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Induction of clusterin Expression by Neuronal Cell Death in Zebrafish

Journal of Genetics and Genomics 2014年第11期41卷 583-589页

作者： Yun-Mi Jeong Tae-Eun jin Jung-Hwa Choi Mi-Sun Lee Hyun-Taek Kim Kyu-Seok Hwang Doo-sang Park Hyun-Woo Oh Joong-Kook Choi Vladimir Korzh Melitta Schachner Kwan-Hee You cheol-Hee Kim Department of Biology Chungnam National University Korea Research Institute of Bioscience and Biotechnology Department of Biochemistry College of MedicineChungbuk National University Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research Keck Center for Collaborative Neuroscience and Department of Cell Biology and Neuroscience Rutgers University Center for Neuroscience Shantou University Medical College

Clusterin, a protein associated with multiple functions, is expressed in a wide variety of mammalian tissues. Although clusterin is known to be involved in neurodegenerative diseases, ageing, and tumorigenesis, a detailed analysis of the consequences of gain- or loss-of- function approaches has yet to be performed to understand the underlying mechanisms of clusterin functions. Since clusterin levels change in neurological diseases, it is likely that clusterin contributes to cell death and degeneration in general. Zebrafish was investigated as a model system to study human diseases. During development, zebrafish clusterin was expressed in the notochord and nervous system. Embryonic overexpression of clusterin by mRNA microinjection did not affect axis formation, whereas its knock-down by anti-sense morpholino treatment resulted in neuronal cell death. To analyze the function of clusterin in neurodegeneration, a transgenic zebrafish was investigated, in which nitroreductase expression is regulated under the control of a neuron-specifc huC promoter which is active between the stages of early neuronal precursors and mature neurons. Nitroreductase turns metronidazole into a cytotoxic agent that induces cell death within 12 h. After metronidazole treatment, transgenic zebrafish showed neuron-specific cell death. Interestingly, we also observed a dramatic induction of clusterin expression in the brain and spinal cord in these fish, suggesting a direct or indirect role of clusterin in neuronal cell death and thus, more generally, in neurodegeneration.

关键词： Clusterin Neuronal cell death Neurodegeneration Zebrafish

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Wetting Characteristics of Insect Wing Surfaces

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Journal of Bionic Engineering 2009年第1期6卷 63-70页

作者： Doyoung Byun Jongin Hong Saputra jin Hwan Ko Young Jong Lee Hoon cheol Park Bong-Kyu Byun Jennifer R.Lukes Department of Aerospace Information Engineering Artificial Muscle Research CenterKonkuk UniversitySeoul 143-701Republic of Korea Department of Mechanical Engineering and Applied Mechanics University of PennsylvaniaPA 19104USA Department of Advanced Technology Fusion Artificial Muscle Research CenterKonkuk UniversitySeoul 143-701Republic of Korea Korea National Arboretum PocheonKyoungki 487-821Republic of Korea

Biological tiny structures have been observed on many kinds of surfaces such as lotus leaves,which have an effect on the coloration of Morpho butterflies and enhance the hydrophobicity of natural *** investigated the micro-scale and nano-scale structures on the wing surfaces of insects and found that the hierarchical multiple roughness structures help in enhancing the *** examining 10 orders and 24 species of flying Pterygotan insects,we found that micro-scale and nano-scale structures typically exist on both the upper and lower wing surfaces of flying *** tiny structures such as denticle or setae on the insect wings enhance the hydrophobicity,thereby enabling the wings to be cleaned more *** the hydrophobic insect wings undergo a transition from Cassie to Wenzel states at pitch/size ratio of about *** order to examine the wetting characteristics on a rough surface,a biomimetic surface with micro-scale pillars is fabricated on a silicon wafer, which exhibits the same behavior as the insect wing,with the Cassie-Wenzel transition occurring consistently around a pitch/width value of 20.

关键词： insect wing superhydrophobicity mimicry hierarchical structure micro- and nano-scale structures Cassie-Wenzel transition

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Serum 25-hydroxyvitamin deficiency in middle-aged a cross-sectional study D levels and testosterone Korean men：

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Asian Journal of Andrology 2015年第2期17卷 324-328,I0012页

作者： Young jin Tak Jeong Gyu Lee Yun jin Kim Nam cheol Park sang Soo Kim sangyeoup Lee Byung Mann Cho Eun Hee Kong Dong Wook Jung Yu Hyeon Yi Department of Family Medicine Pusan National University School of Medicine Busan Korea Biomedical Research Institute Pusan National University Hospital Busan Korea Department of Urology Pusan National University School of Medicine Busan Korea Department of Internal Medicine Pusan National University School of Medicine Busan Korea Medical Education Unit Pusan National University School of Medicine Yangsan Korea Department of Preventive Medicine and Occupational Medicine Pusan National University School of Medicine Yangsan Korea Department of Family Medicine College of Medicine Kosin University Busan Korea.

