检索结果-南通市图书馆

On-chip detection of a single nucleotide polymorphism without polymerase amplification

Nano research 2014年第9期7卷 1302-1310页

作者： Jinhee Han Matthew Tan Lakshmana Sudheendra robert H. Weiss Ian M. kennedy Department of Mechanical and Aerospace Engineering University of California Davis California 95616 USA Division of Nephrology Department of Internal Medicine University of California Davis California 95616 USA Medical Service Sacramento VA Medical Center Sacramento California 95655 USA

A nanoparticle-assembled photonic crystal （PC） array was used to detect single nucleotide polymorphism （SNP）. The assay platform with PC nanostructure enhanced the fluorescent signal from nanoparticle-hybridized DNA complexes due to phase matching of excitation and emission. Nanoparticles coupled with probe DNA were trapped into nanowells in an array by using an electrophoretic particle entrapment system. The PC/DNA assay platform was able to identify a 1 base pair （bp） difference in synthesized nucleotide sequences that mimicked the mutation seen in a feline model of human autosomal dominant polycystic kidney disease （PKD） with a sensitivity of 0.9 fg/mL （50 aM）-sensitivity, which corresponds to 30 oligos/array. The reliability of the PC/DNA assay platform to detect SNP in a real sample was demonstrated by using genomic DNA （gDNA） extracted from the urine and blood of two PKD-wild type and three PKD positive cats. The standard curves for PKD positive （PKD＋） and negative （PKD-） DNA were created using two feline-urine samples. An additional three urine samples were analyzed in a similar fashion and showed satisfactory agreement with the standard curve, confirming the presence of the mutation in affected urine. The limit of detection （LOD） was 0.005 ng/mL which corresponds to 6 fg per array for gDNA in urine and blood. The PC system demonstrated the ability to detect a number of genome equivalents for the PKD SNP that was very similar to the results reported with real time polymerase chain reaction （PCr）. The favorable comparison with quantitative PCr suggests that the PC technology may find application well beyond the detection of the PKD SNP, into areas where a simple, cheap and portable nucleic acid analvsis is desirable.

关键词： photonic crystal array single nucleotidepolymorphisms DNA polycystic kidney disease real time polymerasechain reaction (PCr)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Numerical aerodynamic analysis of bluff bodies at a high reynolds number with three-dimensional CFD modeling

引用

Science China(Physics,Mechanics & Astronomy) 2013年第2期56卷 277-289页

作者： BAI YuGuang YANG Kai SUN DongKe ZHANG YuGuang kennedy David WILLIAMS Fred GAO XiaoWei State Key Laboratory of Structural Analysis for Industrial Equipment Faculty of Vehicle Engineering and MechanicsDalian University of Technology Cardiff School of Engineering Cardiff UniversityCardiff CF24 3AAWalesUK

This paper focuses on numerical simulations of bluff body aerodynamics with three-dimensional CFD(computational fluid dynamics) modeling,where a computational scheme for fluid-structure interactions is *** choice of an appropriate turbulence model for the computational modeling of bluff body aerodynamics using both two-dimensional and three-dimensional CFD numerical simulations is also *** efficient mesh control method which employs the mesh deformation technique is proposed to achieve better simulation *** long-span deck sections are chosen as examples which were stationary and pitching at a high reynolds *** the proposed CFD method and turbulence models,the force coefficients and flutter derivatives thus obtained are compared with the experimental measurement results and computed values completely from commercial ***,a discussion on the effects of oscillation amplitude on the flutter instability of a bluff body is carried out with extended numerical *** numerical analysis results demonstrate that the proposed three-dimensional CFD method,with proper turbulence modeling,has good accuracy and significant benefits for aerodynamic analysis and computational FSI studies of bluff bodies.

