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Diversity among Isolates of the Tangerine Pathotype of Alternaria alternata

American Journal of Plant sciences 2018年第2期9卷 196-215页

作者： Andréa Celina Ferreira Demartelaere Luciana Cordeiro do Nascimento Cristina O.Marinho Marciano Costa Nunes Patrícia Clemente Abraao rommel s.s.gomes Monica Danielly de Mello Oliveira Larissa Almeida Mirelly M.Porcino Caroline Vargas e silva Wilza C.O.souza Programa de Pós-Graduacao em Agronomia Departamento de Fitotecnia e Ciencias AmbientaisUniversidade Federal da ParaíbaAreiaBrazil

The tangerine pathotype of Alternaria alternata is the aetiological agent of Alternaria brown spot on tangerines. In the state of Paraíba, Brazil, its occurrence on “Dancy” tangerine trees is associated with genetic aspects as well as the influence of environmental conditions on reproduction and dissemination within and between populations. The aim of the present study was to evaluate the diversity of isolates of this pathogen using morphophysiological and molecular markers. For the analysis of mycelial growth and sporulation, 30 isolates from different locations were examined at 24-hour intervals until the seventh day, when the spores were quantified. The 30 isolates were characterised based on molecular markers (IssR) and genetic similarity (Jaccard index). A factor arrangement was used: 30 isolates, four media (ODA, PDA, LEA and V8), three light regimes (continuous dark, alternating light and continuous light) and three temperatures (15°C, 25°C and 35°C), with 12 repetitions. Groups 1, 2 and 3 presented low genetic variability. Group 4 showed high genetic variability of the isolates obtained from the Massaranduba (state of Paraíba-Brazil) producing region and higher mycelial growth and sporulation of A. alternata. The continuous light regime and the temperature 25°C in PDA and V8 media were the ideal conditions for the mycelial growth and sporulation, respectively, of the isolates of A. alternata.

关键词： Citrus tangerine Alternaria Brown spot Fungal Variability Environment Factors IssR Markers

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Advances in gastric cancer research will light the way to control this cancer

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Chinese Journal of Cancer Research 2022年第6期34卷 575-578页

作者： Paulo Pimentel de Assumpcao rommel Rodrigues Burbano Universidade Federal do Para Nucleo de Pesquisas em OncologiaBelem-Para-Brasil 66075-110Brazil Hospital Ophir Loyola Laboratorio de Biologia MoleculaBelém-Para-Brasil 66063-240Brazil

Gastric adenocarcinoma(GC)is one of the most heterogeneous cancers,posing challenges to wide applications of the discoveries when compared to other ***,the benefits of research in the fight against GC are extraordinary,and even taking in mind the immense complexity of this disease,optimism is a great message to take *** advances in GC research will pave the way for GC effective control,helping to save lives,together with permitting sustainable real-life support for those needing complex and high-expense interventions.

关键词： Gastric cancer research innovations

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Evaluation of the viability of nanoparticles in drilling fluids as additive for fluid loss and wellbore stability

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Petroleum 2023年第3期9卷 342-351页

作者： Albertus Retnanto rommel Yrac Abdullah shaat Adhika Retnanto Laith Abughaush Maha Al sulaiti Najla Badar Texas A&M University at Qatar Qatar Georgia Institute of Technology USA

