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QTL analysis of soft scald in two apple populations

Horticulture Research 2016年第1期3卷 117-123页

作者： Kendra A McClure Kyle M Gardner Peter MA Toivonen Cheryl R Hampson Jun Song Charles F Forney John DeLong Istvan Rajcan sean myles Department of Plant and Animal Sciences Faculty of AgricultureDalhousie UniversityTruroB2N 5E3Nova ScotiaCanada Department of Plant Agriculture Crop Science BuildingUniversity of GuelphGuelphN1G 2W1OntarioCanada Agriculture and Agri-Food Canada Fredericton Research and Development CentreFrederictonE3B 4Z7New BrunswickCanada Agriculture and Agri-Food Canada Summerland Research and Development CentreSummerlandV0H 1Z0British ColumbiaCanada Agriculture and Agri-Food Canada Kentville Research and Development CentreKentvilleB4N 1J5Nova ScotiaCanada

The apple(Malus×domestica Borkh.)is one of the world’s most widely grown and valuable fruit *** demand for apples year round,storability has emerged as an important consideration for apple breeding *** scald is a cold storage-related disorder that results in sunken,darkened tissue on the fruit *** breeders are keen to generate new cultivars that do not suffer from soft scald and can thus be marketed year *** breeding approaches are protracted and labor intensive,and therefore marker-assisted selection(MAS)is a valuable tool for *** advance MAS for storage disorders in apple,we used genotyping-by-sequencing(GBS)to generate high-density genetic maps in two F1 apple populations,which were then used for quantitative trait locus(QTL)mapping of soft *** total,900 million DNA sequence reads were generated,but after several data filtering steps,only 2%of reads were ultimately used to create two genetic maps that included 1918 and 2818 single-nucleotide *** QTL associated with soft scald were identified in one of the bi-parental populations originating from parent 11W-12-11,an advanced breeding *** study demonstrates the utility of next-generation DNA sequencing technologies for QTL mapping in F1 populations,and provides a basis for the advancement of MAS to improve storability of apples.

关键词： breeding cultivar apple

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Genomic consequences of apple improvement

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Horticulture Research 2021年第1期8卷 97-109页

作者： Zoe Migicovsky Kyle M.Gardner Christopher Richards C.Thomas Chao Heidi R.Schwaninger Gennaro Fazio Gan-Yuan Zhong sean myles Department of Plant Foodand Environmental SciencesFaculty of AgricultureDalhousie UniversityTruroNSCanada USDA-ARS Plant Germplasm Preservation Research UnitFort CollinsCOUSA USDA-ARS Grape Genetics Research UnitGen evaNYUSA Present address:Agriculture and Agri-Food Canada Fredericton Research and Development CentreFrederictonNBCanada

The apple(Malus domestica)is one of the world's most commercially important perennial crops and its improvement has been the focus of human effort for thousands of ***,we genetically characterise over 1000 apple accessions from the United States Department of Agriculture(USDA)germplasm collection using over 30,000 singlenucleotide polymorphisms(SNPs).We con fi rm the close genetic relationship between modern apple cultivars and their primary progenitor species,Malus sieversii from Central Asia,and fi nd that cider apples derive more of their ancestry from the European crabapple,Malus sylvestris,than do dessert *** determine that most of the USDA collection is a large complex pedigree:over half of the collection is interconnected by a series of fi rst-degree *** addition,15%of the accessions have a fi rst-degree relationship with one of the top 8 cultivars produced in the *** the exception of‘Honeycrisp',the top 8 cultivars are interconnected to each other via pedigree *** cultivars‘Golden Delicious'and‘Red Delicious'were found to have over 60 fi rst-degree relatives,consistent with their repeated use by apple *** detected a signature of intense selection for red skin and provide evidence that breeders also selected for increased fi *** results suggest that Americans are eating apples largely from a single family tree and that the apple's future improvement will bene fi t from increased exploitation of its tremendous natural genetic diversity.

