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动物免疫学线上线下混合式教学融入课程思政的方法及实践

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中国免疫学杂志 2024年第3期40卷 621-625,635页

作者：李海花李留安胡烨丁巧玲赵瑞利邵华天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室天津300384

目的:在动物免疫学课程中真正实现思想政治教育和专业教育的深度融合,有效提升学生的综合素质。方法:实验采用了动物免疫学线上线下混合式教学融入课程思政的教学方法,对课程设计理念、教学目标、教学设计和教学实施进行研究,并且从成... 详细信息

目的:在动物免疫学课程中真正实现思想政治教育和专业教育的深度融合,有效提升学生的综合素质。方法:实验采用了动物免疫学线上线下混合式教学融入课程思政的教学方法,对课程设计理念、教学目标、教学设计和教学实施进行研究,并且从成绩和思政效果对教学成效进行评价。结果:表明该新型教学模式能够激发学生的学习兴趣,有助于提高学生考试成绩和综合能力的培养。结论:该方法教学效果良好,实现了立德树人的根本任务。

关键词：动物免疫学线上线下教学课程思政教育内涵式发展课程设计学生素质教育

Synthesis,characterization and cell imaging properties of rare earth compounds based on hydroxamate ligand

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Journal of Rare Earths 2018年第4期36卷 418-423页

作者： Linyan Yang Yanping Zhang Liwei Hu Yunhe Zong ruili zhao Tianming Jin Wen Gu College of Animal Science and Veterinary Medicine Tianjin Agricultural UniversityTianjin 300384China Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources Guangxi Normal UniversityGuilin 541004China College of Chemistry Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)Nankai UniversityTianjin 300071China Key Laboratory of Advanced Energy Materials Chemistry(KLAEMC) Nankai UniversityTianjin 300071China

Six binuclear rare earth compounds were synthesized through the ligand of 4-Bromo-N-hydroxy-Nmethyl-benzamide（sH_2 bha）. Compounds 1-3 are isomorphous, and compound 1 is crystallized in the monoclinic system with the P2_1/c space group. Compounds 4 and 5 are isomorphous, and compound 4 is crystallized in the triclinic system with the P-1 space group. Compound 6 is crystallized in the triclinic system with the P-1 space group. The compounds were characterized by thermogravimetric analysis（TGA） and IR spectroscopy. The fluorescence spectrum in the visible area shows characteristic peaks of Eu, Tb, and Dy compounds. The efficiency of Eu compound on the viability of PC3 cells was assessed using CCK8 assays. From the CCK8 results, ionic concentration may have a great effect on PC3 cells＇ proliferation. Eu compound shows red fluorescence in the cytoplasm of PC3 cell. As for Eu compound, it might have potential application in cell imaging technology.

关键词： Rare earth Fluorescence Cell imaging

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基于节气文化元素的户外景观灯具设计研究

工业设计 2024年第8期 129-132页

作者：王涣苒张玲玉赵瑞丽四川轻化工大学机械工程学院宜宾644000

户外景观灯具具有亮化和美化环境的功能,研究将户外景观灯具作为文化呈现的新载体,探索融入节气文化元素进行景观灯具的创新设计。通过对贯穿中国人民生活始终的二十四节气的历史习俗和文化内涵进行深入研究,分析其与户外灯具设计的关... 详细信息

户外景观灯具具有亮化和美化环境的功能,研究将户外景观灯具作为文化呈现的新载体,探索融入节气文化元素进行景观灯具的创新设计。通过对贯穿中国人民生活始终的二十四节气的历史习俗和文化内涵进行深入研究,分析其与户外灯具设计的关联性。通过分析文化层次进行设计元素提取,将表层可视化与深层可视化相结合,针对特定节气主题进行系列美陈灯具的设计实践,提出节气文化特色的组合景观灯具设计方案。将中国传统文化中的二十四节气文化应用于户外景观灯具的创新设计,可赋予户外景观灯具独特的传统文化和美学内涵,满足人们对于文化认同、情感共鸣的需求,促进夜间经济繁荣。

关键词：工业设计户外景观灯二十四节气文化创意设计传统文化

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情感化设计在儿童滑板车中的应用研究

设计 2024年第15期37卷 124-128页

作者：赵瑞丽张玲玉王涣苒四川轻化工大学机械工程学院

探究情感化设计理论在儿童滑板车设计中的应用方法,拓展情感化设计理论在儿童产品设计领域的应用。通过分析儿童的生理和心理特征,结合情感化设计理论在设计实践的应用研究,从本能层、行为层和反思层3个维度,提出儿童滑板车情感化设计策... 详细信息

