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Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice

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World Journal of Gastroenterology 2000年第2期6卷 210-215页

作者： Jin Hui Yuan ru Ping zhang ru gang zhang Li Xia Guo Xing Wang Wang Dan Luo Yong Xie Hong Xie Shanghai Institute of Cell Biology Chinese Academy of SciencesShanghai 200031China Hong Kong University of Science and Technology Hong KongChina

AIM To investigate the effects of taxol onSMMC-7721 human hepatoma and *** In vitro cell growth was assessedby trypan blue exclusion *** model was established by seedingSMMC-7721 cells subcutaneously into Balb/c(nu/nu)nude *** vivo tumor growth wasdetermined by measurement of tumor diameterwith Vernier *** syntheses of DNA,RNA and protein were analyzed by incorporationof ~3H-thymidine,~3H-uridine and ~*** light and electronmicroscopes to observe the morphologicalchanges of cells including mitosis *** Taxol was effective against SMMC-7721 human hepatoma cell growth in the rangesof 2.5 nmol/L-10 nmol/L with mitotic arrestand apoptosis in ***,RNA and proteinsyntheses in cells were also obviouslysuppressed by in vitro treatment of taxol for72 *** at 2.5 nmol/L reduced ~3H-thymidineuptake to about 34% of the control value(P<0.05).Increasing the dose of taxol to20 nmol/L resulted in a greater decrease in ~3H-thymidine incorporation to 60% of the controlvalue(P<0.01).At a concentration of 20 nmol/L,the ~3H-uridine and ~3H-leucine uptakeswere reduced to 52%(P<0.05)and 63%(P<0.01),*** vivo,taxolsignificantly inhibited SMMC-7721 tumor growthat 10 mg/kg,i.p.,once daily for 10 d.A morethan 90% decrease in tumor volume wasobserved by day 11(P<0.01)similarly withmitotic arrest and cell *** Taxol has a marked anticanceractivity in SMMC-7721 human hepatoma both invitro and in nude *** mechanisms might beassociated with mitotic arrest,subsequently,apoptosis of the hepatoma *** obvioustoxicity was observed with in vivoadministration of taxoi.

关键词： paclitaxel liver neoplasms apoptosis mitoics in vitro DNA RNA microscopy wection mice nude

Effects of cisplatin on telomerase activity and telomere length in BEL-7404 human hepatoma cells

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Cell Research 2002年第1期12卷 55-62页

作者： ru gang zhang, ru PING zhang, XING WANG WANG, HONG XIE Department of Biotherapy, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai China

Telomerase activity was inhibited in a dose and time-dependent manner with the treatment of cisplatin for 24, 48, or 72 h in a concentration ranged from 0.8 to 50 1uM in BEL-7404 human hepatoma cells. There were no changes in expression pattern of three telomerase subunits, its catalytic reverse transcriptase subunit (hTERT), its RNA component (hTR) or the associated protein subunit (TP1), after cisplatin treated for 72 h with indicated concentrations. Mean telomere lengths were decreased by the cisplatin treatment. Cell growth inhibition and cell cycle accumulation in G2/M phase were found to be correlated with telomerase inhibition in the present study, but percentages of cell apoptosis did not change markedly during the process.

关键词： Telomerase telomere cisplatin hepatocellular carcinoma cell cycle cell growth.

Telomerase inhibition and telomere loss in BEL-7404 human hepatoma cells treated with doxorubicin

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World Journal of Gastroenterology 2002年第5期8卷 827-831页

作者： ru-gang zhang Li-Xia Guo Xing-Wang Wang Hong Xie Department of Biotherapy,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,the Chinese Academy of Sciences,Shanghai 200031,China Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China.

