Effects of various pretreatment methods on mixed microflora to enhance biohydrogen production from corn stover hydrolysate

作     者：Kun Zhang Nanqi Ren Changhong Guo Aijie Wang Guangli Cao 

作者机构：State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province College of Life Science and Technology Harbin Normal University Harbin 150025 China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2011年第23卷第12期

页      面：1929-1936页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by the National Natural Science Foundation of China (No. 30870037, 30970552) the Funding of the National Creative Research Groups (No.50821002) the Aid Program for Science and Technology Innovative Research Team in Higher Educational Instituions of Heilongjiang Province (No. 2010TD10) the Harbin Normal University (No. KJTD2011-2) 

主　　题：biohydrogen pretreatment corn stover hydrolysate microbial community 

摘      要：Five individual pretreatment methods, including three widely-used protocols （heat, acid and base） and two novel attempts （ultrasonic and ultraviolet）, were conducted in batch tests to compare their effects on mixed microflora to enhance hydrogen （H2） production from corn stover hydrolysate. Experimental results indicated that heat and base pretreatments significantly increased H2 yield with the values of 5.03 and 4.45 mmol H2/g sugar utilized, respectively, followed by acid pretreatment of 3.21 mmol H2/g sugar utilized. However, compared with the control （2.70 mmol H2/g sugar utilized）, ultrasonic and ultraviolet pretreatments caused indistinctive effects on H2 production with the values of 2.92 and 2.87 mmol H2/g sugar utilized, respectively. The changes of soluble metabolites composition caused by pretreatment were in accordance with H2-producing behavior. Concretely, more acetate accumulation and less ethanol production were found in pretreated processes, meaning that more reduced nicotinamide adenine dinucleotide （NADH） might be saved and flowed into H2-producing pathways. PCR-DGGE analysis indicated that the pretreatment led to the enrichment of some species, which appeared in large amounts and even dominated the microbial community. Most of the dominated species were affiliated to Enterobacter spp. and Escherichia spp. As another efflcient H2 producer, Clostridium bifermentan was only found in a large quantity after heat pretreatment. This strain might be mainly responsible for better performance of H2 production in this case.