid（TA） works as an essential scaffold and Ce（Ⅳ） ion acts as both a cross-linker and a destructor of eDNA. The Ce（Ⅳ）-TA modified surface exhibited highly enhanced bacteria repellency and biofilm inhibition when compared with those of pristine or Fe（Ⅲ）-TA modified samples. Moreover, the easily produced coatings showed high stability under physiological conditions and had nontoxicity to cells for prolonged periods of time. This as-prepared DNA-cleavage surface presents versatile and promising performances to combat biomaterial-associated infections.

关键词： Antibacterial surface Metal-phenolic coating DNA-cleavage Biomimetic surface

生物医用材料表面光引发活性接枝聚合研究新进展

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高分子学报 2021年第3期52卷 223-234页

作者：孙立伟宋凌杰栾世方殷敬华中国科学院长春应用化学研究所高分子物理与化学国家重点实验室长春130022 中国科学技术大学应用化学与工程学院合肥230026 威高集团有限公司医用植入器械国家工程实验室威海264210

表面接枝聚合改性已经成为提升生物医用材料性能的最重要方法之一.参比其他活性接枝聚合方法,光引发活性接枝聚合因其独特的优势已被越来越广泛地应用于生物医用材料表面改性.根据光引发剂的类型,目前应用最多的光引发活性接枝聚合的引... 详细信息

表面接枝聚合改性已经成为提升生物医用材料性能的最重要方法之一.参比其他活性接枝聚合方法,光引发活性接枝聚合因其独特的优势已被越来越广泛地应用于生物医用材料表面改性.根据光引发剂的类型,目前应用最多的光引发活性接枝聚合的引发体系主要有3种:光引发-转移-终止剂介导的聚合引发体系、二苯甲酮及其衍生物引发体系、硫杂蒽酮类引发体系.本文首先简要介绍了上述3种光引发活性接枝聚合体系的发展历程、接枝机理以及特点.同时结合本课题组相关研究工作,重点论述了光引发接枝聚合技术在3个不同生物医用领域的主要应用:(1)抗菌表面,利用光活性接枝的特点构建层状功能高分子刷,实现表面抗菌功能的阶段性需求.(2)免疫检测表面,使用光活性接枝方法构建层状功能高分子刷,解决检测灵敏度低以及蛋白干扰问题.(3)生物活性分子表面固定,利用可见光活性接枝聚合体系,实现酶在表面的固定化使用以及细胞表面修饰以提高细胞稳定性.最后展望了生物医用材料表面光引发活性接枝聚合研究的发展趋势.

关键词：光活性接枝聚合表面改性抗菌免疫检测生物活性分子

Interaction of major facilitator superfamily domain containing 2A with the blood-brain barrier

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Neural Regeneration Research 2025年第8期20卷 2133-2152页

作者： Yilun Ma Taiwei Dong Fei luan Juanjuan Yang Feng Miao Peifeng Wei College of Pharmacy and First Clinical Medical College Shaanxi University of Chinese MedicineXianyangShaanxi ProvinceChina National Drug Clinical Trial Agency The Second Affiliated Hospital of Shaanxi University of Chinese Medicine/Xixian New District Central HospitalXi′anShaanxi ProvinceChina

The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the blood-brain barrier remain poorly *** major facilitator superfamily domain containing 2A has been identified as a key regulator of blood-brain barrier *** plays a critical role in promoting and maintaining the formation and functional stability of the blood-brain barrier,in addition to the transport of lipids,such as docosahexaenoic acid,across the blood-brain ***,an increasing number of studies have suggested that major facilitator superfamily domain containing 2A is involved in the molecular mechanisms of blood-brain barrier dysfunction in a variety of neurological diseases;however,little is known regarding the mechanisms by which major facilitator superfamily domain containing 2A affects the blood-brain *** paper provides a comprehensive and systematic review of the close relationship between major facilitator superfamily domain containing 2A proteins and the blood-brain barrier,including their basic structures and functions,cross-linking between major facilitator superfamily domain containing 2A and the blood-brain barrier,and the in-depth studies on lipid transport and the regulation of blood-brain barrier *** comprehensive systematic review contributes to an in-depth understanding of the important role of major facilitator superfamily domain containing 2A proteins in maintaining the structure and function of the blood-brain barrier and the research progress to *** will not only help to elucidate the pathogenesis of neurological diseases,improve the accuracy of laboratory diagnosis,and optimize clinical treatment strategies,but it may also play an important role in prognostic *** addition,the effects of major facilitator superfamily domain containing 2A on blood-brain barrier lea

关键词： blood-brain barrier(BBB) caveolin-1 central nervous system docosahexaenoic acid endothelial cells lysophosphatidylcholine major facilitator superfamily domain containing 2A(MFSD2A) transcytosis

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

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Neural Regeneration Research 2025年第3期20卷 900-912页

作者： Baoqi Hu Rui Wang Hanyue Zhang Xiou Wang Sijia Zhou Bo Ma Yan luan Xin Wang Xinlin Chen Zhichao Zhang Qianyan Kang Department of Ophthalmology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Northwest UniversityXi’anShaanxi ProvinceChina Institute of Neurobiology Xi’an Jiaotong University Health Science CenterXi’anShaanxi ProvinceChina

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell ***,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development ***,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo *** this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in ***,we used SOX2 and PAX6 immunostaining to identify retinal progenitor *** then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,***-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between ***,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,*** timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant *** study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina *** findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

关键词： bipolar cells differentiation in vivo microglia Müller glia organotypic retinal explant culture postnatal retina development proliferation retinal progenitor cells

Encapsulating perovskite quantum dots into 3D COF for visible light-driven CO2 reduction

