检索结果-南通市图书馆

Transcriptome analysis and metabolic profiling reveal the key role of carotenoids in the petal coloration of Liriodendron tulipifera

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2020年第1期7卷 1955-1970页

作者： Zhaodong Hao Siqin Liu Lingfeng Hu jisen shi Jinhui Chen Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjingJiangsuChina

Liriodendron tulipifera,also known as tuliptree,is a popular ornamental horticultural plant with extraordinary tulipshaped flowers characterized by an orange band near their *** mechanisms underlying petal band-specific pigmentation during *** flower development are ***,we combined nontargeted and targeted metabolomics and transcriptomics to identify a pathway cascade leading to carotenoid biosynthesis that is specifically activated in the petal *** comparative analysis of carotenoid metabolites between *** and Liriodendron hybrids indicates thatγ-carotene,a rare carotene in plants,is the most likely orange pigment responsible for the coloration of the petal *** and transcriptomic analyses of developing petals reveal that the band area is first predefined by the loss of green ***,the band is maintained by locally activating and repressing carotenoid and chlorophyll biosynthesis genes,*** rate-limiting genes of carotene biosynthesis,carotenoid isomerase(CRTISO)and epsilon lycopene cyclase(ε-LCY),encode the core enzymes responsible for petal band-specific orange pigmentation in *** particular,a putative additionalε-LCY copy specific to *** may contribute to the distinct petal coloration pattern,compared with *** together,our work provides a first glimpse of the metabolome and transcriptome dynamics in tuliptree flower coloration and provides a valuable resource for flower breeding or metabolic engineering as well as for understanding flower evolution in an early woody angiosperm.

关键词： orange analysis flowers

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2013年第1期56卷 82-89页

作者： XIA Bing DONG Chen ZHANG WenYi LU Ye CHEN JinHui shi jisen Key Laboratory of Forest Genetics & Biotechnology (Ministry of Education of China) Nanjing Forestry University Advanced Analysis and Testing Center Nanjing Forestry University

The characteristics of the interactions co-cultures of ultrafine mesoporous silica nanoparticles （MSNs） and the Liriodendron hybrid suspension cells were systematically investigated using laser scanning confocal microscope （LSCM） and scanning elec- tron microscopy （SEM）. Using fluorescein isothiocyanate （FITC） labeling, the LSCM observations demonstrated that MSNs （size, 5-15 nm） with attached FITC molecules efficiently penetrated walled plant cells through endocytic pathways, but free FITC could not enter the intact plant cells, The SEM measurements indicated that MSNs readily aggregated on the surface of intact plant cells, and also directly confirmed that MSNs could enter intact plant cells; this was achieved by determining the amount of silicon present. After 24 h of incubation with 1.0 mg mL-t of MSNs, the viability of the plant cells was analyzed using fluorescein diacetate staining; the results showed that these cells retained high viability, and no cell death was observed. Interestingly, after the incubation with MSNs, the Liriodendron hybrid suspension cells retained the capability for plant regen- eration via somatic embryogenesis. Our results indicate that ultrafine MSNs hold considerable potential as nano-carriers of ex- tracellular molecules, and can be used to investigate in vitro gene-delivery in plant cells.

关键词： mesoporous silica nanoparticles endocytosis cytotoxicity somatic embryogenesis

Efficient Evergreen Plant Regeneration of Cinnamomum japonicum *** in vitro Organogenesis

在线全文

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2021年第2期90卷 571-582页

作者： Jiaji Zhang Xiaofei Long Yuhao Weng Tielong Cheng jisen shi Jinhui Chen Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing210037China College of Biology and the Environment Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing210037China

Cinnamomum japonicum *** an excellent roadside tree and medicinal tree species with considerable ornamental and economic *** this study,we successfully developed a large-scale micropropagation protocol for *** for the first *** shoots were excised and used as explants for shoot induction on several basal media,supplemented with different concentrations of plant growth regulators(PGRs),such as Thidiazuron(TDZ),N^(6)-Benzyladenine(6-benzylaminopurine)(BA),α-naphthaleneacetic acid(NAA)and Gibberellic acid(GA_(3)).After comparison,the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog(MS)medium containing 0.5 mg L^(-1)BA,0.05 mg L^(-1)NAA and 0.2 mg L^(-1)GA_(3),which resulted in an average number of induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d,***,elongated adventitious shoots were transferred to induce roots.86.7%of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg L^(-1)NAA and 0.1 mg L^(-1)*** earliest rooting time observed was after 21 d and the average root length was up to 3.3 cm after 28 *** study shows that *** can be successfully regenerated through de novo organogenesis,which lays a foundation for future transformation research on this tree.

