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Development of a High-throughput Sequencing Platform for Detection of Viral Encephalitis Pathogens Based on Amplicon Sequencing

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Biomedical and Environmental Sciences 2024年第3期37卷 294-302页

作者： ZHANG Ya Li SU Wen Zhe WANG Rui Chen LI Yan ZHANG Jun Feng LIU Sheng Hui HU Dan He XU Chong Xiao YIN Jia Yu YIN Qi Kai HE Ying LI Fan FU Shi Hong NIE Kai LIANG Guo Dong TAO yong XU song Tao MA Chao Feng WANG Huan Yu Shaanxi University of Chinese Medicine Xianyang 712000ShaanxiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Guangzhou Center for Disease Control and Prevention Guangzhou 510000GuangdongChina Shandong Center for Disease Control and Prevention Jinan 250000ShandongChina Department of Ophthalmology Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Xi'an Center for Disease Control and Prevention Xi’an 710054ShaanxiChina

Objective Viral encephalitis is an infectious disease severely affecting human *** is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other *** laboratory diagnosis of viral encephalitis is a worldwide ***,high-throughput sequencing technology has provided new tools for diagnosing central nervous system ***,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon *** We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant *** detection ability of the primers was verified by software simulation and the detection of known positive *** sequencing was used to validate the samples,and consistency was compared with Sanger *** The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted *** sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger *** Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral *** is also a useful tool for the high-volume screening of clinical samples.

关键词： Viral encephalitis Amplicon sequencing High-throughput sequencing Multipathogen detection

青藏高原县域尺度PM_(2.5)浓度时空动态演进特征

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地理学报 2024年第3期79卷 654-671页

作者：刘庆芳谢佳亮张先甜徐勇宋金平北京师范大学地理科学学部北京100875 华南理工大学旅游管理系广州510006 湖南师范大学旅游学院长沙410081 中国科学院地理科学与资源研究所北京100101

青藏高原地区的大气污染防治不仅是打赢蓝天保卫战的有力举措,也是维护青藏高原生态安全屏障、实现高原经济高质量发展和可持续发展的必然要求。基于长时间序列和高空间分辨率的PM_(2.5)数据,采用空间自相关模型分析青藏高原县域尺度PM_... 详细信息

青藏高原地区的大气污染防治不仅是打赢蓝天保卫战的有力举措,也是维护青藏高原生态安全屏障、实现高原经济高质量发展和可持续发展的必然要求。基于长时间序列和高空间分辨率的PM_(2.5)数据,采用空间自相关模型分析青藏高原县域尺度PM_(2.5)浓度的静态空间关联特征,结合传统和空间Kernel密度估计探究PM_(2.5)浓度的分布动态演进和长期转移趋势,最后运用空间马尔可夫链刻画青藏高原县域尺度PM_(2.5)浓度的空间转移特征。结果表明:(1)青藏高原县域尺度PM_(2.5)浓度起步较高且空间锁定特征显著,新疆南部、青海省的柴达木盆地、西宁市和海东市内区县始终是PM_(2.5)高排放地区;(2)青藏高原县域尺度PM_(2.5)浓度呈现显著的空间关联特征,局部空间关联主要以H-H和L-L两种同质性空间关联类型为主;(3)青藏高原县域尺度PM_(2.5)浓度呈现整体下降的分布动态演进特征,局部差异仍显较大,考虑相邻地区的空间影响时,表现出空间收敛模式和显著的空间正相关性;3期时间滞后条件下,PM_(2.5)浓度高值区的虹吸效应增强,地区之间的相互作用影响着青藏高原PM_(2.5)浓度的未来空间分布。(4)青藏高原县域尺度PM_(2.5)浓度的空间转移趋势稳定,难以实现县域尺度单元上的跳跃式转移。本文可为识别青藏高原县域尺度PM_(2.5)浓度空间动态演进特征,实施精准化的大气污染防治政策,进而促进高原地区大气污染防控和生态文明建设提供理论参考。