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Science China Chemistry 2025年

作者： Xinxin Wang Yucheng Jin Xiya Yang Qingyu luan Tianyu Wang Dongdong Qi Kang Wang Jianzhuang Jiang College of Chemistry and Materials Science Anhui Normal University Beijing Advanced Innovation Center for Materials Genome Engineering Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials Department of Chemistry and Chemical Engineering School of Chemistry and Biological EngineeringUniversity of Science and Technology Beijing

Lead halide perovskite quantum dots（LHP QDs） have been revealed to possess great potential in photocatalytic applications including CO2reduction, which however suffer from poor stability. Herein, a high crystalline hydrazine-linked three-dimensional（3D） covalent organic framework, USTB-17, was fabricated from the reaction between 12-connected building block and 4-connected 3,5,7-tetrakis（4-aldophenyl）-adamantane. Post-modification with Ni2+affords the metallic framework USTB-17（Ni）followed by sequential deposition of the CH3NH2PbI3（MAPbI3） perovskite QDs into its pores, generating the USTB-17（Ni）@MAPbI3composite. Powder X-ray diffraction analysis together with theoretical simulations and transmission electron microscopy discloses the crystalline nature of USTB-17, USTB-17（Ni）, and USTB-17（Ni）@MAPbI3with an unprecedented noninterpenetrated hpt topology. The close contact of QDs inside the COF pores with the Ni catalytic site locating at the pore surface of COF allows a rapid transfer of the photogenerated electrons in QDs to the Ni catalytic sites, enhancing the photocatalytic activity for CO2reduction. This endows USTB-17（Ni）@MAPbI3with efficient photocatalysis performance for photocatalytic CO2reduction with CO generation rate of 365 μmol g-1h-1and CO selectivity up to 96% under visible-light irradiation, 7 times higher than that of USTB-17（Ni）. After four cycles of reactions, the photocatalytic CO generation rate remains almost unchanged, demonstrating its excellent cycle stability.

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QTL-seq identifies major quantitative trait loci of stigma color in melon

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Horticultural Plant Journal 2021年第4期7卷 318-326页

作者： Aohan Qiao Xufeng fang shi Liu Hongyu Liu Peng Gao Feishi luan Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural AffairsNortheast Agricultural UniversityHarbin 150030China College of Horticulture and Landscape Architecture Northeast Agricultural UniversityHarbin 150030China

Stigma color plays an important role in *** nature,melon(Cucumis melo L.)stigmas are either yellow or green;however,a review of the literature found no report on how stigma color affects pollination and fruit development in ***,we used an F_(2)melon population derived from a cross between‘MR-1’(P_(1),with green stigmas)and‘M1–32’(P_(2),with yellow stigmas),and performed genetic analysis and *** results of bulked segregant analysis allowed the identification of genetic loci controlling stigma color on chromosomes 6 and *** F2 population consisting of 150 individuals was used for initial mapping.A genetic map of 304.17 cM was constructed using 37 cleaved amplified polymorphism sequence(CAPS)*** identified one major quantitative trait locus(QTL)and one minor QTL for stigma *** major QTL GS8.1 was further mapped to a 4.13 cM interval between CAPS markers 8C-10 and 8C-16,which explained 27.04%of the phenotypic *** addition,GS6.1 was mapped between E-49 and 6A-7,explaining 18.6%of the phenotypic *** study provides a theoretical basis for the fine mapping and cloning of melon genes controlling stigma color.

关键词： Cucumis melo L. Stigma color BSA QTL chlorophyll content CAPS

Periodic Solutions of Nonautonomous Second Order Hamiltonian Systems

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Acta Mathematica Sinica,English Series 2005年第4期21卷 685-690页

作者： shi Xia luan An Min MAO Department of Mathematics Qufu Normal University Qufu 273165. P. R. China Institute of Mathematics Academy of Mathematics and Systems Science Chinese Academy of Sciences. Beijing 100080. P. R. China

In this paper, we develop the local linking theorem given by Li and Willein by replacing the Palais-Smale condition with a Cerami one, and apply it to the study of the existence of periodic solutions of the nonautonomous second order Hamiltonian systems （H） ü＋A（t）u＋∨V（t, u）=0, u∈R^N, t∈R. We handle the case of superquadratic nonlinearities which differ from those used previously. Our results extend the theorems given by Li and Willem.

关键词： Non-autonomous Hamiltonian systems Periodic solutions Local linking Critical point theory Variational method

Catalytic Characteristics of Lipase Immobilized in CTAB/TX-100 MBG

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Chemical Research in Chinese Universities 2010年第1期26卷 110-113页

作者： SONG Shao-fang luan Yu-xia College of Chemistry and Material Science Shandong Agricultural University Tai ＇an 271018 P. R. China Institute of Pharmaceuties School of Pharmaceutical Science Shandong University Jinan 250100 P. R. China

CTAB/TX-100 microemtdsion-based gel（MBG）, which could be soaked in aqueous solution for a long time without mechanical strength and shape changes, was successfully prepared. Lipase immobilized in this gel had a higher activity than that in CTAB MBG when catalyzed the esterification reaction between n-hexanoic acid and n-octanol and its enantioselectivity was slightly higher when catalyzed the stereoselective esterification reaction between racemic ibuprofen and n-octanol. In aqueous solution, lipase in CTAB/TX-100 MBG could catalyze smoothly the hydrolysis reaction of olive oil and could be reused many times without the evident change of MBG mechanical strength although its activity decreased 41.8% after nine-time recycles.

关键词： Microemulsion-based gel(MBG) Immobilized lipase Activity Stereoselectivity Esterification Hydrolysis