Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush

作     者：Jie Zhao Ru Mo Li-Mei Tian Ling-Jie Song Shi-Fang Luan Jing-Hua Yin Lu-Quan Ren 

作者机构：Key Laboratory of Bionic EngineeringMinistry ofEdueationJilin UniversityChangchun 130022China State Key Laboratory of Polymer Physics and ChemistryChangchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Jilin Province People's HospitalChangchun 130021China 

出 版 物：《Chinese Journal of Polymer Science》 (高分子科学（英文版）)

年 卷 期：2018年第36卷第4期

页      面：472-478页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 08[工学] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 100102[医学-免疫学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 10[医学] 

基　　金：financially supported by the Research Program Funds of Jilin University(Nos.419080500665 and 451170301076) 

主　　题：Biomimetic dopamine Atom transfer radical polymerization (ATRP) Boronic acid (BA) Immunoassay Biomimetic 

摘      要：High sensitive immunoassay platforms have gained intense attention due to their vital roles in early-stage disease diagnosis and therapeutic information feedback. Although random covalent-binding of antibody has been widely adopted in immunoassays due to its simplicity and effectiveness, it readily loses its activity and fails to exhibit high antigen-binding capacity. In this work, copolymer of zwitterionic sulfobetaine methacrylate（SBMA） and glycidyl methacrylate（GMA） brushes were immobilized onto inert polypropylene（PP） via surface-initiated atom transfer radical polymerization（ATRP） based on biomimetic dopamine pretreatment. Subsequently, boronic acid（BA） groups were covalently bonded via active GMA units, followed by the introduction of oriented immobilization of antibody. Owing to the oriented immobilization of antibody facilitated by BA groups in polymer brush, the bioactivity of antibody is well preserved, which endows the surface with significantly enhanced antigen-binding capacity. Moreover, the existence of SBMA segments in polymer brushes renders the surface high resistance to nonspecific protein adsorption, significantly alleviating the signal interference of antigen recognition. This strategy could find potential applications in developing high sensitive immunoassay platforms based on the different substrates.