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Chemical biology investigation of a triple-action,smart-decomposition antimicrobial booster based-combination therapy against“ESKAPE”pathogens

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Science China Chemistry 2024年第9期67卷 3071-3082页

作者： Min Wang Huangsheng Pu Yangfan Xu Chenxuan Wu Yuanxin Gu qingyun cai Guoxing Yin Peng Yin Chunhui Zhang Wing-Leung Wong Muyang Wan Yugang Bai Xinxin Feng State Key Laboratory of Chem-/Bio-Sensing and Chemometrics Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biologyand School of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China College of Biology Hunan UniversityChangsha 410082China College of Advanced Interdisciplinary Studies&Hunan Provincial Key Laboratory of Novel Nano Optoelectronic Information Materials and Devices National University of Defense TechnologyChangsha 410073China Nanhu Laser Laboratory National University of Defense TechnologyChangsha 410073China Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Ministry of Education) College of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha 410081China State Key Laboratory of Chemical Biology and Drug Discovery Department of Applied Biology and Chemical TechnologyThe Hong Kong Polytechnic UniversityHong Kong SAR 999077China Hunan Provincial Key Laboratory of Anti-Resistance Microbial Drugs The Third Hospital of Changsha(The Affiliated Changsha Hospital of Hunan University)Changsha 410015China

The global antibiotic resistance crisis necessitates urgent *** innovative approach involves potentiating antibiotics and non-antibiotic drugs with adjuvants or boosters.A major drawback of these membrane-active boosters is their limited biocompatibility,as they struggle to differentiate between prokaryotic and eukaryotic *** study reports the chemical biology investigation of a dual-action oligoamidine(OA1)booster with a glutathione-triggered decomposition ***1,when combined with other antimicrobial molecules,exhibits a triple-targeting mechanism including cell membrane disruption,DNA targeting,and intracellular enzyme *** multi-targeting mechanism not only enhances the in vitro and in vivo eradication of antibiotic-resistant“ESKAPE”pathogens,but also suppresses the development of bacterial ***,OA1 maintains its activity in bacterial cells by creating an oxidative environment,while it quickly decomposes in mammalian cells due to high glutathione *** mechanistic insights and design principles may provide a feasible approach to develop novel antimicrobial agents and effective anti-resistance combination therapies.

关键词： antimicrobial booster oligoamidine membrane disruption DNA targeting triggered degradation anti-resistance

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Biocompatibility and in vitro antineoplastic drug-loaded trial of titania nanotubes prepared by anodic oxidation of a pure titanium

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Science China Chemistry 2009年第12期52卷 2161-2165页

作者： XIAO XiLin YANG LiXia GUO ManLi PAN ChunFeng cai qingyun YAO ShouZhuo State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China College of Chemistry and Chemical Engineering University of South ChinaHengyang 421001China

TiO2 nanotube (NT) arrays have been prepared by anodic oxidation of a Ti sheet,and carbon-deposited TiO2 NT arrays have been prepared by annealing TiO2 NT arrays in carbon atmosphere. The biocompatibility of the as-prepared NT arrays was investigated by observing the growth of osteosarcoma (MG-63) cells on the NT arrays. The application of the TiO2 NT arrays as a drug delivery vehicle was investigated. Both the TiO2 NTs and the carbon-modified TiO2 NTs have good biocompatibility supporting the normal growth and adhesion of MG-63 cells with no need of extracellular matrix protein coating. The one end-opened TiO2 NTs can be easily filled with drugs,working as an efficient drug delivery vehicle.

关键词： drug delivery biocompatibility titania nanotubes cell adhesion cisplatin

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Autographa Californica Multiple Nucleopolyhedrovirus P48(Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles

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Virologica Sinica 2019年第6期34卷 712-721页

作者： Yan Wang qingyun cai Jiannan Chen Zhihong Huang Wenbi Wu Meijin Yuan Kai Yang State Key Laboratory of Biocontrol Sun Yat-sen University

Our previous study has shown that the Autographa californica multiple nucleopolyhedrovirus(AcMNPV) p48(ac103)gene is essential for the nuclear egress of nucleocapsids and the formation of occlusion-derived virions(ODVs). However,the exact role of p48 in the morphogenesis of ODVs remains unknown. In this study, we demonstrated that p48 was required for the efficient formation of intranuclear microvesicles. To further understand its functional role in intranuclear microvesicle formation, we characterized the distribution of the P48 protein, which was found to be associated with the nucleocapsid and envelope fractions of both budded virions and ODVs. In Ac MNPV-infected cells, P48 was predominantly localized to nucleocapsids in the virogenic stroma and the nucleocapsids enveloped in ODVs, with a limited but discernible distribution in the plasma membrane, nuclear envelope, intranuclear microvesicles, and ODV envelope. Furthermore,coimmunoprecipitation assays showed that among the viral proteins required for intranuclear microvesicle formation, P48 associated with Ac93 in the absence of viral infection.

