Effect of acupuncture at Renying(ST 9) on gene expression profile of hypothalamus in spontaneously hypertensive rats

作     者：Guo Yan Lu Juan Liang Jingrong Zhao Ruili Xu Jing Zhang Wei Park Kibeum Zhu Shipeng Chen Huan Ma Liangxiao 

作者机构：Department of Acupuncture and MoxibustionFirst Hospital Affiliated to Tianjin College of Traditional Chinese Medicine College of Acupuncture-Moxibustion and TuinaBeijing University of Chinese Medicine College of Acupuncture-Moxibustion and TuinaSecond School of Clinical Medical of Nanjing University of Traditional Chinese Medicine Department of Acupuncture and MoxibustionJiangsu Province Hospital 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2018年第38卷第2期

页      面：227-241页

核心收录：

学科分类：1005[医学-中医学] 1002[医学-临床医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：Supported by a Grant from the State Administration of Traditional Chinese Medicine Industry Subject:the Study and Application of Acupuncture in the Prevention and Treatment of Stroke of the Primary Hypertension(No.201507001-08) 

主　　题：Essential hypertension Point ST 9(Re-nying) Oligonucleotide array sequence analysis Hypothalamus 

摘      要：OBJECTIVE: To investigate changes in gene expression profiles in the hypothalamus related to the effects of acupuncture at the Renying(ST 9) acupoint in spontaneously hypertensive(SH) ***: We randomly divided 18 SH rats into Renying(ST 9) group and model control group, 9 body weight-matched Wistar-Kyoto rats were used as blank controls. Acupuncture was performed manually for 20-min daily over 28 d in the Renying(ST 9) group. Rat Gene 2.0 array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction(RT-PCR) ***: The different groups exhibited differential gene expression: compared with the blank control group, 48 genes were up-regulated and 91 genes were down-regulated in the model group;compared with the model group, 79 genes were up-regulated and 80 genes were down-regulated in Renying(ST 9) group. The RT-PCR results of the key genes including Chi3 l1, Ephx2, Klk1, 5-HT1 A and Cbs were consistent with that of gene chip ***: Acupuncture at Renying(ST 9)could significantly lower the blood pressure of SH rats and affect their hypothalamic gene expression profile. Genes associated with the contraction of vascular smooth muscle and the regulation of inflammation, neurotransmitters may be involved in acupuncture s antihypertensive mechanism.