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tRNA^(Glu)-derived fragments from embryonic extracellular vesicles modulate bovine embryo hatching

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1559-1566页

作者： Yuan Fan Krishna Chaitanya Pavani Katrien Smits ann van Soom Luc Peelman Department of Veterinary and Biosciences Faculty of Veterinary MedicineGhent UniversityHeidestraat 199820 MerelbekeBelgium Department of Internal Medicine Reproduction and Population MedicineFaculty of Veterinary MedicineGhent UniversitySalisburylaan 1339820 MerelbekeBelgium Department for Reproductive Medicine Ghent University HospitalCorneel Heymanslaan 109000 GhentBelgium

Transfer RNA-derived small RNAs(tsRNAs)have been shown to be involved in early embryo development and repression of endogenous retroelements in embryos and stem ***,it is unknown whether tsRNAs also regulate embryo *** this study,we mined the sequencing data of a previous experiment in which we demonstrated that the microRNA(miRNA)cargo of preimplantation embryonic extracellular vesicles(EVs)influences embryo *** thus profiled the tsRNA cargo of EVs secreted by blastocysts and *** majority of tsRNAs was identified as tRNA halves originating from the 5'ends of *** the 148 differentially expressed tsRNAs,the 19 nt tRNA fragment(tRF)tDR-14:32-Glu-CTC-1 was found to be significantly up-regulated in EVs derived from ***-qPCR assays confirmed its significant up-regulation in non-blastocyst embryos and their conditioned medium compared to the blastocyst group(P<0.05).Inhibition of tDR-14:32-Glu-CTC-1 by supplementing antagomirs to the conditioned medium improved embryo hatching(P<0.05).Transcriptomic analysis of embryos treated with tDR-14:32-Glu-CTC-1 antagomirs further showed differential expression of genes that are associated with embryo hatching and *** summary,tDR-14:32-Glu-CTC-1 is up-regulated in non-blastocyst embryos and their secretions,and inhibition of tDR-14:32-Glu-CTC-1 promotes embryo hatching,while influencing embryo implantation-related genes and *** results indicate that embryonic EVs containing specific tRFs may regulate preimplantation embryo development.

关键词： Embryo Extracellular vesicles Hatching tRNA fragments ts RNAs

Homing and migration of mesenchymal stromal cells: How to improve the efficacy of cell therapy?

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World Journal of Stem Cells 2016年第3期8卷 73-87页

作者： ann De Becker Ivan van Riet Department Clinical HematologyStem Cell Laboratory Universitair Ziekenhuis Brussel Vrije Universiteit Brussel (VUB)

Mesenchymal stromal cells(MSCs) are currently being investigated for use in a wide variety of clinical applications. For most of these applications, systemic delivery of the cells is preferred. However, this requires the homing and migration of MSCs to a target tissue. Although MSC hominghas been described, this process does not appear to be highly efficacious because only a few cells reach the target tissue and remain there after systemic administration. This has been ascribed to low expression levels of homing molecules, the loss of expression of such molecules during expansion, and the heterogeneity of MSCs in cultures and MSC culture protocols. To overcome these limitations, different methods to improve the homing capacity of MSCs have been examined. Here, we review the current understanding of MSC homing, with a particular focus on homing to bone marrow. In addition, we summarize the strategies that have been developed to improve this process. A better understanding of MSC biology, MSC migration and homing mechanisms will allow us to prepare MSCs with optimal homing capacities. The efficacy of therapeutic applications is dependent on efficient delivery of the cells and can, therefore, only benefit from better insights into the homing mechanisms.

关键词： Mesenchymal stromal cells Homing Bone marrow Homing receptors Extravasation

Multisensory mechanisms of gait and balance in Parkinson’s disease:an integrative review

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Neural Regeneration Research 2025年第1期20卷 82-92页

作者： Stiven Roytman Rebecca Paalanen Giulia Carli Uros Marusic Prabesh Kanel Teus van Laar Nico I.Bohnen Department of Radiology University of MichiganAnn ArborMIUSA Department of Neurology University of MichiganAnn ArborMIUSA Morris K.Udall Center of Excellence for Parkinson’s Disease Research University of MichiganAnn ArborMIUSA Institute for Kinesiology Research Science and Research Centre KoperKoperSlovenia Department of Health Sciences Alma Mater Europaea–ECMMariborSlovenia Parkinson’s Foundation Research Center of Excellence University of MichiganAnn ArborMIUSA Department of Neurology University of GroningenUniversity Medical Center GroningenGroningenThe Netherlands Neurology Service and GRECC VA Ann Arbor Healthcare SystemAnn ArborMIUSA

