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基因重组/尿卵泡刺激素对卵巢的反应及辅助生殖技术结局的对比性研究

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宁夏医学杂志 2005年第10期27卷 651-654页

作者：徐仙 rafael a.cabrera Benjamin C.Wong 宁夏医学院附属医院宁夏银川750004 美国弗吉尼亚Jones生殖医学研究院弗吉尼亚诺福克23507

目的评估基因重组和尿卵泡刺激素对不孕妇女卵巢的刺激反应和体外受精(IVF)或卵胞浆内单精子注射(ICSI)的妊娠结局。方法对1049例实施IVF或ICSI的不孕患者作回顾性分析,在2种促性腺激素释放激素激动剂(GnRHa)方案下,分别给予尿卵泡刺激... 详细信息

目的评估基因重组和尿卵泡刺激素对不孕妇女卵巢的刺激反应和体外受精(IVF)或卵胞浆内单精子注射(ICSI)的妊娠结局。方法对1049例实施IVF或ICSI的不孕患者作回顾性分析,在2种促性腺激素释放激素激动剂(GnRHa)方案下,分别给予尿卵泡刺激素(uFSH)、高纯度尿卵泡刺激素(hpFSH)和基因重组卵泡刺激素(rFSH)促排卵,比较3组雌二醇(E2)峰值水平、FSH剂量、成熟卵母细胞数、受精率和妊娠率。结果在中度和高度卵巢反应者(GnRHa长方案)、hpFSH组和rFSH组出现较高的E2峰值和绒毛膜促性腺素的注射延迟,其中rFSH组的胚胎种植率和持续妊娠率最高;在低弱卵巢反应者(GnRHa短方案)、hpFSH组和rFSH组的E2峰值显著增高,其周期取消率也明显减低,但各组之间种植率或妊娠率无明显差异。结论本研究说明,在卵巢的反应性和IVF/IC-SI妊娠结局的层面上,基因重组卵泡刺激素较尿卵泡刺激素更显优越性。

关键词：基因重组卵泡刺激素尿卵泡刺激素卵巢反应性妊娠

Typhoon-and pollution-driven enhancement of reactive bromine in the mid-latitude marine boundary layer

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National Science Review 2024年第4期11卷 93-101页

作者： Shanshan Wang Qinyi Li Ruifeng Zhang Anoop Sharad Mahajan Swaleha Inamdar Nuria Benavent Sanbao Zhang Ruibin Xue Jian Zhu Chenji Jin Yan Zhang Xiao Fu Alba Badia rafael P.Fernandez Carlos A.Cuevas Tao Wang Bin Zhou Alfonso Saiz-Lopez Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3) Department of Environmental Science and Engineering Fudan University Institute of Eco-Chongming(IEC) Department of Atmospheric Chemistry and Climate Institute of Physical Chemistry Blas CabreraCSIC Department of Civil and Environmental Engineering The Hong Kong Polytechnic University Environment Research Institute Shandong University Centre for Climate Change Research Indian Institute of Tropical MeteorologyMinistry of Earth Sciences Department of Chemistry University of Colorado Boulder Institute of Environment and Ecology Tsinghua Shenzhen International Graduate SchoolTsinghua University Sostenipra Research Group Institute of Environmental Science and Technology(ICTA)Universitat Autonoma de Barcelona(UAB) Institute for Interdisciplinary Science (ICB) National Research Council (CONICET)

Tropospheric reactive bromine is important for atmospheric chemistry,regional air pollution,and global *** studies have reported measurements of atmospheric reactive bromine species in different environments,and proposed their main sources,***-salt aerosol（SSA）,oceanic biogenic activity,polar snow/ice,and *** and other strong cyclonic activities（***） induce abrupt changes in different earth system processes,causing widespread destructive ***,the role of typhoons in regulating reactive bromine abundance and sources remains ***,we report field observations of bromine oxide（BrO）,a critical indicator of reactive bromine,on the Huaniao Island（HNI）in the East China Sea in July *** observed high levels of BrO below 500 m with a daytime average of9.7±4.2 pptv and a peak value of ～26 pptv under the influence of a *** field measurements,supported by model simulations,suggest that the typhoon-induced drastic increase in wind speed amplifies the emission of SSA,significantly enhancing the activation of reactive bromine from SSA *** also detected enhanced BrO mixing ratios under high NOxconditions（ppbv level） suggesting a potential pollution-induced mechanism of bromine release from *** elevated levels of atmospheric bromine noticeably increase ozone destruction by as much as ～40% across the East China *** the high frequency of cyclonic activity in the northern hemisphere,reactive bromine chemistry is expected to play a more important role than previously thought in affecting coastal air quality and atmospheric oxidation *** suggest that models need to consider the hitherto overlooked typhoon-and pollution-mediated increase in reactive bromine levels when assessing the synergic effects of cyclonic activities on the earth system.