关键词： Cinnamomum japonicum Sieb. micropropagation de novo organogenesis shoot induction rhizogenesis

Spatial analysis increases efficiency of progeny testing of Chinese fir

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Forestry Research 2017年第3期28卷 445-452页

作者： Liming Bian Renhua Zheng Shunde Su Huazhong Lin Hui Xiao Harry Xiaming Wu jisen shi South China Forestry Multidisciplinary Collaborative Innovation Center College of ForestryNanjing Forestry University159 Longpan RoadNanjing 210037People'sRepublic of China Fujian Academy of Forestry 35 ShangchiqiaoFuzhou 350012People's Republic of China Jiangle Forest Farm Jiangle 353300People's Republic of China Department of Forest Genetics and Plant Physiology Umea Plant Science CentreSwedish University of Agricultural Sciences90183 UmeaSweden College of Forestry Nanjing Forestry University159 Longpan RoadNanjing 210037People's Republic of China

We used spatial, global trend and post-blocking analysis to examine the effectiveness of a progeny trial in a tree breeding program for Chinese fir （Cunninghamia lanceolata （Lamb.） Hook） on a hilly site with an environmental gradient from hill top to bottom. Diameter at breast height （DBH） and tree height data had significant spatial auto-correlations among rows and columns. Adding a firstorder separable autoregressive term more effectively modelled the spatial variation than did the incomplete block （IB） model used for the experimental design. The spatial model also accounted for effects of experimental design factors and greatly reduced residual variances. The spatial analysis rel- ative to the IB analysis improved estimation of genetic parameters with the residual variance reduced 13 and 19% for DBH and tree height, respectively; heritability increased 35 and 51% for DBH and tree height, respectively; and genetic gain improved 3-5%. Fitting global trend and postblocking did not improve the analyses under IB model. The use of a spatial model or combined with a design model is recommended for forest genetic trials, particularly with global trend and local spatial variation of hilly sites.

关键词： Chinese fir Genetic variance Heritabilities Progeny testing Spatial analysis

Inheritance of growth and survival in two 9-year-old, open-pollinated progenies of an advanced breeding population of Chinese firs in southeastern China

在线全文

学校读者我要写书评

暂无评论

Journal of Forestry Research 2016年第5期27卷 1067-1075页

作者： Renhua Zheng Zhou Hong Shunde Su Liming Bian Hui Xiao jisen shi Harry X. Wu Fujian Academy of Forestry Fuzhou 350012 People's Republic of China The Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China Fuzhou 350012 People's Republic of China State Forestry Administration Engineering Research Center of Chinese Fir Fuzhou 350012 People's Republic of China The Key Laboratory of Forest Culture and Forest Product Processing Utilization of Fujian Province Fuzhou 350012People's Republic of China Research Institute of Tropical Forestry Chinese Academy of Forestry No. 682 Guangshan 1 Rd. Guangzhou 510520People's Republic of China The Key Laboratory of Forest Genetics and Biotechnology Nanjing Forestry University Nanjing 210037 People's Republic of China Department of Forest Genetics and Plant Physiology Umea Plant Science Centre Swedish University of Agricultural Sciences 90183 Umea Sweden CSIRO NRCA Black Mountain GPO Box 1600 CanberraACT 2601 Australia

Tree growth traits （tree height, DBH and stem volume） and survival from two 9-year-old, open-pollinated progeny tests of Chinese fir were investigated for heri- tability, genotype × environment interaction, age-age genetic correlation and selection efficiency. The 97 and 79 families planted at two sites were collected from the thirdcycle seed orchard. Individual heritability was estimated between 0.05 and 0.21 for tree height, DBH, and volume and between 0.45 and 1.0 for survival. Family heritability was between 0.20 and 1.14. Significant genotype x envi- ronment interaction was observed for the three growth traits. Type B genetic correlation was between 0.41 and 0.67 with an increasing trend as tree grows. High age-age genetic correlation was observed with correlation reaching 0.9 after age 4 for height, DBH, and volume. The genetic gains were estimated at 3.26, 3.39 and 5.98 % for tree height, DBH, and volume with 10 % selection intensity. The implication for advanced tree breeding in Chinese fir is discussed.

关键词： Chinese fir - Progeny test Heritability -Genetic gain

Morphological, phenological, and transcriptional analyses provide in sight into the diverse flowering traits of a mutant of the relic woody plant Liriodendron chinense

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2021年第1期8卷 2345-2360页

作者： Yu Sheng Zhaodong Hao Ye Peng Siqin Liu Lingfeng Hu Yongbao Shen jisen shi Jinhui Chen Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjingChina College of Biology and the Environment Nanjing Forestry UniversityNanjingChina Southern Tree Seed Inspection Center National Forestry Administration Nanjing Forestry UniversityNanjingChina

Flowering is crucial to plant reproduction and controlled by multiple ***,the mechanisms underlying the regulation of flowering in perennial plants are still largely ***,we first report a super long blooming 1(slbl)mutant of the relict tree Liriodendron chinense possessing a prolonged blooming period of more than 5 months,in contrast to the 1 month blooming period in the wild type(WT).Phenotypic characterization showed that earlier maturation of lateral shoots was caused by accelerated axillary bud fate,leading to the phenotype of continuous flowering in slbl *** transcriptional activity of genes related to hormone signaling(auxin,cytokinin,and strigolactone),nutrient availability,and oxidative stress relief further indicated active outgrowth of lateral buds in slbl ***,we discovered a unique FT splicing variant with intron retention specific to slbl mutants,representing a potential causal mutation in the slbl ***,most slbl inbred offspring flowered precociously with shorter juvenility(~4 months)than that(usually 8-10 years)required in WT plants,indicating heritable variation underlying continuous flowering in slbl *** study reports an example of a perennial tree mutant that flowers continuously,providing a rare resource for both breeding and genetic research.

