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Animal Models and Experimental Medicine 2023年第2期6卷 111-119页

作者： Yajuan Wang qingrong zhang Shuchi zhang Jiangtao Qi Li Li Department of Anesthesiology Huashan HospitalFudan UniversityShanghaiChina Department of Anesthesiology Tongji HospitalTongji UniversityShanghaiChina

Background:TTC(2,3,5-triphenyltetrazolium chloride)staining is the most commonly used method in identifying and assessing cerebral infarct volumes in the transient middle cerebral artery occlusion *** that microglia exhibit different morphologies in different regions after ischemic stroke,we demonstrate the superiority and necessity of using TTC-stained brain tissue to analyze the expression of various proteins or genes in different regions based on microglia ***:We compared brain tissue(left for 10 min on ice)from the improved TTC staining method with penumbra from the traditional sampling *** identified the feasibility and necessity of the improved staining method using real time(RT)-PCR,Western blot,and immunofluorescence ***:There was no protein and RNA degradation in the TTC-stained brain tissue ***,the TREM2 specifically expressed on the microglia showed a significant difference between two groups in the penumbra ***:TTC-stained brain tissue can be used for molecular biology experiments without any *** addition,TTC-stained brain tissue shows greater superiority due to its precise positioning.

关键词： ischemic brain injury microglia transient middle cerebral artery occlusion TTC staining

Studying structure and functions of cell membranes by single molecule biophysical techniques

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Biophysics Reports 2021年第5期7卷 384-398页

作者： qingrong zhang Siying Li Yu Yang Yuping Shan Hongda Wang School of Chemistry and Life Science Advanced Institute of Materials Science Changchun University of Technology State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences

Cell membranes are complicated multicomponent structures, related to many basic cellular processes,such as substance transporting, energy conversion, signal transduction, mechanosensing, cell adhesion and so on. However, cell membranes have long been difficult to study at a single-molecule level due to their complex and dynamic properties. During the last decades, biophysical imaging techniques, such as atomic force microscopy and super-resolution fluorescent microscopy, have been developed to study biological structures with unprecedented resolution, enabling researchers to analyze the composition and distribution of membrane proteins and monitor their specific functions at single cell/molecule level. In this review, we highlight the structure and functions of cell membranes based on up-to-date biophysical techniques. Additionally, we describe the recent advances in force-based detecting technology, which allow insight into dynamic events and quantitativelymonitoring kinetic parameters for trans-membrane transporting in living cells.

关键词： Atomic force microscopy Single molecule Cell membrane Super-resolution fluorescence microscopy Single molecule force spectroscopy

Experimental verifications on chemical carcinogenesis, a bifunctional alkvlation between DNA interstrands

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Chinese Science Bulletin 2000年第12期45卷 1075-1079页

作者： DAI Qianhuan, zhang qingrong, WANG Lihui, PEI Shu, WANG Qing &QUBoCenter for Chemistry and Bioengineering of Cancer, Beijing Polytechnic University, Beijing 100022, China Center for Chemistry and Bioengineering of Cancer Beijing Polytechnic University Beijing China

It is evidenced by the filter elution method that two carcinogenic aromatic hydrocarbons, benzo[a]pyrene and dibenzo[a,h]anthracene, two carcinogenic metal salts, beryllium chloride and cadmium chloride, four carcinogenic aromatic amines, 2-aminofluorene, p-naphthylamine, 4-aminobiphenyl and benzidine, can all induce DNA interstrand and DNA-protein cross-link in L12io culture. However, under the same condition, the corresponding non-carcinogenic compounds, including benzo[k]fluorancene, anthracene, magnesium chloride, zinc chloride, a-naphthylamine, 2-aminobiphenyl and m-toluidine, cannot produce any cross-link adducts. All these results are consistent with the di-region theory that carcinogens are bio-bifunctional alkylation agents. This method can also be used to discriminate carcinogens and non-carcinogens.

关键词： mechanism of chemical carcinogenesis di-region theory DNA interstrand cross-link aromatic amines poiycyclic aromatic hydrocarbons beryllium salt cadmium salt.