AIM：To study the effects of doxorub icin on telomerase activity and telomere length in hepatocellular ***:Telomerase activity was assayed with a non-radioisotopic quantitative telomerase repeat amplification protocal-based *** effect of doxorubicin(DOX) on the growth of BEL-7404 human hepatoma cells was the growth of BEL-7404human hepatoma cells was determined by microculture tetrazoloum *** telomere length(terminal restriction fragment)was detected by Southern blot *** expression of telomerase subunits genes was investigated by RT-PCR,Cell apoptosis and cell cycle distribution were evaluated by flow ***:Telomerase activity was inhibited in a dose and time-depandent manner in BEL-7404human hepatoma cells treated with DOXfor24,48or72h in concentrations from 0.156to 2.5μMwhich was crrelated with the inhibition of cell *** changes were found in the mRNA expression of three telomerase subunits(hTERT,hTR and TP1)after drug exposure for 72h with indicated *** cells treated with DOX showed shortened mean telomer length and accumulated at he G2/M ***,there was almost no effects on cell apoptosis by ***:The telomerase inhibition an d the telomere shortening by ODXmay contribute to its efficiency in the treatment in hepatocellular carcinoma.

关键词：阿霉素肝细胞癌端粒酶化学疗法端粒丢失

Text-Image Feature Fine-Grained Learning for Joint Multimodal Aspect-Based Sentiment Analysis

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Computers, Materials & Continua 2025年第1期82卷 279-305页

作者： Tianzhi zhang gang Zhou Shuang zhang Shunhang Li Yepeng Sun Qiankun Pi Shuo Liu School of Data and Target Engineering Information Engineering UniversityZhengzhou450001China Information Engineering Department Liaoning Provincial College of CommunicationsShenyang110122China School of Computer and Artificial Intelligence Zhengzhou UniversityZhengzhou450000China

Joint Multimodal Aspect-based Sentiment Analysis(JMASA)is a significant task in the research of multimodal fine-grained sentiment analysis,which combines two subtasks:Multimodal Aspect Term Extraction(MATE)and Multimodal Aspect-oriented Sentiment Classification(MASC).Currently,most existing models for JMASA only perform text and image feature encoding from a basic level,but often neglect the in-depth analysis of unimodal intrinsic features,which may lead to the low accuracy of aspect term extraction and the poor ability of sentiment prediction due to the insufficient learning of intra-modal *** this problem,we propose a Text-Image Feature Fine-grained Learning(TIFFL)model for ***,we construct an enhanced adjacency matrix of word dependencies and adopt graph convolutional network to learn the syntactic structure features for text,which addresses the context interference problem of identifying different aspect ***,the adjective-noun pairs extracted from image are introduced to enable the semantic representation of visual features more intuitive,which addresses the ambiguous semantic extraction problem during image feature ***,the model performance of aspect term extraction and sentiment polarity prediction can be further optimized and *** on two Twitter benchmark datasets demonstrate that TIFFL achieves competitive results for JMASA,MATE and MASC,thus validating the effectiveness of our proposed methods.

关键词： Multimodal sentiment analysis aspect-based sentiment analysis feature fine-grained learning graph convolutional network adjective-noun pairs

Small extracellular vesicles derived from cerebral endothelial cells with elevated microRNA 27a promote ischemic stroke recovery

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Neural Regeneration Research 2025年第1期20卷 224-233页

作者： Yi zhang Zhongwu Liu Michael Chopp Michael Millman Yanfeng Li Pasquale Cepparulo Amy Kemper Chao Li Li zhang Zheng gang zhang Department of Neurology Henry Ford HospitalDetroitMIUSA Department of Physics Oakland UniversityRochesterMIUSA Department of Pathology Henry Ford HospitalDetroitMIUSA Division of Pharmacology Department of NeuroscienceReproductive and Dentistry SciencesSchool of MedicineUniversity of Naples“Federico Ⅱ”NaplesItaly