关键词： PM_(2.5) 空间关联动态演进空间动态Kernel密度估计青藏高原

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慢性疼痛基因治疗相关离子通道的研究进展

中国疼痛医学杂志 2024年第8期30卷 598-603页

作者：樊玉昂栾永宋辛叶大连医科大学大连116044 大连医科大学附属第一医院麻醉科大连116011

慢性疼痛严重影响病人生活质量且造成巨大经济负担,现有的治疗方式不能很好的满足病人的镇痛需求。近年来,人们对于生物基因组有了更加全面的认识,同时精准递送系统的研发为基因治疗提供了有效手段,可以靶向递送抗伤害感受性分子或阻断... 详细信息

慢性疼痛严重影响病人生活质量且造成巨大经济负担,现有的治疗方式不能很好的满足病人的镇痛需求。近年来,人们对于生物基因组有了更加全面的认识,同时精准递送系统的研发为基因治疗提供了有效手段,可以靶向递送抗伤害感受性分子或阻断痛觉传导。目前,已发现多种疼痛相关靶点,不同靶点介导不同类型的疼痛,为基因治疗慢性疼痛开辟了新的思路。新兴的基因疗法应用前景广阔,但也面临巨大的挑战。本文主要总结了与慢性疼痛基因治疗相关的离子通道靶点,为进一步寻找特异性靶点提供思路。

关键词：慢性疼痛基因治疗离子通道靶点

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基于虾螯结构的仿生夹层板设计及数值模拟

吉林大学学报（工学版） 2024年第3期54卷 842-851页

作者：杨欣王阳宋家锋朱勇黄彬兵许述财河北农业大学机电工程学院河北保定071001 清华大学苏州汽车研究院(相城) 江苏苏州215134 清华大学智能绿色车辆与交通全国重点实验室北京100084 运输车辆检测、诊断与维修技术交通行业重点实验室济南250357 清华大学车辆与运载学院北京100084

探索了基于虾螯轮廓外形设计的仿生夹层板(BSP)的耐撞性。按照拉伸试验获得的材料本构参数设置了Hyperworks有限元模型,制造了BSP样件,并试验验证了有限元模型有效性。比较了BSP-L0、BSP-L180、BSP-T0、BSP-T180四种配置方案的耐撞性指... 详细信息

探索了基于虾螯轮廓外形设计的仿生夹层板(BSP)的耐撞性。按照拉伸试验获得的材料本构参数设置了Hyperworks有限元模型,制造了BSP样件,并试验验证了有限元模型有效性。比较了BSP-L0、BSP-L180、BSP-T0、BSP-T180四种配置方案的耐撞性指标,选择BSP-T0方案进一步研究。分析了圆弧高度比γ和梯形晶元窄底宽度B对BSP-T0耐撞性的影响规律,得到γ比B对BSP-T0耐撞性的影响更大。利用遗传学算法进行参数优化,Pareto前沿的一个最优解为比吸能SEA=3.080 kJ/kg、峰值载荷PF=30.700 kN、平均压溃效率CFE=86.884%,相应参数γ=0.355,B=4.23 mm,预测与仿真结果误差均在2%以内,预测效果理想,本文研究成果可为夹层结构设计提供新思路。

关键词：工程仿生学夹层结构耐撞性多目标优化遗传学算法

A multicenter prospective study on the management of hepatoblastoma in children:a report from the Chinese Children's Cancer Group

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World Journal of Pediatrics 2024年第6期20卷 590-601页

