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Lactate dehydrogenase:An important molecule involved in acetamizuril action against Eimeria tenella

Journal of Integrative Agriculture 2020年第5期19卷 1332-1339页

作者： LIU Li-li fei Chen-zhong DONG Hui zhang Ke-yu Fu Jian-jun LI Tao XUE fei-qun Key Laboratory of Animal Parasitology Ministry of Agriculture/Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghai 200241P.R.China East China University of Science and Technology Shanghai 200237P.R.China

Lactate dehydrogenase(LDH),a vital enzyme in anaerobic glycolysis,is closely associated with the survival of *** studies of some parasites have shown that LDH exhibits unique physicochemical properties and molecular structures and may be an ideal potential target for diagnosis and drug *** this study,we aimed to investigate the effects of acetamizuril,a novel anticoccidial compound,on LDH in the second-generation merozoites of Eimeria tenella(mz-LDH).Quantitative real-time PCR,Western blot,immunofluorescence and enzyme activity assays were each applied to detect the changes of *** results indicated that the mRNA and protein levels of mz-LDH were reduced upon acetamizuril *** of mz-LDH demonstrated that considerable amount of mz-LDH was distributed around or in the nuclei of second-generation merozoites within the untreated group;in contrast,the acetamizuril-treated group had very low level of ***,LDH enzyme activity assay suggested that a decreased LDH enzyme activity in both cytoplasm and nucleus of merozoites in the acetamizuril-treated ***,the induced apoptosis in second-generation merozoites by the acetamizuril was evaluated by detecting caspase 3 activity,and acetamizuril was found to significantly increase caspase 3 *** above findings show that LDH may play an important role in the mediating the activity of acetamizuril against coccidiosis,and further investigation into this aspect might contribute to new light on the pathogenesis of *** during its interaction with acetamizuril.

关键词： acetamizuril lactate dehydrogenase E.tenella second-generation merozoites

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A reduced-order extrapolation algorithm based on CNLSMFE formulation and POD technique for two-dimensional Sobolev equations

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Applied Mathematics(A Journal of Chinese Universities) 2014年第2期29卷 171-182页

作者： LIU qun TENG fei LUO Zhen-dong School of Mathematics and Computer Science Guizhou Normal University School of Mathematics Sciences Kaili college School of Mathematics and Physics North China Electric Power University

A reduced-order extrapolation algorithm based on Crank-Nicolson least-squares mixed finite element （CNLSMFE） formulation and proper orthogonal decomposition （POD） technique for two-dimensional （2D） Sobolev equations is established. The error estimates of the reduced-order CNLSMFE solutions and the implementation for the reduced-order extrapolation algorithm are provided. A numerical example is used to show that the results of numerical computations are consistent with theoretical conclusions. Moreover, it is shown that the reduced-order extrapolation algorithm is feasible and efficient for seeking numerical solutions to 2D Sobolev equations.

关键词： Reduced-order extrapolation aigorithm Crank-Nicolson least*squares mixed finite element for-mulation proper orthogonal decomposition technique Sobolev equations.

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日粮中添加枯草芽孢杆菌对高氧气调包装猪肉蛋白质和脂质氧化的影响

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食品科学 2025年第1期46卷 142-148页

作者：张悦于江刘飞张玉玉张一敏毛衍伟山东农业大学食品科学与工程学院山东泰安271018 山东省农业科学院畜牧兽医研究所农业农村部畜禽生物组学重点实验室山东济南250100

为探究日粮添加枯草芽孢杆菌对猪肉抗氧化能力以及高氧气调包装贮藏期间猪肉肉色、蛋白质氧化、脂质氧化和感官品质的影响,选取对照组(只饲喂基础日粮)和处理组(基础日粮添加枯草芽孢杆菌)的三元杂交(杜洛克×长白×大约克)猪各6只,屠... 详细信息

为探究日粮添加枯草芽孢杆菌对猪肉抗氧化能力以及高氧气调包装贮藏期间猪肉肉色、蛋白质氧化、脂质氧化和感官品质的影响,选取对照组(只饲喂基础日粮)和处理组(基础日粮添加枯草芽孢杆菌)的三元杂交(杜洛克×长白×大约克)猪各6只,屠宰后取背最长肌测定抗氧化酶活性,其余背最长肌样品进行高氧气调包装,4℃冷库贮藏,分别在0、5、10、15、20?d测定肉色、丙二醛(malondialdehyde,MDA)、羰基和巯基含量,并进行感官评价。结果表明:与对照组相比,处理组能够显著提高超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性(P<0.05),增强猪肉的抗氧化能力;日粮中添加枯草芽孢杆菌可以显著改善高氧气调包装猪肉的肉色(P<0.05);在低温贮藏期间,处理组能够显著降低MDA含量(P<0.05),抑制高氧气调包装猪肉的脂肪氧化;日粮中添加枯草芽孢杆菌能够显著抑制高氧气调包装猪肉的蛋白质氧化、羰基的生成和巯基损失(P<0.05),同时显著提高猪肉打开包装前的颜色和打开包装后的总体喜爱度评分(P<0.05)。综上,在日粮中添加枯草芽孢杆菌可提高猪肉的抗氧化能力、改善猪肉在高氧气调包装中的肉色、抑制蛋白质和脂质氧化,提高猪肉的食用品质。