关键词： reactive bromine atmospheric chemistry marine boundary layer atmospheric oxidation capacity marine emission

Influence of birth order, birth weight, colostrum and serum immunoglobulin G on neonatal piglet survival

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Journal of Animal Science and Biotechnology 2013年第2期4卷 128-136页

作者： rafael a.cabrera Xi Lin Joy M Campbell Adam J Moeser Jack Odle Laboratory of Developmental Nutrition Department of Animal Science North Carolina State University Huvepharma USA Inc. 525 West Park Drive Suite 230 Peachtree City American Protein Corporation Department of Population Health and Pathobiology College of Veterinary Medicine North Carolina State University 101 Polk Hall North Carolina State University

Background： Intake of colostrum after birth is essential to stimulate intestinal growth and function, and to provide systemic immunological protection via absorption of Immunoglobulin G （IgG）. The birth order and weight of 745 piglets （from 75 litters） were recorded during a one-week period of farrowing. Only pigs weighing greater than 0.68 kg birth weight were chosen for the trial. Sow colostrum was collected during parturition, and piglets were bled between 48 and 72 hours post-birth. Piglet serum IgG and colostral IgG concentrations were determined by radial immunodiffusion. Results： Sow parity had a significant （P 〈 0.001） effect on sow colostral IgG concentration, being 5% higher in multiparous females. Sow colostral IgG concentration explained 6% and piglet birth order accounted for another 4% of the variation observed in piglet serum IgG concentration （P 〈 0.05）; however, birth weight had no detectable effect. Piglet serum IgG concentration had both a linear （P 〈 0.05） and quadratic effect （P 〈 0.05） on % survival. Piglets with 1,000 mg/dl serum IgG or less （n=24） had a 67% survival; whereas, piglets with IgG concentrations between 2250 to 2500 mg/dl （n=247） had a 91% survival. Birth order had no detectable effect on survival, but birth weight had a positive linear effect （P 〈 0.05）. Piglets weighing 0.9 kg （n = 107） at birth had a 68% survival rate, and those weighing 1.6 kg （n = 158） had an 89% survival. Conclusion： We found that the combination of sow colostrum IgG concentration and birth order can account for 10% of the variation of piglet serum IgG concentration and that piglets with less than 1,000 mg/dl IgG serum concentration and weight of 0.9 kg at birth had low survival rate when compared to their larger siblings. The effective management of colostrum uptake in neonatal piglets in the first 24 hrs post-birth may potentially improve survival from birth to weaning.

关键词： Birth order Birth weight Immunoglobulin G Colostrum Survivability

Effects of creep feeding and supplemental glutamine or glutamine plus glutamate (Aminogut) on pre- and post-weaning growth performance and intestinal health of piglets

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Journal of Animal Science and Biotechnology 2013年第3期4卷 211-222页

作者： rafael a.cabrera James L Usry Consuelo Arrellano Eduardo T Nogueira Marianne Kutschenko Adam J Moeser Jack Odle Laboratory of Developmental Nutrition Department of Animal ScienceNorth Carolina State University 101 Polk Hall North Carolina State UniversityRaleigh NC 27695 USA Huvepharma USA 525 West Park Drive Suite 230 Peachtree City GA 30269 USA Ajinomoto Heartland Lysine Department of Statistics North Carolina State UniversityRaleigh NC 27695-8203 USA Ajinomoto do Brasil. Ajinomoto Animal Nutrition Sāo Paulo SP 04015-001 Brazil Department of Population Health and Pathobiology College of Veterinary Medicine North Carolina State UniversityRaleigh NC 27695 USA Laboratory of Developmental Nutrition Department of Animal ScienceNorth Carolina State University 101 Polk Hall North Carolina State UniversityRaleigh NC 27695 USA

