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Journal of Environmental Sciences 2014年第8期26卷 1689-1697页

作者： Deyong Kong Bin Liang Duu-Jong Lee Aijie Wang nanqi ren State Key Laboratory of Urban Water Resource and Environment School of Municipal and Environmental EngineeringHarbin Institute of Technology Key laboratory of Environmental Biotechnology Research Center for Eco-Environmental SciencesChinese Academy of Sciences

Exposure to chloramphenicol（CAP）,a chlorinated nitroaromatic antibiotic,can induce CAP-resistant bacteria/genes in diverse environments. A biocathode bioelectrochemical system（BES） was applied to reduce CAP under switched operational *** switching from 25 to 10°C,the CAP reduction rate（kCAP） and the maximum amount of the dechlorinated reduced amine product（AMCl,with no antibacterial activity） by the biocathode communities were both markedly decreased. The acetate and ethanol yield from cathodophilic microbial glucose fermentation（with release of electrons） was also reduced. Formation of the product AMCl was enhanced by the biocathode dechloridation reaction compared with that produced from pure electrochemical or microbial dechloridation processes. The electrochemical and morphological analyses of cathode biofilms demonstrated that some cathodophilic microbes could adapt to low temperature and play a key role in CAP degradation. The resilient biocathode BES has a potential for the treatment of CAP-containing wastewater in temperature fluctuating environments.

关键词： Chloramphenicol degradation Biocathode Dechloridation Temperature switchover

Levels, distributions, and source identification of organochlorine pesticides in the topsoils in Northeastern China

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Journal of Environmental Sciences 2009年第10期21卷 1386-1392页

作者： WANG Xu ren nanqi QI Hong MA Wanli LI Yifan International Joint Research Center for Persistent Toxic Substances (IJRC-PTS) State Key Laboratory of Urban Water Resource and Environment (SKLUWRE) Harbin Institute of Technology Harbin 150090 China. Science and Technology Branch Environment Canada Toronto Ontario M3H 5T4 Canada

Seventeen topsoil samples （9 urban, 4 suburban, 3 rural and 1 background） were collected in/around Harbin, a typical city in northeast of China, to measure concentration levels of organocholrine pesticides （OCPs） in topsoil of Northeastern China in 2006. Hexachlorohexanes （HCH）, dichlorodiphenyltrichloroethane （DDT）, and hexachlorobenzene （HCB） were detected in soil samples with mean concentrations （in pg/g dry weight （dw）） of 7120, 5425, and 1039, respectively. The mean concentrations for other OCPs were very low, 4.8 pg/g dw for chlordane and 3.3 pg/g dw for endosulfan. Source identification analysis reveals that all OCPs found in soil samples were due to historical use of these chemicals or from other source regions through long- and short-range atmospheric transport. DDT was mainly used in the rural sites, whereas the sources of HCB, chlordane and endosulfan were mainly in the urban area. HCH was found almost equally in both urban and rural area. Soil concentrations of all detected OCPs, except HCHs, in and around Harbin were much lower than those in the southeast of China, which is expected since the use of these OCPs in the former was much lower than that in the latter, however higher HCH concentrations in and around Harbin than those found in most places of the Southeast China is not expected. It is suggested that high HCH concentration in soil of Northeast China was most likely due to long-range atmospheric transport （LRAT） from Southeast China and the cold condensation process.

关键词： organocholrine pesticides (OCPs) soil residue

Upgrading to urban water system 3.0 through sponge city construction

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Frontiers of Environmental Science & Engineering 2017年第4期11卷 3-10页

作者： nanqi ren Qian Wang Qiuru Wang Hong Huang Xiuheng Wang State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China Ministry of Environmental Protection of the People's Republic of China Beijing 100035 China

Facing the pressure of excessive water consumption, high pollution load and rainstorm waterlogging,linear and centralized urban water system, system 2.0, as well as traditional governance measures gradually exposed characters of water-sensitivity, vulnerability and unsustainability, subsequently resulting in a full-blown crisis of water shortage, water pollution and waterlogging. To systematically relieve such crisis, we established urban water system 3.0, in which decentralized sewerage systems, sponge infrastructures and ecological rivers play critical roles. Through unconventional water resource recycling, whole process control of pollutions and ecological restoration, system 3.0 with integrated management measures, is expected to fit for multiple purposes which involve environmental,ecological, economic and social benefits. With advantages of flexibility, resilience and sustainability, water system 3.0 will show an increasingly powerful vitality in the near future.