Design and Implementation of an Ultrasonic Heat Meter Based on MSP430F437

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Computer Aided Drafting,Design and Manufacturing 2011年第2期21卷 32-39页

作者： LI Yue HE Yu-zhu ZHOU Hai-li CHEN Gong zhang qingrong School of Instrumentation Science and Opto-Elctronics Engineering Beijing University of Aeronautics and Astronautic Beijing 100191 China

Flowing with the reform of the hot water heating method in China, heat meter will enter into households in the near future. A portable ultrasonic heat meter is designed in this paper. The meter uses chip microprocessor MSP430F437 as the data process core, and uses ultrasonic flow sensor to measure flow rate of the hot water, and capture input and output temperatures of the hot water using the thermal resistance sensor Ptl000, and then household energy consumption is calculated via temperature difference between input temperature and output temperature of the hot water multiplied by volume of hot water that is calculated though flow rate integration of hot water. In order to test the performance of the proposed heat meter, experiments is carried out. Both the temperature and flow measurement results satisfy the requirements of accuracy and the heat meter is effective in the heat measurement.

关键词： heat meter MSP430F437 ultrasonic flow sensor thermal resistance sensor

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热喷涂对模具钢表面性能的改善与研究

热加工工艺 2014年第14期43卷 156-159页

作者：张庆荣潍坊科技学院山东寿光262700

通过电弧喷涂工艺在8Cr3模具钢表面制备了FeCrAl涂层。利用扫描电镜、能谱分析仪对涂层表面、截面形貌、元素进行分析,利用MH-3型显微硬度计对FeCrAl涂层的平均硬度进行测定;将试样静置于720℃的熔融ZL101A铝液30 min、1 h和2 h,比较各... 详细信息

通过电弧喷涂工艺在8Cr3模具钢表面制备了FeCrAl涂层。利用扫描电镜、能谱分析仪对涂层表面、截面形貌、元素进行分析,利用MH-3型显微硬度计对FeCrAl涂层的平均硬度进行测定;将试样静置于720℃的熔融ZL101A铝液30 min、1 h和2 h,比较各试样的熔损情况。结果表明:涂层与模具钢基体结合部位没有十分明显的界线,部分区域相互机械咬合,FeCrAl涂层内部粒子相互交错成波浪状堆叠,涂层整体组织致密;模具表面喷涂的FeCrAl涂层,其平均维氏硬度可达282.6 HV0.5;试样经电弧喷涂FeCrAl涂层处理后,随铝熔蚀时间的延长,熔蚀速率明显偏低。未喷涂处理的模具钢熔蚀后在界面附近形成了新相Fe2A15。

关键词：电弧喷涂模具钢耐磨性铝熔蚀

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基于图像处理的汽车指针仪表检测研究

计算机应用与软件 2014年第8期31卷 219-221,252页

作者：徐洋张清蓉重庆邮电大学汽车电子与嵌入式工程研究中心重庆400065

研究一种基于图像处理技术的汽车指针仪表自动检测系统。在OpenCV的编程环境中,利用图像处理技术,实现图像细化、图像二值化等图像预处理。采用一种改进的概率霍夫变换与RGB颜色通道相结合的方法,实现汽车仪表的指针检测。这种方法能减... 详细信息

研究一种基于图像处理技术的汽车指针仪表自动检测系统。在OpenCV的编程环境中,利用图像处理技术,实现图像细化、图像二值化等图像预处理。采用一种改进的概率霍夫变换与RGB颜色通道相结合的方法,实现汽车仪表的指针检测。这种方法能减少运算量,并准确地识别出指针直线。

关键词：图像处理图像细化概率霍夫换 RGB颜色通道

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X80管线钢的应力-应变特性及失效判据

热加工工艺 2016年第20期45卷 118-120,123页

作者：张庆荣潍坊科技学院汽车工程学院山东寿光262700

通过对X80管线钢进行不同方向的静力拉伸试验,研究了其应力-应变特性。结果表明:X80管线钢具有连续屈服性,没有明显的屈服平台,有较低的屈强比和较大的均匀塑性伸长率。根据X80管线钢的力学性能特点,研究了X80管线钢基于应力的失效判据... 详细信息

通过对X80管线钢进行不同方向的静力拉伸试验,研究了其应力-应变特性。结果表明:X80管线钢具有连续屈服性,没有明显的屈服平台,有较低的屈强比和较大的均匀塑性伸长率。根据X80管线钢的力学性能特点,研究了X80管线钢基于应力的失效判据和基于应变的失效判据。基于Mises屈服准则和Lame(拉美)公式建立了X80管线钢失效的临界应力计算方程,并给出了X80管线钢的应变失效准则。

