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Knowledge-embedded spatio-temporal analysis for euploidy embryos identification in couples with chromosomal rearrangements

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Chinese Medical Journal 2024年第6期137卷 694-703页

作者： Fangying Chen Xiang Xie Du cai Pengxiang Yan Chenhui Ding Yangxing Wen Yanwen Xu Feng Gao Canquan Zhou Guanbin Li qingyun Mai Reproductive Medicine Center The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510080China Guangdong Provincial Key Laboratory of Reproductive Medicine GuangzhouGuangdong 510080China School of Computer Science and Engineering Sun Yat-sen UniversityGuangzhouGuangdong 510006China Department of General Surgery(Colorectal Surgery) The Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510655China Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong 510655China Biomedical Innovation Center The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong 510655China Shanghai Artificial Intelligence Laboratory Shanghai 200232China

Background:The goal of the assisted reproductive treatment is to transfer one euploid blastocyst and to help infertile women giving birth one healthy *** algorithms have been used to assess the ploidy status of embryos derived from couples with normal chromosome,who subjected to preimplantation genetic testing for aneuploidy(PGT-A)***,it is currently unknown whether artificial intelligence model can be used to assess the euploidy status of blastocyst derived from populations with chromosomal ***:From February 2020 to May 2021,we collected the whole raw time-lapse videos at multiple focal planes from in vitro cultured embryos,the clinical information of couples,and the comprehensive chromosome screening results of those blastocysts that had received PGT ***,we developed a novel deep learning model called the Attentive Multi-Focus Selection Network(AMSNet)to analyze time-lapse videos in real time and predict blastocyst *** upon AMSNet,we integrated additional clinically predictive variables and created a second deep learning model,the Attentive Multi-Focus Video and Clinical Information Fusion Network(AMCFNet),to assess the euploidy status of *** efficacy of the AMCFNet was further tested in embryos with parental chromosomal *** receiver operating characteristic curve(ROC)was used to evaluate the superiority of the ***:A total of 4112 embryos with complete time-lapse videos were enrolled for the blastocyst formation prediction task,and 1422 qualified blastocysts received PGT-A(n=589)or PGT for chromosomal structural rearrangement(PGT-SR,n=833)were enrolled for the euploidy assessment task in this *** AMSNet model using seven focal raw time-lapse videos has the best real-time *** real-time accuracy for AMSNet to predict blastocyst formation reached above 70%on the day 2 of embryo culture,and then increased to 80%on the day 4 of embryo ***

关键词： Euploidy status Embryo Time-lapse imaging Deep learning Spatio-temporal analysis Preimplantation genetic testing for chromosomal structural rearrangement

Biocompatibility and in vitro antineoplastic drug-loaded trial of titania nanotubes prepared by anodic oxidation of a pure titanium

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Science China Chemistry 2009年第12期52卷 2161-2165页

作者： XIAO XiLin YANG LiXia GUO ManLi PAN ChunFeng cai qingyun YAO ShouZhuo State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China College of Chemistry and Chemical Engineering University of South ChinaHengyang 421001China

TiO2 nanotube (NT) arrays have been prepared by anodic oxidation of a Ti sheet,and carbon-deposited TiO2 NT arrays have been prepared by annealing TiO2 NT arrays in carbon atmosphere. The biocompatibility of the as-prepared NT arrays was investigated by observing the growth of osteosarcoma (MG-63) cells on the NT arrays. The application of the TiO2 NT arrays as a drug delivery vehicle was investigated. Both the TiO2 NTs and the carbon-modified TiO2 NTs have good biocompatibility supporting the normal growth and adhesion of MG-63 cells with no need of extracellular matrix protein coating. The one end-opened TiO2 NTs can be easily filled with drugs,working as an efficient drug delivery vehicle.

