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填土介质劈裂注浆加固机理试验及效果评价

科学技术与工程 2024年第22期24卷 9547-9557页

作者：李泽玮王丰年朱淳师永翔任宏玉牛建东山西省交通科技研发有限公司太原030032 河海大学地球科学与工程学院南京210000 中南大学土木工程学院长沙410075

为了探究填土介质劈裂注浆的浆液扩散规律和加固机理,设计了一套由试验台架系统、注浆系统和信息监测系统构成的三维模拟注浆试验系统,开展填土介质劈裂注浆试验研究。通过观察注浆加固后浆脉的性状和分布特征,分析浆液在填土介质中的... 详细信息

为了探究填土介质劈裂注浆的浆液扩散规律和加固机理,设计了一套由试验台架系统、注浆系统和信息监测系统构成的三维模拟注浆试验系统,开展填土介质劈裂注浆试验研究。通过观察注浆加固后浆脉的性状和分布特征,分析浆液在填土介质中的扩散规律和加固机理。对注浆前后的土体进行单轴压缩试验、轻型动力触探试验、渗透性试验以及室内土工试验,探讨了不同性状浆脉的分区特点与填土力学性能的关系,并对填土注浆后加固效果进行评价。最后,通过工程实例,验证了该模型试验和加固效果评价的合理性和可靠性。研究结果表明,填土介质劈裂注浆形成的浆脉主要由底部半球形浆泡以及呈板状的主干浆脉、网状分支浆脉和渗透充填浆脉4种性状浆脉组成,被注土体通过主干浆脉-分支浆脉-渗透充填浆脉的三级浆脉网络以及土体间的压密作用得到加强。注浆后,被注土体的单轴抗压强度平均提升186%,渗透系数平均减小47倍。平均黏聚力和内摩擦角分别提升45.3%和44.9%。此外,填土的密度、含水率等其他各项指标均有一定程度提高,动力触探试验结果反映的承载特性与浆脉的分布情况基本一致。

关键词：填土劈裂注浆浆脉加固机理效果评价

Microstructure and electrical properties of Sc_2O_3-doped CaCu_3Ti_4O_(12) ceramics

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Rare Metals 2015年第3期34卷 178-182页

作者： Dong Xu Ke Zhang Lei Jiao Kai He hong-Xing Xu Guo-Ping Zhao ren-hong yu School of Material Science and Engineering Jiangsu University State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of China Changzhou Engineering Research Institute of Jiangsu University Changzhou Ming Errui Ceramics Co. Ltd.

CaCu3Ti4O12 ceramics doped with different contents of Sc203 （mol%, x = 0, 0.05, 0.10, 0.15, and 0.20） were prepared by the traditional solid-state reaction method. Scanning electron microscope （SEM） and X-ray diffraction （XRD） were used in the microstructural studies of the specimen, and the electrical properties were inves- tigated. XRD results show that the Sc has no influence on the phase composition. The results from the dielectric measurements show that further increase of Sc doping could decrease the dielectric loss slightly. A high dielectric constant and low dielectric loss can be achieved when the doping concentration is 0.10 mol%.

关键词： CaCu3Ti4012 Rare earth Dielectricproperties Varistor Microstructure

Simultaneous Detection of 13 Key Bacterial Respiratory Pathogens by Combination of Multiplex PCR and Capillary Electrophoresis

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Biomedical and Environmental Sciences 2017年第8期30卷 549-561页

作者： JIANG Lu Xi ren hong yu ZHOU Hai Jian ZHAO Si hong HOU Bo Yan YAN Jian Ping QIN Tian CHEN yu Department of Respiratory Medicine Shengjing Hospital of China Medical University National Institute for Communicable Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and Prevention Department of Respiratory Medicine Zhejiang Provincial People's Hospital

Objective Lower respiratory tract infections continue to pose a significant threat to human health. It is important to accurately and rapidly detect respiratory bacteria. To compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction （PCR） and capillary electrophoresis （MPCE） assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catorrholis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Mycoplasma pneumoniae, Legionella spp., Bordetella pertussis, Mycobacterium tuberculosis complex, Corynebactefium diphthefiae, and Streptococcus pyogenes. Methods Three multiplex PCR reactions were built, and the products were analyzed by capillary electrophoresis using the high-throughput DNA analyzer. The specificity of the MPCE assay was examined and the detection limit was evaluated using DNA samples from each bacterial strain and the simulative samples of each strain. This assay was further evaluated using 152 clinical specimens and compared with real-time PCR reactions. For this assay, three nested-multiplex-PCRs were used to detect these clinical specimens. Results The detection limits of the MPCE assay for the 13 pathogens were very low and ranged from 10-7 to 10-2 ng/μL. Furthermore, analysis of the 252 clinical specimens yielded a specificity ranging from 96.5%-100.0%, and a sensitivity of 100.0% for the 13 pathogens. Conclusion This study revealed that the MPCE with high specificity and sensitivity. This assay survey of respiratory pathogens. assay is a rapid, reliable, and high-throughput method has great potential in the molecular epidemiological.