关键词： bluff body aerodynamic analysis fluid-structure interaction three-dimensional CFD modeling flutter

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

小孔径泡沫铝的制备及压缩性能研究

引用

稀有金属材料与工程 2009年第A3期38卷 254-259页

作者：左孝青 kennedy A r 王永胜毕于顺陆建生周芸昆明理工大学云南昆明650093 Advanced Materials Group School of M3University of NottinghamUniversity Park

在常规熔体发泡法基础上,采用添加0.5%Mg(质量分数,下同)以降低表面张力;发泡剂400℃,6h+500℃,1h氧化预处理以协调发泡剂分散均匀性与发泡过程关系;发泡搅拌60s以破碎初始气泡等措施,成功制备出了平均孔径1.3mm、孔隙率70.5%、结构均... 详细信息

在常规熔体发泡法基础上,采用添加0.5%Mg(质量分数,下同)以降低表面张力;发泡剂400℃,6h+500℃,1h氧化预处理以协调发泡剂分散均匀性与发泡过程关系;发泡搅拌60s以破碎初始气泡等措施,成功制备出了平均孔径1.3mm、孔隙率70.5%、结构均匀的小孔径泡沫铝。泡沫铝及Al-9Si泡沫的压缩性能分析表明,随平均孔径减小,泡沫铝的屈服强度、致密化应变和能量吸收能力均明显提高,泡沫铝压缩性能随孔径减小而提高,与泡沫铝的孔结构因素及孔结构均匀性有关。

关键词：泡沫铝小孔径制备压缩性能

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Echoes of the past:prevalence and correlates of PTSD among formerly abducted youths in northern Uganda:findings from the WAYS study

引用

General Psychiatry 2022年第5期35卷 309-318页

作者： kennedy Amone-P'Olak Bernard Omech Adrian Ivan Kakinda Henry Kibedi Psychology Kyambogo UniversityKampalaUganda Department of Public Health Lira UniversityLiraUganda

Background Post-traumatic stress disorder(PTSD)has considerable and persistent effects on survivors of war,particularly in postconflict ***,evidence on what keeps survivors on the path of PTSD remains *** The current study aimed to assess the prevalence and correlates of PTSD symptoms among the war-affected population in northern *** Data from 476 war-affected youths(aged 20–27 years)who had participated in a longitudinal cohort study were analysed to describe the enduring associations of background and postwar environmental risk factors with PTSD *** Impact of Event Scale-revised was used to evaluate symptoms of *** statistics were used to compute background and postwar environmental *** logistic regression analyses were fitted to assess the magnitude of the effects of the correlates on PTSD *** Background and postwar environmental factors(eg,sexual abuse and injury in captivity)were significantly associated with PTSD *** environmental factors associated with PTSD symptoms included postwar hardships,stigma/discrimination,chronic illness,community relations,family acceptance and general functioning,among *** odds ratios(Ors)for postwar hardships were 2.41(95%confidence interval(CI):1.63 to 3.56)and 2.90(95%CI:2.03 to 4.14)for high and severe PTSD,*** stigma/discrimination,compared with higher scores,the Ors were 3.38(95%CI:2.22 to 5.17)and 4.12(95%CI:2.69 to 6.30)for high and severe PTSD symptoms,*** Background and postwar environmental stressors exacerbate the severity of PTSD symptoms in survivors of war and should form the basis for interventions to alleviate the toxic effects of war on survivors.

关键词： prevalence finding among

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Modelling the biological performance of a side-stream membrane bioreactor using ASM1

引用

Journal of Environmental Sciences 2004年第6期16卷 1020-1023页

作者： TIANKe-jun LIUXin-ai JIANGTao M.D.kennedy J.C.Schippers P.A.Vanrolleghem MinistryofWaterresourcesofPrC Beijing100053China UNESCO-IHEInstituteforWaterEducation POBox30152601DADelftTheNetherlands BIOMATH GhentUniversityCoupureLinks653B-9000GentBelgium

Membrane bioreactors(MBrs) are attracting global interest but the mathematical modeling of the biological performance of MBrs remains very limited. This study focuses on the modelling of a side-stream MBr system using Activated Sludge Model No.1(ASM1), and comparing the results with the modelling of traditional activated sludge processes. ASM1 parameters relevant for the long-term biological behaviour in MBr systems were calibrated(i.e. Y H=0.72gCOD/gCOD, Y A=0.25gCOD/gN, b H=0.25 d -1, b A=0.080 d -1 and f P=0.06), and generally agreed with the parameters in traditional activated sludge processes, with the exception that a higher autotrophic biomass decay rate was observed in the MBr. A sensitivity analysis for steady state operation and DO dynamics suggested that the biological performance of the MBr system(the sludge concentration, effluent quality and the DO dynamics) are very sensitive to the parameters(i.e. Y H, Y A, b H, b A, μ maxH and μ maxA), and influent wastewater components(X I, S S, X S, S NH).