作者： Meng-Jie Tang Xiao-Li Ma Xiang-Ling He Wei-Hua Pan Xiao-Hong Zhang Sha-Yi Jiang Ju Gao Fu Lie Wei Yao song Gu Wei-Ling Zhang Qiang Zhao Shi-Hao Huang yong-Jun Fang Wei Liu Hui-Zhong Niu Chun-Mei Wang Li-Rong Sun Hui Gao Yun-Peng Dai Shun-Gen Huang Zhi-yong Zhong Xi-Ge Wang Zhong-Rong Li Liang-Chun Yang Ye-Ming Wu Huan-Min Wang Xin Sun Xiao-Jun Yuan Department of Pediatric Hematology/Oncology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineNo.1665Kongjiang RoadYangpu DistrictShanghai 200092China Medical Oncology Department Pediatric Oncology CenterBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China Department of Hematology/Oncology Children's Medical CenterHunan Provincial People's HospitalThe First Affiliated Hospital of Hunan Normal UniversityChangsha 41005China Department of Pediatric Surgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Hematology/Oncology Guangzhou Women and Children's Medical CenterNational Children's Medical Center for South Central RegionGuangzhou 510623China Department of Hematology Shanghai Children's HospitalShanghai Jiao Tong UniversityShanghai 200062China Department of Pediatrics West China Second University HospitalSichuan UniversityChengdu 610041China Department of Hematology/Oncology Qilu Children's Hospital of Shandong UniversityJinan 250022China Department of Pediatric Surgery Children's Hospital of Fudan UniversityShanghai 201102China Department of General Surgery Shanghai Children's Medical Center(National Children's Medical Center-Shanghai)Shanghai Jiao Tong University School of MedicineShanghai 200127China Department of Pediatrics Beijing Tongren HospitalCapital Medical UniversityBeijing 100730China Department of Pediatric Oncology Tianjin Medical University Cancer Institute and HospitalTianjin 300060China Department of Hematology/Oncology Children's Hospital of Nanjing Medical UniversityNanjing 210008China Department of Hematology/Oncology Henan Children's HospitalZhengzhou 450018China Department of Pediatric General Surgery Hebei Children's Hospital of Hebei Medical UniversityShijiazhuang City 050031China Children's Hospital the First Affliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of Paediatric

Background This study aimed to identify survival risk factors in Chinese children with hepatoblastoma(HB)and assess the effectiveness of the new treatment protocol proposed by the Chinese Children's Cancer Group(CCCG)in *** A multicenter,prospective study that included 399 patients with HB from January 2015 to June 2020 was *** demographics,treatment protocols,and other related information were *** regression models and Kaplan-Meier curve methods were *** The 4-year event-free survival(EFS)and overall survival(OS)were 76.9 and 93.5%,*** 4-year EFS rates for the very-low-risk,low-risk,intermediate-risk,and high-risk groups were 100%,91.6%,81.7%,and 51.0%,*** 4-year 0S was 100%,97.3%,94.4%,and 86.8%,*** regression analysis found that age,tumor rupture(R+),and extrahepatic tumor extension(E+)were independent prognostic factors.A total of 299 patients had complete remission,and 19 *** with declining alpha-fetoprotein(AFP)>75%after the first two cycles of neoadjuvant chemotherapy had a better EFS and OS than those≤75%.Conclusions The survival outcome of HB children has dramatically improved since the implementation of CCCG-HB-2016 ***≥8 years,R+,and E+were independent risk factors for *** with a declining AFP>75%after the first two cycles of neoadjuvant chemotherapy had better EFS and OS.

关键词： Alpha-fetoprotein Hepatoblastoma Multicenter Prospective study Survival

Stratigraphic framework and sedimentary evolution during the Cryogenian-Ediacaran transition in northeastern Sichuan Basin,South China

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Petroleum Science 2024年第2期21卷 823-835页