关键词： Water crisis Urban water system Sponge city Decentralized system Multi-purpose

Effects of various pretreatment methods on mixed microflora to enhance biohydrogen production from corn stover hydrolysate

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Journal of Environmental Sciences 2011年第12期23卷 1929-1936页

作者： Kun Zhang nanqi ren Changhong Guo Aijie Wang Guangli Cao State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province College of Life Science and Technology Harbin Normal University Harbin 150025 China

Five individual pretreatment methods, including three widely-used protocols （heat, acid and base） and two novel attempts （ultrasonic and ultraviolet）, were conducted in batch tests to compare their effects on mixed microflora to enhance hydrogen （H2） production from corn stover hydrolysate. Experimental results indicated that heat and base pretreatments significantly increased H2 yield with the values of 5.03 and 4.45 mmol H2/g sugar utilized, respectively, followed by acid pretreatment of 3.21 mmol H2/g sugar utilized. However, compared with the control （2.70 mmol H2/g sugar utilized）, ultrasonic and ultraviolet pretreatments caused indistinctive effects on H2 production with the values of 2.92 and 2.87 mmol H2/g sugar utilized, respectively. The changes of soluble metabolites composition caused by pretreatment were in accordance with H2-producing behavior. Concretely, more acetate accumulation and less ethanol production were found in pretreated processes, meaning that more reduced nicotinamide adenine dinucleotide （NADH） might be saved and flowed into H2-producing pathways. PCR-DGGE analysis indicated that the pretreatment led to the enrichment of some species, which appeared in large amounts and even dominated the microbial community. Most of the dominated species were affiliated to Enterobacter spp. and Escherichia spp. As another efflcient H2 producer, Clostridium bifermentan was only found in a large quantity after heat pretreatment. This strain might be mainly responsible for better performance of H2 production in this case.

关键词： biohydrogen pretreatment corn stover hydrolysate microbial community

Effect of seed sludge on characteristics and microbial community of aerobic granular sludge

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Journal of Environmental Sciences 2010年第9期22卷 1312-1318页

作者： Zhiwei Song Yuejun Pan Kun Zhang nanqi ren Aijie Wang School of Resource and Environmental Engineering Heilongjiang Institute of Science and Technology Harbin 150027 China School of Municipal and Environmental Engineering Harbin Institute of Technology Harbin 150001 China

Aerobic granular sludge was cultivated by using different kinds of seed sludge in sequencing batch airlift reactor. The influence of seed sludge on physical and chemical properties of granular sludge was studied; the microbial community structure was probed by using scanning electron microscope and polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）. The results showed that seed sludge played an important role on the formation of aerobic granules. Seed sludge taken from beer wastewater treatment plant （inoculum A） was more suitable for cultivating aerobic granules than that of sludge from municipal wastewater treatment plant （inoculurn B）. Cultivated with inoculum A, large amount of mature granules formed after 35 days operation, its SVI reached 32.75 mL/g, and SOUR of granular sludge was beyond 1.10 mg/（***）. By contrast, it needed 56 days obtaining mature granules using inoculum B. DGGE profiles indicated that the dominant microbial species in mature granules were 18 and 11 OTU when inoculum A and B were respectively employed as seed sludge. The sequencing results suggested that dominant species in mature granules cultivated by inoculum A were Paracoccus sp., Devosia hwasunensi, Pseudoxanthomonas sp., while the dominant species were Lactococcus raffinolactis and Pseudomonas sp. in granules developed from inoculum B.

关键词： aerobic granular sludge seed sludge microbial community PCR-DGGE

Levels and distribution of brominated flame retardants in the soil of Harbin in China

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Journal of Environmental Sciences 2009年第11期21卷 1541-1546页

作者： WANG Xu ren nanqi QI Hong MA Wanli LI Yifan Research Center for Persistent Toxic Substances State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China Science and Technology Braneh Environment Canada Toronto Ontario M3H 5T4 Canada