关键词： membrane bioreactor modelling parameter estimation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A thin Si nanowire network anode for high volumetric capacity and long-life lithium-ion batteries

引用

Journal of Energy Chemistry 2023年第6期81卷 20-27,I0002页

作者： Ibrahim Saana Amiinu Sumair Imtiaz Hugh Geaney Tadhg kennedy Nilotpal Kapuria Shalini Singh Kevin M ryan Bernal Institute University of LimerickLimerick V94 T9PXIreland Department of Chemical Sciences University of LimerickLimerick V94 T9PXIreland

Silicon nanowires(Si NWs)have been widely researched as the best alternative to graphite anodes for the next-generation of high-performance lithium-ion batteries(LIBs)owing to their high capacity and low discharge ***,growing binder-free Si NW anodes with adequate mass loading and stable capacity is severely limited by the low surface area of planar current collectors(CCs),and is particularly challenging to achieve on standard pure-Cu substrates due to the ubiquitous formation of Li+inactive silicide ***,the growth of densely-interwoven In-seeded Si NWs is facilitated by a thin-film of copper-silicide(CS)network in situ grown on a Cu-foil,allowing for a thin active NW layer(real loading(≈1.04 mg/cm^(2))binder-free electrode *** electrode exhibits an average Coulombic efficiency(CE)of>99.6%and stable performance for>900 cycles with≈88.7%capacity *** significantly,it delivers a volumetric capacity of≈1086.1 m A h/cm^(3)at *** full-cell versus lithium manganese oxide(LMO)cathode delivers a capacity of≈1177.1 m A h/g at 1C with a stable rate *** electrode architecture represents significant advances toward the development of binder-free Si NW electrodes for LIB application.

关键词： Si NW anode Silicidation LMO cathode Amorphous ligaments Volumetric capacity Lithium-ion batteries

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Global Boundary Stratotype Section and Point （GSSP） for the base of the Cenomanian Stage, Mont risou, Hautes-Alpes, France

引用

Episodes 2004年第1期27卷 21-32页

作者： W.J.kennedy A.S.Gale J.A.lees M.Caron OxfordUniversityMuseumofNaturalHistory ParksRoadOxfordOXI3PWU.K. DepartmentofPalaeontology TheNaturalHistoryMuseumCromwellRoadLondonSW75BPU.K.andSchoolofEarthandEnvironmentalSciencesUniversityofGreenwichChathamMaritimeCampusChathamKentME44AWU.K. researchSchoolofGeologicalandGeophysicalSciences BirkbeckCollegeandUniversityCollegeLondonGowerStreetLondonWCIE6BTU.K. InstitutdeGeologie UniversitedeFribourgCH-1700FribourgSwitzerland.

Following the unanimous recommendation of the International Commission on Stratigraphy, the Global boundary Stratotype Section and Point (GSSP) for the base of the Cenomanian Stage is defined at a level 36 metres below the top of the Marnes Bleues Formation, a level that corresponds to the the first appearance of the planktonic foraminiferan rotalipora globotruncanoides Sigal, 1948, on the south side of Mont risou, east of rosans, Haute-Alpes, France, where it can be placed in the context of a series of secondary marker levels based on nannofossils, planktonic foraminifera, ammonites,and an ornate δ^13C curve.

关键词：地层学地层分界线层型地质构造

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Diagnostic and therapeutic potentials of microrNAs in cholangiopathies

引用

Liver research 2017年第1期1卷 34-41页

作者： Lindsey kennedy Heather Francis Fanyin Meng Shannon Glaser Gianfranco Alpini research Central Texas Veterans Health Care SystemTempleTXUSA Department of Medicine Texas A&M Health Science CenterCollege of MedicineTempleTXUSA Baylor Scott&White Digestive Disease research Center Baylor Scott&White HealthTempleTXUSA

Cholangiopathies are a group of rare,devastating diseases that arise from damaged cholangiocytes,the cells that line the intra-and extra-hepatic bile ducts of the biliary *** result in significant morbidity and mortality and are a major cause of liver transplantation.A better understanding of the underlying pathogenesis that influences cholangiocyte dysregulation and cholangiopathy progression is necessary,considering the dismal prognosis associated with these *** are a class of small,non-coding rNAs that regulate post-transcriptional mrNA expression of specific *** role of microrNAs has expanded to include the initiation and development of many diseases,including *** microrNA regulation of cholangiopathies may provide diagnostic and therapeutic benefit for these *** this review,the authors primarily focus on studies published within the last five years that help determine the diagnostic and therapeutic potential of microrNAs in cholangiopathies.

