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The RNA m^(6)A demethylase ALKBH5 drives emergency granulopoiesis and neutrophil mobilization by upregulating G-CSFR expression

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Cellular & Molecular Immunology 2024年第1期21卷 6-18页

作者： Yang Liu renjie Song Zhike lu lu Zhao Xinyi Zhan Yini Li Xuetao Cao Department of Immunology Center for ImmunotherapyInstitute of Basic Medical SciencesPeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Suzhou Institute of Systems Medicine Chinese Academy of Medical SciencesSuzhouChina Frontier Research Center for Cell Response Institute of ImmunologyCollege of Life SciencesNankai UniversityTianjinChina School of Life Sciences Westlake UniversityHangzhouChina

Emergency granulopoiesis and neutrophil mobilization that can be triggered by granulocyte colony-stimulating factor(G-CSF)through its receptor G-CSFR are essential for antibacterial innate defense.However,the epigenetic modifiers crucial for intrinsically regulating G-CSFR expression and the antibacterial response of neutrophils remain largely unclear.N6-methyladenosine(m^(6)A)RNA modification and the related demethylase alkB homolog 5(ALKBH5)are key epigenetic regulators of immunity and inflammation,but their roles in neutrophil production and mobilization are still unknown.We used cecal ligation and puncture(CLP)-induced polymicrobial sepsis to model systemic bacterial infection,and we report that ALKBH5 is required for emergency granulopoiesis and neutrophil mobilization.ALKBH5 depletion significantly impaired the production of immature neutrophils in the bone marrow of septic mice.In addition,Alkbh5-deficient septic mice exhibited higher retention of mature neutrophils in the bone marrow and defective neutrophil release into the circulation,which led to fewer neutrophils at the infection site than in their wild-type littermates.During bacterial infection,ALKBH5 imprinted production-and mobilization-promoting transcriptome signatures in both mouse and human neutrophils.Mechanistically,ALKBH5 erased m^(6)A methylation on the CSF3R mRNA to increase the mRNA stability and protein expression of G-CSFR,consequently upregulating cell surface G-CSFR expression and downstream STAT3 signaling in neutrophils.The RIP-qPCR results confirmed the direct binding of ALKBH5 to the CSF3R mRNA,and the binding strength declined upon bacterial infection,accounting for the decrease in G-CSFR expression on bacteria-infected neutrophils.Considering these results collectively,we define a new role of ALKBH5 in intrinsically driving neutrophil production and mobilization through m^(6)A demethylation-dependent posttranscriptional regulation,indicating that m^(6)A RNA modification in neutrophils is a potential target for treating bacteria

关键词： Emergency granulopoiesis Neutrophil mobilization ALKBH5 m^(6)A RNA modification G-CSF receptor

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Ionically Imprinting-Based Copper(Ⅱ)Label-Free Detection for Preventing Hearing Loss

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Engineering 2024年第2期33卷 276-282页

作者： Huan Wang Hui Zhang Xiaoli Zhang Hong Chen Ling lu renjie Chai The Eighth Affiliated Hospital Sun Yat-Sen UniversityShenzhen 518033China State Key Laboratory of Digital Medical Engineering Department of Otolaryngology Head and Neck SurgeryZhongda Hospital&Advanced Institute for Life and HealthSoutheast UniversityNanjing 210096China Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging Department of Biomedical EngineeringShenzhen UniversityShenzhen 518060China Department of Otolaryngology Head and Neck Surgery Jiangsu Provincial Key Medical DisciplineNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Institute for Stem Cell and Regeneration Chinese Academy of ScienceBeijing 100101China Beijing Key Laboratory of Neural Regeneration and Repair Capital Medical UniversityBeijing 100069China

Copper is a microelement with important physiological functions in the body.However,the excess copper ion(Cu^(2+))may cause severe health problems,such as hair cell apoptosis and the resultant hearing loss.Therefore,the assay of Cu^(2+)is important.We integrate ionic imprinting technology(IIT)and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads(IIHBs)as a low-cost and high-specificity platform for Cu^(2+)detection.The IIHBs have a macroporous microstructure,uniform size,vivid structural color,and magnetic responsiveness.When incubated in solution,IIHBs recognize Cu^(2+)and exhibit a reflective peak change,thereby achieving label-free detection.In addition,benefiting from the IIT,the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100μmol·L^(-1).These features indicated that the developed IIHBs are promising candidates for Cu^(2+)detection,particularly for the prevention of hearing loss.

