Autographa Californica Multiple Nucleopolyhedrovirus P48(Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles

作     者：Yan Wang Qingyun Cai Jiannan Chen Zhihong Huang Wenbi Wu Meijin Yuan Kai Yang 

作者机构：State Key Laboratory of Biocontrol Sun Yat-sen University 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2019年第34卷第6期

页      面：712-721页

核心收录：

学科分类：07[理学] 070202[理学-粒子物理与原子核物理] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China (31572056 and 31872025) the Key Project of Natural Science Foundation of Guangdong Province (2018B030311018) the National Key R&D Program of China (2017YFD0200404) the Guangzhou Science and Technology Project (201707020003) 

主　　题：P48 Baculovirus Intranuclear microvesicle formation Protein association Ac93 

摘      要：Our previous study has shown that the Autographa californica multiple nucleopolyhedrovirus(AcMNPV) p48(ac103)gene is essential for the nuclear egress of nucleocapsids and the formation of occlusion-derived virions(ODVs). However,the exact role of p48 in the morphogenesis of ODVs remains unknown. In this study, we demonstrated that p48 was required for the efficient formation of intranuclear microvesicles. To further understand its functional role in intranuclear microvesicle formation, we characterized the distribution of the P48 protein, which was found to be associated with the nucleocapsid and envelope fractions of both budded virions and ODVs. In Ac MNPV-infected cells, P48 was predominantly localized to nucleocapsids in the virogenic stroma and the nucleocapsids enveloped in ODVs, with a limited but discernible distribution in the plasma membrane, nuclear envelope, intranuclear microvesicles, and ODV envelope. Furthermore,coimmunoprecipitation assays showed that among the viral proteins required for intranuclear microvesicle formation, P48 associated with Ac93 in the absence of viral infection.