关键词： microrNAs Cholangiopathies Diagnosis Therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular crosstalk during cholestatic liver injury

引用

Liver research 2017年第1期1卷 26-33页

作者： Joanne Thomson Laura Hargrove Lindsey kennedy Jennifer Demieville Heather Francis research Central Texas Veterans Healthcare SystemTXUSA Digestive Disease research Center Baylor Scott&White HealthTXUSA Medicine Texas A&M Health Science CenterTempleTXUSA

The functions of the liver are very *** detoxifying blood to storing glucose in the form of glycogen and producing bile to facilitate fat digestion,the liver is a very active and important *** liver is comprised of many varied cell types whose functions are equally *** line the biliary tree and aid in transporting and adjusting the composition of bile as it travels to the *** stellate cells and portal fibroblasts are located in different areas within the liver architecture,but both contribute to the development of fibrosis upon activation after liver *** cells,including those that constitute the peribiliary vascular plexus,are involved in functions other than blood delivery to and from the liver,such as supporting the growth of the biliary tree during *** cells are normally found in healthy livers but in very low ***,after injury,mast cell numbers greatly increase as they infiltrate and release factors that exacerbate the fibrotic *** not an all-inclusive list,these cells have individual roles within the liver,but they are also able to communicate with each other by cellular *** this review,we examine some of these pathways that can lead to an increase in the homeostatic dysfunction seen in liver injury.

关键词： Mast cells Paracrine Cholangiocytes Hepatic stellate cells Crosstalk

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Assessment of the uncertainties in temperature change in China during the last century

引用

Chinese Science Bulletin 2010年第19期55卷 1974-1982页

作者： LI QingXiang DONG WenJie LI Wei GAO Xiaorong JONES P kennedy J PArKEr D National Meteorological Information Center Beijing 100081 China Beijing Normal University Laboratory of Surface Process and Resources Economies Beijing 100875 China National Meteorological Center Beijing 100081 China Climatic research Unit School of Environmental Sciences University of East Anglia Norwich NR4 7TJ UK Met Office Hadley Centre Exeter EX1 3PB UK

We have used the China Homogenized Historic Temperature dataset and some long-term station series of the neighbor countries from CrUTEM3,a 5°×5° gridded dataset of monthly mean temperature since 1900,to provide a 107-year record of surface temperature trends and *** derived a comprehensive set of uncertainty estimates to accompany the data:measurement and sampling errors,uncertainties in temperature bias estimates,and uncertainties arising from limited observational coverage on large-scale averages have all been *** reanalysed the temperature changes during the period of *** best estimates of trends for 1900-2006 with uncertainties at 95% confidence range are about 0.09±0.017°C/decade for the year as a whole,and 0.14±0.021°C/decade,0.11±0.021°C/decade,0.04±0.017°C/decade,and 0.07±0.017°C/decade for winter,spring,summer and autumn *** 1954-2006,the trends for annual,winter,spring,summer and autumn are:0.26±0.032°C/decade,0.35±0.046°C/decade,0.25±0.051°C/decade,0.16±0.037°C/decade and 0.22±0.055°C/*** saw the most significant warming trend in both 1900-2006 and 1954-2006,while during the most recent period(the satellite era,1979-2006),all the seasons show similar warming trends:0.45±0.13°C/decade,0.51±0.11°C/decade,0.52±0.16°C/decade,0.37±0.10°C/decade and 0.50±0.16°C/decade for annual,winter,spring,summer and *** arising from urbanization have been evaluated as less than 5% of the total warming trend for 1951-2001,so this bias was not removed.

关键词：不确定性温度变化中国历史评估变暖趋势偏差估计月平均温度抽样误差

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：