关键词： Structural color Microfluidics Ionic imprinting Label-free detection Hearing loss

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Stem Cell-Based Hair Cell Regeneration and Therapy in the Inner Ear

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Neuroscience Bulletin 2024年第1期40卷 113-126页

作者： Jieyu Qi Wenjuan Huang Yicheng lu Xuehan Yang Yinyi Zhou Tian Chen Xiaohan Wang Yafeng Yu Jia-Qiang Sun renjie Chai State Key Laboratory of Digital Medical Engineering Department of Otolaryngology Head and Neck SurgeryZhongda HospitalSchool of Life Sciences and TechnologyAdvanced Institute for Life and HealthJiangsu Province High-Tech Key Laboratory for Bio-Medical ResearchSoutheast UniversityNanjing 210096China Hospital of Southeast University Nanjing 210096China First Affiliated Hospital of Soochow University Suzhou 215006China Department of Otolaryngology-Head and Neck Surgery The First Affliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Institute for Stem Cell and Regeneration Chinese Academy of ScienceBeijing 100101China

Hearing loss has become increasingly prevalent and causes considerable disability,thus gravely burdening the global economy.Irreversible loss of hair cells is a main cause of sensorineural hearing loss,and currently,the only relatively effective clinical treatments are limited to digital hearing equipment like cochlear implants and hearing aids,but these are of limited benefit in patients.It is therefore urgent to understand the mechanisms of damage repair in order to develop new neuroprotective strategies.At present,how to promote the regeneration of functional hair cells is a key scientific question in the field of hearing research.Multi-ple signaling pathways and transcriptional factors trigger the activation of hair cell progenitors and ensure the maturation of newborn hair cells,and in this article,we first review the principal mechanisms underlying hair cell reproduction.We then further discuss therapeutic strategies involving the co-regulation of multiple signaling pathways in order to induce effective functional hair cell regeneration after degeneration,and we summarize current achievements in hair cell regeneration.Lastly,we discuss potential future approaches,such as small molecule drugs and gene therapy,which might be applied for regenerating functional hair cells in the clinic.

关键词： Hearing loss Cochlea-Stem cell Hair cell regeneration

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Cerium Methacrylate Assisted Preparation of Highly Thermally Conductive and Anticorrosive Multifunctional Coatings for Heat Conduction Metals Protection

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纳微快报:英文版 2023年第11期15卷 172-184页

作者： Fei Xu Peng Ye Jianwen Peng Haolei Geng Yexiang Cui Di Bao renjie lu Hongyu Zhu Yanji Zhu Huaiyuan Wang School of Chemical Engineering and Technology Tianjin UniversityTianjin 300350People’s Republic of China Haihe Laboratory of Sustainable Chemical Transformations Tianjin 300192People’s Republic of China Tianjin Key Lab Composite&Functional Materials School of Materials Science and EngineeringTianjin UniversityTianjin 300072People’s Republic of China State Key Laboratory of Chemical Engineering and Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) Tianjin UniversityTianjin 300072People’s Republic of China Tianjin Key Laboratory of Chemical Process Safety and Equipment Technology Tianjin UniversityTianjin 300072People’s Republic of China

