Effects of Iron on Hydrogen-producing Capacity, Hydrogenase and NADH-fd Reductase Activities of a Fermentative Hydrogen-producing Bacterial Strain B49

作     者：王相晶 Ren Nanqi Xiang Wensheng 

作者机构：Life Science College Northeast Agricultural University 150030 Harbin P.R.China College of Municipal & Environmental Engineering Harbin Institute of Technology 150090 Harbin P.R.China 

出 版 物：《High Technology Letters》 (高技术通讯（英文版）)

年 卷 期：2004年第10卷第4期

页      面：69-74页

核心收录：

学科分类：081702[工学-化学工艺] 080702[工学-热能工程] 08[工学] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 

基　　金：国家高技术研究发展计划(863计划) 国家重点基础研究发展计划(973计划) 

主　　题：氢化酶 还原酶 生物活性 发酵工艺 细菌 NADH-fd 能源 清洁燃料 生物燃料 

摘      要：Iron plays an important role in hydrogen production, cell growth, hydrogenase and NADH-fd reductase activities of hydrogen-producing bacterial strain B49 (AF481148 in EMBL). At the end of fermentation from 10 g/L glucose, for the culture containing 10 mg/L FeSO4*7H2O the cell growth in terms of optical density (OD) at 600nm was 1.13, the ratio of ethanol amount (mg/L) to acetate amount (mg/L) was 1.55, and the accumulated hydrogen volume was 1816.3 ml H2/L culture; whereas for the culture of 80 mg/L FeSO4*7H2O OD600nm was increased to 1.34, the accumulated hydrogen volume was increased to 2360.5 ml H2/L culture, and the ratio of ethanol amount (mg/L) to acetate amount (mg/L) decreased to 1.31. Moreover, the iron addition to the medium at different fermentation time could affect hydrogen-producing ability. However, the later the addition time of FeSO4*7H2O was postponed, the less the effect on hydrogen evolution was. In the course of fermentation, the specific activities of hydrogenase and NADH-fd reductase of hydrogen-producing bacterial strain B49 decreased with the consumption of iron.