Wellbore instability is an issue that,if left untreated,can cause wells to collapse,resulting in human,environmental,equipment,and revenue *** fluids have been used to enhance the drilling process by lubricating and cooling the drill bit,eliminating cuttings,and most importantly,by improving the stability of the well by preventing fluid ***,there has been an increase in operational demands and challenges that call for drilling fluids to be more effective,economical,sustainable,and environmentally *** shales that have infinitesimally small pores,nanoparticle additives in drilling fluids can be crucial in providing the properties that are necessary to prevent fluid loss and provide wellbore stability while meeting the operational demands of the present ***,this paper examines the use of nanoparticle additives including copper(Ⅱ)oxide(CuO),magnesium oxide(MgO),and aluminum oxide(Al_(2)O_(3))where they are tested under three conditions using the permeable plugging tester(PPT),high-temperature high-pressure(HTHP)fluid loss apparatus,and API low-temperature e low-pressure(LTLP)fluid loss apparatus under concentrations of 0.03%and 0.10%.Finally,based on the results,each nanoparticle sample(particle sizes between one and 100 nm)performed well in contributing to the aim of this *** is the most effective inhibitor across all concentrations and under the three different *** contributed to reducing the fluid loss from 37.6 mL to 18.2 and 13.2 mL,which is between 52%and 65%of fluid *** MgO,it contributed to fluid loss reduction to 23.8 mL and 15 mL,which translated to 37%e60%of fluid loss *** use of Al_(2)O_(3) nanoparticles resulted in a fluid loss reduction to 33.6 mL and 17.8 mL,reducing the fluid loss up to 11%,at HTHP and up to 53%at *** CuO and MgO,Al_(2)O_(3) was less effective under HTHP conditions when compared to LTLP ***_(2)O_(3) did not suffer as a significant diminishing

关键词： Nanoparticles Drilling fluids Additives Fluid loss High-temperature and high-pressure

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shifting Gears

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Beijing Review 2023年第2期66卷 18-19页

作者： rommel C.Banlaoi the Philippine society for Intelligence and security studies

Philippine President Ferdinand Romualdez Marcos Jr. visited China on January 3-5 with the aim of shifting ChinaPhilippines relations to a higher gear. While meeting with Marcos in Beijing, Chinese President Xi Jinping said China views the Philippines as a priority in its neighborhood diplomacy and approaches its relations with the Philippines from a strategic and overall perspective. He added that he is ready “to stay in regular strategic communication with President Marcos and make comprehensive plans for the growth of bilateral ties so that the two countries will stay good neighbors helping each other, good relatives sharing affinity.”

关键词： neighborhood shifting strategic

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Foreword to the special issue on wide-bandgap(WBG)semiconductors:from fundamentals to applications

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Nanotechnology and Precision Engineering 2020年第4期3卷 187-188页

作者： Zongwei Xu Yidan Tang Mathias rommel Tianjin University China Institute of Microelectronics of Chinese Academy of sciences China Fraunhofer Institute for Integrated systems and Device Technology(IIsB) Germany

The development of new kinds of semiconductor material is a very attractive topic of scientific research and *** is considered as one of the most impressive 2D materials for many applications,like graphene-reinforced metal matrix nanocomposites.1 Widebandgap(WBG)semiconductors have received widespread attention in recent years because of their superior physical properties such as large band gap,high carrier mobility,and high thermal *** by silicon carbide(siC)and gallium nitride(GaN),WBG semiconductor materials,therefore,can be operated in extreme working environments or conditions such as high temperature,high frequency,and high power.

关键词： carbide attractive extreme

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Tale of two kinases:Protein kinase A and Ca^(2+)/calmodulin-dependent protein kinase Ⅱ in pre-diabetic cardiomyopathy

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World Journal of Diabetes 2021年第10期12卷 1704-1718页

作者： Pamela Gaitán-González rommel sánchez-Hernández José-Antonio Arias-Montaño Angélica Rueda Department of Biochemistry Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalCiudad de México 07360Mexico Department of Physiology Biophysics and NeurosciencesCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalCiudad de México 07360Mexico

Metabolic syndrome is a pre-diabetic state characterized by several biochemical and physiological alterations,including insulin resistance,visceral fat accumulation,and dyslipidemias,which increase the risk for developing cardiovascular *** syndrome is associated with augmented sympathetic tone,which could account for the etiology of pre-diabetic *** review summarizes the current knowledge of the pathophysiological consequences of enhanced and sustainedβ-adrenergic response in pre-diabetes,focusing on cardiac dysfunction reported in diet-induced experimental models of pre-diabetic *** research reviewed indicates that both protein kinase A and Ca^(2+)/calmodulin-dependent protein kinase Ⅱ play important roles in functional responses mediated byβ1-adrenoceptors;therefore,alterations in the expression or function of these kinases can be *** review also outlines recent information on the role of protein kinase A and Ca^(2+)/calmodulin-dependent protein kinase Ⅱ in abnormal Ca^(2+)handling by cardiomyocytes from diet-induced models of pre-diabetic cardiomyopathy.