Preparing polymeric coatings with well corrosion resistance and high thermal conductivity(TC)to prolong operational life and ensure service reliability of heat conductive metallic materials has long been a substantive and urgent need while a difficult task.Here we report a multifunctional epoxy composite coating(F-CB/CEP)by synthesizing cerium methacrylate and ingeniously using it as a novel curing agent with corrosion inhibit for epoxy resin and modifier for boron nitride through"cation-π"interaction.The prepared F-CB/CEP coating presents a high TC of 4.29 W m^(−1)K^(−1),which is much higher than other reported anti-corrosion polymer coatings and thereby endowing metal materials coated by this coating with outstanding thermal management performance compared with those coated by pure epoxy coating.Meanwhile,the low-frequency impedance remains at 5.1×10^(11)Ωcm^(2)even after 181 days of immersion in 3.5 wt%NaCl solution.Besides,the coating also exhibits well hydrophobicity,self-cleaning properties,temperature resistance and adhesion.This work provides valuable insights for the preparation of high-performance composite coatings with potential to be used as advanced multifunctional thermal management materials,especially for heat conduction metals protection.

关键词： Epoxy coatings Thermal conductivity Anti-corrosion Hydrophobicity Cerium methacrylate

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HTDet:A Clustering Method Using Information Entropy for Hardware Trojan Detection

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Tsinghua Science and Technology 2021年第1期26卷 48-61页

作者： renjie lu Haihua Shen Zhihua Feng Huawei Li Wei Zhao Xiaowei Li the University of Chinese Academy of Sciences Beijing 101408China Beijing Institute of Computer Technology and Application Beijing 100854China the State Key Laboratory of Computer Architecture Institute of Computing TechnologyChinese Academy of SciencesBeijing 100190China

Hardware Trojans(HTs)have drawn increasing attention in both academia and industry because of their significant potential threat.In this paper,we propose HTDet,a novel HT detection method using information entropybased clustering.To maintain high concealment,HTs are usually inserted in the regions with low controllability and low observability,which will result in that Trojan logics have extremely low transitions during the simulation.This implies that the regions with the low transitions will provide much more abundant and more important information for HT detection.The HTDet applies information theory technology and a density-based clustering algorithm called Density-Based Spatial Clustering of Applications with Noise(DBSCAN)to detect all suspicious Trojan logics in the circuit under detection.The DBSCAN is an unsupervised learning algorithm,that can improve the applicability of HTDet.In addition,we develop a heuristic test pattern generation method using mutual information to increase the transitions of suspicious Trojan logics.Experiments on circuit benchmarks demonstrate the effectiveness of HTDet.

关键词： Hardware Trojan(HT)detection information entropy Density-Based Spatial Clustering of Applications with Noise(DBSCAN) unsupervised learning clustering mutual information test patterns generation

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Effect of In doping on the evolution of microstructure,magnetic properties and corrosion resistance of NdFeB magnets

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Chinese Physics B 2024年第3期33卷 623-629页

作者：李豫豪范晓东贾智范璐丁广飞刘新才郭帅郑波曹帅陈仁杰闫阿儒 School of Materials Science and Chemical Engineering Ningbo UniversityNingbo 315211China CISRI & NIMTE Joint Innovation Center for Rare Earth Permanent Magnets Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China University of Chinese Academy of Sciences Beijing 100049China

The grain boundary phase affects the magnetic properties and corrosion resistance of sintered NdFeB magnets.In this work,a small amount of In was added to NdFeB magnets by induction melting to systematically investigate its effect on the evolution of the microstructure,magnetic properties and corrosion resistance of NdFeB magnets.Microstructural analysis illustrated that minor In addition generated more grain boundary phases and an abundant amorphous phase at the triple-junction grain boundary.While the addition of In failed to enhance the magnetic isolation effect between adjacent matrix grains,its incorporation fortuitously elevated the electrochemical potential of the In-containing magnets.Besides,during corrosion,an In-rich precipitate phase formed,hindering the ingress of the corrosive medium into the magnet.Consequently,this significantly bolstered the corrosion resistance of the sintered NdFeB magnets.The phase formation,magnetic properties and corrosion resistance of In-doped NdFeB magnets are detailed in this work,which provides new prospects for the preparation of high-performance sintered NdFeB magnets.

