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Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

Journal of Animal Science and Biotechnology 2017年第2期8卷 370-377页

作者： J.E.Hernández-Pichardo Y.Ducolomb S.Romo M.E.Kjelland R.Fierro F.Casillas M.betancourt División de Ciencias Biológicas y de la Salud Departamento de Producción Agrícola y AnimalUniversidad Autónoma Metropolitana-XochimilcoCiudad de MexicoMexico Division de Ciencias Biologicas y de la Salud Departamento de Ciencias de la SaludUniversidad Autónoma Metropolitana-IztapalapaSan Rafael Atlixco 186CP 09340 Ciudad de MexicoMexico Departamento de Ciencias Pecuarias Facultad de EstudiosSuperiores CuautitlanUNAMCiudad de MexicoEstado de MexicoMexico Conservation Genetics&BiotechLLCValley CityNDUSA Division de Ciencias Biologicas y de la Salud Departamento de Biologia de la ReproduccionUniversidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico Doctorado en Biologia Experimental Universidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico

Background： In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically activated ovine oocytes and sperm selected by swim up（SU） or swim up ＋ zona pellucida（SU ＋ ZP） ***： Experiment 1, 4–20 replicates with total 821 in vitro matured oocytes were chemically activated with ethanol, calcium ionophore or ionomycin, to determine oocyte activation（precense of one PN）. Treatments showed similar results（54, 47, 42 %, respectively） but statistically differents（P 0.05）.Conclusions： Chemical activation induces higher ovine oocyte activation than mechanical activation. Ethanol slightly displays higher oocyte activation than calcium ionophore and ionomicine. Sperm selection with SU ＋ ZP increased AR/A and AR/D rates in comparison with SU in fresh and frozen-thawed sperm. According to this, in terms of fertilization rates, chemical activation after ICSI increased oocyte PN formation compared to mechanical activation. Also, fresh sperm treated with SU and SU ＋ ZP were significantly different than frozen-thawed sperm,but between sperm treatments no significant differences were obtained.

关键词： Ethanol ICSI Oocyte activation Ovine Pronucleus Zona pellucida

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益生菌通过调节ROS/JNK信号通路在代谢相关脂肪性肝病治疗中的作用及机理研究

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现代消化及介入诊疗 2024年第6期29卷 681-690页

作者：徐惠圆李昌平刘超任涛西藏自治区人民政府驻成都办事处医院消化内科 610000 西南医科大学附属医院消化内科 646000

目的探讨益生菌通过调节ROS/JNK信号通路在代谢相关脂肪性肝病治疗中的作用和机制。方法选取18只C57BL/6雄性小鼠,随机分成模型组(n=6)、对照组(n=6)及益生菌组(n=6)。给予模型组及益生菌组小鼠MCD饮食来构建代谢相关脂肪性肝病(MAFLD)... 详细信息