关键词： drug delivery biocompatibility titania nanotubes cell adhesion cisplatin

Autographa Californica Multiple Nucleopolyhedrovirus P48(Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles

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Virologica Sinica 2019年第6期34卷 712-721页

作者： Yan Wang qingyun cai Jiannan Chen Zhihong Huang Wenbi Wu Meijin Yuan Kai Yang State Key Laboratory of Biocontrol Sun Yat-sen University

Our previous study has shown that the Autographa californica multiple nudeopolyhedrovirus(AcMNPV)p48(acl03)gene is essential for the nuclear egress of nucleocapsids and the formation of occlusion-derived virions(ODVs).However,the exact role of p48 in the morphogenesis of ODVs remains *** this study,we demonstrated that p48 was required for the efficient formation of intranuclear *** further understand its functional role in intranuclear microvesicle formation,we characterized the distribution of the P48 protein,which was found to be associated with the nucleocapsid and envelope fractions of both budded virions and *** AcMNPV-infected cells,P48 was predominantly localized to nucleocapsids in the virogenic stroma and the nucleocapsids enveloped in ODVs,with a limited but discernible distribution in the plasma membrane,nuclear envelope,intranuclear microvesicles,and ODV ***,coimmunoprecipitation assays showed that among the viral proteins required for intranuclear microvesicle formation,F48 associated with Ac93 in the absence of viral infection.

关键词： Baculovirus Intranuclear microvesicle formation Protein association Ac93

Ultrasensitive Label-free Detection of miRNA with Asymmetric Hairpin Probe, Exonuclease I and SYBR Green I

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Chemical Research in Chinese Universities 2015年第2期31卷 244-248页

作者： LIU Yingxin SU Hongyan LONG Jiabao CAO Qingfeng YAN Shuya MENG Xiangxian cai qingyun College of Biology State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha 410082 P. R. China Department of Bioengineering and Environment Science Changsha University Changsha 410003 P. R. China

Detection of miRNAs presents a particular challenge because of their limited size, high sequence homo- logy and greatly various expression level. In this work, an ultrasensitive, label-free and isothermal miRNA detection was developed based on asymmertric hairpin probe, exonuclease I（Exo I） and SYBR Green I. The method employed asymmetric hairpin probe to perform cycled polymerization and Exo I to reduce background signal. In the presence of the target miRNA, the target triggers probe-mediated cycled polymerization reactions to generate lots of dsDNA products. The dsDNA product effectively prevents itself from being degraded by Exo I and permitted the insertion of more fluorescence dye into it to enlarge the fluorescence signal. In the absence of the target miRNA, there was no probe-mediated polymerization reaction, and the probe was digested by Exo I added, which minimized the intercala- tion of fluorescence dye to reduce the background signal. The combination of the probe-mediated cycled polymeriza- tion with the Exo 1-assisted background reduction allows us to achieve a detection limit of 5× 10^-18 mol/L. Owing to its ultrasensitivity, excellent specificity, convenience and low-cost, this method might hold out great promise in miRNA detection.

关键词： Ultrasensitive miRNA Asymmetric hairpin probe Exonuclease I

Super-bright Eu,Sm,Mn-doped CaS UCNPs: Applications in bioimaging and ultra-sensitive detection

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Super-bright Eu,Sm,Mn-doped CaS UCNPs: Applications in bioim...

不同的降压治疗方案对高血压脑出血病人神经功能、炎症反应及预后的影响

中国化学会第十三届全国分析化学年会

作者： Yanli Zhu Jikai Wang qingyun cai State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University

Upconversion nanoparticles(UCNPs) have aroused intensive concerns for their upconversion luminescence(UCL) and unique optical properties compared with the conventional downconversion luminescence materials [1]. However, the low UCL quantum yield precludes its applications. In this work, CaS:Eu,Sm,Mn UCNPs with a ultra-high UCL quantum yield of nearly 60% were synthesized, and applied to latent fingerprint detection, deep tissue imaging, and in vivo bioimaging. Such applications revealed rather lower background interference and much higher imaging depth when compared with tranditional NaYF based UCNPs [2]. Moreover, UCNP nanoparticle-based paper test strip for one-shot quantitative measurement of sulfite concentration was prepared and a portable smartphone-based reader was developed for in-field determination of sulfite. Significantly the integrated sulfite detection system featured high sensitivity(0.002 μg/mL) and facile operation, Collection of intense UCL signal instead of colorimetry ensured a direct analyte determination without the need for separation and pretreatment. The design principles are general in nature, and so it can be tailored for the detection and quantification of a variety of other analytes.

