DNA features beyond the transcription factor binding site specify target recognition by plant MYC2-related bHLH proteins

作     者：Irene Lopez-Vidriero Marta Godoy Joaquin Grau Maria Penuelas Roberto Solano Jose M.Franco-Zorrilla 

作者机构：Genomics UnitCentro Nacional de BiotecnologiaCSICC/Darwin 328049 MadridSpain Department of Plant Molecular GeneticsCentro Nacional de BiotecnologiaCSICC/Darwin 328049 MadridSpain 

出 版 物：《Plant Communications》 (植物通讯（英文）)

年 卷 期：2021年第2卷第6期

页      面：27-43页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：Ministerio de Economía y Competitividad, MINECO Ministerio de Ciencia e Innovación, MICINN, (BIO2017-86651-P) European Regional Development Fund, ERDF, (BIO2016-77216-R) 

主　　题：transcription factor bHLH target specificity plants 

摘      要：Transcription factors(TFs)regulate gene expression by binding to cis-regulatory sequences in the promoters of target *** research is helping to decipher in part the cis-regulatory code in eukaryotes,including plants,but it is not yet fully understood how paralogous TFs select their *** we addressed this question by studying several proteins of the basic helix-loop-helix(bHLH)family of plant TFs,all of which recognize the same DNA *** focused on the MYC-related group of bHLHs,that redundantly regulate the jasmonate(JA)signaling pathway,and we observed a high correspondence between DNA-binding profiles in vitro and MYC function in *** demonstrated that A/T-rich modules flanking the MYC-binding motif,conserved from bryophytes to higher plants,are essential for TF recogni*** observed particular DNA-shape features associated with A/T modules,indicating that the DNA shape may contribute to MYC DNA *** extended this analysis to 20 additional bHLHs and observed correspondence between in vitro binding and protein function,but it could not be attributed to A/T modules as in *** conclude that different bHLHs may have their own codes for DNA binding and specific selection of targets that,at least in the case of MYCs,depend on the TF-DNA interplay.