目的探讨益生菌通过调节ROS/JNK信号通路在代谢相关脂肪性肝病治疗中的作用和机制。方法选取18只C57BL/6雄性小鼠,随机分成模型组(n=6)、对照组(n=6)及益生菌组(n=6)。给予模型组及益生菌组小鼠MCD饮食来构建代谢相关脂肪性肝病(MAFLD)小鼠模型,对照组小鼠喂养MCS饮食,益生菌组小鼠以益生菌灌胃,每天灌胃一次,每次0.5 mL,模型组和对照组予以等量生理盐水灌胃,连续灌胃4周。益生菌治疗4周后将小鼠麻醉处死,检测各组小鼠血清中丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、甘油三酯(TG)和胆固醇(TC)、血浆D-乳酸(D-Lac)、血清及小肠二胺氧化酶(DAO)水平变化情况。选取肝脏及回肠组织切片后行HE染色。同时检测各组小鼠肝组织中活性氧(ROS)水平。使用免疫印迹技术检测各组小鼠ROS/JNK信号通路相关蛋白表达情况,如磷酸化c-Jun氨基末端激酶(P-JNK)、B淋巴细胞瘤-2基因相关X蛋白(Bax)、紧密连接蛋白ZO-1、含半胱氨酸的天冬氨酸蛋白水解酶-3(Caspase-3)。使用免疫组化技术检测各组小鼠ROS/JNK信号通路相关蛋白表达情况,如P-JNK、Caspase-3、Bax的表达水平。使用PCR检测各组小鼠Bax、Caspase-3的mRNA表达。结果肝脏HE染色病理学结果分析:喂养C57BL/6小鼠MCD饮食4周后通过HE染色观察肝小叶结构紊乱,肝细胞内可见脂滴沉积并伴有严重的炎性细胞浸润,证明成功建立MAFLD动物模型。益生菌治疗4周后,与模型组相比,益生菌组小鼠肝小叶结构变得完整,肝细胞内脂肪变性和炎性细胞浸润减少。生化结果分析:C57BL/6小鼠喂养MCD饮食后,模型组小鼠血清中血脂(TC、TG)及转氨酶(ALT、AST)、血浆D-Lac、血清及小肠DAO和肝组织中ROS水平较对照组明显增高。而在使用益生菌治疗4周后,与模型组小鼠相比,益生菌组小鼠的血清TC、TG、ALT、AST、血浆D-Lac、血清及小肠DAO和肝脏中ROS水平均明显下降(P<0.05)。Western blot结果分析:C57BL/6小鼠喂养MCD饮食后,与对照组小鼠相比,模型组小鼠肝脏P-JNK、Caspase-3、Bax表达水平明显增加,肠道ZO-1的表达水平明显降低;而使用益生菌治疗4周后,与模型组小鼠相比,益生菌组小鼠肝脏P-JNK、Caspase-3、Bax的表达水平降低,而ZO-1的表达水平增加(P<0.05)。PCR结果分析:C57BL/6小鼠喂养MCD饮食后,模型组小鼠Bax、Caspase-3的mRNA表达水平较对照组明显增加;而益生菌治疗后,与模型组相比,益生菌组小鼠Bax、Caspase-3的mRNA表达降低(P<0.05)。免疫组化结果分析:C57BL/6小鼠喂养MCD饮食后,模型组小鼠肝脏P-JNK、Caspase-3、Bax的表达水平较对照组升高;在使用益生菌治疗后,与模型组相比,益生菌组小鼠肝脏P-JNK、Caspase-3、Bax的表达水平降低(P<0.05)。小肠HE染色病理学结果分析:对照组小鼠的回肠粘膜结构正常,无充血水肿、溃疡、剥脱。与对照组相比,模型组小鼠的回肠粘膜结构严重破坏,肠粘膜出现水肿、溃疡、剥脱现象。使用益生菌干预后,益生菌组小鼠肠粘膜结构变得较为完整,肠粘膜无明显水肿、溃疡、剥脱,见图。相较于对照组,模型组小鼠回肠黏膜Chiu氏病理评分更高。使用益生菌干预后,相较于模型组,益生菌组小鼠回肠黏膜Chiu氏病理评分降低(P<0.05)。结论ROS、P-JNK、Caspase-3、Bax在MAFLD中表达增加,表明其与MAFLD的发病有关。使用益生菌治疗后可以明显改善MAFLD的组织学及血清学相关指标。Western blot、PCR及免疫组化结果都证实益生菌能通过调节ROS/JNK信号通路相关因子表达,减轻氧化应激从而抑制细胞凋亡达到治疗MAFLD的目的。

关键词：代谢相关脂肪性肝病益生菌 ROS/JNK信号通路细胞凋亡

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桥丝式电点火头发火时间一致性试验研究

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爆破器材 2024年第4期53卷 23-27页

作者：杨刚彭星新孙热刘芳谢全民姚颖康江汉大学精细爆破国家重点实验室湖北武汉430056 中铁十一局集团第四工程有限公司湖北武汉430073 武汉爆破有限公司湖北武汉430056

为了确定桥丝式电点火头发火时间一致性的影响因素,采用高速摄影法对单个电点火头、电点火头串联组和电点火头并联组的发火过程和发火时间进行研究。试验结果表明:对于单个电点火头,发火电流超过1.4 A时,平均发火时间在5.0 ms内,继续增... 详细信息

为了确定桥丝式电点火头发火时间一致性的影响因素,采用高速摄影法对单个电点火头、电点火头串联组和电点火头并联组的发火过程和发火时间进行研究。试验结果表明:对于单个电点火头,发火电流超过1.4 A时,平均发火时间在5.0 ms内,继续增加发火电流,发火时间缩短趋势减缓;电点火头串联组的发火电流不宜低于1.8 A,发火时间差可控制在1.5 ms内;电点火头的发火时间一致性,并联组明显优于串联组。在进行组网爆破作业时,对于内置电点火头的一次性膨胀爆破管,推荐采用并联组网,普通电(220 V)可将电点火头的发火时间差控制在0.1 ms以内。

关键词：电点火头发火时间串联组网并联组网一致性

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Fracture toughness and destructive mechanism of ductile nanoporous metallic glass and its crystal-impregnated nanocomposite

