Efficient Evergreen Plant Regeneration of Cinnamomum japonicum *** in vitro Organogenesis

作     者：Jiaji Zhang Xiaofei Long Yuhao Weng Tielong Cheng Jisen Shi Jinhui Chen 

作者机构：Key Laboratory of Forest Genetics&Biotechnology of Ministry of EducationCo-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing210037China College of Biology and the EnvironmentCo-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing210037China 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)

年 卷 期：2021年第90卷第2期

页      面：571-582页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：This research is supported by Key research and development plan of Jiangsu Province(BE2017376) Foundation of Jiangsu forestry bureau(LYKJ42) the Qinglan project of Jiangsu province and Priority Academic Program Development of Jiangsu Higher Education Institutions to J.H.Chen,and the Nature Science Foundation of China(31770715)to T.L.Cheng 

主　　题：Cinnamomum japonicum Sieb. micropropagation de novo organogenesis shoot induction rhizogenesis 

摘      要：Cinnamomum japonicum *** an excellent roadside tree and medicinal tree species with considerable ornamental and economic *** this study,we successfully developed a large-scale micropropagation protocol for *** for the first *** shoots were excised and used as explants for shoot induction on several basal media,supplemented with different concentrations of plant growth regulators(PGRs),such as Thidiazuron(TDZ),N^(6)-Benzyladenine(6-benzylaminopurine)(BA),α-naphthaleneacetic acid(NAA)and Gibberellic acid(GA_(3)).After comparison,the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog(MS)medium containing 0.5 mg L^(-1)BA,0.05 mg L^(-1)NAA and 0.2 mg L^(-1)GA_(3),which resulted in an average number of induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d,***,elongated adventitious shoots were transferred to induce roots.86.7%of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg L^(-1)NAA and 0.1 mg L^(-1)*** earliest rooting time observed was after 21 d and the average root length was up to 3.3 cm after 28 *** study shows that *** can be successfully regenerated through de novo organogenesis,which lays a foundation for future transformation research on this tree.