Lactate dehydrogenase:An important molecule involved in acetamizuril action against Eimeria tenella

作     者：LIU Li-li FEI Chen-zhong DONG Hui ZHANG Ke-yu Fu Jian-jun LI Tao XUE Fei-qun LIU Li-li;FEI Chen-zhong;DONG Hui;ZHANG Ke-yu;FU Jian-jun;LI Tao;XUE Fei-qun

作者机构：Key Laboratory of Animal ParasitologyMinistry of Agriculture/Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghai 200241P.R.China East China University of Science and TechnologyShanghai 200237P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2020年第19卷第5期

页      面：1332-1339页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the National Natural Science Foundation of China(31101857 31272607 and 31472235) 

主　　题：acetamizuril lactate dehydrogenase E.tenella second-generation merozoites 

摘      要：Lactate dehydrogenase(LDH),a vital enzyme in anaerobic glycolysis,is closely associated with the survival of *** studies of some parasites have shown that LDH exhibits unique physicochemical properties and molecular structures and may be an ideal potential target for diagnosis and drug *** this study,we aimed to investigate the effects of acetamizuril,a novel anticoccidial compound,on LDH in the second-generation merozoites of Eimeria tenella(mz-LDH).Quantitative real-time PCR,Western blot,immunofluorescence and enzyme activity assays were each applied to detect the changes of *** results indicated that the mRNA and protein levels of mz-LDH were reduced upon acetamizuril *** of mz-LDH demonstrated that considerable amount of mz-LDH was distributed around or in the nuclei of second-generation merozoites within the untreated group;in contrast,the acetamizuril-treated group had very low level of ***,LDH enzyme activity assay suggested that a decreased LDH enzyme activity in both cytoplasm and nucleus of merozoites in the acetamizuril-treated ***,the induced apoptosis in second-generation merozoites by the acetamizuril was evaluated by detecting caspase 3 activity,and acetamizuril was found to significantly increase caspase 3 *** above findings show that LDH may play an important role in the mediating the activity of acetamizuril against coccidiosis,and further investigation into this aspect might contribute to new light on the pathogenesis of *** during its interaction with acetamizuril.