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Science China(Technological Sciences) 2023年第11期66卷 3328-3342页

作者： ZHANG YuHang XU JianFei HU YiQun SU Lei DING SuHang WU WenWang XIA re Key Laboratory of Hydraulic Machinery Transients(Wuhan University) Ministry of EducationWuhan 430072China Department of Engineering Mechanics School of Naval ArchitectureOcean&Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Nanoporous metallic glasses(NPMGs)and crystalline/amorphous nanocomposites exhibit superior ductility over bulk macroscopic metallic glasses(MGs).Their fracture behaviors remain a mystery due to experimental technical *** this work,the fracture behaviors of pre-cracked NPMGs and crystal-impregnated nanoporous metallic glasses(CINPMGs)are investigated through large-scale molecular dynamics simulations,and the MG and crystal phases are amorphous Cu_(50)Zr_(50)and crystalline B2CuZr,*** toughness is determined by simultaneously considering the surface energy and plastic dissipated *** results confirm the excellent plasticity of both the pre-cracked NPMGs and *** progressive necking and ductile rupture of ligaments are responsible for generating NPMGs with prominent ductility and fracture ***,secondary cracking is also triggered,which can consume energy without extending the major crack,thus further improving fracture *** is also found that the fracture toughness of NPMGs can be improved by increasing the solid fraction of MG,and linear relation between fracture toughness and solid fraction can be *** impregnation effectively inhibits global failure as the crystal phase shields the individual amorphous *** plastic flow characterized by shear bands is observed in CINPMGs,and this homogeneous global deformation is facilitated by the crystalline/amorphous ***,the fracture toughness of CINPMGs is higher than that of the constituent single phases,regardless of the volume fraction of each *** material charts manifest that these two types of materials demonstrate promising potential in the material selection library for advanced structural design.

关键词： nanoporous metals crack fracture toughness nanocomposites metallic glasses

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Mechanics and Wave Propagation Characterization of Chiral S-Shaped Auxetic Metastructure

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Acta Mechanica Solida Sinica 2022年第4期35卷 571-586页

作者： Qingsong Zhang Wenjie Hong Jianfei Xu Yuhang Zhang Suhang Ding Wenwang Wu re Xia Key Laboratory of Hydraulic Machinery Transients(Wuhan University) Ministry of EducationWuhan430072China School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China Institute of Advanced Structure Technology Beijing Institute of TechnologyBeijing100081China Hubei Key Laboratory of Waterjet Theory and New Technology Wuhan UniversityWuhan430072China

Auxetic metastructures have attracted tremendous attention because of their robust multifunctional properties and promising potential industrial *** paper studies the in-plane mechanical behaviors of a chiral S-shaped metastructure subjected to tensile loading in both X-direction and Y-direction and wave propagation properties using the finite element(FE)*** relationships between structural parameters and elastic behaviors are also *** results indicate that the orientation of chiral S-shaped metastructure under tensile loading in the X-direction exhibits higher auxeticity and ***,the band structures and the edge modes of each band gap of the chiral S-shaped metastructure are explored,and the relations between band gap properties and structural parameters are also systematically ***,we explore the wave mitigation of the chiral S-shaped metastructures by regulating the structural ***,the transmission properties of the finite chiral S-shaped periodic metastructures are studied to confirm the results of band gap *** study promotes the engineering application of vibration isolation of chiral structures based on the band gap theory.

关键词： Auxetic metastructures Mechanical properties Band gaps Wave mitigation

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Randomized controlled trial of minimally invasive surgery using acellular dermal matrix for complex anorectal fistula

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World Journal of Gastroenterology 2010年第26期16卷 3279-3286页

作者： Ma-Mu-Ti-Jiang A ba-bai-ke-re Er-Ha-Ti Ai Anorectal Surgical Department First Aff iliated Hospital of Xinjiang Medical University