Previous studies have demonstrated that male hypogonadism is associated with a low level of vitamin D. However, no reports have investigated the effects of vitamin D on testosterone levels in Korean men. Our aim was to investigate whether testosterone levels are associated with serum vitamin D levels and whether seasonal variation exists. This cross-sectional study analyzed serum 25-hydroxyvitamin D [25（OH）D], total testosterone （TT）, and free testosterone （FT） in 652 Korean men over 40 years of age who had undergone a comprehensive medical examination. The average age of the subjects was 56.7± 7.9 years, and the mean serum 25（OH）D, TT and FT levels were 21.23 ± 7.9 ng m1-1, 4.70 ±1.6 ng m1-1, and 8.12± 3.3 pg m1-1, respectively. In the multiple linear regression model, 25（OH）D showed positive association with TT （β =0.137, P〈 0.001） and FT （p =0.103, P= 0.008）. 25（OH） D and FT showed similar seasonal or monthly variation after adjustment for age. A vitamin D deficiency [25（OH）D 〈 20 ng m1-1] was associated with an increased risk of deficiencies of TT （〈2.30 ng ml-1）（odds ratio [OR]： 2.65; 95% confidence interval [CI]. 1.21-5.78, P = 0.014） and FT （〈6.50 pg ml-1）（OR： 1.44; 95% Ch 1.01-2.06 P = 0.048） after adjusting for age, season, body mass index, body composition, chronic disease, smoking, and alcohol use. In conclusion, we demonstrated a positive correlation between 25（OH）D and testosterone, which showed similar seasonal variation in Korean men.

关键词： 25-hydroxyvitamin D hypogonadism seasons testosterone vitamin D

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Anaphylactic shock induced by polyethylene glycol after bowel preparation for the colorectal cancer surgery:A case report

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World Journal of Clinical Cases 2023年第23期11卷 5589-5594页

作者： Go Woon Park Nahyeon Park Jung cheol Kuk Eung jin Shin Dae Ro Lim Department of General Surgery Soonchunhyang Bucheon HospitalBucheon 14584South Korea

BACKGROUND Polyethylene glycol(PEG)is widely used as an additive because of its hydrophilic and chemically inert ***,there are been increasing reports of PEG allergies,including anaphylaxis,although they are still *** case report aims to raise awareness,that the commonly used bowel cleansing agent containing PEG can cause serious allergic *** SUMMARY Prior to surgery for sigmoid colon cancer,a 63-year-old man was prescribed a bowel cleansing agent containing *** 30 min of ingestion,he developed symptoms of anaphylactic shock and did not respond to initial intramuscular epinephrine *** diagnosis of anaphylaxis to PEG,he was stabilized with fluid hydration and continuous norepinephrine *** While allergic reactions to PEG are rare,they can be ***,it is crucial for clinicians to be aware of this possibility and to diagnose and resuscitate patients immediately.

关键词： Polyethylene glycol Anaphylaxis Sigmoid colon cancer Bowel preparation Case report

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Inhibitory effect of sulfated polysaccharide from Sargassum thunbergii in PA-induced lipid accumulation in vitro

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Food Bioscience 2023年第1期51卷 308-315页

作者： Hyo-Geun Lee Min-cheol Kang You-jin Jeon Young-sang Kim Department of Marine Life Sciences Jeju National UniversityJeju63243Republic of Korea Marine Science Institute Jeju National UniversityJeju Self-Governing Province63333Republic of Korea Research Group of Food Processing Korea Food Research Institute245Nongsaengmyeong-roIseo-myeonWanju55365Republic of Korea

This study aimed to investigate the In vitro lipid inhibitory effects of sulfated polysaccharide fractions derived from fractional ethanol precipitate 1 of Celluclast assisted hydrolysate from Sargassum thunbergii(STCF1-PSs).Three polysaccharide fractions were obtained,and physical characteristics were determined by SEM,FTIR,and MALS *** analysis indicated that STCF1-PS3 contained high fucose and galactose *** intracellular lipid inhibitory effects of STCF1-PS3 were evaluated on PA-treated 3T3-L1 and HepG2 cells and was found to be ***,it suppressed intracellular triglyceride and free fatty acid levels in these *** blot analysis showed that the lipid inhibitory activity was achieved through the regulation of the adipogenesis and lipolysis pathway in PA-treated 3T3-L1 cells and the free fatty acid oxidation pathway in PAtreated HepG2 ***,sulfated polysaccharide from Sargassum thunbergii has a beneficial effect on intracellular lipid metabolism and it could be used as a functional ingredient in functional food industry.