Axonal remodeling is a critical aspect of ischemic brain repair processes and contributes to spontaneous functional *** previous in vitro study demonstrated that exosomes/small extracellular vesicles(sEVs)isolated from cerebral endothelial cells(CEC-sEVs)of ischemic brain promote axonal growth of embryonic cortical neurons and that microRNA 27a(miR-27a)is an elevated miRNA in ischemic *** the present study,we investigated whether normal CEC-sEVs engineered to enrich their levels of miR-27a(27a-sEVs)further enhance axonal growth and improve neurological outcomes after ischemic stroke when compared with treatment with non-engineered CEC-sEVs.27a-sEVs were isolated from the conditioned medium of healthy mouse CECs transfected with a lentiviral miR-27a expression *** EVs isolated from CECs transfected with a scramble vector(Scra-sEVs)were used as a *** male mice were subjected to permanent middle cerebral artery occlusion and then were randomly treated with 27a-sEVs or *** array of behavior assays was used to measure neurological *** with treatment of ischemic stroke with Scra-sEVs,treatment with 27a-sEVs significantly augmented axons and spines in the peri-infarct zone and in the corticospinal tract of the spinal grey matter of the denervated side,and significantly improved neurological *** vitro studies demonstrated that CEC-sEVs carrying reduced miR-27a abolished 27a-sEV-augmented axonal *** analysis revealed that 27a-sEVs systemically administered preferentially localized to the pre-synaptic active zone,while quantitative reverse transcription-polymerase chain reaction and Western Blot analysis showed elevated miR-27a,and reduced axonal inhibitory proteins Semaphorin 6A and Ras Homolog Family Member A in the peri-infarct *** of the Clathrin-dependent endocytosis pathway substantially reduced neuronal internalization of *** data provide evidence that 27a-sEVs have

关键词： axonal remodeling cerebral endothelial cells exosomes miR-27a mitochondria Semaphorin 6A small extracellular vesicles stroke

Modeling pedestrian-vehicle interactions at unsignalized mid-block crosswalk under automated vehicle mixed traffic flow conditions: an agent-based approach

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Journal of Traffic and Transportation Engineering(English Edition) 2025年

作者： Haojie Li Binyue Zhao Ziqian zhang Yingheng zhang gang Ren School of Transportation， Southeast University Jiangsu Key Laboratory of Urban ITS Jiangsu Province Collaborative Innovation Center of Modern Urban Traffic Technologies

The introduction of automated vehicles (AVs) is expected to improve the safety and traffic efficiency of the transportation system. However， the impacts of AVs on pedestrian-vehicle interactions (PVIs) remain unclear， especially under the mixed traffic flow condition with AVs and conventional vehicles (CVs). The objective of this paper is to investigate PVIs under AV mixed traffic flow at unsignalized mid-block crosswalk (UMC) where the right of way competition exists and the traffic conditions are more complex. An agent-based simulation model is proposed to evaluate the safety and traffic efficiency under a diverse range of different scenarios. Some agents’ behavioral parameters are calibrated from trajectory and video data collected at two sites in Nanjing. The proposed model is also validated by comparing Post-Encroachment Time (PET) and pedestrian waiting time outputted from simulation with those extracted from empirical data. The simulation results suggest that the introduction of AVs has limited effect on both safety and efficiency levels at UMC under the low AV penetration rate. Compared to the negotiated yielding strategy of AVs， the conservative yielding strategy performs better in terms of pedestrian safety， but worse in improving traffic efficiency. Moreover， the results reveal that pedestrian and vehicle volumes， the proportion of pedestrians familiar with AVs， and the proportion of risk-prone pedestrians could significantly affect the safety and efficiency levels at UMC. Lastly， we provide some practical recommendations for determining treatments for mid-block crosswalks with AV mixed traffic flow to improve pedestrian safety and traffic efficiency.

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Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

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Neural Regeneration Research 2025年第2期20卷 557-573页

作者： Jiewen Chen Xiaolin Zeng Le Wang Wenwu zhang gang Li Xing Cheng Peiqiang Su Yong Wan Xiang Li Department of Spine Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Province Key Laboratory of Orthopedics and Traumatology GuangzhouGuangdong ProvinceChina

Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord *** and astrocytes play key roles in the spinal cord injury micro-environment and share a close ***,the mechanisms involved remain *** this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming *** expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord *** vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between *** further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling ***,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling *** vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function ***,Gas6 may play a role in the treatment of spinal cord *** can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.