关键词： traits Morphological flowers

Overexpression of the LcPIN2 and LtPIN2 Gene in Arabidopsis thaliana Promotes Root Elongation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2023年第8期92卷 2383-2397页

作者： Zijian Cao Guoxia Xue Lingfeng Hu Haoxian Qu Shuang Liang jisen shi Jinhui Chen Zhaodong Hao Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing210037China

The auxin polar transporter,PIN-FORMED 2(PIN2)plays an important role in root ***,it remains unclear whether PIN2 genes form two Liriodendron species,***(LcPIN2)and ***(LtPIN2),are both involved in root development and whether and to what extent these two genes diverge in ***,we cloned and overexpressed LcPIN2 and LtPIN2 in Arabidopsis thaliana wild-type(WT)and Atpin2 *** and sequence analysis showed a small degree of differentiation between these two Liriodendron PIN2 ***-speciﬁc gene expression analysis indicated that both Liriodendron PIN2 genes were highly expressed in roots,implying a potential role in root ***,heterologous overexpression of LcPIN2 and LtPIN2 in Arabidopsis both signiﬁcantly increased the root length compared to wild-type and empty ***,the root length defect in Atpin2 was complemented both by LcPIN2 and ***,heterologous overexpression of LcPIN2 and LtPIN2 cannot rescue the defect in root gravitropism of Atpin2 *** together,ourﬁndings unravel PIN2 genes from the magnoliids plant Liriodendron were functionally conserved with AtPIN2 in the dicotyledonous plant Arabidopsis in regard to the regulation of root length,but not root *** study also provides a potential target for genetic improvement of the root system in these valuable forest trees Liriodendron.

关键词： Liriodendron PIN2 auxin polar transport root development

Genome-wide identification and cold stress-induced expression analysis of the CBF gene family in Liriodendron chinense

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Forestry Research 2021年第6期32卷 2531-2543页

作者： Yuanlin Guan Siqin Liu Weihuang Wu Kaiyue Hong Rongxue Li Liming Zhu Yang Liu Ye Lu Jinhui Chen Liming Yang jisen shi Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education of China Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing 210037JiangsuPeople’s Republic of China College of Biology and the Environment Nanjing Forestry UniversityNanjing 210037JiangsuPeople’s Republic of China

Cold-resistance pathways that operate in model plants such as Arabidopsis thaliana and Oryza sativa have been studied *** has been found that CBF genes play an important role in plant cold *** chinense,a tree known for its graceful tree shape and widely spread in south China,has weak cold ***,little is known about its response to *** further study the function of *** CBF gene family,we started by characterizing all members of this gene family in the *** genome and their expression *** analysis found that 14 CBF genes in *** are more closely related to their homologues in woody plants and *** than those in ***-acting elements and GO analysis showed that some LcCBF genes participated in the biological process of cold stress *** transcriptomic and RT-qPCR data showed that most of LcCBF genes displayed an initially increasing and subsequently decreasing trend during cold stress course and the expression profile of each member was *** LcCBF genes exhibited a different abundance in callus,root,stem and leaf *** structure and expression characteristics of LcCBF genes imply that they may have similar and different functions in response to cold stress *** identification and analysis of LcCBF gene family have laid the foundation for future studies into *** cold stress mechanisms and for the cultivation of cold-resistance cultivars.

关键词： Liriodendron chinense CBF genes Phylogenetic relation Expression profile Cold stress

CRISPR:从“盲盒”基因编辑到“精准靶向”基因组编辑的未竟之旅

在线全文

学校读者我要写书评

暂无评论

南京林业大学学报（自然科学版） 2021年第6期45卷 12-14页

作者：施季森南京林业大学林木遗传与生物技术教育部重点实验室南京林业大学林学院南方现代林业协同创新中心江苏南京210037

《CRISPR/Cas基因组编辑》因获2020年诺贝尔化学奖而再度引起广泛关注。自1970年报道基因编辑小鼠以来,约有33 850条文献涉及基因编辑(据ScienceDirect)。2012年CRISPR/Cas9技术突破后的文献占98.32%,其中涉农、林植物基因(组)编辑文献... 详细信息

《CRISPR/Cas基因组编辑》因获2020年诺贝尔化学奖而再度引起广泛关注。自1970年报道基因编辑小鼠以来,约有33 850条文献涉及基因编辑(据ScienceDirect)。2012年CRISPR/Cas9技术突破后的文献占98.32%,其中涉农、林植物基因(组)编辑文献占10.63%,林木CRISPR/Cas9基因组编辑研究也迅速起步。应编辑部之约,特付短评一二,供参考。

关键词：基因组编辑基因编辑植物基因报道基因诺贝尔化学奖