The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the geminiviral infection in Arabidopsis

作     者：Tabata Rosas-Diaz Pepe Cana-Quijada Mengshi Wu Du Hui Gemma Fernandez-Barbero Alberto PMacho Roberto Solano Araceli GCastillo Xiao-Wei Wang Rosa Lozano-Duran Eduardo R.Bejarano Tabata Rosas-Diaz;Pepe Cana-Quijada;Mengshi Wu;Du Hui;Gemma Fernandez-Barbero;Alberto P.Macho;Roberto Solano;Araceli G.Castillo;Xiao-Wei Wang;Rosa Lozano-Duran;Eduardo R.Bejarano

作者机构：Departamento de Biología CelularGenética y FisiologíaFacultad de CienciasInstituto de Hortofruticultura Subtropical y Mediterránea“La Mayora”(IHSM‐UMA‐CSIC)Universidad de MálagaMálagaSpain Shanghai Center for Plant Stress BiologyCAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Institute of Insect SciencesZhejiang UniversityHangzhou 310058China Departamento de Genética Molecular de PlantasCentro Nacional de Biotecnología‐Consejo Superior de Investigaciones CientíficasMadrid 28049Spain Department of Plant BiochemistryCentre for Plant Molecular BiologyEberhard Karls UniversityTübingen D‐72076Germany 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第7期

页      面：1826-1840页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：supported by a President's International Fellowship Initiative (PIFI) postdoctoral fel owship (No. 2016PB042) from the Chinese Academy of Sciences the “Programa Juan de la Cierva” (IJCI-2017-33367) from the MCIN and FEDER program UMA20-FEDERJA-132 by AEI and by “ERDF A way of making Europe,” by the “European Union” Funding for Open Access charge: Universidad de Málaga / CBUA 

主　　题：C2 protein geminivirus jasmonates JAZ8 transcriptional repressor TYLCV 

摘      要：Jasmonates(JAs) are phytohormones that finely regulate critical biological processes, including plant development and defense. JASMONATE ZIM-DOMAIN(JAZ) proteins are crucial transcriptional regulators that keep JA-responsive genes in a repressed state. In the presence of JA-Ile, JAZ repressors are ubiquitinated and targeted for degradation by the ubiquitin/proteasome system,allowing the activation of downstream transcription factors and, consequently, the induction of JA-responsive genes. A growing body of evidence has shown that JA signaling is crucial in defending against plant viruses and their insect vectors. Here, we describe the interaction of C2proteins from two tomato-infecting geminiviruses from the genus Begomovirus, tomato yellow leaf curl virus(TYLCV) and tomato yellow curl Sardinia virus(TYLCSaV), with the transcriptional repressor JAZ8 from Arabidopsis thaliana and its closest orthologue in tomato, SlJAZ9. Both JAZ and C2proteins colocalize in the nucleus, forming discrete nuclear speckles. Overexpression of JAZ8did not lead to altered responses to TYLCV infection in Arabidopsis;however, knock-down of JAZ8 favors geminiviral infection. Low levels of JAZ8 likely affect the viral infection specifically,since JAZ8-silenced plants neither display obvious developmental phenotypes nor present differences in their interaction with the viral insect vector. In summary, our results show that the geminivirus-encoded C2 interacts with JAZ8 in the nucleus, and suggest that this plant protein exerts an anti-geminiviral effect.