关键词： X80管线钢应力-应变曲线失效判据

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还原剂对钒合金化非调质钢组织和性能的影响

铸造技术 2017年第11期38卷 2613-2615,2625页

作者：张庆荣潍坊科技学院山东潍坊262700

通过添加还原剂提高钒的收得率,增加钒在45钢中的合金化率制备非调质钢。采用化学成分、力学性能、金相和扫描电镜等分析,研究了不同还原剂对V_2O_5中V收得率的影响,探讨其对钒合金化非调质钢性能的影响。结果表明,Al还原收得率显著高... 详细信息

通过添加还原剂提高钒的收得率,增加钒在45钢中的合金化率制备非调质钢。采用化学成分、力学性能、金相和扫描电镜等分析,研究了不同还原剂对V_2O_5中V收得率的影响,探讨其对钒合金化非调质钢性能的影响。结果表明,Al还原收得率显著高于Si。(Al+Mg)复合还原比单一还原效果要优,当复合还原剂中Al为61.5%、Mg为38.5%时,所制备的钒合金化调质钢力学性能最佳,抗拉强度和屈服强度分别为540 MPa和410 MPa。

关键词：还原剂 V2O5 合金化非调质钢组织和性能

Investigation of Instability of Lattice of Bi_2Sr_2CaCu_2O_x

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Journal of Materials Science & Technology 1996年第3期12卷 175-179页

作者： Han zhang and qingrong FENG (Dept. of Physics, Peking University, Beijing 100871, China)Y.Zhao(School of Materials Science and Engineering, University of New South Wales, Sydney 2052, NSW, Australia)F.Ritter and W.Assmus (Physikalisches Institut, J.W.Goet UNIV NEW S WALES SCH MAT SCI & ENGNSYDNEYNSW 2052AUSTRALIA UNIV FRANKFURT INST PHYSD-60054 FRANKFURTGERMANY

Bi2Sr2CaCu2Ox superconductor was studied by differential scanning calorimetry (DSC) and thermogravimetry (TG) in different atmospheres. It is discovered that there is a thermal anomaly in the DSC curve, associated with a weight loss in the TG curve before the melting of the sample. Careful thermal analysis and high temperature X-ray diffraction reveal that the thermal anomaly and the weight loss show an instability of the crystalline Iattice. By annealing the sample in oxygen and argon, respectively and then by TC measurement and thermal analysis, the coincidence of transition temperature with the instability is found. The coincidence is further confirmed by Y-doped 2212 phase.

关键词： Sr Bi

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电解加工电源研究现状及发展趋势

机械工程学报 2021年第13期57卷 201-213页

作者：罗红平张清荣刘桂贤张永俊广东工业大学机电工程学院广州510006 广东工业大学广州市非传统制造技术及装备重点实验室广州510006 广东工业大学省部共建精密电子制造技术与装备国家重点实验室广州510006

电解加工电源是电解加工装备中的关键核心基础设备,其性能直接影响着电解加工的精度、稳定性、表面质量和经济性。为了更加充分发挥电解工艺的潜力和优势,实现精密、高效、稳定的电解加工,有必要对电解加工电源的研究现状及发展趋势进... 详细信息

电解加工电源是电解加工装备中的关键核心基础设备,其性能直接影响着电解加工的精度、稳定性、表面质量和经济性。为了更加充分发挥电解工艺的潜力和优势,实现精密、高效、稳定的电解加工,有必要对电解加工电源的研究现状及发展趋势进行系统梳理和全面总结。系统分析了宏观尺度电解加工和微细电解加工的工艺原理,加工质量、效率和工艺稳定性等的影响因素,归纳总结出不同类别、不同应用场合下电解工艺对加工电源的性能指标要求。较为全面地介绍了近年来国内外在电解加工电源方面的相关研究进展及应用情况。对电解加工电源在控制高频脉冲产生、信号处理与输出、加工间隙监测、电源保护等几个方面的关键技术进行了评述,同时对电解加工电源的发展趋势进行了预测和展望,为促进我国电解加工技术的快速发展和广泛应用提供有益的参考。

关键词：电解加工电源微细电解加工高频脉冲