作者： Yi Zhang Hong-Wei Kuang yong-Qing Liu Qiang Shi Dong-Ge Wang Ke-Ning Qi Yu-Chong Wang Da-Wei Qiao Xiao-Shuai Chen Li-Zhi Wu Meng Tian Long Chen Yi Wei Liao-Yuan song Jian Li Zi-Gang Wu Yun-Qian Liu Xuan-Chun Liu An-Qing Chen Zhi-Wei Liao Institute of Geology Chinese Academy of Geological SciencesBeijing100037China State Key Laboratory of Coal Mine Disaster Dynamics and Control School of Resources and Safety EngineeringChongqing UniversityChongqing400044China No.205 Geological Team Chongqing Bureau of Geology and Minerals ExplorationChongqing401121China Chongqing Institute of Geological Survey Chongqing401122China Key Laboratory of Deep-time Geography and Environment Reconstruction and Applications of Ministry of Natural Resources Chengdu University of TechnologyChengdu610059SichuanChina

The northeastern Sichuan area in the northern Yangtze margin has unique Ediacaran geological records,especially the Doushantuo Formation(DST),and become a hot research area in recent ***,the Cryogenian-Ediacaran(C-E)boundary has not been precisely identified,which restricts the in-depth study of geological information during this crucial transitional period and is unfavorable for a systematic and complete understanding of the Yangtze Block and even the global paleogeographic *** study conducted stratigraphy,sedimentology,and chronostratigraphy to establish the stratigraphic framework and sedimentary evolution of the C-E transition strata in northeastern *** results showed that the Ediacaran sediments,without the cap dolomite,unconformably overlaid the Cryogenian sediments in the studied *** Member II of the DST,characterized by 50-160 m of red-green sandstone(approximately equivalent to the original Chengkou“Guanyinya Formation”),directly overlaid the Cryogenian sediments and displayed a 623±2.3 Ma maximum depositional age from the detrital zircon U-Pb *** stratigraphic correlations indicate that the C-E transition strata in northeastern Sichuan had a consistent lithological association and sedimentary sequence characteristics but differed from the Three ***,the upper Nantuo massive glacial diamictites transition to the icebergs rafted lonestone-bearing mudstones at the top,then change upward to DST barrier coast *** proposed DST of the northeastern Sichuan Basin was divided into three lithostratigraphic members without the regional Member I cap dolomite:(i)Member II purple-red,gray-green sandstone strata,(ii)MemberⅢblack mudstone strata,and(iii)Member IV P-Mn bearing *** the C-E transition,the study area experienced(i)the global deglaciation stage in the terminal Marinoan glaciation and(ii)the filling-leveling up stage with clastic rocks in the early ***,the early Ediacaran

关键词： Marinoan glaciation Doushantuo formation Cap dolomite Deglacial and coastal deposits Detrital zircon U-Pb dating

Wedge-shaped HfO_(2) buffer layer-induced field-free spin-orbit torque switching of HfO_(2)/Pt/Co structure

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Chinese Physics B 2024年第4期33卷 662-667页

作者：陈建辉梁梦凡宋衍袁俊杰张梦旸骆泳铭王宁宁 School of Electronics and Information Engineering Hangzhou Dianzi UniversityHangzhou 310018China Covestro lnvestment Co. LtdShanghai 200126China

Field-free spin-orbit torque(SOT)switching of perpendicular magnetization is essential for future spintronic *** study demonstrates the field-free switching of perpendicular magnetization in an HfO_(2)/Pt/Co/TaO_(x) structure,which is facilitated by a wedge-shaped HfO_(2)buffer *** field-free switching ratio varies with HfO_(2)thickness,reaching optimal performance at 25 *** phenomenon is attributed to the lateral anisotropy gradient of the Co layer,which is induced by the wedge-shaped HfO_(2)buffer *** thickness gradient of HfO_(2)along the wedge creates a corresponding lateral anisotropy gradient in the Co layer,correlating with the switching *** findings indicate that field-free SOT switching can be achieved through designing buffer layer,offering a novel approach to innovating spin-orbit device.