Understanding the neural underpinning of human gait and balance is one of the most pertinent challenges for 21st-century translational neuroscience due to the profound impact that falls and mobility disturbances have on our aging *** and gait control does not happen automatically,as previously believed,but rather requires continuous involvement of central nervous *** effectively exert control over the body,the brain must integrate multiple streams of sensory information,including visual,vestibular,and somatosensory *** mechanisms which underpin the integration of these multisensory signals are the principal topic of the present *** multisensory integration theories focus on how failure of cognitive processes thought to be involved in multisensory integration leads to falls in older *** emphasis,however,has been placed on specific contributions of individual sensory modalities to multisensory integration processes and cross-modal interactions that occur between the sensory modalities in relation to gait and *** the present work,we review the contributions of somatosensory,visual,and vestibular modalities,along with their multisensory intersections to gait and balance in older adults and patients with Parkinson’s *** also review evidence of vestibular contributions to multisensory temporal binding windows,previously shown to be highly pertinent to fall risk in older ***,we relate multisensory vestibular mechanisms to potential neural substrates,both at the level of neurobiology(concerning positron emission tomography imaging)and at the level of electrophysiology(concerning electroencephalography).We hope that this integrative review,drawing influence across multiple subdisciplines of neuroscience,paves the way for novel research directions and therapeutic neuromodulatory approaches,to improve the lives of older adults and patients with neurodegenerative diseases.

关键词： aging balance encephalography functional magnetic resonance imaging gait multisensory integration Parkinson’s disease positron emission tomography somatosensory vestibular visual

高效液相色谱法测定生物样品中3PO的方法建立与验证（英文）

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药物分析杂志 2013年第11期33卷 1873-1876,1886页

作者： Yemarshet K.Gebreyohannes 王旭 Getu Kahsay Weldu Erwin Adams ann van schepdael 荷语天主教鲁汶大学药学院药物分析实验室

目的:建立并验证用于大鼠血清、脑脊液、大脑和脊髓中3-(3-吡啶)-1-(4-吡啶)-2-丙烯-1-酮(3PO)含量测定的HPLC-UV法。方法:样品处理使用蛋白沉淀的方法;色谱条件:色谱柱为Chromolith RP-18e(100 mm×4.6 mm),流动相A为乙腈-水(2.5∶97.5... 详细信息

目的:建立并验证用于大鼠血清、脑脊液、大脑和脊髓中3-(3-吡啶)-1-(4-吡啶)-2-丙烯-1-酮(3PO)含量测定的HPLC-UV法。方法:样品处理使用蛋白沉淀的方法;色谱条件:色谱柱为Chromolith RP-18e(100 mm×4.6 mm),流动相A为乙腈-水(2.5∶97.5,v/v)添加0.1%TFA,流动相B为乙腈添加0.1%TFA,梯度洗脱[0～2.3 min,0%B→100%B(线性梯度);2.3～2.6 min,100%B(等度);2.6～2.7 min,100%B→0%B(线性梯度);2.7～5 min,0%B(等度)],流速2 mL·min-1,检测波长304 nm,进样量10μL。结果:大鼠的血清、脑脊液、大脑和脊髓中内源性物质不干扰3PO的测定,在上述4种基质中,该方法的检测限分别为0.2、0.1、0.1、0.1μg·mL-1;定量限分别为0.8、0.2、0.5、0.5μg·mL-1;线性范围分别为3～150、0.3～150、0.9～150、0.6～150μg·mL-1,相关系数均大于0.999;高、低浓度的回收率均在79.9%～100.3%的范围内;方法重复性(n=5)的RSD均大于0.9%。结论:建立的方法简单,线性范围宽,且选择性、耐用性均佳。

关键词：高效液相色谱生物分析 3PO 3-(3-吡啶)-1-(4-吡啶)-2-丙烯-1-酮

Fatigue in children and adolescents with inflammatory bowel disease

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World Journal of Gastroenterology 2019年第5期25卷 632-643页

作者： Els van de Vijver ann van Gils Laura Beckers Yannick van Driessche Nicolette Dorien Moes Patrick Ferry van Rheenen Department of Paediatric Gastroenterology Hepatology and Nutrition Antwerp University Hospital Department of Anesthesiology Antwerp University Hospital Family Medicine Department of Paediatric Gastroenterology Hepatology and Nutrition University Medical Center Groningen