关键词： Respiratory pathogens Lower respiratory tract infections Multiplex PCR Capillary electrophoresis

Legionella dumoffii Tex-KL Mutated in an Operon Homologous to traC-traD is Defective in Epithelial Cell Invasion

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Biomedical and Environmental Sciences 2016年第6期29卷 424-434页

作者： QIN Tian Iida Ken-ichiro ren hong yu ZHOU Hai Jian Shin-ichi Yoshida State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention beijing 102206 China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003 Zhejiang China Departments of Bacteriology Faculty of Medical Sciences Kyushu University Higashi-ku Fukuoka 812-8582 Japan

Objective To understand the mechanism of invasion by Legionella dumoffii. Methods The L. dumoffii strain Tex-KL was mutated using the Tn903 derivative, Tn903 d IIlac Z. After screening 799 transposon insertion mutants, we isolated one defective mutant. We then constructed the gene-disrupted mutant, KL16, and studied its invasion of and intracellular growth in He La and A549 cells, and in A/J mice survival experiments. The structure of traC-traD operon was analyzed by RT-PCR. Results The transposon insertion was in a gene homologous to Salmonella typhi tra C, which is required for the assembly of F pilin into the mature F pilus structure and for conjugal DNA transmission. Results from RT-PCR suggested that the traC-traD region formed an operon. We found that when the tra C gene was disrupted, invasion and intracellular growth of L. dumoffii Tex-KL were impaired in human epithelial cells. When mice were infected by intranasal inoculation with a tra C deficient mutant, their survival significantly increased when compared to mice infected with the wild-type strain. Conclusion Our results indicated that the traC-traD operon is required for the invasion and intracellular growth abilities of L. dumoffii Tex-KL in epithelial cells.

关键词： L. dumoffii Tex-KL traC-traD operon Invasion ability Epithelial cells

Electrospinning-derived functional carbon-based materials for energy conversion and storage

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Chinese Chemical Letters 2024年第6期35卷 3-13页

作者： Xinyu ren hong Liu Jingang Wang Jiayuan yu Institute for Advanced Interdisciplinary Research(iAIR) School of Chemistry and Chemical EngineeringUniversity of JinanJi’nan 250022China State Key Laboratory of Crystal Materials Shandong UniversityJi’nan 250100China

The over-exploitation of fossil fuel energy has brought about serious environmental *** would be of great significance to construct efficient energy conversion and storage system to maximize utilize renewable energy,which contributes to reducing environmental *** the past few years,in terms of electrocatalysis and energy storage,carbon fiber materials show great advantages due to its outstanding electrical conductivity,good flexibility and mechanical *** a simple and low-cost technique,electrospinning can be employed to prepare various *** is noted that the functional fiber materials with different special structure and composition can be obtained for energy conversion and storage by combining electrospinning with other *** this paper,the structural design,controllable synthesis and multifunctional applications of electrospinning-derived functional carbon-based materials(EFCMs)is ***,we briefly introduce the history,basic principle and typical equipment of *** we discuss the strategies for preparing EFCMs with different structures and composition in *** addition,we show recently the application of advanced EFCMs in energy conversion and storage,such as nitrogen species reduction reaction,CO_(2) reduction reaction,oxygen reduction reaction,water-splitting,supercapacitors and ion *** the end,we propose some perspectives on the future development direction of EFCMs.

关键词： Electrospinning Carbon materials Functional fiber Energy conversion Energy storage Electrocatalysis

Phylogenetic Analysis of Legionella Strains and Identification of Serogroups by Lipopolysaccharide-and O-antigen-based PCR Assay

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Biomedical and Environmental Sciences 2021年第6期34卷 483-488页

作者： LI Yi Nan ren hong yu ZHAO Na WANG Yan Qing LI Dai QIN Tian State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102249China Nanchang Center for Disease Control and Prevention Nanchang 330100JiangxiChina Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310021JiangsuChina

Legionella,a genus of pathogenic Gram-negative bacteria,is widely present in natural water sources and artificial water systems.A total of 65 species and>70 serogroups of Legionella have been characterized[1].Lipopolysaccharide(LPS),the main component of the outer membrane of Legionella,is not only associated with toxicity,but also provides the basis for classification in serotyping[2].