关键词：枯草芽孢杆菌猪肉抗氧化能力脂质氧化蛋白质氧化

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Existence and Regularity of Solution to the Generalized Tricomi Equation with a Singular Initial Datum at a Point

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Acta Mathematica Sinica,English Series 2012年第6期28卷 1135-1154页

作者： Kang qun zhang Department of Mathematics&IMS Nanjing University

In this paper, we study the existence and regularity of a solution to the initial datum problem of a semilinear generalized Tricomi equation in mixed-type initial datum on the degenerate plane is smooth away from the origin, domain. We suppose that an and has a conormal singularity at this point, then we show that in some mixed-type domain, the solution exists and is conormal with respect to the characteristic conic surface which is issued from the origin and has a cusp singularity.

关键词： Generalized Tricomi equation confluent hypergeometric function existence conormalregularity cusp singularity

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Investigation of Formation Process of the Chrome-free Passivation Film of Electrodeposited Zinc

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Chinese Journal of Aeronautics 2007年第2期20卷 129-133页

作者： ZHU Li-qun YANG fei HUANG Hui-jie School of Materials Science and Engineering Beijing University of Aeronautics and Astronautics Beijing 100083 China

The feasibility was investigated to substitute chrome-free passivation treatment of electrodeposited zinc in a titanium bath for chromate passivation treatment. The formation mechanism of the chrome-free passivation film was further analyzed. The surface mor- phologies and the elemental compositions of the treated samples with varied immersion times were observed by scanning electron mi- croscopy （SEM） and determined by energy dispersion spectrometry （EDS）, respectively. The electrode potential of the sample surface was recorded in the film formation process. The changes of the electrode potential are in accordance with that of SEM and EDS of the sample surface. The results of X-ray photoelectron spectroscopy （XPS） show the chrome-free passivation film composed ofZnO, SiO2, TiO2, Zn4Si207（OH）2, and SrF2. The anode zinc dissolution and the local pH value increase due to the cathode hydrogen ion reduction process result in the formation of the chrome-free passivation film. The macro-images of the chrome-free passivation films formed on electrodeposited zinc show that the color of the film changes from blue to iridescence with the increase of the immersion times.

关键词： electrodeposited zinc chrome-free pasivation film film formation process

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Expression of Lung Surfactant Proteins SP-B and SP-C and Their Regulatory Factors in Fetal Lung of GDM Rats

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Current Medical Science 2018年第5期38卷 847-852页

作者： Qing-miao zhang Wei-xiang OUYANG Xin-qun CHAI fei-tao DENG Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Hepatobiliary Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

This study investigated the expression of lung surfactant proteins (SP-B and SP-C),and regulatory factors [forkhead box A2(FOXA2)and nitrolyogenic FOXA2 (N-FOXA2)]in the fetal lung of rats with gestational diabetes mellitus (GDM)in order to study the mechanism of pulmonary *** rat GDM model was established by using streptozotocin intraperitoneally in the first stage of *** were 10 rats in the GDM group,and 10 healthy rats in normal control group without any *** lungs of two groups were taken at day 21 of *** glucose levels of maternal rats and fetal rats were measured by Roche blood glucose *** histological changes in the fetal lung were observed under the light microscope in both *** SP-B,SP-C and FOXA2were determined in the fetal lung of two groups immunohistochemically. The expression levels of SP-B,SP-C,total FOXA2,FOXA2 in nucleus (n-FOXA2), N-FOXA2 proteins were detected by Western blotting,and the relative expression levels of SP-B,SP-C,FOXA2 mRNA in the fetal lung of two groups were detected by *** results showed that blood glucose levels of maternal rats and fetal rats in GDM group were higher than those in control *** light microscope revealed fetal lung development retardation in GDM *** expression of SP-B and SP-C in GDM group was significantly reduced as compared with control group (P0.05).It was concluded that GDM can cause fetal lung development and maturation disorders,and FOXA2 in fetal lung tissue decreases while nitrocellulose FOXA2 increases.