Background： Creep feeding is used to stimulate piglet post-weaning feed consumption.L-Glutamine（GLN） is an important source of fuel for intestinal epithelial *** objective of this study was to determine the impact of creep feeding and adding GLN or AminoGut（AG;containing glutamine ＋ glutamate） to pre-and post-weaning diets on pig performance and intestinal ***（N = 120） were allotted to four treatments during 14–21 d of lactation： 1） No creep feed（NC,n = 45）;2） creep fed control diet（CFCD,n = 45）;3） creep fed 1% GLN（CFGLN,n = 15）;4） creep fed.88% AG（CFAG,n = 15）.After weaning,the NC and CFCD groups were sub-divided into three groups（n = 15 each）,receiving either a control nursery diet（NC-CD,CFCD-CD） or a diet supplemented with either GLN（NC-GLN,CFCD-GLN） or with AG（NC-AG,CFCD-AG）.Litters that were creep fed with diets containing GLN or AG also were supplemented with those amino acids in the nursery diets（CFGLN-GLN,CFAG-AG）.Glutamine was added at 1% in all three post-weaning diet phases and AG was added at.88% in phase 1 and 2 and at.66% in phase ***： Feed conversion（feed/gain） showed means among treatment means close to significance（P = 0.056） and Tukey＇s test for pairwise mean comparisons showed that Pigs in the CFGLN-GLN group had the best feed conversion（feed/gain） in the first three-week period post-weaning,exceeding（P = 0.044） controls（CFCD-CD） by 34%.The NC-AG group had（P = 0.02） the greatest feed intake in the last three week of the study,exceeding controls（CFCD-CD） by 12%.CFGLN-GLN,CFCD-GLN and sow reared（SR） pigs had the greatest（P = 0.049） villi height exceeding the CFCD-AG group by 18%,20% and 19% *** CFAG-AG group had the deepest（P = 0.001） crypts among all ***-GLN,CFCD-GLN and SR groups had the greatest（P = 0.001） number of cells proliferating（PCNA） exceeding those in the NC-CD group by 43%,54% and 63% *** reared pigs showed the greatest（P = 0.001） intestinal absorption capacity for xylose and

关键词： Creep feeding Glutamine Growth performance Intestinal health SEM Villi

Fault diagnosis of spur gearbox based on random forest and wavelet packet decomposition

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Frontiers of Mechanical Engineering 2015年第3期10卷 277-286页

作者： Diego cabrera Fernando SANCHO Rene-Vinicio SANCHEZ Grover ZURITA Mariela CERRADA Chuan LI rafael E. VASQUEZ Departamento de Ingenieria Mecainica Universidad Politecnica Salesiana Cuenca Ecuador Departamento de Ciencias de la Computaci6n e Inteligencia Artificial Universidad de Sevilla Espafia Departamento de Ingenieria Electro-Mechnica Universidad PrivadaBoliviana Cochabamba Bolivia Departamento de Sistemas de Control Universidad de Los AndesM6rida Venezuela Research Center of System Health Maintenance Chongqing Technologyand Business University Chongqing 400067 China Facultad de Ingenieria Mecainica Universidad Pontificia BolivarianaMedellln Colombia

This paper addresses the development of a random forest classifier for the muki-class fault diagnosis in spur gearboxes. The vibration signal＇s condition parameters are first extracted by applying the wavelet packet decomposition with multiple mother wavelets, and the coefficients＇ energy content for terminal nodes is used as the input feature for the classification problem. Then, a study through the parameters＇ space to find the best values for the number of trees and the number of random features is performed. In this way, the best set of mother wavelets for the application is identified and the best features are selected through the internal ranking of the random forest classifier. The results show that the proposed method reached 98.68% in classification accuracy, and high efficiency and robustness in the models.

关键词： fault diagnosis spur gearbox wavelet packet decomposition random forest

High pressure effects on the excitation spectra and dipole properties of Li, Be+, and B2+ atoms under confinement

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Matter and Radiation at Extremes 2020年第2期5卷 43-52页

作者： C.Martinez-Flores R.cabrera-Trujillo Departamento de Qu´ımica Universidad Aut´onoma Metropolitana IztapalapaSan Rafael Atlixco 186Col.VicentinaIztapalapaC.P.09340 M´exico D.F.Mexico Instituto de Ciencias F´ısicas Universidad Nacional Aut´onoma de M´exicoAp.Postal 43-8CuernavacaMorelos62251Mexico Theoretische Chemie Physikalisch-Chemisches InstitutUniversit¨at HeidelbergINF 22969120 HeidelbergGermany

Properties of atoms and molecules undergo significant changes when subjected to spatial *** study the excitation spectra of lithiumlike atoms in the initial 1s22s electronic configuration when confined by an impenetrable spherical *** implement Slater’s X-αmethod in Hartree–Fock theory to obtain the excitation *** that as the cavity size decreases,the total,2s,2p,and higher excited energy levels ***,we confirm the existence of crossing points between ns–np states for low values of the confinement radius such that the ns→np dipole transition becomes zero at that critical *** crossing points of the s–p states imply that instead of photon absorption,one observes photon emission for cavities with radius smaller than the critical ***,the dipole oscillator strength associated with the 2s→2p transition becomes negative,and for higher pressures,the 2s→3p dipole oscillator strength transition becomes larger than *** validate the completeness of the spectrum by calculating the Thomas–Reiche–Kuhn sum rule,as well as the static dipole polarizability and mean excitation energy of lithium-like *** find that the static dipole polarizability decreases and exhibits a sudden change at the critical pressure for the absorption-to-emission *** mean excitation energy increases as the pressure ***,as a consequence of the critical transition fromabsorption to emission,themean excitation energy becomes undetermined for higher pressures,with implications formaterial damage under extreme *** unconfined systems,our results show good to excellent agreement with data found in the literature.

