Expression of Lung Surfactant Proteins SP-B and SP-C and Their Regulatory Factors in Fetal Lung of GDM Rats

作     者：Qing-miao ZHANG Wei-xiang OUYANG Xin-qun CHAI Fei-tao DENG 

作者机构：Department of Obstetrics and GynecologyUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Hepatobiliary SurgeryUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China 

出 版 物：《Current Medical Science》 (当代医学科学（英文）)

年 卷 期：2018年第38卷第5期

页      面：847-852页

核心收录：

学科分类：10[医学] 

基　　金：This work was supported by grants from the National Natural Science Foundation of China (No.30971072) and the Natural Science Foundation of Hubei Province (No.2017CFB749) 

主　　题：nitrolyogenic FOXA2 fetal lung development gestational diabetes mellitus 

摘      要：This study investigated the expression of lung surfactant proteins (SP-B and SP-C),and regulatory factors [forkhead box A2(FOXA2)and nitrolyogenic FOXA2 (N-FOXA2)]in the fetal lung of rats with gestational diabetes mellitus (GDM)in order to study the mechanism of pulmonary *** rat GDM model was established by using streptozotocin intraperitoneally in the first stage of *** were 10 rats in the GDM group,and 10 healthy rats in normal control group without any *** lungs of two groups were taken at day 21 of *** glucose levels of maternal rats and fetal rats were measured by Roche blood glucose *** histological changes in the fetal lung were observed under the light microscope in both *** SP-B,SP-C and FOXA2were determined in the fetal lung of two groups immunohistochemically. The expression levels of SP-B,SP-C,total FOXA2,FOXA2 in nucleus (n-FOXA2), N-FOXA2 proteins were detected by Western blotting,and the relative expression levels of SP-B,SP-C,FOXA2 mRNA in the fetal lung of two groups were detected by *** results showed that blood glucose levels of maternal rats and fetal rats in GDM group were higher than those in control *** light microscope revealed fetal lung development retardation in GDM *** expression of SP-B and SP-C in GDM group was significantly reduced as compared with control group (P0.05).It was concluded that GDM can cause fetal lung development and maturation disorders,and FOXA2 in fetal lung tissue decreases while nitrocellulose FOXA2 increases.