AIM To identify factors other than active disease and anemia that contribute to fatigue in pediatric inflammatory bowel disease(IBD).METHODS We performed an electronic search in Medline and EMBASE from their inception to May 2017 using the search term "fatigue" or the related keywords "physical impairment" and "inflammatory bowel disease" with the filter "child"(age 0-18 years). Cross-sectional and case-control studies were included. We restricted our search to studies published in English. We used the PRISMA checklist and flow diagram. Duplicate articles were manually deleted in End Note. To identify further relevant studies, we checked the reference lists of the selected *** We identified 149 papers, of which 19 were retrieved for full text review. Eleven studies were subsequently excluded because fatigue was not evaluated as an outcome measure. Eight papers focused on the desired topic and were discussed in the final analysis. A lack of uniformity of outcome measures made the pooling of data impossible. In all but one study, questionnaires were used to evaluate fatigue. In the remaining study, an accelerometer was used to measure daily activities, sleeping time and their relationships with fatigue in a more quantifiable manner. Adolescents with IBD are significantly more fatigued than healthy controls. In addition to active disease, increased anxiety or depression and disturbed family relationships were frequently reported predictors of fatigue. Quantitative measurement of physical activity in patients with Crohn's disease showed a reduction in the number of steps per day, and patients with ulcerative colitis had a shorter duration of physical activity during the *** Fatigue in pediatric IBD is related to a combination of biological, functional and behavioral factors, which should all be taken into account when managing fatigue.

关键词： Adolescents Children Fatigue Inflammatory bowel disease Physical impairment Sleep

Adaptation of forest management to climate change as perceived by forest owners and managers in Belgium

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Forest Ecosystems 2017年第1期4卷 22-32页

作者： Rita Sousa-Silva Quentin Ponette Kris Verheyen ann van Herzele Bart Muys Ku Leuven Department of Earth and Environmental Sciences Celestijnenlaan 200E Box 24113001 Leuven Belgium Earth and Life Institute Environmental Sciences Universite catholique de Louvain Croix du Sud 2 Box L7.05.091348 Louvain-la-Neuve Belgium. Forest & Nature Lab Ghent University Geraardsbergsesteenweg 267 9090 Melle-Gontrode Belgium. Nature & Society Research Group Research Institute for Nature and Forest (INBO) Kliniekstraat 25 B-1070 Brussels Belgium. Forest Institute (EFIMED) Sant Pau Historical Site Sant Leopold Pavilion Carrer Sant Antoni Maria Claret 167 08025 Barcelona Spain.

Background： Climate change is likely to cause significant modifications in forests. Rising to this challenge may require adaptation of forest management, and therefore should trigger proactive measures by forest managers, but it is unclear to what extent this is already happening. Methods： The survey carried out in this research assesses how forest stakeholders in Belgium perceive the role of their forest management in the context of climate change and the impediments that limit their ability to prepare and respond to these changes. Results： Respondents indicated strong awareness of the changing climate, with more than two-thirds （71%） expressing concern about the impacts of climate change on their forests. However, less than one-third of the respondents （32 %） reported modifying their management practices motivated by climate change. Among the major constraints limiting their climate red,ted ~ctions, lack of ic^formation was considered the most important for managers of both public and private forests. Conclusions： Knowledge transfer is an essential condition for research to lead to innovation. Improving the communication and demonstration of possible solutions for climate change adaptation is therefore likely to be the most effective strategy for increasing their adoption.

关键词： Adaptation Adoption Belief Stakeholders Internet survey Logistic regression

Temperature and Precipitation Suitability Evaluation for the Winter Wheat and Summer Maize Cropping System in the Huang-Huai-Hai Plain of China

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Agricultural Sciences in China 2011年第2期10卷 275-288页

作者： Nguyen Thanh Tuan QIU Jian-jun ann Verdoodt LI Hu Eric van Ranst Vietnam National Museum of Nature Vietnamese Academy of Science and TechnologyHa Noi 0000Vietnam Key Laboratory of Resources Remote Sensing and Digital Agriculture Ministry of Agriculture/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural Sciences（CAAS）Beijing 100081P.R.China Laboratory of Soil Science Department of Geology and Soil Science（WE13）Ghent UniversityB-9000 GhentBelgium