关键词： In-doping NdFeB magnets magnetic properties corrosion resistance

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改进GBS-YOLOv7t的钢材表面缺陷检测

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光电工程 2024年第5期51卷 54-65页

作者：梁礼明龙鹏威卢宝贺李仁杰江西理工大学电气工程与自动化学院江西赣州341000

针对钢材表面缺陷区域小目标居多,现有大部分方法无法均衡检测精度和速度的问题,提出一种基于YOLOv7-tiny的钢材表面缺陷检测算法(GBS-YOLOv7t)。该方法一是设计GAC-FPN网络,采用渐进和跨层的方式充分融合目标语义信息,以改善传统特征... 详细信息

针对钢材表面缺陷区域小目标居多,现有大部分方法无法均衡检测精度和速度的问题,提出一种基于YOLOv7-tiny的钢材表面缺陷检测算法(GBS-YOLOv7t)。该方法一是设计GAC-FPN网络,采用渐进和跨层的方式充分融合目标语义信息,以改善传统特征金字塔中存在限制信息流问题;二是嵌入双层路由注意力模块,使模型具备动态查询和感知稀疏性能力,以提高对小目标的检测精度;三是引入SIoU损失函数,提升模型训练和推理能力,增强网络鲁棒性。最后在公共数据集NEU-DET进行实验验证,mAP和精确度分别为72.9%和69.9%,相较于YOLOv7-tiny原模型分别提升4.2%和8.5%;FPS达到104.1帧,具有较强实时性;与其他检测算法相比,GBS-YOLOv7t算法对钢材表面区域小目标的检测更有效,实验表明改进后的算法能够更好地均衡检测精度和速度。

关键词：缺陷检测 YOLOv7-tiny GAC-FPN网络双层路由注意力 SIoU

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肘关节骨关节炎手术治疗进展

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中华老年骨科与康复电子杂志 2024年第1期10卷 57-64页

作者：陈仁杰鲁谊首都医科大学附属北京积水潭医院运动医学科

肘关节骨关节炎是以关节疼痛、活动受限为主要症状,好发于体力劳动者的退行性病变,随着经济的发展和社会的进步,越来越多的患者受到肘骨关节炎的困扰。其病因尚不明确,可能与肘关节的过度使用有关。肘骨关节炎的治疗包括非手术治疗与手... 详细信息

肘关节骨关节炎是以关节疼痛、活动受限为主要症状,好发于体力劳动者的退行性病变,随着经济的发展和社会的进步,越来越多的患者受到肘骨关节炎的困扰。其病因尚不明确,可能与肘关节的过度使用有关。肘骨关节炎的治疗包括非手术治疗与手术治疗。对于疼痛较轻、关节活动受限不严重的患者,治疗以非手术治疗为主,对经规范非手术治疗无效或症状较为严重的患者,则应考虑手术治疗。目前用以治疗该疾病的手术方式种类较多,最常用手术方式有:切开肘关节成型术、关节镜肘关节成型术、全肘关节置换术。除此之外,还有一些手术方式也被应用于肘关节骨性关节炎的治疗,包括:Outerbridge-Kashiwagi(O-K)手术、肘关节间置成型术、肱桡关节置换术、肱骨远端半肘关节置换术等。各类手术方式对于处理不同类型的肘骨关节炎均有自己独特的优势,如切开肘关节成型术在改善患者肘关节活动度方面更具优势,而关节镜肘关节成型术则有着更低的术后并发症发生率,肘关节间置成型术适用于年轻的肘关节骨关节炎患者,肱桡关节置换术对于发生于肱桡关节的肘骨性关节炎独具优势,因此治疗时选择合适的手术方式尤为重要。本文结合既往文献,就各种治疗肘骨关节炎的手术方式展开综述,探讨各类手术的适应症及优缺点,以期为肘骨关节炎手术治疗时方案选择提供参考。