关键词： astrocytes Axl cell polarization Gas6 Hippo signal inflammatory micro-environment intercellular interaction microglia single-cell sequencing spinal cord injury

急性胰腺炎患者血清指标PCT、TNF-α及IL-8动态监测的临床应用价值

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中国实用医刊 2018年第5期45卷 48-50页

作者：张茹秦刚张书芬长治医学院附属和济医院检验科山西长治046000

目的分析对急性胰腺炎疾病患者进行血清降钙素原（PCT）、肿瘤坏死因子-α（TNF-α）及白细胞介素-8（IL-8）动态监测的临床应用价值。方法选取急性胰腺炎患者60例，根据患者病情程度将23例轻症急性胰腺炎患者设为观察组1，37例重症急... 详细信息

目的分析对急性胰腺炎疾病患者进行血清降钙素原（PCT）、肿瘤坏死因子-α（TNF-α）及白细胞介素-8（IL-8）动态监测的临床应用价值。方法选取急性胰腺炎患者60例，根据患者病情程度将23例轻症急性胰腺炎患者设为观察组1，37例重症急性胰腺炎患者设为观察组2，将观察组2患者中30例合并感染者设为观察亚组3，7例无合并感染者设为观察亚组4。于入院第1、3、7天检测所有患者血清PCT、TNF-α、IL-8水平，归纳分析上述指标动态监测对急性胰腺炎的临床诊断价值。结果观察组2不同时间段PCT、TNF-α和LI-8指标均高于观察组1，差异有统计学意义（P〈0.05）；观察组2中观察亚组3不同时间段血清PCT、TNF-α和LI-8指标均高于观察亚组4，但仅两亚组PCT水平比较差异有统计学意义（P〈0.05）。结论TNF-α和IL-8有助于判定急性胰腺炎患者病情发展程度，PCT则对于鉴别是否合并感染有突出价值，三种指标动态监测具有简便、准确性高等优势，值得临床推广。

关键词：血清降钙素原肿瘤坏死因子-α 白细胞介素-8

Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice

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World Journal of Gastroenterology 2001年第3期7卷 345-351页

作者： Xing Wang Wang~1 Jin Hui Yuan~1 ru gang zhang~1 Li Xia Guo~1 Yong Xie~2 Hong Xie~1 ~1Department of Biotherapy,Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China ~2Department of Biology,Hong Kong University of Science and Technology,ChinaDr.Xing Wang Wang earned Ph.D.from Shanghai Institute of Materia Medical,Chinese Academy of Sciences in 1997.Now a professor at Shanghai Institute of Cell Biology,Chinese Academy of Sciences. Department of Biotherapy Shanghai Institute of Cell Biology Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 200031 China.

AIM: To evaluate antihepatoma effect of antisense phosphorothioate oligodeoxyribonucleotides (S-ODNs) targeted to alpha-fetoprotein (AFP) genes in vitro and in nude mice. METHODS: AFP gene expression was examined by immunocytochemical method or enzyme-linked immunosorbent assay. Effect of S-ODNs on SMMC-7721 human hepatoma cell growth in vitro was determined using microculture tetrazolium assay. In vitro antitumor activities of S-ODNs were monitored by measuring tumor weight differences in treated and control mice bearing SMMC-7721 xenografts. Induction of cell apoptosis was evaluated by fluorescence-activated cell sorter (FACS) analysis. RESULTS: Antisense S-ODN treatment led to reduced AFP gene expression. Specific antisense S-ODNs, but not control S-ODNs, inhibited the growth of hepatoma cells in vitro. In vitro, only antisense S-ODNs exhibited obvious antitumor activities. FACS analysis revealed that the growth inhibition by antisense S-ODNs was associated with their cell apoptosis induction. CONCLUSION: Antisense S-ODNs targeted to AFP genes inhibit the growth of human hepatoma cells and solid hepatoma, which is related to their cell apoptosis induction.

关键词： Animals Apoptosis Carcinoma, Hepatocellular Gene Expression Gene Therapy Humans In Vitro Liver Neoplasms Male Mice Mice, Inbred BALB C Mice, Nude Neoplasm Transplantation Oligodeoxyribonucleotides, Antisense Research Support, Non-U.S. Gov't Transplantation, Heterologous Tumor Cells, Cultured alpha-Fetoproteins