关键词： cultivar crops consequences

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Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols

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Horticulture Research 2019年第1期6卷 444-455页

作者： Kendra AMcClure YuiHui Gong Jun Song Melinda Vinqvist-Tymchuk Leslie Campbell Palmer Lihua Fan Karen Burgher-MacLellan ZhaoQi Zhang Jean-Marc Celton Charles FForney ZoëMigicovsky sean myles Department of Plant and Animal Sciences Faculty of AgricultureDalhousie UniversityTruroNS B2N 5E3Canada Agriculture and Agri-Food Canada Kentville Research and Development CentreKentvilleNS B4N 1J5Canada College of Horticulture South China Agriculture UniversityGuangzhou 510642China IRHS Agrocampus-OuestINRAUniversitéd’AngersSFR 4207 QuaSaVBeaucouzéFrance

Apples are a nutritious food source with significant amounts of polyphenols that contribute to human health and wellbeing,primarily as dietary *** numerous pre-and post-harvest factors can affect the composition of polyphenols in apples,genetics is presumed to play a major role because polyphenol concentration varies dramatically among apple *** we investigated the genetic architecture of apple polyphenols by combining high performance liquid chromatography(HPLC)data with~100,000 single nucleotide polymorphisms(SNPs)from two diverse apple *** found that polyphenols can vary in concentration by up to two orders of magnitude across cultivars,and that this dramatic variation was often predictable using genetic markers and frequently controlled by a small number of large effect genetic *** GWAS,we identified candidate genes for the production of quercitrin,epicatechin,catechin,chlorogenic acid,4-O-caffeoylquinic acid and procyanidins B1,B2,and *** observation that a relatively simple genetic architecture underlies the dramatic variation of key polyphenols in apples suggests that breeders may be able to improve the nutritional value of apples through markerassisted breeding or gene editing.

关键词： effect apple cultivar

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Patterns of genomic and phenomic diversity in wine and table grapes

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Horticulture Research 2017年第1期4卷 181-191页

作者： ZoëMigicovsky Jason Sawler Kyle M Gardner Mallikarjuna K Aradhya Bernard H Prins Heidi R Schwaninger Carlos D Bustamante Edward S Buckler Gan-Yuan Zhong Patrick J Brown sean myles Department of Plant Food and Environmental SciencesFaculty of AgricultureDalhousie UniversityTruroB2N 5E3NSCanada Anandia Labs VancouverV6T 1Z4BCCanada Agriculture and Agri-Food Canada Fredericton Research and Development CentreFrederictonE3B 4Z7NBCanada United States Department of Agriculture-Agricultural Research Service National Clonal Germplasm RepositoryUniversity of CaliforniaDavis95616CAUSA United States Department of Agriculture Agricultural Research ServiceGrape Genetics Research UnitNew York State Agricultural Experiment StationCornell UniversityGeneva14456NYUSA Department of Genetics Stanford UniversityStanfordCA 94305USA Department of Plant Breeding and Genetics Cornell UniversityIthaca14853NYUSA United States Department of Agriculture Agricultural Research ServicePlant Genetic Resources UnitNew York State Agricultural Experiment StationCornell UniversityGeneva14456NYUSA Department of Crop Science University of IllinoisUrbana61801ILUSA

Grapes are one of the most economically and culturally important crops worldwide,and they have been bred for both winemaking and fresh *** we evaluate patterns of diversity across 33 phenotypes collected over a 17-year period from 580 table and wine grape accessions that belong to one of the world’s largest grape gene banks,the grape germplasm collection of the United States Department of *** find that phenological events throughout the growing season are correlated,and quantify the marked difference in size between table and wine *** pairing publicly available historical phenotype data with genome-wide polymorphism data,we identify large effect loci controlling traits that have been targeted during domestication and breeding,including hermaphroditism,lighter skin pigmentation and muscat *** for larger berries in table grapes was traditionally concentrated in geographic regions where Islam predominates and alcohol was prohibited,whereas wine grapes retained the ancestral smaller size that is more desirable for winemaking in predominantly Christian *** uncover a novel locus with a suggestive association with berry size that harbors a signature of positive selection for larger *** results suggest that religious rules concerning alcohol consumption have had a marked impact on patterns of phenomic and genomic diversity in grapes.