关键词： Phylogenetic artificial outer

Isolation and Characterization of Vagococcus fluvialis from Bats

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Biomedical and Environmental Sciences 2021年第10期34卷 834-837页

作者： QIN Tian JIANG Lu Xi ren hong yu JIA Xue Yang NIE Xu Dong LI Yi Nan State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Department of Respiratory Medicine Zhejiang Provincial People's HospitalPeople's Hospital of Hangzhou Medical CollegeHangzhou 310000ZhejiangChina

The genus Vagococcus was first described by *** initially consisted of a single species,*** species was isolated from chicken feces and river water and first described by Hashimoto et al.[1,2].Teixeira et *** *** from... 详细信息

关键词： initially fluvialis coccus

Preparation and properties of poly(ethylene glycol)-bpoly( 3-acrylamidophenylboronic acid) glucose responsive particles and their hyaluronic acid-based microneedle patches

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第5期25卷 395-410页

作者： Yichuan hong Haojie yu Li WANG Xiang CHEN Di SHEN yu WANG Shuning ren yudi HUANG Jian YANG State Key Laboratory of Chemical Engineering College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310058China Zhejiang-Russia Joint Laboratory of Photo-Electro-Magnetic Functional Materials College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310058China

To overcome the pain and risk of hypoglycemia in insulin administration,glucose-responsive microneedles have been developed by researchers,which could release insulin according to the blood glucose *** designed a kind of particles by a reversible addition-fragmentation chain transfer(RAFT)method containing a phenylboronic acid group as the sensor of glucose and carrier of ***(ethylene glycol)(PEG)-2-(dodecylthio(thiocarbonyl)thio)-2-methylpropionic acid(DDMAT)was synthesized as a macromolecular RAFT agent,which was then reacted with 3-acrylamidophenylboronic acid(AAPBA)to synthesize the block copolymer ***-responsive particles loaded with insulin were prepared by self-assembly based on hydrophilic-hydrophobic *** patches loaded with glucose-responsive particles were prepared using hyaluronic acid as the *** insulin release behavior of the particles in glucose solutions of 0,100,and 400 mg/dL showed significant glucose responsiveness and good *** results of blood glucose control experiments in rats indicate that a single microneedle patch can effectively maintain normal blood glucose for over 7 h.

关键词： Diabetes Hyaluronic acid Phenylboronic acid(PBA) Glucose responsive hydrogel Microneedles

IFN-a production by human mononuclear cells infected with varicella-zoster virus through TLR9-dependent and-independent pathways

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Cellular & Molecular Immunology 2011年第2期8卷 181-188页

作者： hong-ren yu Hsin-Chun Huang Ho-Chang Kuo Jiunn-Ming Sheen Chia-Yo Ou Te-Yao Hsu Kuender D Yang Department of Pediatrics Chang Gung Memorial Hospital-Kaohsiung Medical CenterGraduate Institute of Clinical Medical ScienceChang Gung University College of MedicineTaiwan Department of Obstetrics Chang Gung Memorial Hospital-Kaohsiung Medical CenterGraduate Institute of Clinical Medical ScienceChang Gung University College of MedicineTaiwan

Understanding the defense mechanisms of the host of an organism is important for infection *** previous studies,we demonstrated that interferon-a(IFN-a),but not IL-12,was produced by human peripheral blood mononuclear cells infected with varicella-zoster virus(VZV).Here,we investigated what kind of cell(s)and which signal molecule(s)are involved in IFN-a *** cell isolation and ELISA,we found that plasmacytoid dendritic cells(pDCs)were responsible for IFN-a production during VZV *** also found that Toll-like receptor 9(TLR9)was involved in VZV-induced IFN-a production because inhibitory CpG oligodeoxynucleotide inhibited IFN-a ***-inactivated VZV-induced IFN-a production was lower than that of active VZV,indicating another TLR9-independent *** studies demonstrated that double-stranded RNA-dependent protein kinase,but not DNA-dependent protein kinase was involved in VZV-induced IFN-a ***,these results suggest that pDCs play an important role in IFN-a production during VZV infection through TLR9-dependent and-independent pathways.

关键词： IFN-a mononuclear cells plasmacytoid dendritic cell TLR9 varicella-zoster virus