The Huang-Huai-Hai Plain is the most important winter wheat and maize production region in *** response to the increasing population pressure,the Chinese authorities strongly invested in improving the irrigation systems and increasing ground and surface water exploitation within the plain to increase the crop *** resulted in a reduction of water resource availability and in turn indirectly affected the suitability of various agricultural ecosystems in the *** main purpose of this study was to review the suitability of temperature and precipitation for the winter wheat and summer maize cropping system in the Huang-Huai-Hai Plain,in order to provide a preliminary irrigation *** analysis provides a first attempt to enhance the water resource management as well as to increase the water use *** this aim,a GIS-based multicriteria analysis procedure has been developed consisting of（1） definition of objectives（evaluated entities） and database building;（2） definition of evaluation criteria;（3） standardization of the criteria;（4） combination of the criteria;（5） classification of the objectives;and（6） integration of the *** land suitability classification maps were transformed into maps of required irrigation amounts for each growing stage of the winter wheat and summer maize cropping *** study also exemplified the limitations and proposed future research activities that will improve the detail and accuracy of the evaluation results.

关键词： GIS multicriteria Huang-Huai-Hai Plain precipitation suitability irrigation

Regeneration of dendritic cells in aged mice

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Cellular & Molecular Immunology 2010年第2期7卷 108-115页

作者： Serani LH van Dommelen Alexandra Rizzitelli ann Chidgey Richard Boyd Ken Shortman Li Wu The Walter and Eliza Hall Institute of Medical Research Parkville Vic. Australia Monash Immunology and Stem Cell Laboratories Monash University Clayton Vic. Australia Current address: Department of Biochemistry and Molecular Biology Monash University Clayton Vic. Australia

Age-related thymic involution causes a decreased output of thymocytes from the thymus, thereby resulting in impairment of T cell-mediated immunity. While alterations in the T cell and non-haematopoietic stromal compartments have been described, the effects of thymic involution on thymic dendritic cells （DC） are not clearly known. Thymic DC play an essential role in shaping T cell-mediated immune responses by deleting self-reactive thymocytes to establish central tolerance and by inducing regulatory T-cell （Treg） development. It is therefore important to assess the prevalence of and alterations to thymic DC with age, as this may impact on their function. We assessed the numbers and proportions of the three distinct subsets of thymic DC in ageing mice, and showed that these subsets are differentially regulated. This is expected as thymic DC subsets have different origins of development. We further assessed the responses of thymic DC in a regenerative environment, such as that induced by sex-steroid ablation （SSA）, and clearly showed that, consistent with global thymus regrowth, all three DC populations increased in numbers and regained their relative proportions to thymocytes after an initial lag period. These findings are important for the clinical translation of thymic regenerative approaches, and indicate that SSA facilitates the maintenance of critical processes such as negative selection and Treg induction through promoting thymic DC regeneration.

关键词： ageing dendritic cells thymic regeneration

Effectiveness of psychosocial interventions for pediatric patients with scoliosis:a systematic review

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World Journal of Pediatric Surgery 2023年第2期6卷 63-69页

作者： Maike van Niekerk ann Richey John Vorhies Connie Wong Kali Tileston Pediatric Orthopaedic Surgery Stanford UniversityStanfordCaliforniaUSA Lane Medical Library Stanford UniversityStanfordCaliforniaUSA

Background Pediatric patients affected by scoliosis have complex psychological and social care needs,and may benefit from psychosocial *** therefore aimed to summarize evidence of the efficacy of psychosocial interventions for this patient *** Literature was identified by searching Medline,PsycINFO,Embase,EBSCO Cumulated Index to Nursing and Allied Health Literature(CINAHL),and Cochrane Central Register of Controlled Trials(CENTRAL)from database inception to 20 March *** that evaluated the effectiveness of psychosocial interventions for pediatric patients diagnosed with scoliosis and reported at least one quantitative outcome were *** eligibility,data extraction,and quality assessment(using the Cochrane Collaboration's Risk of Bias Tool and Methodological Index for Non-Randomized Studies)were performed by two independent *** are presented using narrative *** We identified ten studies,all of which focused on adolescent idiopathic *** included a total of 1007 participants,most of whom were *** studies focused on patients undergoing bracing,six on patients undergoing spinal surgery,and one on patients *** compliance monitoring and counseling were found to significantly improve brace compliance quality and *** mental healthcare delivery by nurses after spinal surgery was similarly found to improve *** studies examined the efficacy of brief educational interventions;most did not report clear evidence of their *** methodological quality of studies was often unclear due to limitations in articles'reporting *** Research on the efficacy of psychosocial interventions for pediatric patients with scoliosis is limited,with interventions involving frequent patient-provider interactions showing the most *** clinical and research efforts should focus on developing and testing psychosocial interventions for

关键词： patients scoliosis nurse