关键词： harbor religious crops

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Apple whole genome sequences:recent advances and new prospects

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Horticulture Research 2019年第1期6卷 1247-1270页

作者： Cameron P.Peace Luca Bianco Michela Troggio Eric van de Weg Nicholas P.Howard Amandine Cornille Charles-Eric Durel sean myles ZoëMigicovsky Robert J.Schaffer Evelyne Costes Gennaro Fazio Hisayo Yamane Steve van Nocker Chris Gottschalk Fabrizio Costa David Chagné Xinzhong Zhang Andrea Patocchi Susan E.Gardiner Craig Hardner Satish Kumar Francois Laurens Etienne Bucher Dorrie Main Sook Jung Stijn Vanderzande Department of Horticulture Washington State UniversityPullmanWA 99164USA Computational Biology Fondazione Edmund MachSan Michele all’AdigeTN 38010Italy Department of Genomics and Biology of Fruit Crops Fondazione Edmund MachSan Michele all’AdigeTN 38010Italy Plant Breeding Wageningen University and ResearchWageningen 6708PBThe Netherlands Department of Horticultural Science University of MinnesotaSt.PaulMN 55108USA Institut für Biologie und Umweltwissenschaften Carl von Ossietzky Universität26129 OldenburgGermany GQE-Le Moulon Institut National de la Recherche AgronomiqueUniversity of Paris-SudCNRSAgroParisTechUniversitéParis-Saclay91190 Gif-sur-YvetteFrance Institut National de la Recherche Agronomique Institut de Recherche en Horticulture et SemencesUMR 134549071 BeaucouzéFrance Department of Plant Food and Environmental SciencesFaculty of AgricultureDalhousie UniversityTruroNS B2N 5E3Canada The New Zealand Institute for Plant and Food Research Ltd Motueka 7198New Zealand School of Biological Sciences University of AucklandAuckland 1142New Zealand AGAP INRACIRADMontpellier SupAgroUniversity of MontpellierMontpellierFrance Plant Genetic Resources Unit USDA ARSGenevaNY 14456USA Laboratory of Pomology Graduate School of AgricultureKyoto UniversityKyoto 606-8502Japan Department of Horticulture Michigan State UniversityEast LansingMI 48824USA The New Zealand Institute for Plant and Food Research Ltd(Plant&Food Research) Palmerston North Research CentrePalmerston North 4474New Zealand College of Horticulture China Agricultural University100193 BeijingChina Agroscope 8820 WädenswilSwitzerland Queensland Alliance of Agriculture and Food Innovation University of QueenslandSt Lucia 4072Australia New Cultivar Innovation Plant and Food ResearchHavelock North 4130New Zealand Agroscope 1260 ChanginsSwitzerland

In 2010,a major scientific milestone was achieved for tree fruit crops:publication of the first draft whole genome sequence(WGS)for apple(Malus domestica).This WGS,v1.0,was valuable as the initial reference for sequence information,fine mapping,gene discovery,variant discovery,and tool development.A new,high quality apple WGS,GDDH13 v1.1,was released in 2017 and now serves as the reference genome for *** the past decade,these apple WGSs have had an enormous impact on our understanding of apple biological functioning,trait physiology and inheritance,leading to practical applications for improving this highly valued *** gene identities for phenotypes of fundamental and practical interest can today be discovered much more ***-wide polymorphisms at high genetic resolution are screened efficiently over hundreds to thousands of individuals with new insights into genetic relationships and ***-density genetic maps are constructed efficiently and quantitative trait loci for valuable traits are readily associated with positional candidate genes and/or converted into diagnostic tests for *** understand the species,geographical,and genomic origins of domesticated apple more precisely,as well as its relationship to wild *** WGS has turbo-charged application of these classical research steps to crop improvement and drives innovative methods to achieve more durable,environmentally sound,productive,and consumer-desirable apple *** review includes examples of basic and practical breakthroughs and challenges in using the apple *** for“what’s next”focus on necessary upgrades to the genome sequence data pool,as well as for use of the data,to reach new frontiers in genomics-based scientific understanding of apple.