探究情感化设计理论在儿童滑板车设计中的应用方法,拓展情感化设计理论在儿童产品设计领域的应用。通过分析儿童的生理和心理特征,结合情感化设计理论在设计实践的应用研究,从本能层、行为层和反思层3个维度,提出儿童滑板车情感化设计策略,进行设计实践。设计出一辆符合儿童情感需求的太空主题儿童滑板车。情感化设计理论和方法可为其他儿童滑板车设计提供新的设计思路,提高产品的趣味性,增强儿童的运动积极性,促进身心健康发展。

关键词：儿童滑板车情感化设计情感需求运动体验设计策略

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猪链球菌靶向DC重组蛋白DC3pep-SDZ对斑马鱼的免疫保护性分析

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中国兽医杂志 2024年第4期60卷 35-42页

作者：于恩远荣睿璠曾君赵瑞利郭海悦张东超胡烨李颖王心如天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室天津西青300392 天津市动物疫病预防控制中心天津北辰300402

为了探究本课题组前期构建的由猪链球菌(SS)的分泌型DNA核酸酶A(SsnA)、二氢硫辛酰胺脱氢酶(DLDH)和高亲和力锌摄取系统蛋白(ZnuA)3个毒力因子的优势细胞表位和树突状细胞靶向肽(DC3pep)串联而成的重组蛋白DC3pep-SDZ(SsnA-DLDH-ZnuA)... 详细信息

为了探究本课题组前期构建的由猪链球菌(SS)的分泌型DNA核酸酶A(SsnA)、二氢硫辛酰胺脱氢酶(DLDH)和高亲和力锌摄取系统蛋白(ZnuA)3个毒力因子的优势细胞表位和树突状细胞靶向肽(DC3pep)串联而成的重组蛋白DC3pep-SDZ(SsnA-DLDH-ZnuA)对致病型SS攻击斑马鱼的免疫保护效果,本试验表达和纯化重组蛋白DC3pep-SDZ和SDZ,分别免疫AB系野生型斑马鱼,攻击致病型2、3和9型SS(SS2、SS3和SS9),计算存活率,采用苏木精-伊红(H.E.)染色法观察斑马鱼脑组织病理变化,实时荧光定量PCR(RT-qPCR)分析SS3感染斑马鱼后脑组织炎性细胞因子表达量变化。结果显示,诱导表达的重组蛋白DC3pep-SDZ和SDZ分子质量分别为44和42 kDa,2个蛋白均以可溶的形式表达。SS2、SS3和SS9攻击斑马鱼后,重组蛋白DC3pep-SDZ组和重组蛋白SDZ组斑马鱼存活率分别为50.0%、65.0%和60.0%,45.0%、47.3%和45.0%,两组间差异不显著(P>0.05),但与PBS对照组相比差异均极其显著(P<0.001)。H.E.染色镜检结果显示,重组蛋白DC3pep-SDZ组的斑马鱼被3种分型SS攻击后脑组织均无明显病理变化。RT-qPCR结果显示,重组蛋白DC3pep-SDZ组促炎细胞因子:与PBS对照组相比较IL-6、TNF-α和IFN-γmRNA表达量分别极显著(P<0.01)、极其显著(P<0.001)和极显著(P<0.01)下调,与重组蛋白SDZ组相比较IL-6和TNF-αmRNA表达量分别极显著(P<0.01)和显著下调(P<0.05);重组蛋白DC3pep-SDZ组抗炎性细胞因子:与PBS对照组相比较IL-10和TGF-β1 mRNA表达量分别极显著(P<0.01)和极其显著(P<0.001)上调,与重组蛋白SDZ组相比较TGF-β1 mRNA表达量显著上调(P<0.05)。结果表明,重组蛋白DC3pep-SDZ较SDZ具有更好的免疫原性,能产生SS2、SS3和SS9交叉免疫保护作用,同时还能够有效缓解斑马鱼感染SS3后的脑部炎症。