关键词：肘关节骨关节炎手术治疗关节成型术

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盐酸厄洛替尼与L-色氨酸共无定型物的制备及其大鼠体内药动学研究

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中国医药工业杂志 2024年第2期55卷 244-252页

作者：曹锦许仁杰贺建华肖学成卢山湖北中医药大学药学院湖北中医药大学药物制剂研究中心湖北武汉430065 湖北时珍实验室湖北武汉430065

盐酸厄洛替尼(1)是一种新型酪氨酸激酶抑制剂,渗透性较高但水溶性较低。添加小分子配体制备共无定型物是提高难溶性药物溶解度、溶出速率和生物利用度的有效策略。该研究以L-色氨酸(2)为小分子配体,通过旋转蒸发法制备1-2共无定型物,并... 详细信息

盐酸厄洛替尼(1)是一种新型酪氨酸激酶抑制剂,渗透性较高但水溶性较低。添加小分子配体制备共无定型物是提高难溶性药物溶解度、溶出速率和生物利用度的有效策略。该研究以L-色氨酸(2)为小分子配体,通过旋转蒸发法制备1-2共无定型物,并采用傅里叶变换红外光谱法(FTIR)、粉末X射线衍射法(PXRD)和差示扫描量热法(DSC)等技术进行表征。PXRD和DSC结果证明共无定型物的形成。FTIR结果和分子动力学模拟(MD)进一步说明1与2之间可能存在氢键相互作用,可能是共无定型体系形成的潜在分子机制。与单一1晶体相比,共无定型物中1的溶解度和溶出速率均明显提高。SD大鼠体内药动学模型拟合结果表明,共无定型物组c_(max)和AUC_(0→t)分别为1原料药组的2.8和2.3倍,而物理混合物组c_(max)和AUC_(0→t)并未显著提高。该研究制得的1-2共无定型物可显著提高1的溶解度和口服利用生物度。

关键词：盐酸厄洛替尼 L-色氨酸共无定型体系溶解度药代动力学

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基于CiteSpace近10年蛇咬伤治疗研究进展的知识图谱可视化分析

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黑龙江科学 2024年第6期15卷 100-103页

作者：鲁江龙韦仁杰韦奇右江民族医学院研究生学院广西百色533000 右江民族医学院附属河池市人民医院创伤骨科广西河池547000

为探讨蛇咬伤治疗研究的发展动态及趋势,检索中国知网2014年1月1日—2023年7月30日蛇咬伤治疗相关文献,使用CiteSpace软件对相关文献进行知识图谱绘制,从发文量、作者、机构、关键词等方面进行可视化分析。结果表明,在纳入分析的503篇... 详细信息

为探讨蛇咬伤治疗研究的发展动态及趋势,检索中国知网2014年1月1日—2023年7月30日蛇咬伤治疗相关文献,使用CiteSpace软件对相关文献进行知识图谱绘制,从发文量、作者、机构、关键词等方面进行可视化分析。结果表明,在纳入分析的503篇文献中,近10年整体发文量呈波动式下降,发文量最多的作者是王万春、龚旭初、罗毅等,发文量较多的机构是江西中医药大学附属医院、广州中医药大学附属新会中医院、安徽中医药大学第一附属医院等,虽然医药大学及所属医院内部联系较密切,但院校之间合作不足。关键词中心性排名依次为临床疗效、预后、毒蛇咬伤、严重程度等,研究热点主要集中在机械通气、血液净化、凝血功能、炎症反应。说明蛇咬伤治疗研究日趋完善,但相应机构之间的合作不紧密,治疗存在地域差异,相关热点研究较少。

关键词：蛇咬伤 CiteSpace 知识图谱可视化分析

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