SARS-CoV-2 spike RBD-specific IgA and IgG antibodies in breast milk after vaccination with the protein subunit vaccine Abdala

作     者：Maylin Pérez-Bernal Carlos Hernández Rafael Ibargollín Midalis Martínez Migdiala Soria Magali Delgado Onel Valdivia DayamíDorta Andy Domínguez Enrique Pérez Yeosvany Cabreraa Maylin Pérez-Bernal;Carlos Hernández;Rafael Ibargollín;Midalis Martínez;Migdiala Soria;Magali Delgado;Onel Valdivia;Dayamí Dorta;Andy Domínguez;Enrique Pérez;Yeosvany Cabrera

作者机构：Research&Development DepartmentCenter for Genetic Engineering and BiotechnologyCircunvalante NorteOlivos IIISancti SpiritusCuba Neonatology ServiceGeneral Hospital“Camilo Cienfuegos”128 Bartolome MasoSancti SpiritusCuba Production DepartmentCenter for Genetic Engineering and BiotechnologyCircunvalante NorteOlivos IIISancti SpiritusCuba 

出 版 物：《Infectious Medicine》 (感染医学（英文）)

年 卷 期：2022年第1卷第4期

页      面：253-261页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Abdala vaccine IgA IgG Neutralizing antibody titers SARS-CoV-2 

摘      要：Background:COVID-19 vaccines that trigger a strong secretory antibody response in breast milk may achieve effective passive protection of vulnerable newborns and breastfed infants of immunized mothers.The aim of this work was to investigate the presence of SARS-CoV-2 spike RBD-specific IgA and IgG antibodies in breast milk,5 and 9 weeks after vaccination with 3 doses of the protein subunit vaccine Abdala,compared to those found in breast milk from COVID-19-recovered women,collected at least 40 days after the infection.Methods:SARS-CoV-2 spike RBD-specific IgA and IgG antibodies were semi-quantified by indirect ELISA,using a homemade standard generated by pooling twenty breast milk samples with high absorbance values according to preliminary data.The validity of the standard curves was proved following the European Medicines Agency Guideline.Two breast milk samples from 2 unvaccinated women who had not been infected with COVID-19 were included as negative controls.Potentially neutralizing antibodies was assessed by a SARS-CoV-2 surrogate virus neutralization test.Results:High levels of anti-RBD IgA antibodies were detected in breast milk samples 9 weeks after vaccination and anti-RBD IgG antibodies rise from the fifth to the ninth week.In the post-COVID-19 time that was evaluated,the IgG-type response was notably higher compared to both post-vaccination periods.Neutralizing antibody titers were similar in breast milk from vaccinated and COVID-19 recovered women.Conclusions:This is the first report about the immune response in breast milk after the administration of a COVID-19 protein subunit vaccine,which could provide analogous protection to that conferred by SARS-CoV-2 infection.This implies a potential passive immunity that breastfed infants receive from their mothers vaccinated with Abdala.