关键词：猪链球菌斑马鱼表位蛋白 DC3pep-SDZ 免疫保护

Acupotomy inhibits aberrant formation of subchondral bone through regulating osteoprotegerin/receptor activator of nuclear factor-κB ligand pathway in rabbits with knee osteoarthritis induced by modified Videman method

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Journal of Traditional Chinese Medicine 2022年第3期42卷 389-399页

作者： QIN Luxue GUO Changqing zhao ruili WANG Tong WANG Junmei GUO Yan ZHANG Wei HU Tingyao CHEN Xilin ZHANG Qian ZHANG Dian XU Yue School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese MedicineBeijing 100029China the First People's Hospital of Dongcheng District Beijing 100050China Beijing Hospital of Traditional Chinese Medicine Capital Medical UniversityBeijing 100010China the Third Affiliated Hospital of Beijing Universality of Chinese Medicine Beijing 100029China

OBJECTIVE:To investigate the effects of acupotomy on inhibiting abnormal formation of subchondral bone in rabbits with knee osteoarthritis(KOA).METHODS:A total of 24 New Zealand rabbits were randomly divided into four groups of 6 rabbits each[control,model,electroacupuncture(EA)and acupotomy].Eighteen KOA model rabbits were established using a modified Videman *** in EA and acupotomy groups received the intervention for 3 ***,the cartilage and subchondral bone unit were obtained and the histomorphological changes were ***(OPG)and receptor activator of nuclear factor-κB ligand(RANKL)in subchondral bone were evaluated by Western blotting,real-time polymerase chain reaction and ***:Compared with the model group,both the acupotomy and EA groups showed a significant decrease in the Lequesne index(both P<0.01)and Mankin score(P<0.01,<0.05).In addition,both EA and acupotomy groups had a higher expression of total articular cartilage(TAC)(P<0.05,<0.01)and lower expression of articular calcified cartilage(ACC)/TAC(P<0.05,<0.05)compared with the model *** thickness of the subchondral bone plate in EA and acupotomy groups were decreased(both P<0.01)compared to the model ***,trabecular bone volume(BV/TV),protein and relative expression of OPG and the ratio of OPG/RANKL in the subchondral bone of acupotomy group were decreased statistically significant,while these parameters were not significantly changed in the EA group compared with the model ***:In the rabbit model of KOA,acupotomy inhibits aberrant formation of subchondral bone by suppressing OPG/RANKL ratio as a potential therapy for KOA.

关键词： acupuncture therapy osteogenesis osteoarthritis,knee subchondral bone

Effect of acupuncture at Renying(ST 9) on gene expression profile of hypothalamus in spontaneously hypertensive rats

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Journal of Traditional Chinese Medicine 2018年第2期38卷 227-241页

作者： Guo Yan Lu Juan Liang Jingrong zhao ruili Xu Jing Zhang Wei Park Kibeum Zhu Shipeng Chen Huan Ma Liangxiao Department of Acupuncture and Moxibustion First Hospital Affiliated to Tianjin College of Traditional Chinese Medicine College of Acupuncture-Moxibustion and Tuina Beijing University of Chinese Medicine College of Acupuncture-Moxibustion and Tuina Second School of Clinical Medical of Nanjing University of Traditional Chinese Medicine Department of Acupuncture and Moxibustion Jiangsu Province Hospital

OBJECTIVE: To investigate changes in gene expression profiles in the hypothalamus related to the effects of acupuncture at the Renying(ST 9) acupoint in spontaneously hypertensive(SH) ***: We randomly divided 18 SH rats into Renying(ST 9) group and model control group, 9 body weight-matched Wistar-Kyoto rats were used as blank controls. Acupuncture was performed manually for 20-min daily over 28 d in the Renying(ST 9) group. Rat Gene 2.0 array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction(RT-PCR) ***: The different groups exhibited differential gene expression: compared with the blank control group, 48 genes were up-regulated and 91 genes were down-regulated in the model group;compared with the model group, 79 genes were up-regulated and 80 genes were down-regulated in Renying(ST 9) group. The RT-PCR results of the key genes including Chi3 l1, Ephx2, Klk1, 5-HT1 A and Cbs were consistent with that of gene chip ***: Acupuncture at Renying(ST 9)could significantly lower the blood pressure of SH rats and affect their hypothalamic gene expression profile. Genes associated with the contraction of vascular smooth muscle and the regulation of inflammation, neurotransmitters may be involved in acupuncture's antihypertensive mechanism.