AIM: To compare the efficacy and safety of acellular dermal matrix (ADM) bioprosthetic material and endorectal advancement flap (ERAF) in treatment of complex anorectal fistula. METHODS: Ninety consecutive patients with complex anorectal fistulae admitted to Anorectal Surgical Department of First Affi liated Hospital, Xinjiang Medical University from March 2008 to July 2009, were enrolled in this study. Complex anorectal fistula was diagnosed following its clinical, radiographic, or endoscopic diagnostic criteria. Under spinal anesthesia, patients underwent identification and irrigation of the fistula tracts using hydrogen peroxide. ADM was securely sutured at the secondary opening to the primary opening using absorbable suture. Outcomes of ADM and ERAF closure werecompared in terms of success rate, fecal incontinence rate, anorectal deformity rate, postoperative pain time, closure time and life quality score. Success was defined as closure of all external openings, absence of drainage without further intervention, and absence of abscess formation. Follow-up examination was performed 2 d, 2, 4, 6, 12 wk, and 5 mo after surgery, respectively. reSULTS: No patient was lost to follow-up. The overall success rate was 82.22% (37/45) 5.7 mo after surgery. ADM dislodgement occured in 5 patients (11.11%), abscess formation was found in 1 patient, and fistula recurred in 2 patients. Of the 13 patients with recurrent fistula using ERAF, 5 (11.11%) received surgical drainage because of abscess formation. The success rate, postoperative pain time and closure time of ADM were significantly higher than those of ERAF (P rence was observed in fecal incontinence rate and anorectal deformity rate after treatment with ADM and ERAF. CONCLUSION: Closure of fistula tract opening with ADM is an effective procedure for complex anorectal fistula. ADM should be considered a first line treatment for patients with complex anorectal fistula.

关键词： Acellular dermal matrix Surgery Transsphincteric complex fistula

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Peripheral nerve regeneration and intraneural revascularization

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Neural regeneration research 2019年第1期14卷 24-33页

作者： Martial Caillaud Laurence Richard Jean-Michel Vallat Alexis Desmoulière Fabrice Billet University of Limoges Myelin Maintenance and Peripheral Neuropathies Faculties of Medicine and Pharmacy University Hospital of Limoges Department of Neurology "Reference Center for Rare Peripheral Neuropathies" Department of Neurology

Peripheral nerves are particularly vulnerable to injuries and are involved in numerous pathologies for which specific treatments are lacking. This review summarizes the pathophysiological features of the most common traumatic nerve injury in humans and the different animal models used in nerve regeneration studies. ~Ihe current knowledge concerning Wallerian degeneration and nerve regrowth is then described. Finally, the involvement of intraneural vascularization in these processes is addressed. As intraneural vascularization has been poorly studied, histological experiments were carried out from rat sciatic nerves damaged by a glycerol injection. The results, taken together with the data from literature, suggest that revascularization plays an important role in peripheral nerve regeneration and must therefore be studied more carefully.

关键词： compression crush transection Sunderland's classification Wallerian degeneration angiogenesis traumatic glycerol

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A multi-agent system for itinerary suggestion in smart environments

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CAAI Transactions on Intelligence Technology 2021年第4期6卷 377-393页

作者： Alessandra De Paola Salvatore Gaglio Andrea Giammanco Giuseppe Lo re Marco Morana Universita degzi studi di Palermo PalermoItaly Srart Cities and Comtnunitics Natioral Jab CINI-Consorzio Interuniversitario Nazionale per l'Infortnatica PalerinoItaly

Modem smart environments pose several challenges,among which the design of intelligent algorithms aimed to assist the *** a variety of points of interest are available,for instance,trajectory recommendations are needed to suggest users the most suitable itineraries based on their interests and contextual *** in many cases,these interests must be explicitly requested and their lack causes the so-called cold-start ***,lengthy travelling distances and excessive crowdedness of specific points of interest make itinerary planning more *** address these aspects,a multi-agent itinerary suggestion system that aims at assisting the users in an online and collaborative way is proposed.A profiling agent is responsible for the detection of groups of users whose movements are characterised by similar semantic,spatial and temporal features;then,a recommendation agent leverages contextual information and dynamically associates the current user with the trajectory clusters according to a Multi-Armed Bandit policy;Framing the trajectory recommendation as a reinforcement learning problem permits to provide high-quality suggestions while avoiding both cold-start and preference elicitation *** effectiveness of the approach is demonstrated by some deployments in real-life scenarios,such as smart campuses and theme parks.

关键词： artificial intelligence pattern recognition

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Profile of Full-Term Births in Maternity Wards of Public Hospitals in Douala Cameroon

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Open Journal of Obstetrics and Gynecology 2024年第5期14卷 705-720页