关键词： transition. excitation dipole

SARS-CoV-2 spike RBD-specific IgA and IgG antibodies in breast milk after vaccination with the protein subunit vaccine Abdala

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Infectious Medicine 2022年第4期1卷 253-261页

作者： Maylin Pérez-Bernal Carlos Hernández rafael Ibargollín Midalis Martínez Migdiala Soria Magali Delgado Onel Valdivia DayamíDorta Andy Domínguez Enrique Pérez Yeosvany cabreraa Research&Development Department Center for Genetic Engineering and BiotechnologyCircunvalante NorteOlivos IIISancti SpiritusCuba Neonatology Service General Hospital“Camilo Cienfuegos”128 Bartolome MasoSancti SpiritusCuba Production Department Center for Genetic Engineering and BiotechnologyCircunvalante NorteOlivos IIISancti SpiritusCuba

Background:COVID-19 vaccines that trigger a strong secretory antibody response in breast milk may achieve effective passive protection of vulnerable newborns and breastfed infants of immunized *** aim of this work was to investigate the presence of SARS-CoV-2 spike RBD-specific IgA and IgG antibodies in breast milk,5 and 9 weeks after vaccination with 3 doses of the protein subunit vaccine Abdala,compared to those found in breast milk from COVID-19-recovered women,collected at least 40 days after the ***:SARS-CoV-2 spike RBD-specific IgA and IgG antibodies were semi-quantified by indirect ELISA,using a homemade standard generated by pooling twenty breast milk samples with high absorbance values according to preliminary *** validity of the standard curves was proved following the European Medicines Agency *** breast milk samples from 2 unvaccinated women who had not been infected with COVID-19 were included as negative *** neutralizing antibodies was assessed by a SARS-CoV-2 surrogate virus neutralization ***:High levels of anti-RBD IgA antibodies were detected in breast milk samples 9 weeks after vaccination and anti-RBD IgG antibodies rise from the fifth to the ninth *** the post-COVID-19 time that was evaluated,the IgG-type response was notably higher compared to both post-vaccination *** antibody titers were similar in breast milk from vaccinated and COVID-19 recovered ***:This is the first report about the immune response in breast milk after the administration of a COVID-19 protein subunit vaccine,which could provide analogous protection to that conferred by SARS-CoV-2 *** implies a potential passive immunity that breastfed infants receive from their mothers vaccinated with Abdala.

关键词： Abdala vaccine IgA IgG Neutralizing antibody titers SARS-CoV-2

Nutraceutical Effect of Cuetlas （Arsenura armida C.） Edible Insects as Local Food at Ixcaquixtla, Mexico

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Journal of Agricultural Science and Technology(A) 2016年第6期6卷 423-428页

作者： Virginia Melo-Ruiz Tomas Quirino-Barreda Susana Macin-cabrera Karina Sanchez-Herrera rafael Diaz-Garcia Cesar Gazga-Urioste Department of Biologic Systems Metropolitan Autonomous University Mexico City PC 04960 Mexico Department of Health Care Metropolitan Autonomous University Mexico City PC 04960 Mexico

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乳代用品对仔猪生长的影响

国外畜牧学（猪与禽） 2007年第4期27卷 4-6页

作者：方正锋（译）方如君（校） rafael cabrera 不详美国明尼苏达州Marshall Ralco营养公司

如果管理得当，给哺乳期的仔猪补充乳可获得多方面的好处。为获得高断奶窝重而对母猪进行高繁殖性能和高营养需求的选择已使饲喂现代的母猪品种成为商业化养猪场中技术最先进的工作。如果青年母猪要达到200～250kg体重时它才算完全体成... 详细信息

如果管理得当，给哺乳期的仔猪补充乳可获得多方面的好处。为获得高断奶窝重而对母猪进行高繁殖性能和高营养需求的选择已使饲喂现代的母猪品种成为商业化养猪场中技术最先进的工作。如果青年母猪要达到200～250kg体重时它才算完全体成熟，那么当体重为125～145kg时就进行配种以及动员体内储备哺乳10～11头仔猪的事实已经成为青年母猪的一个沉重负担。

关键词：仔猪生长哺乳期代用品青年母猪营养需求繁殖性能断奶窝重养猪场