关键词： Sargassum thunbergii Sulfated polysaccharides Adipogenesis Lipolysis Fatty acid oxidation

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Orthotic approach to prevention and management of diabetic foot:A narrative review

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World Journal of Diabetes 2022年第11期13卷 912-920页

作者： Min cheol Chang Yoo jin Choo In Sik Park Myung Woo Park Du Hwan Kim Rehabilitation Medicine Yeungnam UniversityDaegu 42415South Korea Korean Podiatry and Pedorthics Institute Goyang 10442South Korea Physical Medicine and Rehabilitation Chung-Ang UniversitySeoul 06973South Korea

Diabetic foot is a common complication affecting more than one-fifth of patients with *** not treated in time,it may lead to diabetic foot ulcers or Charcot *** the management of diabetic foot,shoe modifications and orthoses can be used to reduce pressure on the affected foot or provide the foot with increased *** addition,the shoe modifications and orthotic devices can relieve patient discomfort during *** shoe modifications include changing the insole material,modifying the heel height,adding a steel shank or rocker sole,and using in-depth ***,a walking brace or ankle-foot orthosis can be used to reduce the pressure on the affected *** purpose of this narrative review was to provide a reference guide to support clinicians in prescribing shoe modifications and foot orthoses to treat diabetic foot ulcers and Charcot arthropathy.

关键词： Diabetic foot Foot ulcers Charcot arthropathy Shoes Foot orthosis

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Effect of Isothermal Annealing on Nano Ti-Carbides Precipitation

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Journal of Iron and Steel Research International 2011年第S1期18卷 287-289页

作者： cheol-ho Park Woo-jin Kim Nam-hyun Kang Hyung-hyup Do Kyung-Mox Cho Department of Materials Science and Engineering Pusan National UniversityBusan 609-735Korea Technical Research Laboratories Hyundai steelsDangjinChungnam 343-711Korea

Transmission electron microscopy was used to investigate the effect of isothermal holding temperature and time on the nano Ti-precipitates.A holding temperature was varied systematically from 400℃ to 1200℃.The isothermal holding was continued for 30s,300s and 900s,*** carbides of (Ti,Nb)C were precipitated significantly at 900℃.The size of carbides was approximately 10nm at 30s holding and increased to 20～30nm at 900s *** holding at 1000℃ showed the increased amount of carbides larger than *** 800℃,nano (Ti,Nb)C was not observed at 30s and it was examined at *** size of nano (Ti,Nb)C was smaller than that of 900℃.As the isothermal temperature decreased to 700 ℃,the nano (Ti,Nb)C was only seen at 900s holding and the size of carbides was smaller than *** (Ti,Nb)C was disappeared at isothermal holding below 600℃.The kinetics of nano (Ti,Nb)C precipitation were studied as a function of isothermal holding temperature and time,respectively,using the precipitate growth equations.

关键词： Ti carbide hot rolled steel hole expansion ratio isothermal holding nano precipitates

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RGD peptide and graphene oxide co-functionalized PLGA nanofiber scaffolds for vascular tissue engineering

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Regenerative Biomaterials 2017年第3期4卷 159-166页

作者： Yong cheol Shin Jeonghyo Kim Sung Eun Kim Su-jin Song SuckWon Hong jin-Woo Oh Jaebeom Lee Jong-Chul Park Suong-Hyu Hyon Dong-Wook Han Department of Cogno-Mechatronics Engineering Department of Optics and Mechatronics Engineering Department of Nanoenergy Engineering College of Nanoscience and NanotechnologyPusan National UniversityBusan 46241Korea Cellbiocontrol Laboratory Department of Medical EngineeringYonsei University College of MedicineSeoul 03722Korea and 5Center for Fiber and Textile Science Kyoto Institute of TechnologyMatsugasakiKyoto 606-8585Japan