关键词： Upconversion luminescence Rare-earth doped nanoparticles Bioimaging Sulfite detection

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安徽医药 2022年第3期26卷 595-599页

作者：贺智杰蔡青云信阳市中心医院重症医学科河南信阳464000 驻马店市中心医院急诊科河南驻马店463000

目的探讨强化降压与常规降压治疗对高血压脑出血(HICH)病人神经功能、炎症反应及病人预后的影响。方法选取2016年3月至2019年3月信阳市中心医院收治的HICH病人108例,采用随机数字表法分为强化组和常规组各54例,强化组采用早期强化降血... 详细信息

目的探讨强化降压与常规降压治疗对高血压脑出血(HICH)病人神经功能、炎症反应及病人预后的影响。方法选取2016年3月至2019年3月信阳市中心医院收治的HICH病人108例,采用随机数字表法分为强化组和常规组各54例,强化组采用早期强化降血压治疗(3 h内将病人收缩压降至140 mmHg以下,并以120~140 mmHg维持1周),常规组目标降压为收缩压160~180 mmHg,平均动脉压100~130 mmHg;对比两组病人的美国国立卫生研究院卒中量表(NIHSS)评分、格拉斯哥预后评分(GOS)、治疗前后的血浆血管性假血友病因子(vWF)、核因子-κB(NF-κB)、高迁移率族蛋白-1(HMGB-1)、C反应蛋白(CRP)、脑水肿体积变化。结果治疗时间与降压方案存在交互作用,而时间因素对病人NIHSS评分变化及脑水肿体积变化的作用均随降压方案的不同而差异有统计学意义。随着治疗时间的延长,两组病人的NIHSS评分均呈逐渐降低的趋势,在治疗7 d、14 d时刻,强化组病人的NIHSS评分[(6.11±2.50)分、(3.89±1.04)分]均低于对应时间点的常规组[(7.64±2.39)分、(5.08±1.30)分](P<0.05);治疗7 d时,强化组与常规组病人的脑水肿体积[(27.55±6.20)mL、(30.20±5.84)mL]较治疗3 d时[(19.64±5.52)mL、(22.50±4.85)mL]均有所增大,在治疗14 d时刻,强化组与常规组病人的脑水肿体积[(9.30±3.75)mL、(11.66±4.10)mL]均显著缩小(P<0.05),强化组的脑水肿体积[(9.30±3.75)mL]小于常规组[(11.66±4.10)mL](P<0.05);治疗7 d后,强化组的血浆vWF[(96.3±14.2)μg/L]、NF-κB[(851.1±133.2)μg/L]、HMGB-1[(1.64±0.62)mg/L]、CRP[(7.2±2.1)mg/L]水平小于常规组[(115.0±19.4)μg/L、(926.5±143.0)μg/L、(2.33±0.86)mg/L、(9.5±2.8)mg/L](P<0.05);治疗3个月后,强化组病人的预后良好率(70.37%)高于常规组(50.00%)(P<0.05)。结论幕上少量出血的HICH病人早期采用强化降压治疗对于减轻脑水肿程度及炎症反应,促进病人神经功能恢复,改善预后具有一定的积极意义。

关键词：颅内出血,高血压性降压,控制性强化降压神经功能预后

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双层结构检验方法的比较研究

统计与决策 2019年第17期35卷 22-25页

作者：蔡晴云杨晶厦门大学国际学院

在海量数据的假设检验中,传统的假设检验方法和错误控制率会导致重要的信号被遗漏掉。文章提出利用数据结构进行分层检验,并结合较为宽松的错误发现率(FDR)进行控制;重点讨论了双层结构检验中检验阈值的选择,对比单层和双层结构检验方法... 详细信息

在海量数据的假设检验中,传统的假设检验方法和错误控制率会导致重要的信号被遗漏掉。文章提出利用数据结构进行分层检验,并结合较为宽松的错误发现率(FDR)进行控制;重点讨论了双层结构检验中检验阈值的选择,对比单层和双层结构检验方法,分析它们的优点并利用Monte Carlo数值模拟它们在错误控制率和检验灵敏度上的表现。

关键词：假设检验 FDR 分层检验 Monte Carlo模拟

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列车风致脉动力下声屏障的动力学性能

北京航空航天大学学报 2009年第4期35卷 505-508页

作者：赵丽滨龙丽平蔡庆云北京航空航天大学宇航学院北京100191 北京航空航天大学航空科学与工程学院北京100191 铁道第三勘察设计院机械动力和环境工程设计处天津300251

基于通用有限元分析软件ANSYS,根据京津城际快速列车客运专线桥梁上声屏障结构的设计方案建立有限元模型,对声屏障结构的固有频率进行了研究并提出声屏障结构的简化计算模型.综合考虑高速列车运行过程中声屏障结构的动力学环境,计算在... 详细信息

基于通用有限元分析软件ANSYS,根据京津城际快速列车客运专线桥梁上声屏障结构的设计方案建立有限元模型,对声屏障结构的固有频率进行了研究并提出声屏障结构的简化计算模型.综合考虑高速列车运行过程中声屏障结构的动力学环境,计算在风压、列车运行过程中产生的空气脉动力等共同作用下的声屏障结构的动态响应,阐述了声屏障结构各组件的振动规律,并提出声屏障结构设计需要考虑的问题.