关键词： P48 Baculovirus Intranuclear microvesicle formation Protein association Ac93

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CuInS2/ZnS QDs-based fluorescent probe for detection of Cu2+

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Progress in Natural Science:Materials International 2023年第4期33卷 495-500页

作者： Haiyan Kang Hongchao Geng Sisi Shi Zhongxian Song Yuting Dong Xiaole Yan Chaohai Wang Yanli Mao qingyun cai Henan Province Key Laboratory of Water Pollution Control and Rehabilitation Technology Henan University of Urban Construction State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University

High concentrations of Cu2+exceeding what the required level for the cellular process can changes the properties of protein, affects the normal function of the body, but also affect the body’s metabolism, so that the body’s immunity appears to decline. Detection of Cu2+is important for the environmental monitoring. Here, a fluorescence probe that uses heavy metal-free and properties-superior CuInS2/ZnS was proposed for detection of Cu2+. The fluorescence intensity of CuInS2/ZnS quantum dots(QDs) decreased obviously after Cu2+were added,and the intensity with the concentration of Cu2+showed excellent linear relation within the scope of 0–50 μM, the LOD down to 0.45 μM, a possible detection mechanism was analyzed. The QDs was further applied to the detection of Cu2+in drinking water, manifesting the foreground of the current strategy.

关键词： CuInS_2/ZnS QDs Fluorescence probe Detection Cu~(2+)

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Ultrasensitive Label-free Detection of miRNA with Asymmetric Hairpin Probe, Exonuclease I and SYBR Green I

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Chemical Research in Chinese Universities 2015年第2期31卷 244-248页

作者： LIU Yingxin SU Hongyan LONG Jiabao CAO Qingfeng YAN Shuya MENG Xiangxian cai qingyun College of Biology State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha 410082 P. R. China Department of Bioengineering and Environment Science Changsha University Changsha 410003 P. R. China

Detection of miRNAs presents a particular challenge because of their limited size, high sequence homo- logy and greatly various expression level. In this work, an ultrasensitive, label-free and isothermal miRNA detection was developed based on asymmertric hairpin probe, exonuclease I（Exo I） and SYBR Green I. The method employed asymmetric hairpin probe to perform cycled polymerization and Exo I to reduce background signal. In the presence of the target miRNA, the target triggers probe-mediated cycled polymerization reactions to generate lots of dsDNA products. The dsDNA product effectively prevents itself from being degraded by Exo I and permitted the insertion of more fluorescence dye into it to enlarge the fluorescence signal. In the absence of the target miRNA, there was no probe-mediated polymerization reaction, and the probe was digested by Exo I added, which minimized the intercala- tion of fluorescence dye to reduce the background signal. The combination of the probe-mediated cycled polymeriza- tion with the Exo 1-assisted background reduction allows us to achieve a detection limit of 5× 10^-18 mol/L. Owing to its ultrasensitivity, excellent specificity, convenience and low-cost, this method might hold out great promise in miRNA detection.

关键词： Ultrasensitive miRNA Asymmetric hairpin probe Exonuclease I

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Super-bright Eu,Sm,Mn-doped CaS UCNPs: Applications in bioimaging and ultra-sensitive detection

Super-bright Eu,Sm,Mn-doped CaS UCNPs: Applications in bioim...

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中国化学会第十三届全国分析化学年会

作者： Yanli Zhu Jikai Wang qingyun cai State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University

Upconversion nanoparticles(UCNPs) have aroused intensive concerns for their upconversion luminescence(UCL) and unique optical properties compared with the conventional downconversion luminescence materials [1]. However, the low UCL quantum yield precludes its applications. In this work, CaS:Eu,Sm,Mn UCNPs with a ultra-high UCL quantum yield of nearly 60% were synthesized, and applied to latent fingerprint detection, deep tissue imaging, and in vivo bioimaging. Such applications revealed rather lower background interference and much higher imaging depth when compared with tranditional NaYF based UCNPs [2]. Moreover, UCNP nanoparticle-based paper test strip for one-shot quantitative measurement of sulfite concentration was prepared and a portable smartphone-based reader was developed for in-field determination of sulfite. Significantly the integrated sulfite detection system featured high sensitivity(0.002 μg/mL) and facile operation, Collection of intense UCL signal instead of colorimetry ensured a direct analyte determination without the need for separation and pretreatment. The design principles are general in nature, and so it can be tailored for the detection and quantification of a variety of other analytes.