关键词： Ca^(2+)/calmodulin-dependent protein kinase II Protein kinase A Metabolic syndrome Pre-diabetes Pre-diabetic cardiomyopathy β-Adrenoceptors

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Clinical implication of 14-3-3 epsilon expression in gastric cancer

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World Journal of Gastroenterology 2012年第13期18卷 1531-1537页

作者： Mariana Ferreira Leal Danielle Queiroz Calcagno smia Demachki Paulo Pimentel Assumpo Roger Chammas rommel Rodríguez Burbano Marília de Arruda Cardoso smith Genetics Division Department of Morphology and GeneticFederal University of So PauloSo Paulo 04023-900Brazil Pathology service Joo de Barros Barreto University HospitalFederal University of ParáBelém 60673-000Brazil surgery service Joo de Barros Barreto University HospitalFederal University of ParáBelém 60673-000Brazil Laboratory of Experimental Oncology School of MedicineUniversity of So PauloSo Paulo 01246-903Brazil Human Cytogenetics Laboratory Institute of Biological SciencesFederal University of ParáBelém 66073-000Brazil

AIM:To evaluate for the first time the protein and mRNA expression of 14-3-3εin gastric ***:14-3-3εprotein expression was determined by western blotting,and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue ***:Authors observed a significant reduction of 14-3-3εprotein expression in gastric cancer(GC)samples compared to their matched non-neoplastic *** levels of 14-3-3εwere also associated with diffuse-type GC and early-onset of this *** data suggest that reduced 14-3-3εmay have a role in gastric carcinogenesis ***:Our results reveal that the reduced 14-3-3εexpression in GC and investigation of 14-3-3ε interaction partners may help to elucidate the carcino-genesis process.

关键词：胃癌临床意义 14-3-3蛋白 RT-PCR检测 mRNA表达量表肿瘤组织气相色谱仪

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Effects of a novel bacterial phytase expressed in Aspergillus Oryzae on digestibility of calcium and phosphorus in diets fed to weanling or growing pigs

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Journal of Animal science and Biotechnology 2013年第2期4卷 102-111页

作者： Ferdinando Nielsen Almeida rommel Casilda sulabo Hans Henrik stein Department of Animal sciences University of Illinois

In 2 experiments, 48 weanling （initial BW： 13.5 ± 2.4 kg, Exp. 1） were used to determine effects of a novel bacterial 6-phytase and 24 growing pigs （initial BW： 36.2 ± 4.0 kg, Exp. 2） expressed in Aspergillus oryzae on the apparent total tract digestibility （ATTD） of phosphorus and calcium in corn-soybean meal diets fed to weanling and growing pigs. In Exp. 1 and 2, pigs were randomly allotted to 6 dietary treatments using a randomized complete block design and a balanced 2 period changeover design, respectively. In both experiments, 6 diets were formulated. The positive control diet was a corn-soybean meal diet with added inorganic phosphorus （Exp. 1：0.42 and 0.86% standardized total tract digestible phosphorus and total calcium, respectively; Exp. 2：0.32 and 0.79% standardized total tract digestible phosphorus and total calcium, respectively）. A negative control diet and 4 diets with the novel phytase （Ronozyme HiPhos, DsM Nutritional Products Inc., Parsippany, N J） added to the negative control diet at levels of 500, 1,000, 2,000, and 4,000 phytase units （FYT）/kg were also formulated. In Exp. 1, the ATTD of phosphorus was greater （P 〈 0.01） for the positive control diet （60.5%） than for the negative control diet （40.5%）, but increased （linear and quadratic, P 〈 0.01） as phytase was added to the negative control diet （40.5% vs. 61.6%, 65.1%, 68.7%, and 68.0%）. The breakpoint for the ATTD of phosphorus （68.4%） was reached at a phytase inclusion level of 1,016 FYT/kg. In Exp. 2, the ATTD of phosphorus was greater （P〈 0.01） for the positive control diet （59.4%） than for the negative control diet （39.8%） and increased （linear and quadratic, P〈 0.01） as phytase was added to the negative control diet （39.8% vs. 58.1%, 65.4%, 69.1%, and 72.8%）. The breakpoint for the ATTD of phosphorus （69.1%） was reached at a phytase inclusion level of 801 FYT/kg. in conclusion, the novel bacterial 6-phytase improved the ATTD of phosphorus and calcium in both weanling and growing pigs.

