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A Vision-based Robotic Navigation Method Using an Evolutionary and Fuzzy Q-Learning Approach

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Journal of Artificial Intelligence and Technology 2024年第4期4卷 363-369页

作者： roberto Cuesta-solano Ernesto Moya-Albor Jorge Brieva Hiram Ponce Facultad de Ingeniería Universidad PanamericanaAugusto Rodin 498Ciudad de México 03920México

The paper presents a fuzzy Q-learning(FQL)and optical flow-based autonomous navigation *** FQL method takes decisions in an unknown environment and without mapping,using motion information and through a reinforcement signal into an evolutionary *** reinforcement signal is calculated by estimating the optical flow densities in areas of the camera to determine whether they are“dense”or“thin”which has a relationship with the proximity of *** results obtained show that the present approach improves the rate of learning compared with a method with a simple reward system and without the evolutionary *** proposed system was implemented in a virtual robotics system using the CoppeliaSim software and in communication with Python.

关键词： CoppeliaSim evolutionary algorithm fuzzy Q-learning optical flow reinforced learning vision-based control navigation

DNA features beyond the transcription factor binding site specify target recognition by plant MYC2-related bHLH proteins

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Plant Communications 2021年第6期2卷 27-43页

作者： Irene Lopez-Vidriero Marta Godoy Joaquin Grau Maria Penuelas roberto solano Jose M.Franco-Zorrilla Genomics Unit Centro Nacional de BiotecnologiaCSICC/Darwin 328049 MadridSpain Department of Plant Molecular Genetics Centro Nacional de BiotecnologiaCSICC/Darwin 328049 MadridSpain

Transcription factors(TFs)regulate gene expression by binding to cis-regulatory sequences in the promoters of target *** research is helping to decipher in part the cis-regulatory code in eukaryotes,including plants,but it is not yet fully understood how paralogous TFs select their *** we addressed this question by studying several proteins of the basic helix-loop-helix(bHLH)family of plant TFs,all of which recognize the same DNA *** focused on the MYC-related group of bHLHs,that redundantly regulate the jasmonate(JA)signaling pathway,and we observed a high correspondence between DNA-binding profiles in vitro and MYC function in *** demonstrated that A/T-rich modules flanking the MYC-binding motif,conserved from bryophytes to higher plants,are essential for TF recogni*** observed particular DNA-shape features associated with A/T modules,indicating that the DNA shape may contribute to MYC DNA *** extended this analysis to 20 additional bHLHs and observed correspondence between in vitro binding and protein function,but it could not be attributed to A/T modules as in *** conclude that different bHLHs may have their own codes for DNA binding and specific selection of targets that,at least in the case of MYCs,depend on the TF-DNA interplay.

关键词： transcription factor bHLH target specificity plants

Regulation of floral senescence in Arabidopsis by coordinated action of CONSTANS and jasmonate signaling

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Molecular Plant 2022年第11期15卷 1710-1724页

作者： Gloria Serrano-Bueno Pedro de los Reyes Andrea Chini Gabriel Ferreras-Garrucho Víctor Sánchez de Medina-Hernández Marta Boter roberto solano Federico Valverde Plant Development Group Institute for Plant Biochemistry and PhotosynthesisCSIC-Universidad de Sevilla41092 SevillaSpain Department of Plant Molecular Genetics Centro Nacional de BiotecnologíaCSIC28049 MadridSpain Crop Science Centre University of CambridgeCambridge CB3 OLE CambridgeUK Gregor Mendel Institute(GMI) Austrian Academy of SciencesVienna BioCenter(VBC)ViennaAustria

