Characteristics of the multiple replicon plasmid IncX1-X1 in multidrug-resistant Escherichia coli from Malayan pangolin(Manis javanica)

作     者：Fang JI Shelan LIU Xue WANG Jianan ZHAO Jiayue ZHU Jianchun YANG Chenglin ZHANG Zhongxin JIA Ruili ZHAO Guocheng HU Jing WANG Jianhua QIN Gang LI Bin WU Chengmin WANG 

作者机构：Guangdong Key Laboratory of Animal Conservation and Resource UtilizationGuangdong Public Laboratory of Wild Animal Conservation and UtilizationInstitute of ZoologyGuangdong Academy of ScienceGuangzhouChina Department of Infectious DiseasesZhejiang Center of Disease Control and PreventionHangzhouZhejiangChina School of BioengineeringEast China University of Science and TechnologyShanghaiChina Beijing Key Laboratory of Captive Wildlife TechnologiesBeijing ZooBeijingChina College of Animal Science and Veterinary MedicineTianjin Agricultural UniversityTianjinChina South China Institute of Environmental SciencesMinistry of Ecology and EnvironmentGuangzhouChina Department of Infectious DiseasesHangzhou Center of Disease Control and PreventionHangzhouChina College of Veterinary MedicineAgricultural University of HebeiBaodingChina 

出 版 物：《Integrative Zoology》 (整合动物学（英文版）)

年 卷 期：2023年第18卷第2期

页      面：289-298页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the Introduction of Leading Talents Program of the Guangdong Academy of Sciences(No.2016GDASRC-0205) the Open project of Beijing Key Laboratory of captive wildlife technology in Beijing Zoo(ZDK202105) 

主　　题：ARG Manis javanica multiple-replicon plasmid IncX1-X1 plasmid IncX1 

摘      要：Potential zoonotic pathogens may be transmitted from wildlife to humans through the illegal wild meat trade,which has become a pressing ***,research on the antimicrobial resistance genes(ARGs)of Malayan pangolin(Manis javanica)intestinal bacteria is ***,multidrug-resistant Escherichia coli M172-1(ST354)isolated from Malayan pangolin feces in 2019 was found to be resistant to 13 *** analysis revealed 4 plas-mids,namely,pM172-1.1,pM172-1.2,pM172-1.3,and pM172-1.4,in the *** pM172-1.2,pM172-1.3,and pM172-1.4 plasmids carried ARGs,namely,IncHI2-HI2A,IncX1-X1,and IncX1,***172-1.3 and pM172-1.4 contained intact IntI1 integrons(Is26/IntI1/arr2/cmlA5/blaOXA-10/ant(3′′)-IIA/dfrA14/Is26).No-tably,pM172-1.3 resulted from the fusion of 2 pM172-1.4 copies and carried many more *** addition to pM172-1.3 from the same host,other drug-resistant bacteria(*** M159-1(ST48),*** S171-1(ST206),and Klebsiella pneumoniae S174-1(ST2354))in the same Malayan pangolin fecal samples also carried 3 plasmids with 100%gene coverage of pM172-1.4 and 99.98%***,ARGs in IncX1 might spread in the intestinalflora of Malayan pangolin and between species via the illegal food chain,posing a potential threat to public health and safety.