关键词： Calcium Digestibility Phosphorus Phytase Pigs

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4^(th) generation synchrotron source boosts crystalline imaging at the nanoscale

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Light(science & Applications) 2022年第4期11卷 662-673页

作者： Peng Li Marc Allain Tilman A.Grunewald Marcus rommel Andrea Campos Dina Carbone Virginie Chamard Aix-Marseille Univ CNRSCentrale MarseilleInstitut FresnelMarseilleFrance Nanofabrication Laboratory Department of Microtechnology and NanoscienceMC2Chalmers University of TechnologySE-41296 GothenburgSweden Aix Marseille Univ CNRSCentrale MarseilleFSCM(FR1739)CP2M13397 MarseilleFrance. MAX IV Laboratory Fotongatan 222594 LundSweden Present address:Diamond Light source Harwell Science and Innovation CampusFermi AveDidcot OX110DEUK

New 4^(th)-generation synchrotron sources,with their increased bilince,promise to greatly improve the performances of coherent X-ray *** perspective is of major interest for crystal microscopy,which aims at revealing the 3D crystalline structure of matter at the nanoscale,an approach strongly limited by the available coherent *** results,based on Bragg ptychography experiments performed at the frst 4-generation synchrotron source,demonstrate the possibility of retrieving a high-quality image of the crystalline sample,with unprecedented ***,the larger available coherent flux produces datasets with enough information to overcome experimental limitations,such as strongly deteriorated scanning *** show this achievement would not be posible with 30-generation sources,a limit that has inhibited the development of this otherwise powerful microscopy method,so ***,the advent of next-generation synchrotron sources not only makes Bragg ptychography suitable for high throughput studies but also strongly relaxes the associated experimental constraints,making it compatible with a wider range of experimental set-ups at the new synchrotrons.

关键词： crystalline coherent source

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Density functional theory calculation of the properties of carbon vacancy defects in silicon carbide

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Nanotechnology and Precision Engineering 2020年第4期3卷 211-217页

作者： XiuhongWang Junlei Zhao Zongwei Xu Flyura Djurabekova Mathias rommel Ying song Fengzhou Fanga state Key Laboratory of Precision Measuring Technology&Instruments Centre of MicroNano Manufacturing TechnologyTianjin UniversityTianjin 300072China Department of Physics and Helsinki Institute of Physics University of HelsinkiP.O.Box 43FI-00014 HelsinkiFinland Department of Electrical and Electronic Engineering Southern University of Science and TechnologyShenzhen 518055China Fraunhofer Institute for Integrated systems and Device Technology IIsB Schottkystrasse 1091058 ErlangenGermany

As a promisingmaterial for quantumtechnology,silicon carbide(siC)has attracted great interest inmaterials *** vacancy is a dominant defect in ***,understanding the properties of this defect is critical to its application,and the atomic and electronic structures of the defects needs to be *** this study,density functional theorywas used to characterize the carbon vacancy defects in hexagonal(h)and cubic(k)lattice *** zero-phonon line energies,hyperfine tensors,and formation energies of carbon vacancies with different charge states(2−,−,0,+and 2+)in different supercells(72,128,400 and 576 atoms)were calculated using standard Perdew-Burke-Ernzerhof and Heyd-scuseria-Ernzerhof *** show that the zero-phonon line energies of carbon vacancy defects are much lower than those of divacancy defects,indicating that the former is more likely to reach the excited state than the *** hyperfine tensors of VC+(h)and VC+(k)were *** of the calculated hyperfine tensor with the experimental results indicates the existence of carbon vacancies in siC *** calculation of formation energy shows that the most stable carbon vacancy defects in the material are VC 2+(k),VC+(k),VC(k),VC−(k)and VC 2−(k)as the electronic chemical potential increases.

关键词： Density functional theory silicon carbide Carbon vacancy

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