关键词：声屏障空气脉动力固有频率动力学响应

Toll样受体2、Toll样受体4 mRNA和蛋白在急性出血坏死性胰腺炎肝损伤中的表达及意义

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安徽医药 2022年第1期26卷 82-86页

作者：蔡青云杨留生张贺川驻马店市中心医院急诊科河南驻马店463000

目的探讨Toll样受体2(TLR2)、TLR4信使核糖核酸(mRNA)和蛋白在急性出血坏死性胰腺炎(AHNP)肝损伤中的表达及意义。方法2019年6月至12月选取健康SD大鼠(郑州大学实验动物中心)60只为研究对象,采用随机数字表法分为模型组和对照组,每组30... 详细信息

目的探讨Toll样受体2(TLR2)、TLR4信使核糖核酸(mRNA)和蛋白在急性出血坏死性胰腺炎(AHNP)肝损伤中的表达及意义。方法2019年6月至12月选取健康SD大鼠(郑州大学实验动物中心)60只为研究对象,采用随机数字表法分为模型组和对照组,每组30只,同时模型组和对照组再随机分为3 h组、6 h组和12 h组各10只。模型组采用牛磺胆酸钠建立AHNP模型,对照组注射生理盐水,检测各组血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和淀粉酶,qRT-PCR和Western blotting检测肝脏组织TLR2、TLR4 mRNA和蛋白表达,HE染色观察肝脏组织。结果模型组造模后3 h组、6 h组和12 h组血清ALT[(170.02±32.21)U/L、(310.01±80.02)U/L、(720.11±112.28)U/L]、AST[(450.13±100.21)U/L、(980.12±132.21)U/L、(1100.32±145.03)U/L]和淀粉酶[(7099.27±1209.02)U/L、(11010.19±1200.01)U/L、(17003.21±2007.82)U/L],肝脏组织TLR2和TLR4 mRNA及蛋白相对表达量[TLR2 mRNA(0.962±0.201)、(1.201±0.223)、(1.501±0.212),TLR2蛋白(0.713±0.091)、(1.102±0.100)、(1.343±0.121);TLR4 mRNA(1.701±0.332)、(2.011±0.350)、(2.562±0.313),TLR4蛋白(1.210±0.170)、(1.446±0.182)、(1.788±0.190)]均逐渐增高(P<0.001),且均明显高于对照组[ALT(84.40±14.92)U/L、(85.50±15.20)U/L、(84.19±14.49)U/L;AST(170.02±20.01)U/L、(168.82±21.14)U/L、(171.14±20.11)U/L;淀粉酶(1102.00±183.29)U/L、(1154.49±190.04)U/L、(1160.03±186.27)U/L;TLR2 mRNA(0.030±0.010)、(0.031±0.011)、(0.029±0.009),TLR2蛋白(0.021±0.008)、(0.020±0.009)、(0.022±0.010);TLR4 mRNA(0.882±0.181)、(0.880±0.170)、(0.883±0.179),TLR4蛋白(0.961±0.192)、(0.967±0.181)、(0.966±0.180)](P<0.001)。肝组织TLR2、TLR4 mRNA表达与血清ALT、AST和淀粉酶呈正相关(P<0.05),TLR2、TLR4蛋白表达与血清ALT、AST和淀粉酶呈正相关(P<0.05);模型组造模3 h后可见肝细胞气球样变性,6 h后炎性细胞浸润汇管区,小叶周边细胞发生嗜酸性变性,12 h后出现大片状出血坏死及灶性坏死。结论TLR2、TLR4 mRNA和蛋白在AHNP肝损伤组织中的表达升高,可能在肝脏损伤过程中有重要作用。

关键词：胰腺炎,急性坏死性肝功能不全丙氨酸转氨酶 Toll样受体2 Toll样受体4 信使核糖核酸蛋白大鼠,Sprague-Dawley