This study reports the presence of brominated flame retardants in the topsoil in and around Harbin, a city in northeastern China. Samples of soil were collected from 17 locations in 2006, and the levels of 9 polybrominated diphenylethers （PBDEs 17, 28, 47, 66, 99, 100, 153, 154, and 183） ranged from 2.45 to 55.9 pg/g dry weight （dw） with a mean of 26.3 pg/g dw. These levels are very low comparing with those for some cities in Europe and USA. BDE 209 and hexabromocyclododecane were the two dominant congeners, with mean concentrations of 520 pg/g dw and 1750 pg/g dw, respectively. The concentrations of the total nine PBDE congeners clearly decreased from urban areas to background, but the compositions of individual congeners differed. Proportions of heavier congeners decreased while those of lighter congeners increased, along urban-rural-background transect, providing evidence for an urban fractionation effect. Correlation analysis indicated similar sources for PBDEs, hexabromocyclododecane, and 1,2-bis（2,4,6-tribromophenoxy）-ethane from urban areas but pentabromoethylbenzene was probably present due to long-range atmospheric transport. Principal component analysis was used to determine the characteristics of the relationships among these brominated flame retardants in the field.

关键词： brominated flame retardants polybrominated diphenylether residue

Pollutants removal and simulation model of combined membrane process for wastewater treatment and reuse in submarine cabin for long voyage

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Journal of Environmental Sciences 2009年第11期21卷 1503-1512页

作者： CHEN Zhaobo ZHOU Aijuan ren nanqi TIAN Yu HU Dongxue State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China School of Materials Science & Chemical Engineering Harbin Engineering University Harbin 150001 China

A laboratory scale test was conducted in a combined membrane process （CMP） with a capacity of 2.91 m3/d for 240 d to treat the mixed wastewater of humidity condensate, hygiene wastewater and urine in submarine cabin during prolonged voyage. Removal performance of chemical oxygen demand （COD）, ammonia nitrogen （NH4^＋-N）, turbidity and anionic surfactants （LAS） was investigated under different conditions. It was observed that the effluent COD, NH4^＋-N, turbidity and LAS flocculated in ranges of 0.19-0.85 mg/L, 0.03-0.18 mg/L, 0.0-0.15 NTU and 0.0-0.05 mg/L, respectively in spite of considerable fluctuation in corresponding influent of 2120-5350 mg/L, 79.5-129.3 mg/L, 110-181.1NTU and 4.9-5.4 mg/L. The effluent quality of the CMP could meet the requirements of mechanical water and hygiene water according to the class I water quality standards in China （GB3838-2002）. The removal rates of COD, NH4^＋-N, turbidity and LAS removed in the MBR were more than 90%, which indicated that biodegradation is indispensable and plays a major role in the wastewater treatment and reuse. A model, built on the back propagation neural network （BPNN） theory, was developed for the simulation of CMP and produced high reliability. The average error of COD and NH4^＋-N was 5.14% and 6.20%, respectively, and the root mean squared error of turbidity and LAS was 2.76% and 1.41%, respectively. The results indicated that the model well fitted the laboratory data, and was able to simulate the removal of COD, NH4^＋-N, turbidity and LAS. It also suggested that the model proposed could reflect and manage the operation of CMP for the treatment of the mixed wastewaters in submarine.

关键词： combined membrane process wastewater treatment and reuse back propagation neural network

Effects of Iron on Hydrogen-producing Capacity, Hydrogenase and NADH-fd Reductase Activities of a Fermentative Hydrogen-producing Bacterial Strain B49

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High Technology Letters 2004年第4期10卷 69-74页

作者：王相晶 ren nanqi Xiang Wensheng Life Science College Northeast Agricultural University 150030 Harbin P.R.China College of Municipal & Environmental Engineering Harbin Institute of Technology 150090 Harbin P.R.China

Iron plays an important role in hydrogen production, cell growth, hydrogenase and NADH-fd reductase activities of hydrogen-producing bacterial strain B49 (AF481148 in EMBL). At the end of fermentation from 10 g/L glucose, for the culture containing 10 mg/L FeSO4*7H2O the cell growth in terms of optical density (OD) at 600nm was 1.13, the ratio of ethanol amount (mg/L) to acetate amount (mg/L) was 1.55, and the accumulated hydrogen volume was 1816.3 ml H2/L culture; whereas for the culture of 80 mg/L FeSO4*7H2O OD600nm was increased to 1.34, the accumulated hydrogen volume was increased to 2360.5 ml H2/L culture, and the ratio of ethanol amount (mg/L) to acetate amount (mg/L) decreased to 1.31. Moreover, the iron addition to the medium at different fermentation time could affect hydrogen-producing ability. However, the later the addition time of FeSO4*7H2O was postponed, the less the effect on hydrogen evolution was. In the course of fermentation, the specific activities of hydrogenase and NADH-fd reductase of hydrogen-producing bacterial strain B49 decreased with the consumption of iron.

