Overexpression of the LcPIN2 and LtPIN2 Gene in Arabidopsis thaliana Promotes Root Elongation

作     者：Zijian Cao Guoxia Xue Lingfeng Hu Haoxian Qu Shuang Liang Jisen Shi Jinhui Chen Zhaodong Hao 

作者机构：Key Laboratory of Forest Genetics&Biotechnology of Ministry of EducationCo-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing210037China 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)

年 卷 期：2023年第92卷第8期

页      面：2383-2397页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the Youth Foundation of the Natural Science Foundation of Jiangsu Province(BK20210614) the Nature Science Foundation of China(32071784) Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD) the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21_0921) 

主　　题：Liriodendron PIN2 auxin polar transport root development 

摘      要：The auxin polar transporter,PIN-FORMED 2(PIN2)plays an important role in root ***,it remains unclear whether PIN2 genes form two Liriodendron species,***(LcPIN2)and ***(LtPIN2),are both involved in root development and whether and to what extent these two genes diverge in ***,we cloned and overexpressed LcPIN2 and LtPIN2 in Arabidopsis thaliana wild-type(WT)and Atpin2 *** and sequence analysis showed a small degree of differentiation between these two Liriodendron PIN2 ***-specific gene expression analysis indicated that both Liriodendron PIN2 genes were highly expressed in roots,implying a potential role in root ***,heterologous overexpression of LcPIN2 and LtPIN2 in Arabidopsis both significantly increased the root length compared to wild-type and empty ***,the root length defect in Atpin2 was complemented both by LcPIN2 and ***,heterologous overexpression of LcPIN2 and LtPIN2 cannot rescue the defect in root gravitropism of Atpin2 *** together,ourfindings unravel PIN2 genes from the magnoliids plant Liriodendron were functionally conserved with AtPIN2 in the dicotyledonous plant Arabidopsis in regard to the regulation of root length,but not root *** study also provides a potential target for genetic improvement of the root system in these valuable forest trees Liriodendron.