Development of a High-throughput Sequencing Platform for Detection of Viral Encephalitis Pathogens Based on Amplicon Sequencing

作     者：ZHANG Ya Li SU Wen Zhe WANG Rui Chen LI Yan ZHANG Jun Feng LIU Sheng Hui HU Dan He XU Chong Xiao YIN Jia Yu YIN Qi Kai HE Ying LI Fan FU Shi Hong NIE Kai LIANG Guo Dong TAO Yong XU Song Tao MA Chao Feng WANG Huan Yu ZHANG Ya Li;SU Wen Zhe;WANG Rui Chen;LI Yan;ZHANG Jun Feng;LIU Sheng Hui;HU Dan He;XU Chong Xiao;YIN Jia Yu;YIN Qi Kai;HE Ying;LI Fan;FU Shi Hong;NIE Kai;LIANG Guo Dong;TAO Yong;XU Song Tao;MA Chao Feng;WANG Huan Yu

作者机构：Shaanxi University of Chinese MedicineXianyang 712000ShaanxiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Guangzhou Center for Disease Control and PreventionGuangzhou 510000GuangdongChina Shandong Center for Disease Control and PreventionJinan 250000ShandongChina Department of OphthalmologyBeijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Xi'an Center for Disease Control and PreventionXi’an 710054ShaanxiChina 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2024年第37卷第3期

页      面：294-302页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0711[理学-系统科学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 07[理学] 08[工学] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：supported by the National Key Research and Development Program(grant number:2022YFC2305304) 

主　　题：Viral encephalitis Amplicon sequencing High-throughput sequencing Multipathogen detection 

摘      要：Objective Viral encephalitis is an infectious disease severely affecting human *** is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other *** laboratory diagnosis of viral encephalitis is a worldwide ***,high-throughput sequencing technology has provided new tools for diagnosing central nervous system ***,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon *** We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant *** detection ability of the primers was verified by software simulation and the detection of known positive *** sequencing was used to validate the samples,and consistency was compared with Sanger *** The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted *** sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger *** Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral *** is also a useful tool for the high-volume screening of clinical samples.