关键词： spin-orbit torque field-free switching HfO_(2) buffer layer

Critical behavior of quasi-two-dimensional ferromagnet Cr1.04Te2

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Chinese Physics B 2024年第7期33卷 97-105页

作者：钮伟宋沁心常世琦王敏袁奎高嘉程王硕王振东刘凯斐刘萍徐永兵张晓倩普勇 Jiangsu Provincial Engineering Research Center of Low Dimensional Physics and New Energy & School of Science Nanjing University of Posts and Telecommunications School of Electronic Science and Engineering Nanjing University Key Laboratory of Quantum Materials and Devices of Ministry of Education School of Physics Southeast University

The self-intercalation of Cr into pristine two-dimensional（2D） van der Waals ferromagnetic CrTe2,which forms chromium tellurides（CrxTe2）,has garnered interest due to their remarkable magnetic characteristics and the wide variety of chemical compositions ***,comprehensive basic characterization and magnetic studies are conducted on quasi-2D ferromagnetic *** of the isothermal magnetization curves are conducted around the critical temperature to systematically investigate the critical ***,the critical exponents β=0.2399,γ=0.859,and δ=4.3498,as well as the Curie temperature TC=249.56 K,are determined using various methods,including the modified Arrott plots,the Kouvel-Fisher method,the Widom scaling method,and the critical isotherm *** results indicate that the tricritical mean-field model accurately represents the critical behavior of Cr1.04Te2.A magnetic phase diagram with tricritical phenomenon is thus *** investigations confirm that the critical exponents obtained conform to the scalar equation near TC,indicating their self-consistency and *** work sheds light on the magnetic properties of quasi-2D Cr1.04Te2,broadening the scope of the van der Waals crystals for developments of future spintronic devices operable at room temperature.

关键词： critical behavior magnetic measurement self-intercalation van der Waals magnets

一步法合成P4HB基热塑性聚氨酯弹性体及其性质

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功能高分子学报 2024年第3期37卷 187-195页

作者：谭智宇宋红玮沈勇李志波青岛科技大学化工学院青岛科技大学高分子科学与工程学院山东省教育厅生物基高分子材料重点实验室

以聚(4-羟基丁酸酯)(P4HB)为软段、不同异氰酸酯和1,4-丁二醇(BDO)为硬段,通过一步法合成了一系列可解聚回收的P4HB基聚氨酯(P4HB-TPU)。研究了投料比、P4HB的数均分子量、扩链剂BDO含量以及异氰酸酯种类对聚氨酯性能的影响,以及P4HB-TP... 详细信息

以聚(4-羟基丁酸酯)(P4HB)为软段、不同异氰酸酯和1,4-丁二醇(BDO)为硬段,通过一步法合成了一系列可解聚回收的P4HB基聚氨酯(P4HB-TPU)。研究了投料比、P4HB的数均分子量、扩链剂BDO含量以及异氰酸酯种类对聚氨酯性能的影响,以及P4HB-TPU的可控解聚与单体回收。采用傅里叶变换红外光谱、热重分析、差示扫描量热、单轴机械拉伸等对P4HBTPU的热性能、力学性能以及热解聚回收性进行了研究。结果表明,当投料配方设计中n(—NCO)/n(—OH)为1.1、异氰酸酯为二环己基甲烷-4,4’-二异氰酸酯(HMDI)、P4HB的数均分子量为1.9×103时,P4HB-TPU的拉伸强度可达到37.8 MPa,性能与商用聚己内酯基热塑性聚氨酯(PCL-TPU)相近。随着P4HB分子量的增大,其结晶性使得聚氨酯由弹性体开始向塑料转变。BDO含量的增加在一定程度上有助于提高TPU的力学性能。使用环状对称的异氰酸酯可以有效提升材料的拉伸强度。在170℃、200 Pa的条件下,通过对P4HB-TPU热解聚,可以将P4HB解聚为γ-丁内酯(γ-BL)单体,并达到约98%的单体回收率。

关键词：聚酯聚氨酯聚(4-羟基丁酸酯) 闭合循环聚氨酯解聚热塑性聚氨酯

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