Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

作     者：J.E.Hernández-Pichardo Y.Ducolomb S.Romo M.E.Kjelland R.Fierro F.Casillas M.Betancourt 

作者机构：División de Ciencias Biológicas y de la SaludDepartamento de Producción Agrícola y AnimalUniversidad Autónoma Metropolitana-XochimilcoCiudad de MexicoMexico Division de Ciencias Biologicas y de la SaludDepartamento de Ciencias de la SaludUniversidad Autónoma Metropolitana-IztapalapaSan Rafael Atlixco 186CP 09340 Ciudad de MexicoMexico Departamento de Ciencias PecuariasFacultad de EstudiosSuperiores CuautitlanUNAMCiudad de MexicoEstado de MexicoMexico ConservationGenetics&BiotechLLCValley CityNDUSA Division de Ciencias Biologicas y de la SaludDepartamento de Biologia de la ReproduccionUniversidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico Doctorado en Biologia ExperimentalUniversidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2017年第8卷第2期

页      面：370-377页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

主　　题：Ethanol ICSI Oocyte activation Ovine Pronucleus Zona pellucida 

摘      要：Background： In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically activated ovine oocytes and sperm selected by swim up（SU） or swim up ＋ zona pellucida（SU ＋ ZP） ***： Experiment 1, 4–20 replicates with total 821 in vitro matured oocytes were chemically activated with ethanol, calcium ionophore or ionomycin, to determine oocyte activation（precense of one PN）. Treatments showed similar results（54, 47, 42 %, respectively） but statistically differents（P 0.05）.Conclusions： Chemical activation induces higher ovine oocyte activation than mechanical activation. Ethanol slightly displays higher oocyte activation than calcium ionophore and ionomicine. Sperm selection with SU ＋ ZP increased AR/A and AR/D rates in comparison with SU in fresh and frozen-thawed sperm. According to this, in terms of fertilization rates, chemical activation after ICSI increased oocyte PN formation compared to mechanical activation. Also, fresh sperm treated with SU and SU ＋ ZP were significantly different than frozen-thawed sperm,but between sperm treatments no significant differences were obtained.