作者： Henri Essome Merlin Boten Bounyom Astrid Ndolo Kondo Ingrid Doriane Ofakem Ilick Fulbert Mangala Nkwele Irène Cyrielle Edjoa Mboe Michel Roger Ekono Alphonse Ngalame Nyong Robert Tchounzou Moustapha Bilkissou Junie Ngaha Yaneu Marga Vanina Ngono Akam Gervais Mounchikpou Ngouhouo Grâce Tocki Toutou Théophile Nana Njamen Valère Mve Koh Pascal Foumane Faculty of Medicine and Pharmaceutical Sciences University of Douala Douala Cameroon Laquintinie Hospital Douala Cameroon Faculty of Health Sciences University of Bamenda Bamenda Cameroon Faculty of Health Sciences University of Buea Buea Cameroon Faculty of Medicine and Biomedical Sciences University of Yaound&#233 1 Yaound&#233 Cameroon Faculty of Medicine and Pharmaceutical Sciences of Sangmelima University of Ebolowa Ebolowa Cameroon

Introduction: Pregnancy as much as childbirth constitutes a risky situation, potentially fraught with sometimes dramatic complications: maternal death. Objective: We conducted this study with the aim of establishing the profile of those giving birth in our context with the aim to anticipate operationally in the future on morbidity but more on maternal deaths. Methodology: We conducted, using a structured questionnaire, a prospective descriptive study in representative maternity wards in the city of Douala;the study variables were socio-economic, anthropometric, obstetrical and clinical. Statistical analyses were carried out with CS Pro 7.3 and SPSS version 25.0 software. The Student, Chi-square and Fischer tests were used to compare the means of the variables and the percentages. results: We recruited 305 births for our study. The average age of our births was 28.7 years ± 6.1 with an average height of 161.6 cm ± 5.06;an average body mass index at the start of pregnancy of 28.0 kilograms/square meter and 31.3 kilograms/square meter at delivery;the average weight gain was 8.4 g ± 5.37;an average gestation of 2.84±1.90;an average parity of 2.2 ± 2.1 with an average birth interval of 27.7 months ± 23.7. The average gestational age was 39.2 weeks ± 1.21 with pregnancy pathology dominated by malaria;85.9% began their prenatal follow-up before the 14th week of amenorrhea. Conclusion: The profile of childbirth in urban Cameroon does not seem potentially dystocic compared to that of the same regional and racial area.

关键词： Profile Delivery Term Malaria Douala

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浅埋煤层烧变岩地球化学与变质矿物相特征

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西北大学学报（自然科学版） 2024年第2期54卷 329-344页

作者：杨帆热西提·亚力坤薛小渊宋世骏胡俭张泽宇姬中奎黄雷陕西煤业化工集团神木张家峁矿业有限公司陕西神木719316 西北大学地质学系/大陆动力学国家重点实验室陕西西安710069 中国煤炭科工西安研究院〔集团〕有限公司陕西西安710054 陕西省煤矿水害防治技术重点实验室陕西西安710077

以陕北张家峁井田延安组2^(2),3^(1)段烧变岩为研究对象,对其进行岩石地球化学迁移规律以及变质矿物相特征分析。结果表明,该区烧变岩为低碱性硅铝质变质岩,横向上钙碱性分布不均一,少部分烧变样品烧失量大且CaO含量高,Fe元素迁移聚集... 详细信息

以陕北张家峁井田延安组2^(2),3^(1)段烧变岩为研究对象,对其进行岩石地球化学迁移规律以及变质矿物相特征分析。结果表明,该区烧变岩为低碱性硅铝质变质岩,横向上钙碱性分布不均一,少部分烧变样品烧失量大且CaO含量高,Fe元素迁移聚集。Zr相容性比Hf更好,Ba元素富集可能与后期水文作用有关,稀土元素分配趋势线为略微轻稀土富集型,烧变作用对稀土元素的分配影响相对有限。烧变岩原生矿物多具有烧蚀特征,镜下发育烧蚀边、气孔构造等,新生矿物呈集合体充填矿物间或矿物裂缝中。矿物相组合与热接触变质作用中发生高热变质的透长岩相相近,但变质压力更低,温度更高。烧变岩层由下而上可分为薄层板状烧烤岩、烧熔岩、厚层板状烧烤岩、厚层层状烧烤岩的岩石组合,鳞石英+方石英相较莫来石+堇青石+赤铁矿能更好表征岩石烧变程度的强弱,同层烧烤岩下部烧变程度高于上部,烧熔岩上部板状烧烤岩烧变程度高于下部板状烧烤岩。研究区烧变岩具有“隐伏”的特点,且存在同沉积期烧变作用,除暴露在河谷阶地的烧变岩层外,部分烧变岩层埋于更新世地层和残余延安组地层之下。早期形成的烧变岩层后期被破坏,多期烧变地层相互叠置形成现今火烧区。

关键词：烧变岩张家峁井田地球化学变质矿物相热接触变质作用透长岩相

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