Experimental verifications on chemical carcinogenesis, a bifunctional alkvlation between DNA interstrands

作     者：DAI Qianhuan, ZHANG Qingrong, WANG Lihui, PEI Shu, WANG Qing &QUBoCenter for Chemistry and Bioengineering of Cancer, Beijing Polytechnic University, Beijing 100022, China 

作者机构：Center for Chemistry and Bioengineering of Cancer Beijing Polytechnic University Beijing China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2000年第45卷第12期

页      面：1075-1079页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：National Natural Science Foundation of China  NSFC  (29'742002) 

主　　题：mechanism of chemical carcinogenesis di-region theory DNA interstrand cross-link aromatic amines poiycyclic aromatic hydrocarbons beryllium salt cadmium salt. 

摘      要：It is evidenced by the filter elution method that two carcinogenic aromatic hydrocarbons, benzo[a]pyrene and dibenzo[a,h]anthracene, two carcinogenic metal salts, beryllium chloride and cadmium chloride, four carcinogenic aromatic amines, 2-aminofluorene, p-naphthylamine, 4-aminobiphenyl and benzidine, can all induce DNA interstrand and DNA-protein cross-link in L12io culture. However, under the same condition, the corresponding non-carcinogenic compounds, including benzo[k]fluorancene, anthracene, magnesium chloride, zinc chloride, a-naphthylamine, 2-aminobiphenyl and m-toluidine, cannot produce any cross-link adducts. All these results are consistent with the di-region theory that carcinogens are bio-bifunctional alkylation agents. This method can also be used to discriminate carcinogens and non-carcinogens.