关键词： nitrolyogenic FOXA2 fetal lung development gestational diabetes mellitus

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A Note on Initial Value Problem for the Generalized Tricomi Equation in a Mixed-type Domain

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Acta Mathematica Sinica,English Series 2013年第8期29卷 1581-1596页

作者： Kang qun zhang Department of Mathematics and Physics Nanjing Institute of Technology

In this paper, we study the well-posedness of initial value problem for n-dimensional gener-alized Tricomi equation in the mixed-type domain {（t,x）：t∈[1,＋∞）,x∈Rn} with the initial data given on the line t=1 in Hadamard＇s sense. By taking partial Fourier transformation, we obtain the explicit expression of the solution in terms of two integral operators and further establish the global estimate of such a solution for a class of initial data and source term. Finally, we establish the global solution in time direction for a semilinear problem used the estimate.

关键词： Generalized Tricomi equation semilinear initial value problem mixed-type domain

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Existence of a-Cores for Games Without Compact Assumptions

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Journal of the Operations Research Society of China 2024年第2期12卷 520-527页

作者： Hai-qun zhang School of International Economics and Trade Shanghai Lixin University of Accounting and Fin Shanghai201209China

Following the representation of Kajii(J Econ Theory 56:194-205,1992),we provide some existence theorems ofα-cores for games without ordered preferences and compact *** applications,we obtain some existence results of... 详细信息

关键词： α-Core Existence Preference Noncompactness

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Th17 cells and IL-17 are involved in the disruption of vulnerable plaques triggered by short-term combination stimulation in apolipoprotein E-knockout mice

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Cellular & Molecular Immunology 2013年第4期10卷 338-348页

作者： Tian Ma Qi Gao Faliang Zhu Chun Guo qun Wang fei Gao Lining zhang Department of Immunology Shandong University School of Medicine and Jinan China Key Laboratory of Cardiovascular Remodeling and Function Research Qilu Hospital Shandong University .linan China

Considerable evidence indicates that type 1 T helper （Th 1）- and Th 17-mediated immune responses promote the formation of atherosclerotic plaques while that CD4＋CD25＋Foxp3＋ regulatory T cells （Tregs） have a protective effect. However, the functions of diverse CD4＋ lymphocyte subsets in plaque rupture remain poorly understood because of a shortage of satisfactory plaque rupture models. Here, we established a murine model of atherosclerotic plaque rupture using a high-fat diet and collar placement on the carotid artery, and triggered plaque rupture by short-term stimulation with a combination of lipopolysaccharide, phenylephrine injection and cold in apolipoprotein E-knockout （ApoE-/-） mice. We investigated the associations between Thl cells, Th17 cells and Tregs and plaque rupture by PCR, flow cytometry, ELISA and immunohistochemistry. In total, 75% （18/24） of vulnerable plaques, but no stable plaques, showed rupture characteristics. The proportion of Th17 cells was increased among splenocytes after treatment, but the changes in the levels of Thl ceils and Tregs were not related to rupture. Furthermore, the treatment resulted in high levels of interleukin- 17 （IL-17） in the serum and in the region of plaque rupture, in vitro, IL-17 increased the level of apoptosis, a major factor associated with plaque rupture, in cultured murine vascular smooth muscle cells. Th17 cells and IL-17 may be involved in the disruption of vulnerable plaques triggered by short-term stimulation with lipopolysaccharide, phenylephrine injection and cold in ApoE-/-mice.

关键词： atherosclerosis mice plaque rupture Th1 Th17

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Dynamic Accuracy Design Method of Ultra-precision Machine Tool

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Chinese Journal of Mechanical Engineering 2018年第1期31卷 167-175页

作者： Guo-Da Chen Ya-Zhou Sun fei-Hu zhang Li-Hua Lu Wan-qun Chen Nan Yu Center for Precision Engineering Harbin Institute of Technology Key Laboratory of E&M Ministry of Education & Zhejiang Province Zhejiang University of Technology Precision Engineering Institute Cranfield University

Ultra-precision machine tool is the most important physical tool to machining the workpiece with the frequency domain error requirement, in the design process of which the dynamic accuracy design(DAD) is indispensable and the related research is rarely available. In light of above reasons, a DAD method of ultra-precision machine tool is proposed in this paper, which is based on the frequency domain error *** basic procedure and enabling knowledge of the DAD method is introduced. The application case of DAD method in the ultra-precision flycutting machine tool for KDP crystal machining is described to show the procedure detailedly. In this case, the KDP workpiece surface has the requirements in four different spatial frequency bands, and the emphasis for this study is put on the middle-frequency band with the PSD specifications. The results of the application case basically show the feasibility of the proposed DAD method. The DAD method of ultra-precision machine tool can effectively minimize the technical risk and improve the machining reliability of the designed machine tool. This paper will play an important role in the design and manufacture of new ultra-precision machine tool.

关键词： Dynamic accuracy design Ultra-precision machine tool Frequency domain Error allocation

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