In recent years,much research has been suggested and examined for the development of tissue engineering scaffolds to promote cellular *** our study,RGD peptide and graphene oxide(GO)co-functionalized poly(lactide-co-glycolide,PLGA)(RGD-GO-PLGA)nanofiber mats were fabricated via electrospinning,and their physicochemical and thermal properties were characterized to explore their potential as biofunctional scaffolds for vascular tissue *** electron microscopy images revealed that the RGD-GO-PLGA nanofiber mats were readily fabricated and composed of randomoriented electrospun nanofibers with average diameter of *** successful co-functionalization of RGD peptide and GO into the PLGA nanofibers was confirmed by Fourier-transform infrared spectroscopic ***,the surface hydrophilicity of the nanofiber mats was markedly increased by co-functionalizing with RGD peptide and *** was found that the mats were thermally stable under the cell culture ***,the initial attachment and proliferation of primarily cultured vascular smoothmuscle cells(VSMCs)on the RGD-GO-PLGA nanofibermats were *** was revealed that the RGD-GO-PLGA nanofibermats can effectively promote the growth of *** conclusion,our findings suggest that the RGD-GO-PLGA nanofiber mats can be promising candidates for tissue engineering scaffolds effective for the regeneration of vascular smooth muscle.

关键词： RGD peptide graphene oxide poly(lactic-co-glycolic acid) biofunctional scaffold vascular smooth muscle cell

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A Web-based machining process monitoring system for E-manufacturing implementation

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2006年第9期7卷 1467-1473页

作者： SHIN Bong-cheol KIM Gun-hee CHOI jin-hwa JEON Byung-cheol LEE Honghee CHO Myeong-woo HAN jin-yong PARK Dong-sam Division of Mechanical Engineering Inha University Incheon 402-751 Korea Department of Mechanical Engineering University of Incheon Incheon 402-749 Korea

Recently, with the rapid growth of information technology, many studies have been performed to implement Web-based manufacturing system. Such technologies are expected to meet the need of many manufacturing industries who want to adopt E-manufacturing system for the construction of globalization, agility, and digitalization to cope with the rapid changing market requirements. In this research, a real-time Web-based machine tool and machining process monitoring system is developed as the first step for implementing E-manufacturing system. In this system, the current variations of the main spindle and feeding motors are measured using hall sensors. And the relationship between the cutting force and the spindle motor RMS (Root Mean Square) current at various spindle rotational speeds is obtained. Thermocouples are used to measure temperature variations of important heat sources of a machine tool. Also, a rule-based expert system is applied in order to decide the machining process and machine tool are in normal conditions. Finally, the effectiveness of the developed system is verified through a series of experiments.

关键词： E-manufacturing Web-based system Internet Machining process monitoring Expert system

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Two- and Three-Dimensional Simulations of Beetle Hind Wing Flapping during Free Forward Flight

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Journal of Bionic Engineering 2013年第3期10卷 316-328页

作者： Tuyen Quang Le Tien Van Truong Hieu Trung Tran Soo Hyung Park jin Hwan Ko Hoon cheol Park Kwang Joon Yoon Doyoung Byun Korea Institute of Ocean Science and Technology PO Box 29 Ansan 425-600 Republic of Korea Department of Aerospace and Information Engineering Konkuk University Seoul Republic of Korea Department of Advanced Technology Fusion Konkuk University Seoul Republic of Korea Department of Mechanical Engineering Sungkyunkwan University Suwon440-746 Republic of Korea

Aerodynamic characteristic of the beetle, Trypoxylus dichotornus, which has a pair of elytra （forewings） and hind wings, is numerically investigated. Based on the experimental results of wing kinematics, two-dimensional （2D） and three-dimensional （3D） computational fluid dynamic simulations were carried out to reveal aerodynamic performance of the hind wing. The roles of the spiral Leading Edge Vortex （LEV） and the spanwise flow were clarified by comparing 2D and 3D simulations. Mainly due to pitching down of chord line during downstroke in highly inclined stroke plane, relatively high averaged thrust was produced in the free forward flight of the beetle. The effects of the local corrugation and the camber variation were also investigated for the beetle＇s hind wings. Our results show that the camber variation plays a significant role in improving both lift and thrust in the flapping. On the other hand, the local corrugation pattern has no significant effect on the aerodynamic force due to large angle of attack during flapping.

关键词： beetle flight forward flight three-dimensional simulation corrugation effect time-camber variation

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