In Arabidopsis,photoperiodic flowering is controlled by the regulatory hub gene CONSTANS(CO),whereas floral organ senescence is regulated by the jasmonates(JAs).Because these processes are chronologically ordered,it remains unknown whether there are common regulators of both *** this study,we discovered that CO protein accumulates in Arabidopsis flowers after floral induction,and it displays a diurnal pattern in floral organs different from that in the *** observed that altered CO expression could affect flower senescence and abscission by interfering with JA response,as shown by petal-specific transcriptomic analysis as well as CO overexpression in JA synthesis and signaling *** found that CO has a ZIM(ZINC-FINGER INFLORESCENCE MERISTEM)like domain that mediates its interaction with the JA response repressor JAZ3(jasmonate ZIM-domain 3).Their interaction inhibits the repressor activity of JAZ3,resulting in activation of downstream transcription factors involved in promoting flower ***,we showed that CO,JAZ3,and the E3 ubiquitin ligase COI1(Coronatine Insensitive 1)could form a protein complex in planta,which promotes the degradation of both CO and JAZ3 in the presence of *** together,our results indicate that CO,a key regulator of photoperiodic flowering,is also involved in promoting flower senescence and abscission by augmenting JA signaling and *** propose that coordinated recruitment of photoperiodic and JA signaling pathways could be an efficient way for plants to chronologically order floral processes and ensure the success of offspring production.

关键词： CONSTANS flower senescence jasmonic acid

A Single JAZ Repressor Controls the Jasmonate Pathway in Marchantia polymorpha

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Molecular Plant 2019年第2期12卷 185-198页

作者： Isabel Monte Jose M. Franco-Zorrilla Gloria Garcia-Casado Angel M. Zamarreno Jose M. Garcia-Mina Ryuichi Nishihama Takayuki Kohchi roberto solano Department of Plant Molecular Genetics Centro Nacional de Biotecnologia Consejo Superior de Investigaciones Cientificas (CNB-CSIC) 28049 Madrid Spain Genomics Unit Centro Nacional de Biotecnologia Consejo Superior de Investigaciones Cientificas (CNB-CSIC) 28049 Madrid Spain Environmental Biology Department University of Navarra Navarre Spain Graduate School of Biostudies Kyoto University Kyoto 606-8502 Japan

JAZ proteins are negative regulators of jasm onate responses,acting both as repressors of transcription factors and as co-receptors of JA-lle. The high redundancy of JAZ genes in angiosperms has hindered the characterization of a complete depletion of JAZ function. Moreover, the recent discovery that dn- OPDA is the jasmonate ligand in Marchantia polymorpha demonstrates that JA-lle is not the sole COI1/ JAZ ligand in land plants and highlights the importance of studying JAZ co-receptors in bryophytes? Here, we have exploited the low gene redundancy of the liverwort M. polymorpha to characterize the single MpJ4Z in this early diverging plant lineage. We clarify the phylogenetic history of the TIFY family, demonstrate that MpJAZ is the ortholog of AtJAZ with a conserved function, and characterize its repressor activity of dn-OPDA resp on ses. Our results show that, con sistent with previous findings in Arabidopsis, MpJAZ represses jasmonates biosynthesis, senescence, and plant defenses, and promotes cell growth and reproductive fitness, highlighting the power of studies in Marchantia.

关键词： Jasmonate repressors Marchantia Hormone signaling evolution gene redundancy

The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the geminiviral infection in Arabidopsis

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Journal of Integrative Plant Biology 2023年第7期65卷 1826-1840页

作者： Tabata Rosas-Diaz Pepe Cana-Quijada Mengshi Wu Du Hui Gemma Fernandez-Barbero Alberto PMacho roberto solano Araceli GCastillo Xiao-Wei Wang Rosa Lozano-Duran Eduardo R.Bejarano Departamento de Biología Celular Genética y FisiologíaFacultad de CienciasInstituto de Hortofruticultura Subtropical y Mediterránea“La Mayora”(IHSM‐UMA‐CSIC)Universidad de MálagaMálagaSpain Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Institute of Insect Sciences Zhejiang UniversityHangzhou 310058China Departamento de Genética Molecular de Plantas Centro Nacional de Biotecnología‐Consejo Superior de Investigaciones CientíficasMadrid 28049Spain Department of Plant Biochemistry Centre for Plant Molecular BiologyEberhard Karls UniversityTübingen D‐72076Germany