关键词： breakthrough hundreds frontier

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Blood biomarkers of Alzheimer’s disease:important considerations for use in clinical practice

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Neural Regeneration Research 2025年第1期20卷 205-206页

作者： Sarah Fullam sean O’Dowd Antoinette O’Connor Department of Neurology Tallaght University HospitalDublinIreland Academic Unit of Neurology Trinity College DublinIreland

Introduction:Fluid and positron emission tomography(PET)biomarkers that enable the detection of the hallmark proteins of Alzheimer’s disease(AD)(amyloid and tau)have revolutionized our approach to the diagnosis of *** evolution of AD diagnostic criteria to include biological characterization(Alzheimer’s Association Working Group,2023)provides an appropriate framework to reduce levels of clinico-pathologic mismatch and improve in-vivo diagnostic *** the therapeutic landscape for neurodegenerative disease evolves,it is increasingly incumbent on clinicians to provide timely,and pathologically precise diagnoses for ***,the expensive and invasive nature of these tests limits their scalability.

关键词： Blood Alzheimer diagnosis

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Direct Air Carbon Capture Takes Baby Steps-Giant Strides Are Needed sean O’Neill

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Engineering 2022年第1期8卷 3-5页

作者： sean O’Neill 机构不详

In September 2021,the world’s largest industrial plant to pull carbon dioxide(CO_(2))directly out of the atmosphere officially became operational in *** and built by the Zurich,Switzerland-based company Climeworks,it... 详细信息

关键词： remove store permanent

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VOC removal from contaminated groundwater through membrane pervaporation. (Ⅱ): 1,1,1-trichloroethane-SDS surfactant solution system

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Journal of Environmental Sciences 2003年第6期15卷 821-826页

作者： sean LIU Department of Food Science Rutgers University

The conventional “pump-and-treat' technology for subsurface remediation of groundwater contaminated with volatile organic compounds(VOCs) such as 1,1,1-trichloroethane(TCA), a common chlorinated organic solvent, has limitation of prohibitively long treatment time due to extremely low water solubility of the VOCs. Surfactant-based soil remediation has emerged as the effective technology that substantially reduces the treatment time. In order to make the whole process economical, the surfactant used in soil washing has to be recovered and reused. This study examined the recovery of anionic surfactant, sodium dodecyl sulfate(SDS), from soil remediation fluids containing TCA, using a bench-scale membrane pervaporation unit. The effects of high TCA concentration, surfactant dosage, and flow rate on permeation flux and selectivity(α value) of the process were evaluated. In general, higher surfactant concentration yielded lower TCA flux and constant water flux, resulting in declining α values; higher flow rate of TCA feed stream results in higher VOC flux and selectivity, an indication of the effect of concentration polarization; higher TCA feed concentration produces higher TCA permeation across the membrane, however, the selectivity was virtually unchanged unless the total TCA concentration exceeded 2000 ppm.

关键词： pervaporation VOCs SDS surfactant groundwater remediation 1,1,1-trichloroethane

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XML by example building E-Commerce applications sean McGrath.

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1998年

作者： sean McGrath.

来源：畅想之星图书光盘版（南... 评论

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Assessment of semi-empirical mass transfer correlations for pervaporation treatment of wastewater contaminated with chlorinated hydrocarbons

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2006年第11期7卷 1911-1916页

作者： LIU sean X. Department of Food Science Rutgers University New Brunswick NJ 08901-8520 USA

Assessment of mass transfer characteristics of pervaporation （PV） treatment of wastewater contaminated with chlorinated hydrocarbons is of great importance for water treatment plant operators conducting initial evaluation, process optimization, and process economics. While a membrane plays a central role in pervaporation processes and separation efficiency, the mass transfer in the liquid layer next to the membrane surface is of equal, if not greater importance. It is one of the few process parameters that can be adjusted in situ to manipulate the outcome of a pervaporation process. In this study, a bench scale pervaporation experiment of removing a common chlorinated hydrocarbon from water was carried out and the results of it were compared to the ones based on well-known semi-empirical correlations. The mass transfer coefficients from the experiments, ranging from 0.8× 10^-5-2.5× 10^-5 m/s under the operating conditions, are higher than those predicted by the correlation. The corresponding separation factors under varying flow velocities are determined to be between 310-950.

关键词： Chlorinated hydrocarbon Mass transfer Pervaporation Wastewater treatment

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