关键词： Upconversion luminescence Rare-earth doped nanoparticles Bioimaging Sulfite detection

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双层结构检验方法的比较研究

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统计与决策 2019年第17期35卷 22-25页

作者：蔡晴云杨晶厦门大学国际学院

在海量数据的假设检验中,传统的假设检验方法和错误控制率会导致重要的信号被遗漏掉。文章提出利用数据结构进行分层检验,并结合较为宽松的错误发现率(FDR)进行控制;重点讨论了双层结构检验中检验阈值的选择,对比单层和双层结构检验方法... 详细信息

在海量数据的假设检验中,传统的假设检验方法和错误控制率会导致重要的信号被遗漏掉。文章提出利用数据结构进行分层检验,并结合较为宽松的错误发现率(FDR)进行控制;重点讨论了双层结构检验中检验阈值的选择,对比单层和双层结构检验方法,分析它们的优点并利用Monte Carlo数值模拟它们在错误控制率和检验灵敏度上的表现。

关键词：假设检验 FDR 分层检验 Monte Carlo模拟

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列车风致脉动力下声屏障的动力学性能

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北京航空航天大学学报 2009年第4期35卷 505-508页

作者：赵丽滨龙丽平蔡庆云北京航空航天大学宇航学院北京100191 北京航空航天大学航空科学与工程学院北京100191 铁道第三勘察设计院机械动力和环境工程设计处天津300251

基于通用有限元分析软件ANSYS,根据京津城际快速列车客运专线桥梁上声屏障结构的设计方案建立有限元模型,对声屏障结构的固有频率进行了研究并提出声屏障结构的简化计算模型.综合考虑高速列车运行过程中声屏障结构的动力学环境,计算在... 详细信息

基于通用有限元分析软件ANSYS,根据京津城际快速列车客运专线桥梁上声屏障结构的设计方案建立有限元模型,对声屏障结构的固有频率进行了研究并提出声屏障结构的简化计算模型.综合考虑高速列车运行过程中声屏障结构的动力学环境,计算在风压、列车运行过程中产生的空气脉动力等共同作用下的声屏障结构的动态响应,阐述了声屏障结构各组件的振动规律,并提出声屏障结构设计需要考虑的问题.

关键词：声屏障空气脉动力固有频率动力学响应

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高速铁路列车自动清洗设备研究

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都市快轨交通 2006年第5期19卷 98-101页

作者：蔡庆云铁道第三勘察设计院天津300251

列车整备是高速铁路保障行车安全的重要工作。论述国内外高速列车清洗设备的现状,介绍高速(快速)动车组自动清洗设备的研究成果,分析工艺流程、刷组设备、自动控制方案及在工程实际中的成功运用。

关键词：高速列车清洗设备自动清洗研究

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磁固相萃取结合化学计量学分析水样中多环芳烃

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环境科学与技术 2018年第10期41卷 162-168页

作者：张斌刘晶晶蔡青云长沙环境保护职业技术学院环境监测系湖南长沙410004 湖南大学化学化工学院湖南长沙410082

利用萘基的稠环结构和π-π相互作用,运用异氰酸萘酯和羟基反应特性,将萘基引入包硅的Fe_3O_4磁纳米粒子表面,制备了萘基功能化的核-壳结构磁性吸附材料Fe_3O_4@SiO_2@Nap,并研究其对多环芳烃的萃取性能。采用化学计量学对多环芳烃的GC... 详细信息

利用萘基的稠环结构和π-π相互作用,运用异氰酸萘酯和羟基反应特性,将萘基引入包硅的Fe_3O_4磁纳米粒子表面,制备了萘基功能化的核-壳结构磁性吸附材料Fe_3O_4@SiO_2@Nap,并研究其对多环芳烃的萃取性能。采用化学计量学对多环芳烃的GC-MS质谱图进行分辨,结合质谱库相似度匹配对各组分进行定性。从35个色谱峰中获得了22种化学成分,其中多环芳烃12种,其余10种为含有苯基或双键官能团化合物。实验证明Fe_3O_4@SiO_2@Nap纳米粒子具有选择性、稳定性、疏水性、快速富集与分离、萃取过程简单等优点,不仅能富集多环芳烃,也能富集含有π键结构的化合物。结合化学计量学分析方法,不仅降低了对色谱柱的要求,而且能快速、全面检测环境水样中的复杂未知多环芳烃。

关键词：多环芳烃磁纳米粒子萃取气相色谱-质谱联用仪(GC-MS) 化学计量学

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