关键词： Essential hypertension Point ST 9(Re-nying) Oligonucleotide array sequence analysis Hypothalamus

Characteristics of the multiple replicon plasmid IncX1-X1 in multidrug-resistant Escherichia coli from Malayan pangolin(Manis javanica)

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Integrative Zoology 2023年第2期18卷 289-298页

作者： Fang JI Shelan LIU Xue WANG Jianan zhao Jiayue ZHU Jianchun YANG Chenglin ZHANG Zhongxin JIA ruili zhao Guocheng HU Jing WANG Jianhua QIN Gang LI Bin WU Chengmin WANG Guangdong Key Laboratory of Animal Conservation and Resource Utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationInstitute of ZoologyGuangdong Academy of ScienceGuangzhouChina Department of Infectious Diseases Zhejiang Center of Disease Control and PreventionHangzhouZhejiangChina School of Bioengineering East China University of Science and TechnologyShanghaiChina Beijing Key Laboratory of Captive Wildlife Technologies Beijing ZooBeijingChina College of Animal Science and Veterinary Medicine Tianjin Agricultural UniversityTianjinChina South China Institute of Environmental Sciences Ministry of Ecology and EnvironmentGuangzhouChina Department of Infectious Diseases Hangzhou Center of Disease Control and PreventionHangzhouChina College of Veterinary Medicine Agricultural University of HebeiBaodingChina

Potential zoonotic pathogens may be transmitted from wildlife to humans through the illegal wild meat trade,which has become a pressing ***,research on the antimicrobial resistance genes(ARGs)of Malayan pangolin(Manis javanica)intestinal bacteria is ***,multidrug-resistant Escherichia coli M172-1(ST354)isolated from Malayan pangolin feces in 2019 was found to be resistant to 13 *** analysis revealed 4 plas-mids,namely,pM172-1.1,pM172-1.2,pM172-1.3,and pM172-1.4,in the *** pM172-1.2,pM172-1.3,and pM172-1.4 plasmids carried ARGs,namely,IncHI2-HI2A,IncX1-X1,and IncX1,***172-1.3 and pM172-1.4 contained intact IntI1 integrons(Is26/IntI1/arr2/cmlA5/blaOXA-10/ant(3′′)-IIA/dfrA14/Is26).No-tably,pM172-1.3 resulted from the fusion of 2 pM172-1.4 copies and carried many more *** addition to pM172-1.3 from the same host,other drug-resistant bacteria(*** M159-1(ST48),*** S171-1(ST206),and Klebsiella pneumoniae S174-1(ST2354))in the same Malayan pangolin fecal samples also carried 3 plasmids with 100%gene coverage of pM172-1.4 and 99.98%***,ARGs in IncX1 might spread in the intestinalﬂora of Malayan pangolin and between species via the illegal food chain,posing a potential threat to public health and safety.

关键词： ARG Manis javanica multiple-replicon plasmid IncX1-X1 plasmid IncX1

Thylakoid engineered M2 macrophage for sonodynamic effect promoted cell therapy of early atherosclerosis

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Nano Research 2024年第4期17卷 2919-2928页

作者： Guanghao Wu Changwen Mu Qianru zhao Yao Lei Ran Cheng Weidong Nie Jiamin Qu Yuping Dong ruili Yang Haiyan Xie State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesChemical Biology CenterPeking UniversityBeijing 100191China School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China School of Life Science Beijing Institute of TechnologyBeijing 100081China School of Stomatology Peking UniversityBeijing 100081China

Atherosclerosis is the most common cause of cardiovascular diseases that contribute to the major morbidity worldwide,but still lacking of effective treatment ***,a hybrid cell is constructed for the sonodynamic effect promoted cell therapy of early atherosclerosis by fusing M2 macrophages with thylakoid(TK)*** systemic administration,the obtained TK-M2 actively accumulates in the early atherosclerotic plaques,wherein M2 macrophages relieve the cholesterol accumulation and the inflammation in the foam ***,the TK membranes decorated on the M2 macrophages exhibit both type I and type II sonodynamic effects under ultrasound(US)activation,inducing the direct apoptosis of foam *** cooperation of M2 and TK leads to significant outcome in eliminating atherosclerotic plaques without obvious side-effects,providing a new avenue for atherosclerosis treatment.

关键词： atherosclerosis thylakoid ultrasound M2 macrophage