Jasmonates(JAs) are phytohormones that finely regulate critical biological processes, including plant development and defense. JASMONATE ZIM-DOMAIN(JAZ) proteins are crucial transcriptional regulators that keep JA-responsive genes in a repressed state. In the presence of JA-Ile, JAZ repressors are ubiquitinated and targeted for degradation by the ubiquitin/proteasome system,allowing the activation of downstream transcription factors and, consequently, the induction of JA-responsive genes. A growing body of evidence has shown that JA signaling is crucial in defending against plant viruses and their insect vectors. Here, we describe the interaction of C2proteins from two tomato-infecting geminiviruses from the genus Begomovirus, tomato yellow leaf curl virus(TYLCV) and tomato yellow curl Sardinia virus(TYLCSaV), with the transcriptional repressor JAZ8 from Arabidopsis thaliana and its closest orthologue in tomato, SlJAZ9. Both JAZ and C2proteins colocalize in the nucleus, forming discrete nuclear speckles. Overexpression of JAZ8did not lead to altered responses to TYLCV infection in Arabidopsis;however, knock-down of JAZ8 favors geminiviral infection. Low levels of JAZ8 likely affect the viral infection specifically,since JAZ8-silenced plants neither display obvious developmental phenotypes nor present differences in their interaction with the viral insect vector. In summary, our results show that the geminivirus-encoded C2 interacts with JAZ8 in the nucleus, and suggest that this plant protein exerts an anti-geminiviral effect.

关键词： C2 protein geminivirus jasmonates JAZ8 transcriptional repressor TYLCV

Arabidopsis SWC4 Binds DNA and Recruits the SWR1 Complex to Modulate Histone H2A.Z Deposition at Key Regulatory Genes

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Molecular Plant 2018年第6期11卷 815-832页

作者： Angeles Gomez-Zambrano Pedro Crevillen Jose M. Franco-Zorrilla Juan A. Lopez Jordi Moreno-Romero Pawel Roszak Juan Santos-Gonzalez Silvia Jurado Jesus Vazquez Claudia Kohler roberto solano Manuel Pineiro Jose A. Jarillo Centro de Biotecnologia y Genomica de Plantas Universidad Politecnica de Madrid (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) Campus Montegancedo UPM Pozuelo de Alarcon 28223 Madrid Spain Plant Molecular Genetics Department and Genomics Unit Centro Nacional de Biotecnologia Consejo Superior de Investigaciones Cientfficas 28049 Madrid Spain Proteomics Unit Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) 28029 Madrid Spain Department of Plant Biology Uppsala BioCenter Swedish University of Agricultural Sciences and Linnean Center for Plant Biology Uppsala 75652 Sweden Laboratory of Cardiovascular Proteomics Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) 28029 Madrid Spain Instituto de Bioquimica Vegetal y Fotosintesis Consejo Superior de Investigaciones Cientfficas and Universidad de Sevilla 41092 Sevilla Spain Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB Campus UAB Bellaterra Barcelona Spain The Sainsbury Laboratory Cambridge CB2 1LR UK

Deposition of the H2A.Z histone variant by the SWR1 complex （SWRI-C） in regulatory regions of specific loci modulates transcription. Characterization of mutations in Arabidopsis thaliana homologs of yeast SWRI-C has revealed a role for H2A.Z exchange in a variety of developmental processes. Nevertheless, the exact composition of plant SWRI-C and how it is recruited to target genes remains to be established. Here we show that SWC4, the Arabidopsis homolog of yeast SANT domain protein Swc4/Eaf2, is a DNA-binding protein that interacts with SWR1-C subunits. We demonstrate that the swc4-1 knockout mutant is embryo- lethal, while SWC4 RNAi knockdown lines display pleiotropic phenotypic alterations in vegetative and repro- ductive traits, including acceleration of flowering time, indicating that SWC4 controls post-embryonic processes. Transcriptomic analyses and genome-wide profiling of H2A.Z indicate that SWC4 represses tran- scription of a number of genes, including the floral integrator FT and key transcription factors, mainly by modulating H2A.Z deposition. Interestingly, SWC4 silencing does not affect H2A.Z deposition at the FLC locus nor expression of this gene, a master regulator of flowering previously shown to be controlled by SWR1-C. Importantly, we find that SWC4 recognizes specific AT-rich DNA elements in the chromatin regions of target genes and that SWC4 silencing impairs SWRI-C binding at FT. Collectively, our data suggest that SWC4 regulates plant growth and development by aiding SWR1-C recruitment and modulating H2A.Z deposition.