关键词：氢化酶还原酶生物活性发酵工艺细菌 NADH-fd 能源清洁燃料生物燃料

One-pot hydrothermal fabrication of BiVO_(4)/Fe_(3)O_(4)/rGO composite photocatalyst for the simulated solar light-driven degradation of Rhodamine B

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Frontiers of Environmental Science & Engineering 2022年第3期16卷 105-120页

作者： Shuangyang Zhao Chengxin Chen Jie Ding Shanshan Yang Yani Zang nanqi ren State Key Laboratory of Urban Water Resource and Environment School of EnvironmentHarbin Institute of TechnologyHarbin 150090China

Fabrication of easily recyclable photocatalyst with excellent photocatalytic activity for degradation of organic pollutants in wastewater is highly desirable for practical *** this study,a novel ternary magnetic photocatalyst BiVO_(4)/Fe_(3)O_(4)/reduced graphene oxide(BiVO_(4)/Fe_(3)O_(4)/rGO)was synthesized via a facile hydrothermal *** BiVO_(4)/Fe_(3)O_(4) with 0.5 wt%of rGO(BiVO_(4)/Fe_(3)O_(4)/0.5%rGO)exhibited superior activity,degrading greater than 99%Rhodamine B(RhB)after 120 min solar light *** surface morphology and chemical composition of BiVO_(4)/Fe_(3)O_(4)/rGO were studied by scanning electron microscopy,X-ray diffraction,X-ray photoelectron spectroscopy,UV–visible diffuse reflectance spectroscopy,Fourier transform infrared spectroscopy,and Raman *** free radicals scavenging experiments demonstrated that hole(h^(+))and superoxide radical(O_(2)•^(−))were the dominant species for RhB degradation over BiVO_(4)/Fe_(3)O_(4)/rGO under solar *** reusability of this composite catalyst was also investigated after five successive runs under an external magnetic *** BiVO_(4)/Fe_(3)O_(4)/rGO composite was easily separated,and the recycled catalyst retained high photocatalytic *** study demonstrates that catalyst BiVO_(4)/Fe_(3)O_(4)/rGO possessed high dye removal efficiency in water treatment with excellent recyclability from water after *** current study provides a possibility for more practical and sustainable photocatalytic process.

关键词： Photocatalysis Ternary magnetic photocatalyst Visible-light-driven Free radicals trapping Reusability Recycling

Towards a better hydraulic cleaning strategy for ultrafiltration membrane fouling by humic acid: Effect of backwash water composition

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Journal of Environmental Sciences 2016年第5期28卷 177-186页

作者： Haiqing Chang Heng Liang Fangshu Qu Jun Ma nanqi ren Guibai Li State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology

As a routine measurement to alleviate membrane fouling, hydraulic cleaning is of great significance for the steady operation of ultrafiltration（UF） systems in water treatment processes. In this work, a comparative study was performed to investigate the effects of the composition of backwash water on the hydraulic cleaning performance of UF membranes fouled by humic acid（HA）. Various types of backwash water, including UF permeate, Milli-Q water, Na Cl solution, CaCl_2 solution and HA solution, were compared in terms of hydraulically irreversible fouling index, total surface tension and residual HA. The results indicated that Milli-Q water backwash was superior to UF permeate backwash in cleaning HA-fouled membranes, and the backwash water containing Na＋or HA outperformed Milli-Q water in alleviating HA fouling. On the contrary, the presence of Ca^（2＋） in backwash water significantly decreased the backwash efficiency. Moreover, Ca^（2＋） played an important role in foulant removal, and the residual HA content closely related to the residual Ca^（2＋） *** analysis suggested that the backwash process may involve fouling layer swelling, ion exchange, electric double layer release and competitive complexation. Ion exchange and competitive complexation played significant roles in the efficient hydraulic cleaning associated with Na＋and HA, respectively.

关键词： Ultrafiltration Membrane fouling Hydraulic cleaning Backwash water composition Humic acid