关键词： Arabidopsis SWC4 chromatin SWR1 complex H2A.Z deposition flowering time

Interleukin 1βreceptor and synaptic dysfunction in recurrent brain infection with Herpes simplex virus type-1

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Neural Regeneration Research 2025年第2期20卷 416-423页

作者： roberto Piacentini Claudio Grassi Department of Neuroscience UniversitàCattolica del Sacro CuoreRomeItaly Fondazione Policlinico Universitario A.Gemelli IRCCSRomeItaly

Several experimental evidence suggests a link between brain Herpes simplex virus type-1 infection and the occurrence of Alzheimer’s ***,the molecular mechanisms underlying this association are not completely *** the molecular mediators of synaptic and cognitive dysfunction occurring after Herpes simplex virus type-1 infection and reactivation in the brain neuroinflammatory cytokines seem to occupy a central ***,we specifically reviewed literature reports dealing with the impact of neuroinflammation on synaptic dysfunction observed after recurrent Herpes simplex virus type-1 reactivation in the brain,highlighting the role of interleukins and,in particular,interleukin 1βas a possible target against Herpes simplex virus type-1-induced neuronal dysfunctions.

关键词： herpes simplex virus type 1 interleukin 1β microglia neuroinflammation synaptic dysfunction

Syndecans in Alzheimer's disease:pathogenetic mechanisms and potential therapeutic targets

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Neural Regeneration Research 2025年第9期20卷 2594-2595页

作者： Carmela Rita Balistreri roberto Monastero Cellular Molecular and Clinical Pathological LaboratoryDepartment of BiomedicineNeuroscience and Advanced Diagnostics(Bi.N.D.)University of PalermoPalermoItaly Memory and Parkinson’s disease Center Policlinico“Paolo Giaccone”PalermoDepartment of BiomedicineNeuroscience and Advanced Diagnostics(Bi.N.D.)University of PalermoVia La Loggia 1PalermoItaly

With increasing age,humans become more susceptible to the onset of neurodegenerative diseases(NDs),and among these,Alzheimer's disease(AD)is the most frequent(Nicoletti et al.,2023).NDs are primarily characterized by neuronal loss and atrophy,but also by lesions involving the cerebral and/or cardiovascular system(Balistreri,2021).Lesions such as macroinfa rcts,microinfarcts,hemorrhages,white matter lesions,atherosclerosis,and arteriolosclerosis have also been significantly described in the preclinical stages of cognitive impairment characterizing NDs(Mariani et al.,2007).

关键词： Alzheimer cardiovascular

Therapeutic targeting of cellular prion protein: toward the development of dual mechanism anti-prion compounds

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Neural Regeneration Research 2025年第4期20卷 1009-1014页

作者： Antonio Masone Chiara Zucchelli Enrico Caruso Giovanna Musco roberto Chiesa Laboratory of Prion Neurobiology Department of NeuroscienceIstituto di Ricerche Farmacologiche Mario Negri IRCCSMilanItaly Biomolecular NMR Unit Division of Genetics and Cell BiologyIRCCS Ospedale San RaffaeleMilanItaly Department of Biotechnology and Life Sciences University of InsubriaVareseItaly

PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other *** adopt different pathogenic conformations(prion strains),which can be resistant to potential drugs,or acquire drug resistance,posing challenges for the development of effective *** PrPCis the obligate precursor of any prion strain and serves as the mediator of prion neurotoxicity,it represents an attractive therapeutic target fo r prion *** this minireview,we briefly outline the approaches to target PrPCand discuss our recent identification of Zn(Ⅱ)-Bn PyP,a PrPC-targeting porphyrin with an unprecedented bimodal mechanism of *** argue that in-depth understanding of the molecular mechanism by which Zn(Ⅱ)-Bn PyP targets PrPCmay lead toward the development of a new class of dual mechanism anti-prion compounds.

关键词： anti-prion drug anti-PrPC antibody antisense oligonucleotide neurodegeneration pharmacological chaperone porphyrin prion disease PrPC degrader PrPC shedding zinc finger repressor